ODYSSEY Fc

IMAGING SYSTEM

One System, Two Detection Methods

Infrared Fluorescence and Chemiluminescence

Quantitative, Two-Color Western Blots

Chemiluminescence Detection

Wide Linear Dynamic Range

No Film or Darkroom

One-Button Image Acquisition

 ODYSSEY Fc
®
The Only System that Offers
IMAGING SYSTEM Superior Infrared and
Chemiluminescence Detection
The Odyssey Fc System offers unique advantages for both infra-
red fluorescent and chemiluminescent Western blot detection.
The Odyssey Fc Imager is the newest member of the Odyssey
Imaging System family of imagers, which continue to set new
standards for quantitative Western blot analysis. The Odyssey
Fc System allows your lab to combine the proven advantages
of quantitative infrared fluorescence detection with simple,
streamlined, qualitative chemiluminescence detection on one
instrument.

Introducing FieldBrite™ XT Technology

Set aside traditional thinking about CCD detectors: the Odyssey
Fc System introduces new laser-based detection technology
that acquires images with uniform field illumination and without
saturated pixels on the first attempt. With the Odyssey Fc System
there is no need for software corrections such as field flattening
and you don’t have to change filters, adjust the focus, or super-
cool the camera.

Quantitative, Two-Color Western Blots High Sensitivity and Wide Dynamic Range

Chemiluminescence Detection
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Wide Linear Dynamic Range

No Film or Darkroom

Strong and weak bands are accurately detected in a single image without
One-Button Image Acquisition signal saturation. Serial dilutions of purified transferrin (10 ng to 1.2 pg) were
detected with rabbit anti-transferrin and IRDye® 800CW secondary antibody.
Acquisition time with the Odyssey Fc Imager was 2 minutes.
Unique to the Odyssey Fc Imager is a one-button image acquisi-
tion system, featuring new FieldBrite XT technology, that quickly
acquires infrared fluorescent and/or chemiluminescent images
uniformly over the widest reported system dynamic range in the
industry, with minimal user adjustments.
strong and weak bands on the same blot without worrying about
fuzziness, blowouts, or hidden bands. You can detect two targets
simultaneously on the same membrane to increase the accuracy
of quantification and comparison using multiplex imaging on the
Odyssey System.

The Odyssey Fc System features two-channel infrared fluores- At the 700 and 800 nm infrared wavelengths detected by the
cence detection (700 and 800 nm) plus a third detection channel Odyssey Fc Imager, both autofluorescence and light scatter
for chemiluminescence. This provides the two-channel, multiplex are dramatically reduced. Emission signals are captured in this
detection that is well known to Odyssey users and eliminates the near-infrared ‘sweet spot,’ resulting in the cleanest background,
need for a darkroom. highest signal-to-noise ratios, and the best detection sensitivity
available with a fluorescent system.
Cy3 Cy5 LI-COR LI-COR
(550 nm) (649 nm) IRDye® 680LT IRDye® 800CW
(680 nm) (780 nm)
The Fc Chemiluminescence Advantage
First, you eliminate film and the time spent in the darkroom doing
Visible Fluorophores Infrared Fluorophores multiple exposures in order to “get the best image.” The Odyssey
400 600 800 1000
Fc System offers flexibility rather than guesswork for chemilu-
Wavelength (nm)
minescence detection. You also avoid the accompanying mess
and hazardous waste of film development. Plus, the Odyssey Fc
The Infrared Fluorescence Advantage System, using FieldBrite XT technology, delivers uniform imaging
Infrared fluorescence detection provides quantitative analysis across the field of view and consistent image quality without the
and wide linear dynamic range that is not available with tradi- blowouts or saturation that can occur with film or other chemilu-
tional methods. Only the Odyssey family of imagers detects minescence imaging systems.

The Odyssey Fc System is the practi-

cal solution to improve your Western blot
workflow, whether you use infrared fluo-
rescence or chemiluminescence detection.
The Odyssey Fc System adapts to the
demands of your lab and offers:

n One-button image acquisition

n Superior image quality

n Detection method flexibility

n  Powerful analysis software

Why Infrared Fluorescence?
Unique to all Odyssey ® platforms is the use of infrared laser visible fluorescence systems and makes it difficult to detect low-
excitation that out-performs visible fluorescence systems. In the abundance proteins at endogenous levels without saturation of
visible wavelength range used by most fluorescence imagers, stronger bands.
membranes and plastics produce high background due to light
scattering and autofluorescence. This limits the sensitivity of

Membrane Autofluorescence is Dramatically Reduced

3000000
Membrane Fluorescence

2500000

2000000

1500000

1000000

500000

0
532nm 635nm 700nm 800nm
Wavelength

Nitrocellulose and PVDF membranes were imaged with the Odyssey Infrared Imaging System at an
Intensity = 5 for both 700 and 800 nm wavelengths. The same membranes were scanned at a 532 nm
and 635 nm wavelength with a PMT = 500 on a GenePix® 4100A (Molecular Devices). Autofluorescence was
much lower at infrared wavelengths.

