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Schedule: Date:

Manual Pipetting: Practical Quiz Criteria

Procedure Corresponding point/s Remarks

1. Select the correct serologic pipette 1
2. Non dominant hand for holding the serologic pipette 1
3. Dominant hand for holding the rubber pipettol 1
4. Immerse the tip of the serologic pipette 2
approximately 1-2 inches in the solution in an
upright position.
5. Press the rubber pipettol and fit onto the 1
mouthpiece of the serologic pipette.
6. Release the pressure on the bulb in a control and 2
slow manner.
7. Aspirate solution over the “0” mark. 2
8. Quickly remove the pipettol and seal the 2
mouthpiece of the serologic pipette using the index
finger.
9. Raise the pipette out from the solution. 1
10. Wipe the excess fluid on the outside surface of the 2
immersed part of serologic pipette using a tissue
paper.
11. At eye level, adjust the lower meniscus of the liquid 3
exactly at the “0” mark.
12. Drain the liquid into the receiving container in a 2
vertical position and tip against the wall of the
receiving container.

Prepared by: JDPA, RMT