1

The broadening of sample band along the axial direction of column, resulted
from concentration gradient, is known as

a.
Phase diffusion
b.
Mass transfer under non-equilibrium
c.
Eddy Diffusion
d.
Longitudinal Diffusion
e.
Chromatographic diffusion
QUESTION 2
Longitudinal diffusion can be minimized by

a.
Using longer columns
b.
Using low mobile phase velocity
c.
Using column of large diameter
d.
Using columns in coiled form
e.
Using short columns

Plate theory describes the ______ effect of matrix components on sample

analyte during chromatographic elution.

a.
Neither retarding nor reinforcing effect
b.
Retarding effect
c.
None of these
d.
Either retarding or reinforcing effect
2

e.
Reinforcing effect

The value of HETP for a sample component is maximum, if

a.
It has high elution volume
b.
Its retention time is high
c.
Its peak is sharp
d.
VR is high
e.
Its peak area is very high
The sample component that has only smaller height for its theoretical plates in
a given chromatographic column, will show

a.
Only a few binding functional groups on stationary phase
b.
Greater interaction to Mobile Phase
c.
Very small number of theoretical plates in the given column
d.
Very fast flow rate along with mobile phase
e.
Greater interaction to Stationary Phase

Straight line for factor A in the van Deemter Plot indicates that HETP

a.
Increases with increasing velocity of mobile phase
3

b.
Is not effected by eddy diffusion
c.
Decreases with increasing velocity of mobile phase
d.
Remains unaffected by increasing velocity of mobile phase
e.
Have fix contribution by eddy diffusion which is independent of mobile phase velocity

The efficiency of chromatographic column is ________ with increasing factor

of eddy diffusion

a.
None
b.
Slightly increased
c.
Remains unaffected
d.
Decreased
e.
Increased

If stationary phase particle size distribution is large, chromatographic peak

becomes broader due to high value of

a.
Mobile phase flow rate
b.
Eddy Diffusion
4

c.
Longitudinal Diffusion
d.
Mass transfer under non-equilibrium
e.
Number of Theoretical plates

The increase in length of column results in high resolution efficiency due to

a.
Greater eddy diffusion
b.
Greater longitudinal diffusion
c.
Increased number of theoretical plates
d.
Reduced number of functional groups
e.
Slowing down the mobile phase flow rate

At each equilibrium stage, in the column, the component whose greater

number of molecules are in the mobile phase, will have

a.
High number of theoretical plates
b.
Sharp Peak
c.
Low theoretical plate height
5

d.
High retention time
e.
Low retention time

In case of capillary gel electrophoresis, separation depends on

a.
Nature of charge
b.
Charge density
6

c.
All of these
d.
Molecular size

In capillary electrophoresis, negatively charged ions move towards cathode

under the effect of

a.
Concentration gradient
b.
Electrophoretic mobility
c.
Electro osmotic flow
d.
pH gradient

In capillary electrophoresis process, during an electro osmotic flow (EOF)

from anode to cathode, the ions will have retention time in the following order

a.
A2+>A+>A>A->A2-
b.
A2->A->A>A+>A2+
7

c.
A>A2+>A2->A+>A-
d.
A->A2->A>A2+>A+

In capillary electrophoresis, the cations move towards cathode under the

effect of

a.
Electrophoretic mobility
b.
EOF
c.
Neither electrophoretic mobility nor EOF
d.
Both electrophoretic mobility and EOF

Longitudinal diffusion in capillary electrophoresis results in

a.
Fast speed of analysis
b.
Band broadening
c.
Narrow peaks in electropherogram
8

d.
Faster separation

The process of electrophoretic separation begins with ________ as initiation

step

a.
Application of voltage
b.
Equilibration of electrophoretic medium
c.
Generation of sample wells
d.
Sample application

The electrophoretic mobility of sample components depends upon many

factors, except

a.
Size and shape of molecule
b.
Concentration of molecule
c.
9

Net charge on molecule

d.
Strength of electrical field

Which of the followings, is the major advantage of capillary electrophoresis

over other electrophoretic techniques

a.
Improves electro-osmotic flow
b.
Improves electrophoretic mobility
c.
Removes Joule heating effect
d.
Improves the volatility of sample components