The Odyssey Family

The Odyssey family includes the Odyssey Infrared Imaging
System, which continues to set the standard for quantita-
tive Western blot analysis while providing versatility for a
wide variety of membrane, gel, microplate, and in vivo
applications. The Odyssey Sa Imager is designed to handle
Western blots and microplate assays, such as In-Cell Western™
Assays with optional walk-away automation. The new Odyssey
Fc Imager is the only system that offers superior infrared
quantitative fluorescent Western blot imaging and chemilumines-
cence on the same platform.
Quantification with Confidence Wide Dynamic Range for Accurate Quantification

Through the innovative use of infrared fluorescent antibody

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conjugates, the Odyssey® Fc Imager provides a broad, linear

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dynamic range to accurately detect both strong and weak bands
on the same Western blot. In contrast, the dynamic enzymatic
nature of chemiluminescence allows you to capture only a “snap-
shot” of the enzymatic reaction and is highly dependent on
timing and exposure. The unusually wide dynamic range allows precise quantification of both
strong and weak bands, to more accurately reflect protein levels. Serial
dilutions of purified transferrin (10 ng to 1.2 pg) were detected with rabbit
anti-transferrin and IRDye® 800CW secondary antibody. Acquisition time with the
The accuracy and linearity of fluorescence detection on the
Odyssey Fc Imager was 2 minutes. Triplicate blots were performed.
Odyssey Fc System allow you to feel confident about differences
you see in protein levels. And your near-infrared fluorescent blots
can be archived then imaged, and quantified again months later,
if needed.

R2 = 0.9942

Fluorescent signals on the triplicate blots were quantified with Image Studio soft-
ware. Errors bars are shown, but are very small. Results were linear across
the entire range tested (4,000-fold; 3.6 orders of magnitude).

Multiplex Western Detection: More Data, Improved Accuracy

The two infrared fluorescence detection channels of the Odyssey Fc System enable two-color target analysis – an advantage that’s
not available with chemiluminescent or radioactive methods. Two-color Western analysis makes normalization easy and eliminates
error introduced by stripping and reprobing or by comparison of separate blots. Superior image clarity and detail allow you to resolve
co-migrating proteins and make it easier to detect subtle mobility shifts caused by protein modifications such as phosphorylation.

kDa
250
150
100
75

50

37

700 nm Image 800 nm Image Overlaid Images

Detect Two Targets and Monitor Protein Phosphorylation. Lysates (10 μg/well) of A431 cells treated with EGF were separated and transferred to nitrocellulose. The
blot was probed with rabbit anti-ERK1 and mouse anti-phospho-ERK primary antibodies (Santa Cruz Biotechnology) and then detected with goat anti-rabbit IRDye 680
(red) and goat anti-mouse IRDye 800CW (green) secondary antibodies, respectively. The blot was imaged with the Odyssey Fc Imager for 2 minutes in each channel.
Overlapping ERK (red) and phospho-ERK (green) signals are displayed in yellow. This phospho-ERK1 antibody cross-reacts with phospho-EGFR (upper green band).
Chemiluminescence
For qualitative chemiluminescent Western blot detection,
the Odyssey ® Fc System saves you time and eliminates the
guesswork of multiple exposures as well as the expense and
hazardous waste of film processing.

Saturation and blown-out bands are now a thing of the past.

With the Odyssey Fc System’s one-button image acquisition, a
single digital image replaces a stack of time-consuming multiple
exposures.

The wide dynamic range of the Odyssey Fc System allows both

strong and weak chemiluminescent bands to be clearly captured
With the Odyssey Fc System, chemiluminescent imaging is
with only one exposure. This is especially valuable if you wish
streamlined for excellent results with minimal user adjustments
to quantify the chemiluminescent signal within its available linear
and without the need for multiple exposures.
range. LI-COR® FieldBrite™ XT technology compensates for time
and substrate signal variance issues common to chemilumines-
cence methods.

