Q#1:

A validation process of a moist heat sterilizer was performed. Four certified data-
loggers (Probes) distributed within an empty cycle for 15 minutes at 121 °C. According
to the data-logger readings obtained for the distribution test (below), is the process
valid or not, why?

Probe
positio Temp, °C
n
At 119. 119. 119. 119. 119. 119. 119. 119. 120. 120. 121. 120. 120. 120. 120.
Front 0 0 2 4 4 6 8 8 0 0 3 3 3 5 5
Betwee
n 118. 118. 118. 119. 119. 119. 119. 119. 119. 119. 119. 119. 119. 120. 120.
Front- 5 5 7 0 0 2 4 4 6 6 8 8 8 0 0
Middle
Betwee
n 118. 118. 118. 118. 118. 118. 118. 119. 119. 119. 119. 119. 119. 119. 119.
Middle 2 4 4 6 6 9 9 1 1 3 3 3 5 5 5
-Back
118. 118. 119. 119. 119. 119. 119. 119. 119. 119. 120. 120. 120. 120. 120.
At Back
1 8 0 2 2 4 6 6 8 8 1 1 1 3 3

Q#2:

The data (below) were obtained from three probes distributed within a liquid cycle at
maximum load to assess the validity of a steam autoclave, the probe at the front
reading +0.57 °C, where the two others reading -0.22 °C "as the certificate for each
one". Consider the temperatures and calculate the compensation time for this cycle?
"the basic time cycle is 15 minutes at 123 °C "

Probe
Temp, °C,
position
120. 120. 120. 120. 120. 120. 121. 121. 121. 121. 121.
At Front 120.0 120.8 121.3 121.5
0 2 4 4 6 8 0 0 3 3 5
119. 119. 120. 120. 120. 120. 120. 120. 121. 121. 121.
At Middle 119.7 120.6 121.0 121.2
7 9 2 2 4 6 8 8 0 0 2
119. 120. 120. 120. 120. 121. 121. 121. 121. 121. 121.
At Back 120.3 121.1 121.6 121.8
6 5 7 7 9 1 3 3 6 6 8

Q#3:
Assume that you need to decide the time of sterilization cycle at 134 °C, what is the
exposure time you need for this cycle, explain?