The mobile phase in electrophoretic separations, is mostly a

a.
Buffer of variable ionic strength
b.
Liquid of low polarity
c.
Gas
10

d.
Organic solvent

Which one of the followings, is not the basic function of buffer, in

electrophoresis process

a.
Carry applied electric current
b.
Control extent of solute ionization
c.
Maintain pH of eletrophoretic experiment
d.
Maintain temperature of eletrophoretic experiment
11

High speed of analysis of HPLC corresponds to

a.
Very fine particle size of stationary phase
b.
Pressurized flow of mobile phase solvent
c.
Using microporous stationary phase particles
d.
Using porous layer beads as stationary phase

One, which is not requirement of mobile phase of HPLC

a.
Free from dissolved gases
b.
Must give strong detector signal
c.
Non-reactive towards sample components
d.
Free from any particulate matter

Quantitative analysis by HPLC is performed by analyzing

a.
Peak intensity
12

b.
Peak retention time
c.
Peak Area
d.
Peak retention volume

During injection of sample solution into HPLC instrument, when knob of

injection port is at “loading position” then sample solution is

a.
Going to wastage
b.
Moving toward guard column
c.
Being filled in the sample loop
d.
Being carried by mobile phase to the column

The presence of different types of sample components can be monitored by

HPLC analysis in terms of

a.
Different percent of HPLC peak areas
b.
Different peak areas of HPLC peaks
c.
13

Different intensities of HPLC peaks

d.
Different retention times of HPLC peaks

The pump that involves the application of pressure of a gas to generate a

constant, uniform flow rate of mobile phase in HPLC, is

a.
Reciprocating Pump
b.
Pneumatic Pump
c.
Centrifugal Pump
d.
Syringe Pump

The major function of guard column is

a.
To boost up the function of detector
b.
Increase flow rate of mobile phase
c.
To capture target analyte and separate it from rest of sample components
d.
Increase lifetime of analytical column
14

Only a few peaks in HPLC chromatogram represent

a.
High concentration of sample components
b.
Pure sample
c.
Low concentration of sample components
d.
Highly impure sample

Photometric detector is not suitable for compounds having ______ in their

chemical structure

a.
All sigma bonds
b.
π bond
c.
Chromophoric group
d.
Conjugated double bonds
15

Major limitation of Differential Refractometer is that

a.
Can’t be used with isocratic elution
b.
Can’t be used at very high pressure outputs
c.
Can’t be used with gradient elution
d.
Can’t be used with fluorescent compounds
 Started on Saturday, 30 May 2020, 8:30 PM
State Finished
Completed on Saturday, 30 May 2020, 8:44 PM
Time taken 14 mins 13 secs
Grade 5 out of 10 (50%)
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According to w hich model of plate theory, there is perfect mixing of solute

betw een stationary and mobile phases throughout the column

Select one:
a. Discrete flow model
b. Distinct flow model
c. Continuous flow model
d. I solated flow model
e. Remote flow model
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The correct answ er is: Continuous flow model

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According to plate theory, the presence of solutes other than analyte, may lead
to_______on analyte molecules.

Select one:
a. Either retarding or reinforcing effect
b. None of these
c. Neither retarding nor reinforcing effect
d. Reinforcing effect
e. Retarding effect
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The correct answ er is: Neither retarding nor reinforcing effect

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Eddy Diffusion can be minimized by

Select one:
a. Using high flow rate mobile phase
b. Using v ery fine particle size of stationary phase
c. Using columns of greater length
d. Using large diameter columns
e. Using v ery low rates of mobile phase
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The correct answ er is: Using v ery fine particle size of stationary phase
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According to v an Deemter Plot, multiple pathw ay diffusion__________ w ith

increasing flow rate of mobile phase.