Chemiluminescence: Odyssey Fc Imager and Film

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Odyssey Fc Film

Chemiluminescence detection of purified transferrin, using the Odyssey Fc imager or film exposure. Detection sensitivity is similar. With Odyssey Fc detection,
blowout of stronger bands is reduced and band resolution is improved.
Simply the Best Signal-to-Noise Ratios and Dynamic Range
FieldBrite™ XT technology features a patented filtering system
that delivers low total noise for acquisition times of up to one
hour. Signal-to-noise ratio is optimized as image acquisition
progresses to ensure superior image quality and level of detec-
tion. This advanced laser-based detection platform delivers high
sensitivity without the need to manipulate data by binning, which
can compromise image resolution.
Traditional CCD imagers typically supercool the camera in an
effort to reduce noise and improve image quality. Supercooling
requires additional energy consumption to maintain operational
efficiency and causes longer wait times when powering up the
camera.
Chemiluminescence, a dynamic enzymatic method, delivers
qualitative and semi-quantitative detection across a narrower
linear range. With the Odyssey Fc System, chemiluminescent
imaging is streamlined for excellent results with minimal user
adjustments and no multiple exposures.
The Odyssey Fc System is the ideal solution for researchers who
depend on both detection methods to achieve their research
goals. Now you can combine both methods in a single imager
saving time, lab space, and valuable research dollars.
Laser Module CCD Detector

FieldBrite XT technology reduces total noise without the need

to supercool the camera. This innovative, patented tech-
nology yields very low total noise and can deliver up to 6 logs
of dynamic range for superior sensitivity and imaging quality for
both detection methods.

Wide Dynamic Range

With its wider dynamic range, the Odyssey ® Fc Imager accu-
rately captures both strong and weak signals in the same image.
Delivering superior image quality with laser-based infrared fluo-
rescence is a hallmark of the Odyssey systems. The Odyssey Fc
System continues this tradition of detecting low and high protein
concentrations in a single image without signal saturation.

Two Methods, One System

Infrared fluorescent imaging offers the greatest precision for
quantitative Western blot analysis. With the proven accuracy
and linearity of the Odyssey Fc System, you can be confident of
the differences you see in protein levels.
Imaging Tray
Laser module contains a 685 nm laser and a 785 nm laser. FieldBrite XT
technology ensures uniform illumination of the sample, low coefficient of
variation (%CV), and exceptional reproducibility. Illustration depicts activation
of 685 nm channel.
Powerful, Easy-to-Use Analysis Software
Introducing Image Studio
Image Studio software is the key to fast, easy image acqui-
sition and analysis with the Odyssey® Fc Imager. Push a
button, and the blot is imaged in the detection channels
you choose. Select one- or two-channel infrared fluores-
cence, one-channel chemiluminescence, or, when needed,
combine infrared fluorescence and chemiluminescence.

Intuitive ribbon formats provide easy navigation and image

analysis flexibility that can be fine-tuned to your specific
research needs. This easy-to-learn and easy-to-use inter-
face includes dedicated ribbons for Westerns and MPX™
Westerns, to further streamline your workflow.

With Image Studio, you can lock the image settings of

multiple images for accurate visual comparison, even with
images taken months apart.

Dedicated Western Analysis and MPX™ Western ribbons offer tools for quick placement
of lane and band markers on the image to speed up data analysis.

Use sliders to adjust the appearance of the image

on the screen. Lock the settings across multiple images
for accurate comparison.
Odyssey Fc Applications

IR Fluorescent Western Blot Chemiluminescent Western Blot Protein Gel Documentation

The Odyssey Fc System provides

a flexible solution to a variety of
imaging needs, including:

n Quantitative IR Fluorescent
Western Blots
n Chemiluminescent
Western Blots
DNA Gel Staining using Syto 60 Stain ®
Western Blot Optimization or Antibody n Protein Gel Documentation
Screening Using MPX™ Blotting System
(See next page) n DNA Gel Documentation

Lab Book Report

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Now you can easily create electronic or hard-copy lab reports custom- You can easily design multiple templates and prepare various reports
ized to meet your specific needs. Design customized templates in Image based on your intended audience. If your record-keeping method must
Studio using a drag-and-drop approach to arrange the content. Then, vary to meet different documentation requirements (such as GLP or ISO),
simply select the desired template to format your data for a custom you can quickly re-arrange a report or add information while maintaining
report. your original Lab Book Report.
MPX™ Blotting System
The MPX Blotting System is ideal for multiple-target Western
blot procedures that use PVDF or nitrocellulose membranes
(7 x 8.5 cm). It also allows you to quickly optimize experimental
conditions – blocking buffers, primary antibodies, and/or
secondary antibodies – in one easy experiment.

Low-volume channel ports (<160 μL) conserve antibodies

and reduce costs. Twenty-four channel ports are conveniently
spaced, staggered, and compatible with multi-channel pipettes.
Forty-eight targets can be visualized on a single membrane with
two-color detection. The MPX Blotting System is compatible with
many prep gels.

