Hints for Hill Reaction Lab

Title- The Hill reaction in isolated chloroplasts

Aim: List about 3 main activities that were done.
Introduction:
● General description of photosynthesis:
Show chemical equation summarising process, location and maybe a
drawing of the chloroplast
● Focus on hill rxn: Light/Dark rxn.
● Description/Principle behind method
● Z-scheme
Method: Same point as previously stated in other labs.
Results:
● Part A. Determination of chlorophyll content.
Calculation of total yield - Using A = Ecl
1 ml of diluted chloroplasts contains 0.0025 mg chlorophyll
10 ml of diluted chloroplasts contains 0.025 mg chlorophyll
0.1 ml stock contains 0.025 mg chlorophyll
1 ml stock contains 0.025/0.1x 1 = 0.25mg/ml (concentration)(1 mk)
NB: vol. (mLs) is variable cause of resuspended pellet + vol pellet was
resuspended in, taken from manual.
Yield
1 ml stock contains 0.025mg
(Vol. total stock you measured Y) contains:-
0.025 x Y = Z mg chloroplast/chlorophyll
Assuming wet weight of plant tissue was 20 g,
Total yield = Z/20 = W mg/g of plant leaves
Tables: Effect of inhibitors on the rate of the Hill reaction:

Table 1: Absorbance readings at 600 nm at each time interval

Table 2: Total change in absorbance (δA) for each time interval
Table 3: Absorbance readings at 600 nm at each time interval
Table 4: Total change in absorbance (δA) for each time interval
Graph 1. Effect of inhibitors on change in absorbance (at 600nm) of DCPIP
with time
Graph 2. Effect of light intensity on δA
Additional questions: Ensure done
Discussion: Discuss any unusual results or deviations
Sources of error/precautions taken
References: In text and bibliography