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Copper, Bicinchoninate Method 8506, 02-2009, 9th Ed PDF
Copper, Bicinchoninate Method 8506, 02-2009, 9th Ed PDF
1. Enter the stored 2. Press: 20 ENTER 3. Fill a sample cell 4. Place the blank into
program number for The display will show with 10 mL of sample the cell holder. Tightly
bicinchoninate copper mg/L, Cu and the (the blank). cover the sample cell
(Cu)- powder pillows. ZERO icon. Note: Adjust the pH of with the instrument cap.
Press: PRGM Note: Determination of acid-preserved samples to
total copper needs a prior 4-6 with 8 N KOH before
The display will show: analysis. Do not exceed pH
digestion (see Digestion in
PRGM ? Section 2). 6 or copper may precipitate.
Note: For most accurate
results, perform a Reagent
Blank Correction using
deionized water (see
Section 1).
177
COPPER, continued
178
COPPER, continued
Using AccuVac Ampuls Method 8026
1. Enter the stored 2. Press: 20 ENTER 3. Fill a sample cell 4. Place the blank into
program number for The display will show with at least 10 mL of the cell holder. Tightly
bicinchoninate copper mg/L, Cu and the sample (the blank). cover the sample cell
(Cu)- AccuVac ampuls. ZERO icon. Collect at least 40 mL of with the instrument cap.
Press: PRGM sample in a 50-mL
Note: Determination of
total copper needs a prior beaker.
The display will show:
digestion (see Digestion in
PRGM ? Section 2).
Note: For most accurate Note: Adjust the pH of
results, perform a Reagent stored samples before
Blank Correction using analysis.
deionized water (see
Section 1).
179
COPPER, continued
180
COPPER, continued
Sampling and Storage
Collect samples in acid-cleaned glass or plastic containers.
Adjust the pH to 2 or less with nitric acid (about 2 mL per liter).
Store preserved samples up to six months at room temperature.
Before analysis, adjust the pH to 4 to 6 with 8 N potassium
hydroxide. Do not exceed pH 6, as copper may precipitate.
Correct the test result for volume additions; see Correction for
Volume Additions in Section 1 for more information. If only
dissolved copper is to be determined, filter the sample before acid
addition using the labware listed under Optional Apparatus.
Accuracy Check
Standard Additions Method
a) Fill three 25-mL graduated mixing cylinders with 25 mL of
sample.
181
COPPER, continued
and two representative lots of reagent with the instrument, a
single operator obtained a standard deviation of ±0.02 mg/L Cu.
Interferences
Interfering Substances and Suggested Treatments for Powder
Pillows
Interfering
Interference Level and Treatment
Substance
182
COPPER, continued
other complexing agents, use a Free Copper Reagent Powder
Pillow in place of the CuVer 1 pillow in Step 4. Results in Step
10 will be free copper only. Add a Hydrosulfite Reagent Powder
Pillow to the same sample and
re-read the result. This result will include the total dissolved
copper (free and complexed).
Interfering Substances and Suggested Treatments for AccuVac
Ampuls
Interfering
Interference Level and Treatment
Substance
Summary of Method
Copper in the sample reacts with a salt of bicinchoninic acid
contained in CuVer 1 or 2 Copper Reagent to form a purple
colored complex in proportion to the copper concentration. This
method includes procedures for both powder pillow and AccuVac
reagents.
183
COPPER, continued
REQUIRED REAGENTS & APPARATUS (Using Powder Pillows)
Quantity Required
Description Per Test Unit Cat. No.
CuVer 1 Copper Reagent Powder Pillows ...............1 pillow ............ ...100/pkg.......... 21058-69
Sample Cell, 10-20-25 mL, w/cap ................................ 2......................... 6/pkg.......... 24019-06
OPTIONAL APPARATUS
Description Unit Cat. No.
AccuVac Snapper Kit.................................................................................. each.......... 24052-00
Ampule Breaker Kit.................................................................................... each.......... 21968-00
Cylinder, graduated, mixing, 25 mL........................................................... each.......... 20886-40
Cylinder, graduated, polypropylene, 25 mL ............................................... each............ 1081-40
Cylinder, graduated, 100 mL ...................................................................... each.............. 508-42
Filter Paper, folded, 12.5 cm................................................................. 100/pkg............ 1894-57
Filter Pump ................................................................................................. each............ 2131-00
Flask, volumetric, 100 mL, Class A ........................................................... each.......... 14547-42
Funnel, polypropylene, 65 mm ................................................................... each............ 1083-67
Hot Plate, 4" diameter, 120 V ..................................................................... each.......... 12067-01
Hot Plate, 4" diameter, 240 V ..................................................................... each.......... 12067-02
pH Indicator Paper, 1 to 11 pH ......................................................... 5 rolls/pkg.............. 391-33
pH Meter, sension 1, with electrode .......................................................... each.......... 51700-10
Pipet, TenSette, 0.1 to 1.0 mL..................................................................... each.......... 19700-01
184
COPPER, continued
Pipet Tips, for 19700-01 TenSette Pipet .................................................50/pkg ..........21856-96
Pipet Tips, for 19700-01 TenSette Pipet .............................................1000/pkg ..........21856-28
Pipet, volumetric, Class A, 1.00 mL ...........................................................each ..........14515-35
Pipet Filler, safety bulb ..............................................................................each ..........14651-00
185