Select one:
a. I ncreases to small extent
b. Decreases to small extent
c. I ncreases greatly
d. Decreases greatly
e. Remains unaffected
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The correct answ er is: Remains unaffected

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The component of sample mixture, for w hich height of theoretical plate has
minimum v alue, w ill hav e

Select one:
a. Greater interaction to Stationary Phase
b. Greater interaction to Mobile Phase
c. Greater number of binding functional groups on stationary phase
d. Both (a) and (c)
e. Both (b) and (c)
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The correct answ er is: Both (a) and (c)

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The v alue of term A becomes equal to zero, w hen operating on

Select one:
a. I on exchange column
b. Electro focusing column
c. GC packed column
d. GC capillary column
e. HPLC column
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The correct answ er is: GC capillary column

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Van Deemter, a Dutch chemical Engineer, is regarded as pioneer of ________theory

of elution through a chromatographic column

Select one:
a. Plate Theory
b. Time theory
c. Theoretical Plate theory
d. Coat theory
e. Rate Theory
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The correct answ er is: Rate Theory

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At each equilibrium stage, in the column, the component w hose greater number of
molecules are in the stationary phase, w ill hav e

Select one:
a. High theoretical plate height
b. Low theoretical plate v alue
c. High retention time
d. Broad Peak
e. Low retention time
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The correct answ er is: High retention time

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The straight line for term A in the v an Deemter plot indicates that

Select one:
a. Peak broadening is unaffected by Eddy Diffusion
b. Peak broadening is unaffected by the mobile phase v elocity
c. Eddy Diffusion is unaffected by flow v elocity of mobile phase
d. Height of theoretical is unaffected by Eddy Diffusion
e. Multiple pathw ay diffusion does not influence the mobile phase v elocity
Feedback

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The correct answ er is: Eddy Diffusion is unaffected by flow v elocity of mobile phase

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Question text

I f stationary phase particle size distribution is large, chromatographic peak

becomes broader due to high v alue of

Select one:
a. Longitudinal Diffusion
b. Number of Theoretical plates
c. Mobile phase flow rate
d. Eddy Diffusion
e. Mass transfer under non-equilibrium
Feedback

Your answ er is incorrect.

The correct answ er is: Eddy Diffusion
Sample comprising of thermally labile compounds is mostly introduced into GC
chromatograph by
Select one:
a. Direct Vaporization Injection
b. Splitless Injection
c. Cold on-column injection
d. Microliter pipette
e. Split injection
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The correct answer is: Cold on-column injection

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Which one of the followings, is not the basic requirement of the liquid stationary
phase used in GC
Select one:
a. It should have low vapour pressure
b. It should be heat stable
c. It should be chemically inert
d. It should have high decomposition temperature
e. It should be volatile
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The correct answer is: It should be volatile

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Which one of the followings, is not the basic function of buffer, in electrophoresis
process
Select one:
a. Maintain temperature of eletrophoretic experiment
b. Carry applied electric current
c. Maintain pH of eletrophoretic experiment
d. Carry the electrophoretic mobility
e. Control extent of solute ionization
Feedback

The correct answer is: Maintain temperature of eletrophoretic experiment

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The increase in length of column results in high resolution efficiency due to

Select one:
a. Greater longitudinal diffusion
b. Greater eddy diffusion
c. Increased number of theoretical plates
d. Reduced number of functional groups
e. Slowing down the mobile phase flow rate
Feedback
The correct answer is: Increased number of theoretical plates

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The pump that involves the application of pressure of a gas to generate a constant,
uniform flow rate of mobile phase in HPLC, is
Select one:
a. Hydraulic Pump
b. Reciprocating Pump
c. Pneumatic Pump
d. Centrifugal Pump
e. Syringe Pump
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The correct answer is: Pneumatic Pump

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Which one of the followings, is not the basic requirement of gas selected for mobile
phase in GC
Select one:
a. Should be capable of being detected by detector
b. Should be chemically inert
c. Should be capable of separating sample components
d. Should be economically cheaper
e. Should be easily available
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The correct answer is: Should be capable of being detected by detector

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In capillary electrophoresis process, during an electro osmotic flow (EOF) from

anode to cathode, the ions will have retention time in the following order
Select one:
a. A2->A->A>A+>A2+
b. A->A2->A>A2+>A+
c. A2+>A+>A>A->A2-
d. A>A2->A2+>A->A+
e. A>A2+>A2->A+>A-
Feedback

The correct answer is: A2->A->A>A+>A2+

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The value of retention factor (k) will be high, if value of

Select one:
a. to is high
b. tR is small
c. tR is small, while to is high
d. Both tR and to are small
e. to is small, while tR is high
Feedback

The correct answer is: to is small, while tR is high

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Which of the following factors doesn’t affect the separation process by

chromatographic techniques?
Select one:
a. Temperature of Lab.
b. Intensity of light
c. Direction of Lab.
d. Extent of Aeration
e. Wind speed
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The correct answer is: Direction of Lab.

