SKA3023 INSTRUMENTATION ANALYTICAL CHEMISTRY

EXERCISE 1
SPECTROSCOPY/SPECTROMETRY

1. Calculate the frequency in hertz (Hz) of

a) An X-ray beam with a wavelength of 2.65 Å.
b) The line at 694.3 nm produced by a ruby laser.
c) An infrared absorption peak at 19.6 μm.

2. Calculate the wavelength in centimetres of

a) An airport tower transmitting at 118.6 MHz.
b) An NMR signal at 105 MHz.

3. Calculate then energy of

a) A 5.30 Å X-ray photon.
b) A 530 nm photon of visible radiation.

4. Calculate the wavelength in cm and the energy in joules associated with a signal at
220 MHz.

5. Briefly describe the UV, Visible and IR regions.

6. In principle, there are three types of internal energy are quantized. Discuss how these
energies quantized at discrete levels.

7. Compare and discuss the relative energy levels of three transition process (rotational,
vibrational, electronic).

8. Distinguish three types of spectra (lines, bands. continuum).

9. What is the relationship between Absorbance (A) and transmittance (T).

10. Several spectrometers have scales that read either in absorbance or in percent
transmittance.

a) What would be the absorbance reading at percent transmittance of 25?

b) What would be the transmittance reading at 0.53 absorbance?

SKA3023 Instrumentation Analytical Chemistry 2021 Exercise 1 Azlan Kamari

11. A coloured solution is placed within a spectrometer. At 465 nm the solution shows an
absorbance of 0.79. Calculate the percentage of light that is being absorbed.

12. A solution within a spectrometer shows an absorbance of 0.67 at a wavelength of 560

nm. What is the percentage of radiation that is transmitted?

13. A 0.1 M solution within a 1 cm cell was placed in a spectrometer. The solution shows
an absorbance of 0.95. Calculate the molar absorptivity (ɛ) for this compound.

14. A compound with a molar absorptivity of 3578 L mol -1 cm-1 at 650 nm exhibits an
absorbance of 0.78 when placed in a 1 cm cell in a spectrometer. Calculate the
concentration of the compound.

SKA3023 Instrumentation Analytical Chemistry 2021 Exercise 1 Azlan Kamari