Dissection or Scanning Electron Transmission Electron

Compound Confocal Microscope

Stereoscope Microscope (SEM) Microscope (TEM)

Compound microscopes
are light illuminated.
The image seen with A dissection microscope is This microscope uses a laser
TEM is electron
this type of microscope light illuminated. The image light. This light is used SEM use electron illumination. The
illuminated. This gives a 2-
is two dimensional. that appears is three because of the wavelength. image is seen in 3-D. It has high
D view. Thin slices of
This microscope is the dimensional. It is used for Laser light scan across the magnification and high resolution.
specimen are obtained.
Description most commonly used. dissection to get a better look specimen with the aid of The specimen is coated in gold and
The electron beams pass
You can view at the larger specimen. You scanning mirrors. Then image the electrons bounce off to give you
through this. It has high
individual cells, even cannot see individual cells is then placed on a digital and exterior view of the specimen.
magnification and high
living ones. It has high because it has a low computer screen for The pictures are in black and white.
resolution.
magnification. magnification. analyzing.
However, it has a low
resolution.
Costs $150 - $10,000 $100-$1500 $20,000-100,000 more than $50.000 more than $50,000
Source of Radiation
visible light visible light laser light electrons electrons
for Image Formation
Medium air air air vacuum vacuum
Thin films of collodion or
glass slides with dyed Mounted on aluminum stubs and
Specimen mounting glass slides none other supporting material
samples are coated in gold
on copper grids
glass lenses with dichromatic one electrostatic lens with a few one electrostatic lens and a
Nature of Lenses glass glass
mirrors electromagnetic lenses few electromagnetic lenses
Electrical i.e. current of the
digital computer motorized objective lens coil is
Focusing mechanical mechanical electrical
focusing mechanism
changed.
Electrical i.e. changing
Magnification
changing objectives usually 1 objective digitally enhanced electrical current of the projector
Adjustments
lens coil
Major Means of
light scattering or light laser light with dicromatic
Providing specimen Light Absorption electron scattering Electron scattering
reflection mirror concentrated at pinhole
Contrast

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