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Question text

Which of the followings, is not the general requirement of pumping system of HPLC
Select one:
a. Pressure outputs of 500-6000Psi
b. Pulse free solvent flow
c. Small hold up volume
d. Purging the mobile phase to remove dissolved air
e. Flow rate of 3-100mL/min
Feedback

The correct answer is: Purging the mobile phase to remove dissolved air

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The area of each of two separated peaks can be accurately measured, if

resolution (RS) factor has a value of
Select one:
a. ≥ 1.5
b. 1.0 – 1.2
c. 1.2 – 1.5
d. 0.5 – 1.0
e. 0.1 – 0.5
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The correct answer is: ≥ 1.5

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The broadening of sample band along the axial direction of column, resulted from
concentration gradient, is known as
Select one:
a. Longitudinal Diffusion
b. Mass transfer under non-equilibrium
c. Eddy Diffusion
d. Phase diffusion
e. Chromatographic diffusion
Feedback

The correct answer is: Longitudinal Diffusion

Question 13
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If two components have same retention times, one with lesser number of theoretical
plates will have
Select one:
a. All of these
b. Narrower peak
c. Smaller peak width
d. Larger peak width
e. Sharp peak
Feedback
The correct answer is: Larger peak width

Question 14
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Which of the followings, is the major advantage of capillary electrophoresis over

other electrophoretic techniques
Select one:
a. Improves electro-osmotic flow
b. Improves the volatility of sample components
c. Improves the distillation prcess
d. Improves electrophoretic mobility
e. Removes Joule heating effect
Feedback

The correct answer is: Removes Joule heating effect

Question 15
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The presence of different types of sample components can be monitored by HPLC

analysis in terms of
Select one:
a. Different peak areas of HPLC peaks
b. Different retention times of HPLC peaks
c. Different shapes of HPLC peaks
d. Different intensities of HPLC peaks
e. Different percent of HPLC peak areas
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The correct answer is: Different retention times of HPLC peaks

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The “dead time (t0)” is retention time of a sample component that

Select one:
a. Moves slowest of all sample components
b. Has no affinity for the stationary phase
c. Has strong interaction with stationary phase functional groups
d. Has no interaction with mobile phase
e. Moves fastest of all sample components
Feedback

The correct answer is: Has no affinity for the stationary phase

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Number of theoretical plates can be practically determined by

Select one:
a. Passing ultrasonic waves through the column
b. Passing electromagnetic radiations through the column
c. Having microscopic view of column
d. Mathematical calculations of chromatogram
e. Microscopic analysis of stationary phase particles
Feedback

The correct answer is: Mathematical calculations of chromatogram

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The electrophoretic mobility of sample components depends upon many factors,

except
Select one:
a. Size and shape of molecule
b. Strength of electrical field
c. Properties of supporting medium
d. Concentration of molecule
e. Net charge on molecule
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The correct answer is: Concentration of molecule

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A low value of retention factor (k) indicates that sample component
Select one:
a. Poorly retained by stationary phase
b. Has low solubility in mobile phase
c. Highly retained by stationary phase
d. Moves very slowly along with mobile phase
e. Has high retention time
Feedback

The correct answer is: Poorly retained by stationary phase

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The straight line for term A in the van Deemter plot indicates that
Select one:
a. Peak broadening is unaffected by the mobile phase velocity
b. The mobile phase velocity does not influence the Eddy Diffusion
c. Multiple pathway diffusion does not influence the mobile phase velocity
d. Peak broadening is unaffected by Eddy Diffusion
e. Height of theoretical is unaffected by Eddy Diffusion
Feedback

The correct answer is: The mobile phase velocity does not influence the Eddy
Diffusion

Question 21
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During injection of sample solution into HPLC instrument, when knob of injection port
is at “loading position” then sample solution is
Select one:
a. Being carried by mobile phase to the column
b. Moving toward guard column
c. Moving towards solvent reservoir
d. Being filled in the sample loop
e. Going to wastage
Feedback

