Biology O Level Paper 1 MCQs Topical Pas

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Title Biology O-Level Paper-1 Topical Unsolved (Article#201)

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CONTENTS
UNIT 1 ----------------------------------------------------------------------------- 4
1.1 Plant and animal cells ------------------------------------------------------------------------------------------- 4
1.2 Specialised cells, tissues and organs ----------------------------------------------------------------------- 8
1.3 Multiple Topics --------------------------------------------------------------------------------------------------- 10
Answers Section ---------------------------------------------------------------------------------------- 11
UNIT 2 -------------------------------------------------------- 12
2.1 Diffusion ----------------------------------------------------------------------------------------------------------- 12
2.2 Osmosis ----------------------------------------------------------------------------------------------------------- 13
2.3 Active transport --------------------------------------------------------------------------------------------------- 17
2.4 Multiple Topics ---------------------------------------------------------------------------------------------------- 20
Answers Section ---------------------------------------------------------------------------------------- 28
UNIT 3 ----------------------------------- 29
3.1 Enzyme action --------------------------------------------------------------------------------------------------- 29
3.2 Effects of temperature and pH ------------------------------------------------------------------------------- 34
3.3 Multiple Topics --------------------------------------------------------------------------------------------------- 37
Answers Section ---------------------------------------------------------------------------------------- 39
UNIT 4 ------------------------------------------------------------------------------------------------ 40
4.1 Photosynthesis --------------------------------------------------------------------------------------------------- 40
4.2 Leaf structure ----------------------------------------------------------------------------------------------------- 56
4.3 Mineral nutrition -------------------------------------------------------------------------------------------------- 65
4.4 Multiple Topics --------------------------------------------------------------------------------------------------- 68
Answers Section ---------------------------------------------------------------------------------------- 70
UNIT 5 ----------------------------------------------------------------------------- 71
5.1 Nutrients ----------------------------------------------------------------------------------------------------------- 71
5.2 Diet ------------------------------------------------------------------------------------------------------------------ 79
5.3 World food supplies -------------------------------------------------------------------------------------------- 80
5.4 Human alimentary canal -------------------------------------------------------------------------------------- 81
5.5 Chemical digestion ---------------------------------------------------------------------------------------------- 88
5.6 Absorption and assimilation ---------------------------------------------------------------------------------- 92
5.7 Multiple Topics --------------------------------------------------------------------------------------------------- 96
Answers Section ---------------------------------------------------------------------------------------- 101
UNIT 6 ----------------------------------------------------------------------------- 102

6.1 Water and ion uptake ------------------------------------------------------------------------------------------ 102
6.2 Transpiration and translocation ----------------------------------------------------------------------------- 110
6.3 Multiple topics --------------------------------------------------------------------------------------------------- 122
Answers Section ---------------------------------------------------------------------------------------- 128
UNIT 7 --------------------------------------------- 129

7.1 Circulatory system --------------------------------------------------------------------------------------------- 129
7.2 Multiple Topics --------------------------------------------------------------------------------------------------- 150
Answers Section ---------------------------------------------------------------------------------------- 158
UNIT 8 ----------------------------------------------------------------------------- 159

8.1 Aerobic respiration ---------------------------------------------------------------------------------------------- 159
8.2 Anaerobic respiration ------------------------------------------------------------------------------------------- 160
8.3 Human gas exchange ------------------------------------------------------------------------------------------ 164
8.4 Multiple Topics --------------------------------------------------------------------------------------------------- 178
Answers Section ---------------------------------------------------------------------------------------- 184
UNIT 9 -------------------------------------------------------------------------------------------------- - 185

9.1 Structure and function of kidneys --------------------------------------------------------------------------- 185
9.2 Kidney dialysis --------------------------------------------------------------------------------------------------- 190
9.3 Multiple Topics --------------------------------------------------------------------------------------------------- 195
Answers Section ---------------------------------------------------------------------------------------- 196
UNIT 10 ------------------------------------------------------------------ 197

10.1 Structure and function of the skin --------------------------------------------------------------------------- 197
10.2 Multiple Topics --------------------------------------------------------------------------------------------------- 201
Answers Section ---------------------------------------------------------------------------------------- 207
UNIT 11 ----------------------------------------------------------------------------- 208

