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Analytical Instrumentation Questions and

Answers – High Pressure Liquid
Chromatography
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This set of Analytical Instrumentation Multiple Choice Questions & Answers (MCQs) focuses on
“High Pressure Liquid Chromatography”.

1. Which of the following is not true about High pressure liquid chromatography (HPLC)?
a) It requires high pressure for the separation of the specious
b) There is no need to vaporise the samples
c) It is performed in columns
d) It has high sensitivity
View Answer

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2. High pressure liquid chromatography can be performed only in columns.

a) True
b) False
View Answer

3. Which of the following is not an advantage of Syringe type pumps used in High pressure liquid
chromatography?
a) Independent of viscosity
b) Pulse-less ow
c) High pressure capability
d) Unlimited solvent capacity
View Answer

4. Which of the following is not true about solvent programming which is done in high performance
liquid chromatography?
a) It provides unequal bandwidths
b) It provides fast overall separation
c) It provides maximum resolution
d) It provides maximum sensitivity
View Answer
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5. Which of the following pulse damper takesprotocols
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amount of the pulsation energy which is
SN Experiments
released to provide smooth pressure without pulsations?
a) Flexible bellows or compressible gas passed through tee columns LEARN MORE
b) Flexible inert diaphragm
c) Electronic pulse damper
d) Electrical pulse damper
View Answer

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6. Which of the following is not a characteristic of the syringe pump used in high pressure liquid
chromatography?
a) Pressure capability is high
b) Maintenance is frequent
c) Limited reservoir capability
d) Slight change of ow rate when extremely high pressure compresses the solvent
View Answer

7. Syringe pumps used in High pressure liquid chromatography are most suitable for which of the
following columns?
a) Capillary columns
b) Guard columns
c) Short-fast columns
d) Small bore columns
View Answer

8. Gravity feed method for solvent delivery is not used with narrow bore columns packed with ne
mesh particles.
a) True
b) False
View Answer

9. Which of the following cannot be done to reduce ripple in High pressure liquid chromatography?
a) Using bellows
b) Using restrictors
c) Using long nylon tube between pump and column
d) Avoiding the use of the solvent pump
View Answer

10. Which of the following is not true about Hydraulic capacitance ow control system used in
HPLC?
a) It can be used only for liquids with low viscosity
b) It is irrespective of solvent compressibility
c) It maintains a constant ow
d) It smoothens high pressure pump pulsations
View Answer

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Sanfoundry Global Education & Learning Series – Analytical Instrumentation.

To practice all areas of Analytical Instrumentation, here is complete set of 1000+ Multiple Choice
Questions and Answers.

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