The correct answer is: Being filled in the sample loop

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In protein analysis by capillary electrophoresis, adsorption of protein to the wall of

capillary leads to smearing of protein that results in
Select one:
a. Higher retention time
b. Sharp peaks
c. Peak broadening
d. Widely separated peaks
e. Almost merged peaks
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The correct answer is: Peak broadening

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High speed of analysis of HPLC corresponds to

Select one:
a. Using macroporous stationary phase particles
b. Using microporous stationary phase particles
c. Using porous layer beads as stationary phase
d. Very fine particle size of stationary phase
e. Pressurized flow of mobile phase solvent
Feedback

The correct answer is: Pressurized flow of mobile phase solvent

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The most preferable separation method will be gas chromatography, when sample
is
Select one:
a. Having low vapor pressure
b. Heat sensitive
c. Thermally unstable
d. Non-Volatile
e. Volatile
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The correct answer is: Volatile

Question 25
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Only a few peaks in HPLC chromatogram represent

Select one:
a. Highly impure sample
b. High concentration of sample components
c. Pure sample
d. Sample is homogenous
e. Low concentration of sample components
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The correct answer is: Pure sample

Question 26
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If stationary phase particle size distribution is large, chromatographic peak

becomes broader due to high value of
Select one:
a. Longitudinal Diffusion
b. Mobile phase flow rate
c. Eddy Diffusion
d. Number of Theoretical plates
e. Mass transfer under non-equilibrium
Feedback

The correct answer is: Eddy Diffusion

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At each equilibrium stage, in the column, the component whose greater number of
molecules are in the mobile phase, will have
Select one:
a. Sharp Peak
b. High retention time
c. Low theoretical plate height
d. Low retention time
e. High number of theoretical plates
Feedback

The correct answer is: Low retention time

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One, which is not requirement of mobile phase of HPLC
Select one:
a. Free from dissolved gases
b. Free from any microbe
c. Must give strong detector signal
d. Free from any particulate matter
e. Non-reactive towards sample components
Feedback

The correct answer is: Must give strong detector signal

Question 29
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Plate theory describes the ______ effect of matrix components on sample analyte
during chromatographic elution.
Select one:
a. Either retarding or reinforcing effect
b. Retarding effect
c. Neither retarding nor reinforcing effect
d. Reinforcing effect
e. None of these
Feedback

The correct answer is: Neither retarding nor reinforcing effect

Question 30
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When target analyte is present in considerably high concentration, the preferable

sample injection method is
Select one:
a. Direct Vaporization Injection
b. Split injection
c. Splitless Injection
d. Cold on-column injection
e. Microliter pipette
Feedback

The correct answer is: Split injection

Question 31
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In capillary electrophoresis, negatively charged ions move towards cathode under

the effect of
Select one:
a. Electro osmotic flow
b. Repulsion by HO- ions
c. Concentration gradient
d. pH gradient
e. Electrophoretic mobility
Feedback
The correct answer is: Electro osmotic flow

Question 32
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Straight line for factor A in the van Deemter Plot indicates that HETP
Select one:
a. Decreases with increasing velocity of mobile phase
b. Have fix contribution by eddy diffusion which is independent of mobile phase
velocity
c. Remains unaffected by increasing velocity of mobile phase
d. Is not effected by eddy diffusion
e. Increases with increasing velocity of mobile phase
Feedback

The correct answer is: Have fix contribution by eddy diffusion which is independent
of mobile phase velocity

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The major function of guard column is

Select one:
a. To boost up the function of detector
b. helps to maintain a steady elution rate
c. To capture target analyte and separate it from rest of sample components
d. Increase lifetime of analytical column
e. Increase flow rate of mobile phase
Feedback

The correct answer is: Increase lifetime of analytical column

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The sample component that has only smaller height for its theoretical plates in a
given chromatographic column, will show
Select one:
a. Greater interaction to Mobile Phase
b. Greater interaction to Stationary Phase
c. Very small number of theoretical plates in the given column
d. Only a few binding functional groups on stationary phase
e. Very fast flow rate along with mobile phase
Feedback