11.1 Nervous system -------------------------------------------------------------------------------------------------- 208
11.2 Receptors --------------------------------------------------------------------------------------------------------- 217
11.3 Reflex action ------------------------------------------------------------------------------------------------------ 228
11.4 Hormones --------------------------------------------------------------------------------------------------------- 233
11.5 Multiple Topics --------------------------------------------------------------------------------------------------- 238
Answers Section ---------------------------------------------------------------------------------------- 239
UNIT 12 ----------------------------------------------------------------------------- 240

12.1 Bones -------------------------------------------------------------------------------------------------------------- 240
12.2 Joints ---------------------------------------------------------------------------------------------------------------- 244
12.3 Antagonistic muscles -------------------------------------------------------------------------------------------- 246
12.4 Multiple Topics --------------------------------------------------------------------------------------------------- 251
Answers Section ---------------------------------------------------------------------------------------- 255
UNIT 13 ----------------------------------------------------------------------------- 256

13.1 Antibiotics ---------------------------------------------------------------------------------------------------------- 256
13.2 Effects of heroin -------------------------------------------------------------------------------------------------- 258
13.3 Effects of alcohol ------------------------------------------------------------------------------------------------- 259
13.4 Effects of tobacco smoke -------------------------------------------------------------------------------------- 261
13.5 Multiple Topics --------------------------------------------------------------------------------------------------- 266
Answers Section ---------------------------------------------------------------------------------------- 268
UNIT 14 ----------------------------------------------------------------------------- 269

14.1 Microorganisms --------------------------------------------------------------------------------------------------- 269
14.2 Food biotechnology ---------------------------------------------------------------------------------------------- 274
14.3 Industrial biotechnology ---------------------------------------------------------------------------------------- 278
14.4 Multiple Topics ---------------------------------------------------------------------------------------------------- 283
Answers Section ---------------------------------------------------------------------------------------- 284
UNIT 15 ----------------------------------------------------------------------------- 285

15.1 Energy flow -------------------------------------------------------------------------------------------------------- 285
15.2 Food chains and food webs ----------------------------------------------------------------------------------- 289
15.3 Carbon cycle ------------------------------------------------------------------------------------------------------ 301
15.4 Nitrogen cycle ---------------------------------------------------------------------------------------------------- 306
15.5 Parasitism --------------------------------------------------------------------------------------------------------- 312
15.6 Effects of humans on the ecosystem ----------------------------------------------------------------------- 317
15.7 Pollution ----------------------------------------------------------------------------------------------------------- 321
15.8 Conservation ----------------------------------------------------------------------------------------------------- 325
15.9 Multiple Topics -------------------------------------------------------------------------------------------------- 326
Answers Section ---------------------------------------------------------------------------------------- 330
UNIT 16 ----------------------------------------------------------------------------- 331

16.1 Asexual reproduction -------------------------------------------------------------------------------------------- 331
16.2 Sexual reproduction in plants -------------------------------------------------------------------------------- 333
16.3 Sexual reproduction in humans ------------------------------------------------------------------------------ 352
16.4 Sexually transmitted diseases -------------------------------------------------------------------------------- 367
16.5 Multiple Topics --------------------------------------------------------------------------------------------------- 370
Answers Section ---------------------------------------------------------------------------------------- 373
UNIT 17 ----------------------------------------------------------------------------- 374

17.1Variation ------------------------------------------------------------------------------------------------------------ 374
17.2Chromosomes and DNA -------------------------------------------------------------------------------------- 377
17.3Monohybrid inheritance ---------------------------------------------------------------------------------------- 379
17.4Selection ----------------------------------------------------------------------------------------------------------- 393
17.5Genetic engineering -------------------------------------------------------------------------------------------- 395
17.6Multiple Topics --------------------------------------------------------------------------------------------------- 397
Answers Section ---------------------------------------------------------------------------------------- 399
YEARLY PAST PAPER --------------------------------------------------------------------------------------------- 400
May/June 2018 Paper-12 ----------------------------------------------------------------------------- 400
Answers Section ---------------------------------------------------------------------------------------- 413
October/November 2018 Paper-12 ------------------------------------------------------------------- 414
Answers Section ---------------------------------------------------------------------------------------- 425
May/June 2019 Paper-12 ----------------------------------------------------------------------------- 426
Answers Section ---------------------------------------------------------------------------------------- 436
Biology O-Level Paper-1 Classified 4 1.1 Plant and animal cells
Read & Write Publications
Unit 1: Cell structure and organisation

1.1 Plant and animal cells
1. O/N 16/P12/Q1
The diagram shows a plant cell.
Which two structures are not found in animal cells?

