ACADEMY OF SCIENCES OF MOLDOVA

BOTANICAL GARDEN (INSTITUTE)

UNIVERSITY OF THE ACADEMY OF SCIENCES OF MOLDOVA

CONSERVATION
OF PLANT DIVERSITY
INTERNATIONAL SCIENTIFIC
SYMPOSIUM
3nd edition
22 - 24 May 2014
Chisinau, Republic of Moldova
CONSERVAREA
DIVERSITĂŢII PLANTELOR
SIMPOZION ŞTIINŢIFIC
INTERNAŢIONAL
Ediţia a III-a
22 -24 mai 2014
Chişinău, Republica Moldova

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CZU 58(082)
C 66

Organized by:
Botanical Garden (Institute) of ASM;
University of the Academy of Sciences of Moldova.

In collaboration with:
Ministry of Environment of the Republic of Moldova;
Agency “Moldsilva”;
The Regional Environmental Centre;
Store network “Casa Curata”.

Under the patronage:

Academy of Sciences of Moldova;
Department of Natural and Exact Sciences of the ASM.

Descrierea CIP a Camerei Naţionale a Cărţii

„Conservarea diversității plantelor”, Simp. șt. intern. (3; 2014;
Chișinău). „Conservarea diversității plantelor” = „Conservation of plant
diversity”: Simp. șt. intern., Ed. a 3-a, 22-24 mai 2014 Chișinău, Rep.
Moldova / com. de progr.: Gheorghe Duca [et al.]; − com. org.: Alexandru
Teleuță [et al.]. − Chișinău: S. n., 2014 (Tipogr. AȘM). − 136 p.
Antetit.: Acad. de Științe a Moldovei, Grădina Botanică (Inst.), Univ.
Academiei de Științe a Moldovei. − Tit. paral.: lb rom., rusă. − Bibliogr. la
sfârșitul art. − 200 ex.
ISBN 978-9975-62-370-4.
58(082)
C 66

ISBN 978-9975-62-370-4

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 PROGRAM COMMITTEE:

o Acad. Gheorghe DUCA, president of the Academy of Sciences of

Moldova;
o Dr. Alexandru TELEUŢĂ, director of the Botanical Garden (Institute)
of ASM;
o Acad. Aurelian GULEA, acad. coord. of the Department of Natural and
Exact Sciences of the ASM;
o Gheorghe ŞALARU, Minister of Environment;
o Stefan CHITOROAGĂ, General Manager of Agency “Moldsilva”;
o Acad. Maria DUCA, University of the Academy of Sciences of
Moldova;
o Dr. hab. Natalia ZAIMENKO, director of the National Botanical
Garden “N. Grişko”, Kiev, Ucraina;
o Acad. Alexandru CIUBOTARU, Botanical Garden (Institute) of ASM;
o Correspondent member Vasile ŞALARU, Biology and Pedology
faculty of the Moldova State University.

ORGANIZING COMMITTEE:

o Alexandru TELEUŢĂ, dr., director of the Botanical Garden (Institute)

of ASM;
o Maricica COLŢUN, dr., vicedirector on scientific problems of the
Botanical Garden (Institute) of ASM;
o Eugeniu ALEXANDROV, dr., scientific secretary of the Botanical
Garden (Institute) of ASM;
o Aliona GLIJIN, dr., prof. ass., University of the Academy of Sciences
of Moldova;
o Vasile BUCĂŢEL, dr., head of Dendrology Laboratory;
o Gheorghe POSTOLACHE, dr. hab., head of Geobotanic and forestry
Laboratory;
o Lilia CHISNICEAN, dr., head of Vegetal resources Laboratory;
o Valentina ŢÎMBALÎ, dr., head of Tropical plants Laboratory;
o Nina CIORCHINA, dr., head of Embryology and biotechnology
Laboratory;
o Tatiana SÎRBU, dr., head of Floriculture Laboratory;
o Valentina CANTEMIR, dr., head of Spontaneous flora and herbarium
Laboratory.

