Name: Bibek biswakarma Date: 21th march 2021

Student id: 19-2-02464 Course: BS BIO3-1

Enzymes

Answer on a separate sheet.

1. Indicate whether each of the following characteristics of an enzyme is true or false.

An enzymes
1._True ____ is a biological catalyst.
2._false____ functions at a low pH
3._True____ does not change the equilibrium position of a reaction.
4._True is obtained from the diet.
5._True greatly increases the rate of a cellular reaction.
6. True is needed for every reaction that takes place in the cell
7._false____ catalyzes at a faster rate at higher temperatures
8._True____ functions best at mild conditions of pH 7.4 and 37 oC
9. True lowers the activation energy of a biological reaction
10._false ___increases the rate of the forward reaction, but not the reverse.

2. Match the common name of each of the following enzymes with the description of the reaction.
a. dehydrogenase b. oxidase c. peptidase d. decarboxylase e. esterase f. Transaminase

1. Esterase hydrolyzes the ester bonds in triacylglycerols to yield fatty acids and glycerol.
2. Dehydrogenase removes hydrogen from a substrate
3. Decarboxylase removes CO2 from a substrate
4. Dehydrogenase _decomposes hydrogen peroxide to water and hydrogen
5. Peptidase _ hydrolyzes peptide bonds during digestion of proteins
6. Transaminase transfers an amino group from an amino acid to an alpha keto acid.

3. Match the IUPAC classification for enzymes with each of the following types of reactions.
a. oxidoreductase b. transferase c. hydrolase d. lyase e. isomerase f. ligase

1. Ligase combines small molecules using energy from ATP

2. Oxidoreductase hydrolyzes a disaccharide into two glucose units
3. Isomerase converts a substrate to an isomer of the substrate
4. Hydrolyse adds hydrogen to a substrate
5. Transfarase transfers phosphate groups
6. Oxidoreductase removes water from a substrate
7. Lyase adds oxygen to a substrate
8. Isomerase converts a cis structure to a trans structure
4. How is the rate of the urease-catalyzed reaction (optimum pH=5) affected by each of the following.
Urease catalyzes the hydrolysis of urea in the liver.
a. increases b. decreases c. not changed

1. Increase adding more urea when an excess of enzyme is present

2. Decrease running the reaction at pH 8
3. Not changed lowering the temperature to zero degree Celcius
4. Decreases running the reaction at 85 oC
5. Not changed increasing the concentration of urea for specific amount of urease.