A 1 and 2 B 2 and 3 C 3 and 4 D 4 and 5
2. M/J 16/P11/Q1
Which two structures are found in all plant epidermal cells?
A cell wall and chloroplasts C chloroplasts and starch grains
B cell wall and nucleus D nucleus and starch grains
3. M/J 15/P12/Q1
Which structure identifies this as a plant cell rather than an animal cell?
A cell membrane B cell wall C cytoplasm D nucleus
4. O/N 15/P12/Q1
Which diagram shows the positions of the chloroplasts and the nucleus in a cell?
A C
B D
5. M/J 14/P12/Q1
What are found in plant cells but not in animal cells?
1 cell membrane
2 nucleus
3 cell wall
4 chloroplast
6. M/J 12/P12/Q1
Some structures found in cells are listed.
1 cell wall
2 cell membrane
3 chloroplast
4 cytoplasm
5 nucleus
Which structures are found in both animal cells and plant cells?
A 1 2 4 C 2 3 5
B 1 2 3 D 2 4 5
7. M/J 12/P11/Q1
What is found in both animal and plant cells?
A cellulose cell wall C starch grain
B chloroplast D vacuole or vacuoles
8. O/N 11/P12/Q1, O/N 11/P11/Q1

A human cheek cell and a spongy mesophyll cell from a leaf are examined under a microscope.
Which structures are seen in both cells?
A cell membrane, nucleus and cytoplasm C cytoplasm, cell wall and cell membrane
B cell wall, cell membrane and nucleus D nucleus, cytoplasm and cell wall
9. M/J 11/P12/Q1, M/J 11/P11/Q1
Which features are not found in animal cells?

10. O/N 09/P1/Q2

The diagram represents a cell as seen under the electron microscope.
What type of cell is this?
11. M/J 09/P1/Q1

Which structure controls the passage of substances into and out of the cell?
12. O/N 07/P1/Q1
When a red stain is added to a culture containing both living and dead cells, only the dead cells take up the stain.
Which structure prevents the stain entering the living cells?
A cell membrane B cell wall C cytoplasm D vacuole
13. O/N 05/P1/Q1

Which labelled structure controls the passage of substances into and out of the cell?
14. M/J 03/P1/Q1

The diagram shows a cell.
Which numbers show the parts named?
15. O/N 02/P11/Q1

Which structures are found in animal cells and in plant cells?
A cell membrane and chloroplasts C cell wall and chloroplasts
B cell membrane and nucleus D cell wall and nucleus
Biology O-Level Paper-1 Classified 8 1.2 Specialised cells, tissues and organs
1.2 Specialised cells, tissues and organs

16. M/J 16/P12/Q3
The diagrams show a cylindrical net packed with rubber balloons full of air. The structure is used by a teacher to explain
wilting.
What is represented by the parts of the structure shown?
17. O/N 14/P12/Q1

The diagram shows three cellular structures.
Which statements about these cells are correct?
18. M/J 14/P11/Q1, M/J 02/P11/Q1

Which of the following have both cytoplasm and cell walls?
A liver cells B red blood cells C root hair cells D xylem vessels
Biology O-Level Paper-1 Classified 9 1.2 Specialised cells, tissues and organs
19. M/J 13/P12/Q1, M/J 13P11/Q1
Which structures are present in a root hair cell?
20. M/J 10/P12/Q11, M/J 10/P11/Q1

The photomicrograph shows onion epidermis.
Which term describes this onion epidermis?

A cell B organ C organ system D tissue
21. M/J 07/P1/Q1

The diagram shows three different cells, not drawn to the same scale.
Which are animal cells?