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 CUPRINS
I. Structural and functional diversity of vegetal organisms
1. Boz Irina, Necula Radu, Budeanu Oleg, Ghita Georgiana,Gille Elvira. HISTO-
ANATOMICAL RESEARCH REGARDING THE VEGETATIVE ORGANS OF
ORIGANUM VULGARE L........................................................................................9
2. Calalb Tatiana, Onica Elisaveta, Burlescu D. THE CONTENT OF SOME
NATURAL COMPOUNDS IN THE FRUITS OF NEW FORMS OF BLACK
CHOKEBERRY AND SEA-BUCKTHORN, OBTAINED IN BOTANICAL
GARDEN OF ACADEMY OF SCIENCE OF MOLDOVA ..................................10
3. Codreanu V. DETERMINATION OF DROUGHT RESISTANCE OF GRAPEVINE ON
THE BASE OF MORPHOBIOLOGICAL CHARACTERS OF LEAF BLADE.......12
4. Elenciuc Daniela, Bulimag Valentina, Zosim Liliana, Efremova Nadejda, Batîr
Ludmila. THE MODIFICATION OF CONTENT OF SOME ANTIOXIDANTS IN
SPIRULINA PLATENSIS BIOMASS.......................................................................13
5. Găucă Constantin, Popa Lorena-Diana. GENETIC VARIABILITY OF THE
CONTENT OF THE CANNABINOID IN THE IMPROVEMENT PROCESS OF
MONOECIOUS HEMP (CANNABIS SATIVA L.) VAR. MONOICA.....................15
6. Glijin Aliona, Acciu Adriana, Gîscă I. MOLECULAR ANALYSIS OF OROBANCHE
CUMANA WALLR. FROM DIFFERENT GEOGRAPHICAL REGIONS............16
7. Jelev Natalia, Ignatova Zoia, Cuzneţova Irina, Moraru Constantin. SELECTING
OF MUTANT FORMS OF WINTER WHEAT WITH GOOD QUALITIES
OF BREADMAKING QUALITY TESTED IN EXTREM DROUGHT AND
RAINFALL CONDITIONS.....................................................................................17
8. Lisnic S., Toma S., Lemanova Natalia, Coretscaia Iulia. NITRATE REDUCTASE
AND PEROXIDASE ACTIVITY IN SUGAR BEET LEAVES UNDER
APPLICATION OF TRACE ELEMENTS AND TEMPORAL WATER STRESS..19
9. Lozinschii Mariana, Ciorchina Nina. GROWTH REGULATORS INFLUENCE
ON SOME VARIETIES OF BLACKBERRY CULTIVARS DURING THE
MICRO-CLONING PROCESS.........................................................................20
10. Marinescu M., Kolesnikova L. APPLE FRUIT CALLUS: CELLULAR AND
SUBCELLULAR ORGANIZATION.......................................................................22
11. Martea Rodica, Mutu Ana, Clapco Steliana, Budeanu Oleg, Duca Maria.
EVALUATION OF GENETIC DIVERSITY OF ORIGANUM GENUSSPECIES....23
12. Motelica L., Stegărescu V. THE DEGREE OF ACCUMULATION OF THE
RADIONUCLIDE Cs-137 IN SPONTANEOUS VEGETATION.............................25
13. Mutu Ana, Budeanu O., Martea Rodica, Clapco Steliana, Gille Elvira, Port Angela,
Duca Maria. RAPD MOLECULAR MARKER STUDY OF THE INTRASPECIFIC
VARIABILITY OF ORIGANUM VULGARE SUBSP. VULGARE NATURALLY
OCCURRING IN MOLDOVA.................................................................................26
14. Neagu Elena. ANALYSIS OF GENETIC DIVERSITY IN MOLDAVIAN POTATO
CULTIVARS BY USING RAPD MARKERS..........................................................27
15. Negulici Eduard Marius. THE INFLUENCE OF CLIMATE CHANGES ON
PHENOLOGY TO SOME VARIETIES OF ROSES FROM BOTANICAL GARDEN
“DIMITRIE BRANDZA”.......................................................................................29
16. Onica Elisaveta. QUANTITATIVE ANATOMY OF LEAF EPIDERMIS AT
INTERGENERIC TETRAPLOID HYBRIDS QUINCE X APPLE (CYDONIA X
MALUS)...................................................................................................................30