The correct answer is: Greater interaction to Stationary Phase

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In GC, volume of carrier gas required for a particular analyte to completely elute
through the chromatographic system under set conditions, is known as
Select one:
a. Solute-gas ratio
b. Void volume
c. Analytical volume
d. Retention volume
e. Critical Volume
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The correct answer is: Retention volume

Question 36
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Which one of the followings, is not the basic requirement of support material used in
packed column in gas chromatography
Select one:
a. It should have large surface area
b. It should be chemically inert
c. It should be heat stable
d. It should be thermolabile
e. It should get completely wet with liquid stationary phase
Feedback

The correct answer is: It should be thermolabile

Question 37
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Question text

The separation of low molecular weight gases is achieved by gas solid

chromatography, in which the most preferable column is
Select one:
a. Glass column
b. Packed column
c. Capillary column
d. Open tubular column
e. Linear column
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The correct answer is: Packed column

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H2 is mostly, not the choice for mobile phase in GC because of its

Select one:
a. High Flow Rate
b. Low Molecular Wt.
c. Thermal conductivity
d. Low Density
e. Inflammable nature
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The correct answer is: Inflammable nature

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The process of electrophoretic separation begins with ________ as initiation step

Select one:
a. None
b. Sample application
c. Application of voltage
d. Generation of sample wells
e. Equilibration of electrophoretic medium
Feedback

The correct answer is: Equilibration of electrophoretic medium

Question 40
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The mobile phase in electrophoretic separations, is mostly a

Select one:
a. Molten salt
b. Organic solvent
c. Liquid of low polarity
d. Buffer of variable ionic strength
e. Gas
Feedback

The correct answer is: Buffer of variable ionic strength

 Started on Friday, 24 April 2020, 8:00 PM
State Finished
Completed on Friday, 24 April 2020, 8:10 PM
Time taken 9 mins 46 secs
Grade 7.00 out of 10.00 (70%)
Question 1
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The separation of sample components in a chromatographic column depends

upon

Select one:
a. Rf v alue
b. Variation in distribution co-efficient, K
c. Mobile Phase
d. None
e. Stationary Phase
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Your answ er is incorrect.

The correct answ er is: Variation in distribution co-efficient, K

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Paper chromatography is a type of

Select one:
a. Adsorption
b. Planar
c. I on Exchange
d. None
e. Column
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The correct answ er is: Planar

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greater number of HPLC peaks, represent higher ______ of the sample.

Select one:
a. number of impurities
b. Purity
c. high concentration of sample component
d. None
e. All of abov e options
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The correct answ er is: number of impurities

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Purging can be effectiv ely performed by bubbling ______ gas through mobile phase
liquid.

Select one:
a. N2
b. None
c. Ar
d. CH4
e. He
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The correct answ er is: He

Question 5
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Pressure of gas is used to generate constant, uniform flow rate in ________ pumps

Select one:
a. Syringe
b. Reciprocating
c. Pneumatic
d. All of these
e. None
Feedback

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The correct answ er is: Pneumatic

Question 6
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Testing capacity of per-packed HPLC column can be enhanced by

Select one:
a. decreasing mobile phase flow rate
b. using non-polar mobile phase
c. using gradient elution
d. using guard column
e. None
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The correct answ er is: using guard column

Question 7
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Percentage of a particular analyte in a giv en sample is monitored by ________of

HPLC peak.

Select one:
a. Peak Area
b. Retention Time
c. I ntensity
d. None
e. Retention Volume
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The correct answ er is: Peak Area

Question 8
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Porous layer beads are less satisfactorily applied under ______ pressure

Select one:
a. Medium
b. All of these
c. High
d. None
e. Low
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The correct answ er is: High

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Greater v alue of Rf means, _______ w ill be retention by stationary phase.

