CELL EYE: A Cell Counter and Identifier
ABSTRACT individual cells in a sample, while ignoring noise.
This study documents the development of CELL EYE: A cell
counter and Identifier. There are different species of cell that
are living in the world. One these cells are microalgae it has
received considerable interest as a potential feed stock for
biofuel production because of the useful quantities. Obtaining
high yield and good quality of the said raw materials is must
in optimizing biofuel production. Therefore, daily monitoring
of algal growth is done manually by counting microalgae cells
under the microscope that is time consuming and tedious.
Thus, the proponents developed a system for automating the
method of counting microalgae cells is implemented by
Raspberry Pi to minimize the work and time of counting
which takes a lot of time in just counting the cells. Image of
the cells will bet taken under a low power objective
microscope. T-test will be used in the study to analyze
automated counting and F1 score of for the accuracy of the
system.
1. INTRODUCTION
Microalgae cell counting is necessary in determining the
number of cells in a milliliter of a liquid. It is done to estimate
the size and population of the cultured algae and the estimated
growth of life. The purpose of cell counting is for the
management of the cell cultures in the biological research. In
addition, cell counting is important for the monitoring of cell
health and proliferation rate.
Hemocytometry is the manual way of counting cells that uses
microscope and hemocytometer. This is the commonly used
method in cell counting. A hemocytometer is a special type of
microscopic slide consisting of two chambers, which is
divided into nine larges squares with each of its area of 1mm 2.
The central counting area contains 25 large squares each large
square has 16 smaller squares. The chamber is 0.1 mm in
height so that each square corresponds to a given volume,
applied to count each cell with a size of 2-30 micrometer and
concentrations of 104-107 cells per mL. The advantage of
using this is that includes the ability to make immediate
judgements is a term used to depict any sort of unmanned
decisions about the sample analysis. But cell counting using
hemocytometer suffers from a variety of shortcomings like,
lack of statistical robustness at low sample concentration,
poor count due to devise misuse, subjectivity of counts among
users. In addition, it is very time consuming and tedious
operation.
The current method for identifying, counting and measuring
cells are best semi-automatic but slow. This is due to the
complex nature of the microscopic images. Also, algal cells
are typically clustered and overlapping and a method is
needed for accurately separating, identifying and counting
Overcoming these problems using image analysis is the first
step in developing automatic figure methods of estimating
biomass directly from the microscopic image.
2. METHODS
To gather data on different conditions, experimental method
is conducted. It will be utilized to deal with encountered
problems and to maintain theoretical assumptions of the
proponents. Data gathered in this method will be used to
achieve the purpose of the study and to be able to make the
project more successful.
2.1. Methods of Research
In this design project, descriptive method and experimental
method of research were applied. Descriptive method of
research produces information about the current situation and
is concerned about answering questions through interviews or
questionnaires. The aim of the research is to gather
information for the different types of cells that will be tested.
Experimental method will be utilized to deal with
encountered problems and to maintain theoretical assumptions
of the proponents. The proponents will conduct series of
experiments including prototype testing and calibrations to
accomplish the purpose of the study. The proponents procured
data and information from research works, past studies, and
the internet to guide them in this study.
2.1.1.System Implementation
The proposed system will make used of a low microscope and
that will be used to seeing the cell count and the Raspberry Pi
for the camera and storage of the data that will be gather will
be stored in a low memory which is the sd card and also will
be connected in an available storage in the web to maximize
its capability.
Open CV is the application that will be installed in
the Raspberry Pi to be able to use the camera module for the
system. To be able for a clearing vision of the specimen and a
smooth recording of the data gathered in doing the study.
Figure 1: Block diagram of CELL EYE