labs

Sean Burke
Zane Walas
Simon Moattar
Nicholas Hadiaris

Cover Page...

Table of contents...

Abstract...

Introduction...

Theory...

Method...

References

UVC Testing

Hidden Contamination at Checkout: Grocery Conveyor Belts

KlēnLite – Supermarket conveyor sterilizer.

Appendix

Theory: In our design our group has been back and forth with the efficiency of using
either a spray mechanism or a uv light. We have been debating on three factors.
1.Efficiency- How fast it would take between the spray and the uv light to sanitize the
belt
2.Effectiveness- How effective in sanitizing the sanitizer is versus the uv light.
-How many different bacteria can they destroy?
-How quickly will they act?
-How thorough is each product?
3.Potential issues?- Would the spray create dangerous mold around the food area
overtime?
Does the UV give off any negative effects? Would the material of the belt be able to
withstand sustained exposure to UV-C light?

Big Focus Question-For our first experiment we focused on the effectiveness of each
method. Our big question was,
“Will using a spray sanitizer or a UV light be more effective in sanitizing a belt?”

Method:
Equipment
● 1 liter of J-512 sanitizer
● 1 UV-C light
● 18 petri dishes with agar
● Swabs
● 1 spray bottle
● A stopwatch
Procedure
1. Prepare 3 sets of four dishes with agar
2. Put J-512 sanitizer in a spray bottle
3. Prepare a stopwatch
4. Cough into two out of the first set of petri dishes
a. After both dishes have been coughed into, wait about ten seconds
and spray each dish once with the J-512 and start the stopwatch
b. Wait 15 seconds and then gently swab each petri dish
c. Contaminate the two fresh petri dishes with these swabs
5. Cough into two out of the second set of petri dishes
 a. After both dishes have been coughed into, wait about ten seconds
and spray each dish once with the J-512 and start the stopwatch
b. Wait 30 seconds and then gently swab each petri dish
c. Contaminate the two fresh petri dishes with these swabs
6. Cough into two out of the third set of petri dishes
a. After both dishes have been coughed into, wait about ten seconds
and spray each dish once with the J-512 and start the stopwatch
b. Wait 60 seconds and then gently swab each petri dish
c. Contaminate the two fresh petri dishes with these swabs
7. Prepare three pairs of two petri dishes
8. Make sure UV-C light is fully charged
9. Prepare a stopwatch
10. Cough into the first pair of petri dishes
a. After both petri dishes have been coughed into, wait about ten
seconds and turn on the UV-C light over each dish and start the
stopwatch
b. After 15 seconds of light on each petri dish, turn off the light
11. Cough into the second pair of petri dishes
a. After both petri dishes have been coughed into, wait about ten
seconds and turn on the UV-C light over each dish and start the
stopwatch
b. After 30 seconds of light on each petri dish, turn off the light
12. Cough into the third pair of petri dishes
a. After both petri dishes have been coughed into, wait about ten
seconds and turn on the UV-C light over each dish and start the
stopwatch
b. After 60 seconds of light on each petri dish, turn off the light
13. Store each of the second pairs of petri dishes from the J-512 portion as
well as all of the petri dishes from the UV-C portion in an undisturbed,
incubated area for 48 hours
a. Note growth of bacteria at this time

Images of Experimental Setup:

Incubation Box (top left) UV-C light (right) Petri dishes and sanitizer (bottom left)
Data:

Experiment Data J-512 petri dishes UV-C petri dishes

# Bacteria colonies (15 sec 4+ / 4+ (8+ avg) 2 / 6 (4 avg)

exposure)

# Bacteria colonies (30 sec 2 / 3 (2.5 avg) 3 / 2 (2.5 avg)

Exposure)

#Bacteria colonies (60 sec 4 / 3 (3.5 avg) 3 / 1 (2 avg)

exposure)
Second Experiment
Big Focus Question- “What materials will hold up the best in the conditions that our
product would be under?”

Method:
Equipment:
● Onshape 3D model
● Solidworks simulation

Procedure:
● Working on it