Isolation of carvone by preparative gas chromatography

Two anantiomers
+ (carvone) - (carvone)
+cavone isolated from caraway seed oil and – carvone from
spearmint oil, by using Gas chromatography.
+ carvone and – carvone anantiomers have odors distinctly
different from each other.
With the exception of of their optical rotation, the physical
properties of anantiomers are identical.

Caraway seed oil:

contains lager amount of limonene and + carvone.
Spearmint oil:
Contains smaller amount of limonene and -carvone.

Procedure:
Inject 50-mL of caraway-seeds or spearmint oil onto the gas
chromatography column.
Just before a component of oil (limonene or carvone) elutes
from column, install a gas collection tube at the exit port.
Should connect the gas collection tube just before limonene or
carvone elutes from column and remove the tube as soon as all
the component has been collected, but before any other
compound begins to elute from the column.

This procedure is relatively easy for collection carvone

component of both oils. Because of the presence of several
terpenes in spearmint oil, create difficulty to isolate pure
sample of limonene from spearmint oil.
Should must try to collect only the pure component.

After collecting the sample, detect the sample through recorder

which shows peaks. through anaylsis of peaks, isolate the pure
carvon.