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Fast facts: Inline Dialysis for Professional IC
instruments

01 Basics:
Complex matrices, such as waste water, emulsions, soil
Benefits of the Inline Dialysis:
• protects column from large molecules, oily
eluates, dairy products and others will quickly foul or clog phases, proteins and particles
the column if injected directly. With the patented inline • no time-consuming manual sample preparation
«stopped flow dialysis» undesired matrix components necessary and no contamination by the
from a variety of demanding sample types can be elimi- operator
nated automatically. • low maintenance (sporadic replacement of
dialysis membrane after 40-400 samples)
Working principle: • combination with automated Inline Dilution
For simple inspection the dialysis cell consists of two possible
transparent parts that are screwed together and hold a • low cost of ownership
membrane between them. One two-channel peristaltic
pump is used to transport the sample and a second two-
channel peristaltic pump is used for the acceptor solu-
tion. The patented stopped flow status during the actual
dialysis is achieved by stopping the pump on the accep-
tor side for a defined dialysis time, while the sample is
continuously transported along the other side of the
mem­­brane. Therefore, the sample concentration on the
sample side of the membrane remains constant. The in­­
ac­tive two-channel peristaltic pump ensures that both
inlet and outlet of the acceptor side are blocked during
that time. Due to the osmotic effect the concentration of
all substances that can pass through the membrane is
identical on both sides after typically 8-14 minutes. Then
the pump on the acceptor side is started to transfer the
dialysate to the injection valve of the IC system. Inline
Dialysis of the next sample can take place during the
chromatographic run of the current sample, providing
time efficient sample prepraration.

Product of competitions: Arguments against Thermo's AS-DV / AS-AP:

None, Inline Dialysis for IC is a unique technique only Thermo's autosamplers can only filtrate samples and is
available from Metrohm. therefore not suitable for samples with e.g. an oily matrix
or pro­­­­teins.

Possible argument of the competition: Metrohm counter arguments:

Carry-over between samples is a problem, because the
same membrane is used for several samples.
Carry-over originating from previous samples is prevent-
ed by rinsing the cell with ultrapure water. Furthermore,
the sample itself is used to purge the membrane and
tubing. Carry-over was found to be negligible in several
application works (<0.2%).

The Metrohm Inline Dialysis is rather a filtration than a
dialysis.
The Metrohm Inline Dialysis is patented and would not
have been patentable if it was just a filtration.
Filtration requires an actual flow through the filter
membrane, which is prevented by the fact that both inlet
and outlet of the acceptor side are blocked during the
dialysis.
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Example Applications:
Typical applications include, besides others, fruit juices, dyestuff. Find below a selection of application works
heavy waste water, cutting oil emulsions, milk products, using Inline Dialysis. Many more are available in the appli-
wine, emulsions, produ­ced water, cooling lubricants and cation database.

02 «Nitrite, nitrate analysis in salty meat using UV/VIS detection, Inline Dialysis and
Metrosep A Supp 10 - 250/4.0» (AW IC CH6-1208-082014)
«Sugar analysis in lactose-free milk using the amperometric detector and
Metrosep Carb 2 - 150/4.0» (AW IC CH6-1196-032014)
«Determination of nitrite and nitrate in buttermilk and cream powder applying
Inline Dialysis and Dose-in-Gradient» (AW IC DE8-0850-122013)
«Determination of Ethanolamine, Diethanolamine, Dimethylamine, Triethanolamine
and Diethylamine in Cosmetics by non suppressed conductivity detection» (AW IC CN6-0041-082013)
«Determination of sulfate in produced water» (AW IC CH6-0991-052009)
«Determination of chloride, saccharose, galactose, glucose, lactose, fructose and
maltose in lactose-free milk, cheese and cookies using the inline dialysis technique» (AW IC DE8-0816-122012)
«Determination of lactose in lactose-free food and beverages» (AW IC SW6-0001-052012)
«Malate, ascorbate, citrate and isocitrate in fruite juice after dialysis and
chemical supression» (AW IC CH6-0798-032004)
«Aliphatic monocarboxylic acids in produced water (oil industry) after dialysis
with MS detection» (AW IC CH6-0836-052005)
«Determination of fluoride, phosphate and sulphate in polymer samples
(for construction) using dialysis with chemical suppression» (AW IC CH6-0845-082005)
«Determination of anions in dyestuffs applying Inline Dialysis» (AW IC DE8-0629-062006)
«Determination of chloride, nitrite, nitrate, and sulfate in cooling lubricants and
cleaning solutions mixed with water» (AW IC DE8-0089-062008)
«Anion analysis in cutting oils with the compact dialysis setup» (AW IC CH6-0951-082008)

For the Compact IC instruments alternative setups are Order Information:

available. Details are described in the respective applica- For a new system and for upgrading existing systems two
tion works. Also available in combination with UV/Vis, unused two-channel peristaltic pumps (e.g. at autosam-
amperometric detection and MS. pler or at the instrument) must exist. All additionally
needed parts are in the dialysis kit 6.5330.100.

Furthermore, the following ProfIC systems with Inline

Dialysis are available:

940 with Inline Dialysis

ProfIC Vario 3 Anion and Cation

940 + Inline Dilution and Dialysis

ProfIC Vario 7 Anion and Cation

The following membranes are available:
cellulose acetate 6.2714.010 and polyamide 6.2714.030.

www.metrohm.com
8.108.5005EN