A 1 and 2 only B 1 and 3 only C 2 and 3 only D 1, 2 and 3
22. O/N 06/P1/Q1

The table shows some characteristics of four types of cell.
Which cell could be a root hair cell?
Biology O-Level Paper-1 Classified 10 1.3 Multiple Topics
1.3 Multiple Topics

23. O/N 16/P11/Q1
Which row correctly shows the function of a cell structure and its location?
11
Answers Section
1. A
2. B
3. B
4. C
5. D
6. D
7. D
8. A
9. B
10. A
11. B
12. A
13. B
14. B
15. B
16. B
17. C
18. C
19. B
20. D
21. C
22. B
23. A
Biology O-Level Paper-1 Classified 12 2.1 Diffusion
Unit 2: Diffusion and Osmosis

2.1 Diffusion
1. M/J 17/P12/Q2
Which statements about diffusion are correct?
1 Molecules move at random.
2 Molecules move down a concentration gradient.
3 Molecules may move through a partially permeable membrane.
A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
2. O/N 16/P11/Q2
Which row describes diffusion into a cell?
3. O/N 15/P11/Q2
What describes the diffusion of molecules?
A movement from a region of their higher concentration to a region of their lower concentration down
a concentration gradient
B movement from a region of their higher concentration to a region of their lower concentration up a
concentration gradient
C movement from a region of their lower concentration to a region of their higher concentration down
a concentration gradient
D movement from a region of their lower concentration to a region of their higher concentration up a
concentration gradient
4. M/J 14/P12/Q2
Which statement is always correct when oxygen is diffusing out of a plant cell?
A The concentration of carbon dioxide is higher inside the cell than outside.
B The concentration of carbon dioxide is higher outside the cell than inside.
C The concentration of oxygen is higher inside the cell than outside.
D The concentration of oxygen is higher outside the cell than inside.
Biology O-Level Paper-1 Classified 13 2.2 Osmosis
2.2 Osmosis
5. O/N 16/P12/Q3, O/N 16/P11/Q3, O/N 13/P12/Q2, O/N 13/P11/Q1
The diagram represents apparatus used to investigate osmosis.
Which molecules will move across the partially permeable membrane and which change will occur in the solution level?
6. O/N 15/P12/Q3
The diagram shows the net movement of water by osmosis between four adjacent cells.
Which cell has the highest water potential?
7. M/J 14/P11/Q2
Plant cells are placed in a solution with a lower water potential than that of the cells.
Which row is correct?
8. M/J 15/P12/Q3, M/J 15 P11/Q3, M/J 12/P11/Q2

The diagram shows three plant cells labelled P, Q and R. The arrows show the direction of water movement by osmosis.
What is the correct order of water potential in the cells, from the highest to the lowest?
9. M/J 10/P12/Q5, M/J 10/P11/Q3

Which of these processes require energy from respiration?
10. M/J 14/P12/Q3
The diagram represents the passage of water molecules and glucose molecules across a partially permeable cell surface
membrane.
Which arrow indicates osmosis?
11. M/J 12/P12/Q3, M/J 12/P11/Q3, M/J 10/P12/Q4, M/J 10/P11/Q2

Identical pieces of potato are placed in sucrose solutions of different concentrations. After three hours, the mass of each
potato piece is measured.
Which graph shows the results of this experiment?
A C
B D
12. O/N 08/P1/Q2
Diagram 1 shows an onion cell that has been placed in pure water.
The cell is now placed in a concentrated sugar solution.

It changes to appear as in diagram 2.
Which statement explains the change?

A Sugar has moved into the cell. C Water has moved into the cell.
B Sugar has moved out of the cell. D Water has moved out of the cell.
13. O/N 06/P1/Q3

The diagram shows apparatus used to investigate osmosis.
Which molecules will move across the partially permeable membrane and which changes in levels will occur?
Biology O-Level Paper-1 Classified 17 2.3 Active transport
2.3 Active transport

14. O/N 17/P11/Q2
Which movement of a substance in plants is an energy-consuming process?
A absorption of carbon dioxide by a palisade cell
B loss of water into the air spaces of a leaf
C nitrate uptake by root hair cells
D transport of water up through the xylem
15. M/J 17/P12/Q3

Which row defines active transport?
16. O/N 16/P12/Q2

The rate of nitrate ion absorption by a root hair cell was measured at different soil nitrate
concentrations.
At X, the concentration of nitrate in the soil is the same as in the cell.
Which graph shows how the rate of absorption varies with nitrate concentration in the soil?
A C
B D
17. M/J 16/P11/Q3, O/N 13/P12/Q2, O/N 13/P11/Q3,
The small intestine of a person contains a lower concentration of glucose than is present in the
blood.
The cells of the villi absorb glucose.
By which process is the glucose absorbed?
A by active transport against the concentration gradient
B by active transport down the concentration gradient
C by diffusion against the concentration gradient
D by diffusion down the concentration gradient
18. O/N 15/P12/Q2