Select one:
a. Lesser
b. All of these
c. no relation
d. None
e. Higher
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The correct answ er is: Lesser

Question 10
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Most sensitiv e HPLC detector is

Select one:
a. None
b. Fluorescence Detector
c. UV/VI S
d. Differential Refractometric Detector
e. Thermal Conductiv ity Cell
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Your answ er is correct.
The correct answ er is: Fluorescence Detector

Started on Saturday, 9 May 2020, 8:47 PM

State Finished
Completed on Saturday, 9 May 2020, 8:59 PM
Time taken 12 mins 11 secs
Grade 5.00 out of 10.00 (50%)
Question 1
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GC w ill not be the most preferable method for separation of sample components,
w hen sample is

Select one:
a. Thermally unstable
b. Volatile
c. Heat Sensitiv e
d. Both (b) and (c)
e. All (a) , (b) and (c)
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The correct answ er is: All (a) , (b) and (c)

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The mobile phase in GC mov es the sample components along w ith it, by______

Select one:
a. Chemical reaction
b. Physical interaction
c. Dissolv ing sample components
d. Both (b) and (c)
e. All (a), (b) and (c)
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The correct answ er is: Both (b) and (c)

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Most commonly, GC is run in ______ mode

Select one:
a.
Rev erse
b.
Partition
c.
Adsorption
d. Forw ard
e. GSC
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The correct answ er is: Forw ard

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The statement “Mobile phase should giv e a sharp signal in the detector” is

Select one:
a. True
b. False
c. ___
d. ___
e. ___
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The correct answ er is: False

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H2 can be used as Mobile phase, mostly because of

Select one:
a. High Thermal conductiv ity
b. High Flow Rate
c. Low Molecular Wt.
d. Low Density
e. All of These
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The correct answ er is: All of These

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For thermally unstable compounds, the preferable sample introduction method is

Select one:
a. Cold on-column injection
b. Splitless I njection
c. Direct Vaporization I njection
d. Split injection
e. None
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The correct answ er is: Cold on-column injection

Question 7
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The most commonly used filling of WCOT-capillary columns is

Select one:
a. Kieselguhr
b. Porous polymers
c. None
d. Diatomaceous earth
e. Glass beads
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The correct answ er is: None

Question 8
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Operating temperature range for an ideal GC detector is

Select one:
a. 0-100 oC
b. 0-200 oC
c. 0-300 oC
d. 0-400oC
e. 0-500 oC
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The correct answ er is: 0-400oC

Question 9
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N2 can not be satisfactorily used as carrier gas w ith

Select one:
a. TC cell
b. FI D
c. ECD
d. Flame Photometric Detector
e. None
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The correct answ er is: ECD

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The coal based products can be best analyzed by

Select one:
a. TC cell
b. None
c. ECD
d. FI D
e. Flame Photometric Detector
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Your answ er is incorrect.

The correct answ er is: Flame Photometric Detector

Started on Saturday, 16 May 2020, 8:45 PM

State Finished
Completed on Saturday, 16 May 2020, 8:57 PM
Time taken 12 mins 22 secs
Grade 8 out of 10 (80%)
Question 1
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Question text

The principle of electrophoresis closely relates to

Select one:
a. Chromatography
b. Filtration
c. Coagulation
d. Sublimation
e. Solv ent Extraction
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The correct answ er is: Chromatography

Question 2
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Most commonly used v isualizing agent for DNA and proteins in Gel electrophoresis,
is

Select one:
a. Ethidium Bromide
b. Silv er Bromide
c. Barium Bromide
d. Aluminium Bromide
e. Vanidium Bromide
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Your answ er is correct.
The correct answ er is: Ethidium Bromide

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I n gel electrophoresis, gel serv es as

Select one:
a. Supporting medium
b. Molecular Siev e
c. Separation Medium
d. All of these
e. None
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The correct answ er is: All of these

Question 4
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The rate of migration of an ion in electrical field depends on

Select one:
a. Net charge of molecule
b. Size and shape of particle
c. Strength of electrical field
d. Properties of supporting medium
e. All of these
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The correct answ er is: All of these

Question 5
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The major function of buffer, in electrophoresis, is to

Select one:
a. Carry applied electric current
b. Control extent of solute ionization
c. Maintain pH of eletrophoretic experiment
d. Carry the electrophoretic mobility
e. All of these
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The correct answ er is: All of these
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The first step in electrophoretic operation is

Select one:
a. Sample application
b. Generation of sample w ells
c. Application of v oltage
d. None of These
e. Equilibration of electrophoretic medium
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The correct answ er is: Equilibration of electrophoretic medium

