Professional Documents
Culture Documents
PasolliE_2018
Obregon-TitoAJ_2015
RampelliS_2015
SmitsSA_2017
BritoIL_2016
LiuW_2016
dataset
WenC_2017
HeQ_2017
ChengpingW_2017
LiJ_2017
QinJ_2012
QinN_2014
YuJ_2015
storage
addition of 97% ethanol 10min-5hrs after collection, stored at -23°C till shipping to US in dry ice
on ice up to 4 days, then kept frozen
soaked in 97% ethanol for 24-36hrs, after which ethanol was poured out and sample was transfered in new container with sili
after retrieval from the individual, frozen in liquid nitrogen and kept in dry ice
RNALater (QIAGEN) and frozen at -80°C within 30 min of collection
not specified
storage
frozen -80°C after transfer from hospital to lab
immediately frozen -80°C
frozen -80°C after transfer from hospital to lab
immediately frozen -20°C
immediately frozen -20°C
frozen -80°C after transfer from hospital to lab
frozen in home freezer and then -20°C
horts
extraction
PowerSoil DNA Isolation Kit (MoBio) after pre-heating to 65°C+95°C for 10 min each
PowerSoil DNA Isolation Kit (MoBio) after pre-heating to 60°C+90°C for 10 min each
QIAamp DNA Stool Mini Kit (QIAGEN)
phenol-chloroform extraction with mechanical lysis
PowerSoil DNA Isolation Kit (MoBio) after proteinase K step at 55°C for 20 min
QIAampDNA Stool MiniKit
extraction
phenol/trichloromethane DNA extraction method
alkaline Polyvinylpolypyrrolidone DNA extraction method
phenol/trichloromethane DNA extraction method
TIANGEN kit
alkaline Polyvinylpolypyrrolidone DNA extraction method
phenol trichloromethane DNA extraction
Qiagen QIAamp DNA Stool Mini Kit
library and sequencing
NexteraXT + HiSeq2500
NexteraXT + Illumina
TruSeq DNA PCR-Free Sample prep Kits + Illumina GAIIx
TruSeq DNA PCR-Free Sample prep Kits + Illumina HiSeq 4000
Illumina HiSeq 2000
TruSeq DNA Sample prep v2 + Illumina HiSeq2500