How do plants absorb nitrate ions by active transport?
A against a concentration gradient using energy
B against a concentration gradient without using energy
C down a concentration gradient using energy
D down a concentration gradient without using energy
19. O/N 15/P11/Q3

The diagram shows the concentration of magnesium ions in a healthy root hair cell of a plant and in the soil water
surrounding it.
For the plant to remain healthy, how will the magnesium ions move?
A into the cell by active transport C out of the cell by active transport
B into the cell by diffusion D out of the cell by diffusion
20. M/J 14/P11/Q3

Which diagram illustrates the process of active transport?
A C
B D
21. O/N 03/P1/Q3
The table shows the concentration of a substance inside and outside four different cells.
Which cell would need the most energy to absorb the substance by active transport?
22. O/N 02/P1/Q3

The diagram shows two plant cells, X and Y.
Cell X has a higher water potential than cell Y.
In which direction and by what process will water move between these two cells?
2.4 Multiple Topics

23. O/N 17/P11/Q3
The diagram shows the direction of water movement from one cell to another in a plant root.
Which cell has the higher water potential, and how does the water move?
24. O/N 17/P12/Q3

A student takes a potato and cuts three pieces from it. Each piece is 5 cm × 0.5 cm × 0.5 cm. He
places the three potato pieces into three different sugar solutions.
After two hours, he removes the potato pieces from the sugar solutions and measures their
lengths.
The results are shown in the table.
What can be concluded from these results?

A Solution Y has a lower water potential than the potato.
B Solution Z has the lowest water potential.
C The potato piece in solution X increases in length because it takes up sugar.
D The potato piece in solution Y decreases in length because it loses sugar.
25. M/J 17/P11/Q2

Which of these processes will always occur down a concentration gradient?
26. M/J 17/P11/Q3
A student cuts out four cylinders of potato. Each cylinder is 30 mm long. The cylinders are all of
the same diameter.
The potato cylinders are placed in sugar solutions of different concentrations. After one hour, the
lengths of the cylinders are measured again. The results are shown in the table.
Which sugar solution has a water potential closest to that of the potato cells?
27. O/N 16/P11/Q5

How do carbon dioxide and water enter a leaf?
28. M/J 16/P11/Q2

Which processes can only occur through a membrane?
29. M/J 15/P12/Q2, M/J 15 P11/Q2, O/N 12/P11/Q2

Which processes are responsible for the uptake of ions from the soil by a plant and the uptake of glucose into the villi of
a human?
30. M/J 15 P11/Q1
The cell wall of a plant cell is removed using an enzyme.
What would happen if this cell is then placed in distilled water?
A It would take longer for the cell to become turgid.
B Proteins in the cytoplasm would leave through the cell membrane.
C The cell would become smaller as water passes out.
D The cell would burst as water moves into it.
31. O/N 14/P12/Q3, O/N 14/P11/Q3

A cube of fresh potato is weighed. It is then placed in a test-tube containing a dilute solution of sucrose. After an hour, its
mass has increased.
Which process has occurred and what has happened to the concentration of the sucrose in the
solution in the test-tube?
32. O/N 14/P12/Q2

Active transport, diffusion and osmosis are described below.
1 the movement of ions or molecules across the cell membrane against a
concentration gradient using energy
2 the movement of ions or molecules from a region of high concentration to a
region of low concentration down a concentration gradient
3 the movement of water molecules from a region of their higher concentration
to a region of their lower concentration through a partially permeable
membrane
What links the descriptions with their names?
33. O/N 14/P11/Q2

The list shows the absorption of three different substances by organisms.
1 mineral ions entering a root hair cell from the soil
2 oxygen entering the blood from an alveolus
3 water entering a root hair cell from the soil
In each case, what is the mechanism of absorption?
34. O/N 11/P12/Q3, O/N 11/P11/Q3, O/N 07/P1/Q3
The mass of a cube of fresh potato is found. It is then placed in a test-tube containing a dilute
solution of sucrose. After an hour, its mass has increased.
Which process has occurred and what has happened to the concentration of the sucrose in the
solution in the test-tube?
35. M/J 11/P12/Q3, M/J 11/P11/Q2

The sentence describes the uptake of water by a plant.
Water moves into the root hairs of a plant by osmosis through a .....1..... permeable cell membrane, .....2..... a water
potential gradient.
Which words correctly complete gaps 1 and 2?
36. O/N 10/P12/Q14, O/N 10/P11/Q3, M/J 02/P1/Q3

Four identical pieces of potato are treated in two stages, as shown.
Which piece of potato will be largest after 2 hours?
37. M/J 08/P1/Q1

The diagram shows a typical plant cell after being placed in a concentrated salt solution for ten minutes.
Which numbered structures are partially permeable?

