Universidad Autónoma de Nuevo León

Preparatoria No.22

Anatomy and Physiology

Fernando Silva Mezquiti

Lab 1 (Catalase Action in Living Tissue)

4 Semester

Group 615

Jesus Orlando Garcia Arevalo

Roll Number 16

1483341

February 29, 2010

Catalase Action in Living Tissue

Introduction:

We saw a demonstration of the mix of H2O2 with different tissues, like

vegetal and living tissue, for see the different reaction in each one.

Objectives:

 Demonstrate the presence of catalase in Living Tissue.

 Observe the affect of catalase on hydrogen peroxide.

Materials:

 Magnifying class
 Wooden Stick
 12ml Syringe
 Rack with test tubes
 H2O2
 Potato cubes
 Liver cubes

Procedure:

1. Put the potato cubes in a test tube, and make it too with the liver
cubes.
2. Take the Wooden Stick and press the cubes to be comprised.
3. Take the H2O2 with the 12ml Syringe.
4. Put the H2O2 in a test tube.
5. Make the step three and four but put the H2O2 in the other one
tube.
6. Look what happen in the test tubes with the cubes.
Results:

In the test tube of potato cubes, happen a small reaction with the
H2O2, just appear little bubbles.

But in the test tube of liver cubes, happen a bigger reaction than
the other one, here appear more bubble and appear too fast.

Results of Catalase Activity in Living Tissue

Experiment Enzyme Substrate Product Observations

Catalase Catalase Hydrogen -Oxygen The reaction
from living peroxide -Water causes
tissue bubbles

Conclusion:

That reaction happened with more force in the liver because the liver is
where happen the detoxification, and the catalase is a product of the
liver, that is present to neutralize the peroxide.