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variability of the expression. Consistent with this idea, the proliferation in
vitro, under nutritional stress, the presence of exogenous hormones (auxins and
cytokinins) and other factors exploit the same multiply mechanism that was
established in vivo, during the evolution of hypertrophy and hyperthely.
Bibliography
1. Colesnicova L.S., Matienco B.T. Investigation of particularities of binucleation in
pericarp cells of old apple cultivars. Rev. of AS of RM, Biol.and chem.., 1994, 1: 30-32
2. Marinescu M., Matienco B. Cytological analysis of tomato fruit callus culture. Proc.
VIII Intern. Congr. Plant Cell Culture. Firenze, 12-17 June, 1994: 266.
3. Matienco B. Comparative anatomy and ultrastructure of Cucurbita fruits. Kishinev:
Cartea Moldoveneasca, 1969: 407 p.
4. Matienco B., Maximova E., Brezeanu A., Marinescu M. Visualization of intracellular
traffic of phenolic compounds during its biosynthesis and translocation in grape berry
callus. Proc. 13th Nat. Electron Microsc. Congr. Ankara, 1-4 Sept., 1997: 167-173.
5. Rumyantseva N.I., Valyeva A.I. et al. Peculiarities of lignification of cell walls of
buckwheat calli with different morphogenic ability. Tsitologiya (in Russian), 1998, 40: 835-843.

EVALUATION OF GENETIC DIVERSITY OF ORIGANUM

GENUS SPECIES
Martea Rodica, Mutu Ana, Clapco Steliana, Budeanu Oleg,
Duca Maria
University of the Academy of Sciences of Moldova

Introduction
Origanum belonging to the Lamiaceae family is one the most wide-spread
genus in the spontaneous flora of many landscapes. It has a precious natural
resource, as they provide raw material for pharmaceutical industry, modern and
traditional forms of medicine and generate employment and income in addition to
conservation of biodiversity [1]. The genus includes 43 species of perennial and
annual plants, which grow on rocky slopes, at a wide range of altitudes [1, 2, 3].
The aerial part of the plant is often used directly while the extracted essential oils
exhibit antimicrobial properties, cytotoxic and antioxidant activities [5].
Taxonomic studies based on morphological characters [3] and on the results
of biochemical analysis of the essential oils and their constituent compounds [5]
indicate a high degree of variability across species of the Origanum genus.
In light of these results, we considered significant to analyze the genetic
polymorphism in two species of the Origanum genus - Origanum vulgare L. and
Origanum laevigatum Boiss., collected from a region of spontaneous flora in
Moldova (Orheiul-Vechi).
Material and methods
The plant material was used in the form of dry leaves. Total genomic DNA was
isolated from fully developed leaves using the CTAB extraction protocol [4], by

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gradually increasing the liquid nitrogen concentration. A total of 8 single decamer
random oligonucleotide primers of arbitrary sequence (OPA2, OPA9, OPA19, OPB01,
OPG05, OPK17 şi Oligo391) were tested for PCR amplification. DNA amplification
according to a basic PCR protocol was performed in a total volume of 15 μL,
containing 50 ng of template DNA, 0,4 - 0,6 μM of single primer, 1 U Taq DNA
polymerase 200 μM of each dNTP, 2,5 mM MgCl2 [6].
Results and discussions
The RAPD-PCR analysis of the Origanum sp. genotypes indicated a high
heterogeneity of the results by genotype and tested primers. Based on the
electrophoretic spectrum, 82 amplification products were identified with an
average of 11,71 bands per primer. The number of amplified fragments varied in
the range from 0 to 12 depending on the primer used.
The comparative analysis of molecular polymorphism across the named
species revealed that the RAPD-PCR generated 47 bands for Origanum vulgare
L. and 35 for Origanum laevigatum Boiss. 23 of them are polymorphic and 35 –
monomorphic fragments, 22 for O. vulgare L., respectively 13 fragments for O.
laevigatum Boiss. The Oligo391 decamer presented six bands for both species, all
monomorphic.
The obtained results allowed concluding that the most informative primers
are: for O. vulgare L. - UBC250 and for O. laevigatum Boiss. - OPK17 showing a
maximum number of fragments (12, respectively 9 bands). OPG5 (O. vulgare L.)
and UBC250 (O. laevigatum Boiss.) decamers did not generate any amplicons. The
most specific bands were revealed in the case of OPA02 (O. laevigatum Boiss. - 4)
and UBC250 (O. vulgare L. - 12) decamers.
Conclusions
RAPD-PCR analysis has established a high heterogeneity between Origanum
vulgare L. and Origanum laevigatum Boiss. Specific particularities of each species
depend by tested primers. The most primers showed a high degree of molecular
polimorfism. The most informative primers are UBC250 for O. vulgare L. and
OPK17 for O. laevigatum Boiss.).
These studies are the part of Moldova-Romanian project “Genetic intraspecific
polymorfysm analysis for the elaboration of molecular markers of some medicinal
and aromatic plants chemotypes”.
Bibliogrphy
1. Cantino, P.D., Harley, R.M., Wagstaff, S.J. Genera of Labiatae: Status and
classification. In:Harley, R. M., Reynolds, T. (eds.) Advances in Labiate Science. Royal
Botanic Gardens Kew, Richmond, Surrey, UK, 1992.
2. Danin, A., Künne, I. Origanum jordanicum (Labiatae), a new species from Jordan,
and notes on other species of sect. Campanulaticalyx, Willdenowia, 1996, 25: 601-611.
3. Ietswaart, J.H. A taxonomic revision of the genus Origanum (Labiatae). Leiden
Botanical Series 4, Leiden University Press, The Hague, 1980.
4. Murray, M.G., Thompson, W.F. Rapid isolation of high molecular weight plant
DNA, Nucleic Acids Res. 1980, 8,(19):4321-4325.