A 1 and 2 only B 1 and 3 only C 1 only D 2 only
38. M/J 09/P1/Q3
The arrows represent the movement of salts into a root hair cell.
What describes the movement of the salts?

A active transport against the concentration gradient
B active transport down the concentration gradient
C diffusion against the concentration gradient
D diffusion down the concentration gradient
39. M/J 07/P1/Q3

Five pieces are cut from a potato, all of equal size and shape. The pieces are then placed in sugar solutions of different
concentrations. After four hours, the change in length of each potato piece is measured.
The results are shown in the graph.
Which concentration of sugar solution has approximately the same water concentration as the potato?
A 0.00 mol dm–3 B 0.15 mol dm–3 C 0.30 mol dm–3 D 0.40 mol dm–3
40. O/N 05/P1/Q2

The diagram shows a root hair, surrounded by a dilute solution of mineral ions.
Which statement is correct?

A Water molecules move into the root hair because their concentration is lower inside.
B Water molecules move into the root hair because their concentration is lower outside.
C Water molecules move out of the root hair because their concentration is lower inside.
D Water molecules move out of the root hair because their concentration is lower outside.
41. M/J 06/P1/Q3
The diagram shows cells in fresh blood and the same cells after the blood has been mixed with liquid X.
Which statement describes the water potential of liquid X?

A It is equal to that of pure water. C It is higher than that of the cell cytoplasm.
B It is equal to that of the cell cytoplasm. D It is lower than that of the cell cytoplasm.
42. O/N 04/P1/Q3

A human red blood cell is placed in a strong salt solution.
In which direction does water move and what is the effect on the cell?
43. M/J 04/P1/Q3

Similar shaped pieces of potato are placed in sucrose solutions of different concentrations. After three hours, the mass of
each potato piece is measured.
Which graph shows the results of this experiment?
A C
B D
44. O/N 03/P1/Q7
The diagram shows cells in fresh blood, after the blood is diluted with a liquid.
Which statement describes the liquid?

A The water potential of the liquid is equal to that of pure water.
B The water potential of the liquid is equal to that of the cytoplasm.
C The water potential of the liquid is higher than that of the cytoplasm.
D The water potential of the liquid is lower than that of the cytoplasm.
45. O/N 03/P1/Q2

Four strips are cut from a fresh potato. The length of each strip is measured. One strip is placed in water, the others in
different concentrations of sugar solution.
After an hour, the strips are measured again. The results are shown in the table.
Which liquid is water?
46. M/J 03/P1/Q3

Cylinders of potato tissue were placed in different concentrations of a sugar solution. The graph shows the percentage
change in length of the cylinders of potato tissue.
Which solution has the same water potential as the potato tissue?
A 0.0 mol B 0.2 mol C 0.3 mol D 0.5 mol
47. M/J 03/P1/Q2
The arrows show the movement of salts into a cell.
Which describes the movement of the salts?