Question 7
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The ________ of gel is of prime importance, w hile selecting a gel for a giv en
electrophoretic separation

Select one:
a. Polarity
b. Pore size
c. Cross linking agent
d. Degree of polymerization
e. Manufacturer
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The correct answ er is: Pore size

Question 8
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The separation of sample components doesn’t depend upon

Select one:
a. Size of molecules
b. Strength of electrical field
c. Shape of molecules
d. Net charge of molecule
e. Optical Properties of molecules
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The correct answ er is: Optical Properties of molecules

Question 9
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The major necessary components of an electrophoretic equipment include:

Select one:
a. Pow er Pack
b. Electrophoresis Unit
c. Gel System
d.
Both (a) and (b)
e. Both (b) and (c)
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The correct answ er is:
Both (a) and (b)

Question 10
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I n a normal paper electrophoresis, the paper is

Select one:
a. Hanged in air
b. Sticked ov er aluminium sheet
c. Hanged in a tank
d. Placed betw een glass plates
e. Placed betw een plastic plates
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Your answ er is correct.

The correct answ er is: Placed betw een glass plates

Started on Saturday, 6 June 2020, 8:30 PM

State Finished

Completed on Saturday, 6 June 2020, 8:45 PM

Time taken 14 mins 45 secs

Grade 7 out of 10 (70%)

Question 1
Correct

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The rate of migration of an ion in electrical field does not depend on

Select one:

a. Optical activ ity of molecule

b. Size molecule

c. Shape of molecule

d. Strength of electrical field

e. Charge density of molecule

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The correct answ er is: Optical activ ity of molecule

Question 2
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The ampholyte compounds become strongly negativ e charged, w hen medium is

buffered at pH

Select one:

a. 11

b. 9

c. 7

d. 2

e. 5
Feedback

The correct answ er is: 11

Question 3
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The component of sample mixture, for w hich height of theoretical plate has
maximum v alue, w ill hav e

Select one:

a. Greater number of binding functional groups on stationary phase

b. Greater interaction to Stationary Phase

c. High Retention Time

d. Greater interaction to Mobile Phase

e. High Retention Volume

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The correct answ er is: Greater interaction to Mobile Phase

Question 4
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The separation among different sample species takes place according to their
charge to mass ratio in

Select one:

a. Chromatofocusing

b. Electrophoresis

c. Mass spectrometry

d. Both mass spectrometry and electrophoresis

e. All mass spectrometry, electrophoresis and chromatofocusing

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The correct answ er is: Electrophoresis

Question 5
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At each equilibrium stage, in the column, the component w hose greater number of
molecules are in the stationary phase, w ill hav e

Select one:
a. High theoretical plate height

b. Broad Peak

c. High retention time

d. Low theoretical plate v alue

e. Low retention time

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The correct answ er is: High retention time

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The major roles of buffer in electrophoretic separation include all except

Select one:

a. Controlling the physical state of supporting medium

b. Maintenance of electric current flow

c. Maintenance of electrophoretic mobility

d. Controlling the extent of ionization of sample molecules

e. Maintenance of experimental pH

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The correct answ er is: Controlling the physical state of supporting medium

Question 7
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The v alue of retention factor (k) w ill be high, if v alue of

Select one:

a. tR is small, w hile to is high

b. tR is small

c. to is high

d. Both tR and to are small

e. to is small, w hile tR is high

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The correct answ er is: to is small, w hile tR is high

Question 8
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The v alue of separation factor (α) greater than 1, but lesser than 1.2 corresponds to

Select one:

a. Possible separation

b. Difficult separation

c. No separation

d. Easy separation

e. Very difficult separation

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The correct answ er is: Very difficult separation

Question 9
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The time required by the mobile phase to pass through the column of stationary
phase is know n as

Select one:

a. Retention time of mobile phase

b. Hold up v olume

c. Dead time

d. Tm
e. tm

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The correct answ er is: Dead time

Question 10
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The mobile phase in electrophoretic separations, is mostly a

Select one:

a. Organic solv ent

b. Molten salt

c. Buffer of v ariable ionic strength

d. Gas

e. Liquid of low polarity

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The correct answ er is: Buffer of v ariable ionic strength