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 5. Padulosi, S. Oregano. Promoting the conservation and use of underutilized and
neglected crops. Proceedings of the IPGRI International Workshop on Oregano, 8-12 May
1996, CIHEAM, Valenzano (Bari), Italy. Institute, ISBN 92-9043-317-5.
6. Williams, J.G.K., Kubelik, A.R., Livak, K.J., et al. DNA polymorphisms amplified
by arbitrary primers are useful as genetic markers, Nucl. Acids Res, 1990, vol. 18, nr. 22.
p. 6531-6535.

THE DEGREE OF ACCUMULATION OF THE RADIONUCLIDE

Cs-137 IN SPONTANEOUS VEGETATION
Motelica L., Stegărescu V.
Institute of Ecology and Geography of ASM
Introduction
Natural and artificial radioactive elements have become vital component
parts of environmental pollution. The main source of soil pollution with artificial
radionuclides and with nuclear decay products has become the global rainfall as a
result of nuclear experiments and the impact of Chernobyl Atomic Power Station.
In the natural ecosystems and those anthropogenic ones some radionuclides (Cs-
137,Sr-90) are taken over very well especially by the plants that are mentioned in
the special literature as bioindicators of environmental radioactivity [1]. For the
evaluation of radionuclide transfer processes from the soil in the plant there are
used different indices, one of them being the coefficient of accumulation (Kac.) [2].
Material and methods
As an object of study served the medicinal plants-stinging nettle (Urtica dioca
L.) and celandine (Chelidonium majus L.) in Landscape Reserve (LR) Holoşnita
and liquorice milkvetch (Astragalus glycyphyllos L.) and moss (Hylocomium
splendens) in LR Cosauti, with reduced accumulation of radionuclides from soil
[1]. In order to determine radiospectrometrically the content of the radionuclides
Cs-137 [3], there were taken samples of ground and vegetation. The accumulation/
transfer degree of the radionuclide was calculated according to the formula:
Kac. = Cpl./CSol, where Cpl. – stands for the radionuclide concentration in the plant
(Bq/kg), and CSol – radionuclide concentration in the soil (Bq/kg) [2].
Results and discussions
Radiospectrometrical analysis of soil samples revealed for the LR Cosăuţi
a quantity of 13.37 Bq/kg (leached chernozem soil) and for the LR Holoşnita –
30.52 Bq/kg (uneroded leached chernozem soil). The highest concentration of the
radionuclide Cs-137 (16.0 Bq/kg) was observed in moss (Hylocomium splendens)
in the LR Cosăuţi compared to 3.0 Bq/kg in wild licorice. In the plants of Urtica
dioca L. and Chelidonium majus L. in the LR Holoşnita the concentration of Cs
137 constituted 6.0 Bq/kg. The calculation of storage/transfer coefficient of Cs-
137 from the soil of protected areas into the plants revealed the following: for
Chelidonium majus L. and Urtica dioca L. – 0.19, Astragalus glycyphyllos L.
– 1.22 and Hylocomium splendens – 1.19. According to the Basic Standards of
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 Bun de tipar 14.05.2014
Format 60x90/16
Coli de tipar 8,5
Comanda nr. 15
Tiraj 200 ex.

Tipografia Academiei de Științe a Moldovei

mun. Chișinău, str. Petru Movilă, 8

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