A active transport against the concentration gradient
B active transport down the concentration gradient
C diffusion against the concentration gradient
D diffusion down the concentration gradient
28
Answers Section
1. A 46. C
2. C 47. A
3. A
4. C
5. D
6. C
7. C
8. C
9. D
10. A
11. D
12. D
13. D
14. C
15. D
16. A
17. A
18. A
19. A
20. A
21. D
22. B
23. B
24. A
25. C
26. B
27. B
28. C
29. A
30. D
31. D
32. C
33. B
34. D
35. C
36. D
37. D
38. A
39. B
40. A
41. D
42. C
43. B
44. D
45. B
Biology O-Level Paper-1 Classified 29 3.1 Enzyme action
Unit 3: Enzymes
3.1 Enzyme action
1. M/J 17/P12/Q4
Which property of enzymes is explained by the lock and key hypothesis?
A All enzymes are proteins.
B Enzymes are inactive at very low temperatures.
C Human enzymes are most active just below 40 C.
D Most enzymes can only catalyse one reaction.
2. M/J 17/P11/Q4
Which are properties of enzymes?
3. O/N 16/P11/Q4
The diagram represents an enzyme molecule.
What could be substrates for this enzyme?
A W only B W or X C Y only D Y or Z
4. O/N 13/P12/Q4
In an enzyme action, where is the active site and where are the lock and the key?
5. O/N 15/P12/Q4
The diagram represents an enzyme and four molecules, A, B, C and D.
Which molecule is the substrate of this enzyme?
6. O/N 14/P11/Q4
The diagram illustrates the ‘lock and key’ hypothesis of enzyme action.
What are the enzyme, product and substrate in this reaction?
7. O/N 13/P11/Q4
Which statements are correct for all enzymes?
1 They are proteins.
2 They are secreted into the alimentary canal.
3 They speed up biochemical reactions.
4 None of them work at low pH.
8. O/N 12/P12/Q4, O/N 12/P11/Q4, O/N 08/P1/Q4, O/N 04/P1/Q4
According to the lock and key hypothesis, which is the lock and which is the key for the enzyme lipase?
9. M/J 09/P1/Q4
Four test-tubes containing egg white are incubated for ten minutes at 35 oC. Different substances are then added to the
four test-tubes.
In which test-tube is the egg white first digested?
10. M/J 08/P1/Q4

Enzyme action can be explained by the lock and key hypothesis.
Where is the active site and which acts as the lock or key?
11. M/J 07/P1/Q4

A dish is filled with agar jelly containing protein. Four holes are cut in the jelly and each hole is filled as shown. Pepsin is
a protease found in the stomach.
After 30 minutes, which hole will be surrounded by the largest area without protein?
12. O/N 07/P1/Q4
Which statement about enzymes is essential to the lock and key hypothesis?
A Enzyme molecules are catalysts.
B Enzyme molecules can be damaged by high pH values.
C Enzyme molecules can be damaged by high temperatures.
D Enzyme molecules each have a distinct shape.
13. O/N 05/P1/Q10

The diagram represents stages in the breakdown of starch to maltose by the enzyme amylase.
Which are the correct labels?
14. O/N 04/P1/Q10

Four bags made of partially permeable membrane are placed in tubes as shown in the diagram.
After 20 minutes at 35 C a sample of water from each tube, outside the bag, is boiled with Benedict’s solution.
What are the results?
15. O/N 02/P1/Q5
Four test-tubes are set up as shown.
All four tubes are then placed in a water-bath at 37 oC for 20 minutes.

What is the result?
16. O/N 02/P1/Q4

Which statement about enzymes is correct?
A They are all proteins.
B They are changed by the chemical reactions they control.
C They are destroyed by temperatures below 10 oC.
D They are found only in the alimentary canal.
17. M/J 02/P1/Q5

The diagram represents a model of enzyme action.
Which terms correctly identify X, Y and Z?

Biology O-Level Paper-1 Classified 34 3.2 Effects of temperature and pH
3.2 Effects of temperature and pH

18. M/J 14/P12/Q4
The diagram represents the ‘lock and key’ mechanism of an enzyme that works best at pH 7.
What shows the enzyme and its substrate at pH 13?
19. M/J 14/P11/Q4

A bacterium lives in hot springs at temperatures of 75oC to 85oC.
Which graph represents the activity of enzymes found in these bacteria?
A C
B D
20. M/J 13/P11/Q4
The diagram represents how an enzyme molecule changes in shape.
What explains this change?

A It has been cooled to 5 oC.
B It has been heated to 70 oC.
C It has been placed in a concentrated salt solution.
D It has been placed in a dilute salt solution.
21. O/N 10/P12/Q13, O/N 10/P11/Q4

Which graph shows the effect of temperature on enzyme-controlled reactions?
A C
B D
22. O/N 06/P1/Q4
The diagrams represent an enzyme molecule and its substrate.
Which diagram shows these molecules after they are heated to 100 oC?
A C
B D
23. O/N 03/P1/Q5

The graph shows the effect of temperature on a chemical reaction which is controlled by enzymes.
At which point are most product molecules being released?
24. M/J 02/P1/Q4

The graph shows the effect of pH on the activity of four different enzymes.
Which pair of enzymes includes one from the stomach and one that is not affected by pH?
A P and Q B P and S C R and Q D R and S
3.3 Multiple Topics