FEATURES MPX Blotting System and representative Western blot imaged with the
Odyssey® Fc System shows evaluation of multiple primary and secondary
n S
 creen a single sample and multiple targets on the antibody concentrations.
same blot
n Screen up to 48 different targets with two-color detection
n Optimize blocking and antibody dilution parameters
n Conserve antibody and reagents
n Compatible with a variety of well configurations

Western Reagents and Accessories

LI-COR Biosciences IRDye ® infrared conjugates are optimized for a wide variety of applications. Odyssey Fc System
applications include Western blots, MPX Westerns, and gel documentation. For more information about LI-COR Biosciences reagents
and accessories designed to streamline your research, please view our online catalog or download our Products and Applications
Guide at www.licor.com/bio.

n IRDye secondary antibody conjugates n Smart™ Gels

n B
 locking buffers n Western blot detection kits
n M
 embranes n Incubation boxes
n P
 rotein Molecular Weight markers n Accessories

Available online at:

www.licor.com/bio
 System Specifications
Related Products
Image Field Size:
10 cm x 12 cm

CCD Pixel Size:

6.45 microns

Dynamic Range:
22 bit (>6 logs)

Odyssey Infrared Imaging System Odyssey Sa Imaging System

Depth of Field for Best Sample Focus:
www.licor.com/odyssey www.licor.com/odysseysa 6 mm

Patented FieldBrite™ XT Technology:

®
n Odyssey Fc Imaging Trays CV <3% across field
n O
 dyssey Blocking Buffers Laser Lifetime:
n IRDye® Infrared Dyes and Conjugates 20,000 hours of operation
n B
 lot Washer
700 Channel Laser Source:
n Western Incubation Boxes
Solid-state Laser Diode at 685 nm
n MPX Western Kit and Combs
n NucleoCounter® NC-100 for Automated Cell Counting 800 Channel Laser Source:
Solid-state Laser Diode at 785 nm
n Odyssey Pens
n Odyssey Soft Roller, 4 inches Detectors:
Low-noise CCD. Thermoelectrically cooled.

For more information on the Odyssey Fc Imaging System, Acquisition Times:

including applications and protocols, go to: • Fluorescence (700 and 800 nm) channels: 30 s,
www.licor.com/odysseyfc 2 min, 10 min plus variable time feature

• Chemiluminescence channel: 30 s, 2 min,

10 min, 60 min plus variable time feature

Focusing:

Westerns on the Odyssey Systems Automatic

Better Data, Cleaner Planet Operating Conditions:

Imaging with Odyssey Systems does not
require film, excessive water washes, or
hazardous waste associated with film
development.
For more details, visit:
www.licor.com/green
For indoor use only; operating temperature 15-35°C and
dewpoint < 22ºC, non-condensing; maximum operating
temperature may be reduced at elevations above 2000 m.
Power Requirements:
Universal input between 100-127 VAC (4 Amp) and 200-240
VAC (2 Amp); 50-60 Hz. Voltage fluctuations not to exceed
10% of the nominal voltage. Insulation Category II.
Dimensions:
41.4 cm W x 47 cm D x 67.3 cm H (16.3” W x 18.5” D x 26.5” H).
Depth with imaging drawer open is 59.7 cm (23.5”).

Weight:
27 kg (60 lb)

ETL/CETL/CE Approved
Locations WorldWide
U.S.
LI-COR® Biosciences
4647 Superior Street
Lincoln, NE 68504
Phone: 402-467-0700
Phone: 800-645-4267
Fax: 402-467-0819
Email: biosales@licor.com

LI-COR GmbH, Germany

Serving Europe, Africa, and the Middle East

LI-COR Biosciences GmbH

Siemensstraße 25A
D-61352 Bad Homburg
Germany
Phone: +49 (0) 6172 17 17 771
Fax: +49 (0) 6172 17 17 799
Email: gmbh@licor.com

LI-COR Ltd., UK
Serving UK, Ireland, and Scandinavia

LI-COR Biosciences UK Ltd

St. John’s Innovation Centre
Cowley Road
Cambridge
CB4 0WS
United Kingdom
Phone: +44 (0) 1223 422104
Fax: +44 (0) 1223 422105
Email: UK@licor.com

View a complete list of our international distributors at:

www.licor.com/bio/distributors

LI-COR is an ISO 9001 registered company. © 2011 LI-COR Inc.
Specifications subject to change. LI-COR, Odyssey, MPX, Field-
Brite, In Cell Western, and IRDye are trademarks or registered
trademarks of LI-COR, Inc in the United States and other coun-
tries. All other trademarks belong to their respective owners. The
Odyssey Infrared Imager, Odyssey Sa, Odyssey Fc Imager, Field-
Brite technology, and IRDye Infrared Dyes are covered by U.S.
patents, foreign equivalents, and other patents pending.
The LI-COR board of directors would like to take this opportunity
to return thanks to God for His merciful providence in allowing
LI-COR to develop and commercialize products, through the col-
lective effort of dedicated employees, that enable the examination
of the wonders of His works.
“Trust in the LORD with all your heart and do not lean on your
own understanding. In all your ways acknowledge Him, and He
will make your paths straight.”
—Proverbs 3:5,6

980-11759 03/11