25. O/N 17/P12/Q4, O/N 17/P11/Q4
According to the lock and key hypothesis, what is the lock and what is the key for the enzyme
lipase?
26. M/J 15/P12/Q4, M/J 15 P11/Q4, M/J 11/P12/Q5, M/J 11/P11/Q4,

A dish is filled with agar jelly containing starch. Four holes are cut in the jelly and each hole is
filled as shown.
After 30 minutes, which hole will be surrounded by the largest area without starch?
27. M/J 13/P12/Q4

Four test tubes, each containing 2 cm3 of amylase solution are treated as follows:
1 boiled, then cooled to 1 oC
2 boiled, then cooled to 25 oC
3 frozen, then warmed to 1 oC
4 frozen, then warmed to 25 oC
10 cm3 of starch solution were then added to each tube and after 5 minutes, 2 drops of iodine
solution were added to each tube.
Which row shows the results?
39
Answers Section
1. D
2. A
3. D
4. A
5. A
6. B
7. A
8. D
9. B
10. A
11. B
12. D
13. A
14. B
15. C
16. A
17. C
18. C
19. D
20. B
21. C
22. C
23. C
24. A
25. D
26. A
27. A
28. C
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Biology O Level Paper 1 MCQs Topical Pas

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Title Biology O-Level Paper-1 Topical Unsolved (Article#201)

UNIT 6 ----------------------------------------------------------------------------- 102

UNIT 7 --------------------------------------------- 129

UNIT 8 ----------------------------------------------------------------------------- 159

UNIT 9 -------------------------------------------------------------------------------------------------- - 185

UNIT 10 ------------------------------------------------------------------ 197

UNIT 11 ----------------------------------------------------------------------------- 208

UNIT 12 ----------------------------------------------------------------------------- 240

UNIT 13 ----------------------------------------------------------------------------- 256

UNIT 14 ----------------------------------------------------------------------------- 269

UNIT 15 ----------------------------------------------------------------------------- 285

UNIT 16 ----------------------------------------------------------------------------- 331

UNIT 17 ----------------------------------------------------------------------------- 374

Unit 1: Cell structure and organisation

Which two structures are not found in animal cells?

8. O/N 11/P12/Q1, O/N 11/P11/Q1

Which features are not found in animal cells?

10. O/N 09/P1/Q2

What type of cell is this?

11. M/J 09/P1/Q1

13. O/N 05/P1/Q1

14. M/J 03/P1/Q1

Which numbers show the parts named?

15. O/N 02/P11/Q1

1.2 Specialised cells, tissues and organs

What is represented by the parts of the structure shown?

17. O/N 14/P12/Q1

Which statements about these cells are correct?

18. M/J 14/P11/Q1, M/J 02/P11/Q1

20. M/J 10/P12/Q11, M/J 10/P11/Q1

Which term describes this onion epidermis?

21. M/J 07/P1/Q1

Which are animal cells?

22. O/N 06/P1/Q1

1.3 Multiple Topics

Unit 2: Diffusion and Osmosis

8. M/J 15/P12/Q3, M/J 15 P11/Q3, M/J 12/P11/Q2

9. M/J 10/P12/Q5, M/J 10/P11/Q3

11. M/J 12/P12/Q3, M/J 12/P11/Q3, M/J 10/P12/Q4, M/J 10/P11/Q2

The cell is now placed in a concentrated sugar solution.

Which statement explains the change?

13. O/N 06/P1/Q3

2.3 Active transport

15. M/J 17/P12/Q3

16. O/N 16/P12/Q2

18. O/N 15/P12/Q2

19. O/N 15/P11/Q3

20. M/J 14/P11/Q3

22. O/N 02/P1/Q3

2.4 Multiple Topics

24. O/N 17/P12/Q3

What can be concluded from these results?

25. M/J 17/P11/Q2

27. O/N 16/P11/Q5

28. M/J 16/P11/Q2

29. M/J 15/P12/Q2, M/J 15 P11/Q2, O/N 12/P11/Q2

31. O/N 14/P12/Q3, O/N 14/P11/Q3

32. O/N 14/P12/Q2

33. O/N 14/P11/Q2

35. M/J 11/P12/Q3, M/J 11/P11/Q2

36. O/N 10/P12/Q14, O/N 10/P11/Q3, M/J 02/P1/Q3

37. M/J 08/P1/Q1