Sapphire 350 Service Manual

Service Manual
WARNING .................................................................................................... 1
Installation........................................................................................................... 3
Moving the instrument ..................................................................................... 3
Unpacking .......................................................................................................... 4
Placing and preparing the instrument......................................................... 5

1 Technical specifications ......................................................................................... 7
General: ................................................................................................................ 7
Optical System:.................................................................................................... 7
Hydraulic System: ................................................................................................ 7
Reagent tray: ....................................................................................................... 7
Cuvette washer: .................................................................................................. 7
Software: ............................................................................................................... 8
Modes of analyses: ............................................................................................. 8
Electrical Requirements:..................................................................................... 8
Weight: .................................................................................................................. 8
2 Connections ............................................................................................................. 9
2.1 Connection to electrical supply: .................................................................. 9
2.2 Connection to a computer ........................................................................... 9
2.3 Fluid Connection ...........................................................................................10
3 Introduction ............................................................................................................11
3.1 Modules functional description ..................................................................11
3.1.1 Description of Sapphire autoanalyzer .................................................11
Sample and reagent tray ............................................................................11
Dispensing arm...............................................................................................12
Cuvettes tray or reaction tray.....................................................................12
Hydraulic system ............................................................................................12
Optical system................................................................................................13
3.1.2 Description of the working cycle ..........................................................14
Reaction preparation cycle........................................................................14
Cuvette washing cycle ................................................................................15
Reading cycle................................................................................................15
3.2 Operation from a terminal program..........................................................15
3.3 Directions for Sapphire’s mounting and dismounting ............................18
Module mounting and dismounting..............................................................18
Dismounting the cabinet .................................................................................18
ROBOT/HEAD Module.......................................................................................18
SAMPLE / REAGENT MODULE...........................................................................19
CUVETTE MODULE ..............................................................................................20
Washer Module..................................................................................................20
Sapphire 350 Service Manual - SSM0405 I

Photometer Module..........................................................................................21
Dilutor Module....................................................................................................21
Electronic Module .............................................................................................22
Hydraulic Mode .................................................................................................23
Adjustment sequence for each movement of the modules....................24
1. Calibrating reaction tray dispensing offset ..........................................25
2. Calibrating the cuvette base of washer...............................................25
3. Calibrating reaction tray photometer offset .......................................26
4. Adjusting robot arm’s reference position .............................................26
5. Calibrating vertical movement of the funnel base ............................27
6. Calibrating robot head’s position over dispensed .............................27
7. Calibrating vertical movement cuvette’s base ..................................28
8. Calibrating robot head’s position over reagent A .............................28
9. Calibrating reagent A container base of vertical movement .........29
10. Calibrating robot head position over reagent B...............................30
11. Calibrating reagent B container base of vertical movement........31
12. Calibrating robot head position over sample tube..........................32
13. Calibrating sample tubes vertical movement base.........................33
14. Calibrating volume meter of the washing container.......................33
15. Calibrating volume meter in the waste container ...........................34
16. Calibrating volume meter in the concentrated waste container.34
3.4 Changing the lamp ......................................................................................35
3.5 Changing interferential filters ......................................................................36
3.6 Changing the Sample / Reagent probe ..................................................37
3.7 Changing the TECAN syringe...................................................................39
3.8 Changing tubings..........................................................................................40
Changing the hydraulic circuit tubings. .......................................................40
Changing Peristaltic Pump tubing .................................................................41
3.9 Changing the cuvettes ................................................................................42
3.10 Obtaining parameters from firmware values...........................................43
4 Maintenance program.........................................................................................43
4.1 Daily Maintenance..............................................................................44
4.2 Weekly maintenance .........................................................................44
4.3 Six-monthly maintenance ..................................................................44

5 Troubleshootings. ...................................................................................................45
5.1 Malfunction problems displayed on the screen......................................45
5.2 Log.html...........................................................................................................46
5.3 Fast Test ...........................................................................................................50
5.4 Visible Flaws ....................................................................................................51
5.5 Inconsistent Measurements .........................................................................52
5.6 Inaccurate results in all analytes ................................................................52
Sapphire 350 Service Manual - SSM0405 II

a) Originated in the dilutor’s hydraulic system ........................................52
b) Originated in the cuvette washer .........................................................52
c) Originated by photometer and cuvettes ............................................52
5.7 Inaccurate results in some of the analytes ...........................................53
Kinetic and fixed-point kinetic methods ...................................................53
Final point methods.......................................................................................53
5.8 Inaccurate results due to faulty reagents, controls or samples ........53
5.9 Comunication Protocol for Technical Service use..................................54
Introduction ........................................................................................................54
Message Structure.............................................................................................54
Command Description.....................................................................................54
5.10 EEPROM version 1.02 directions ..................................................................61
6 Validation Program .......................................................................................65
6.1 Photometrical Parameters to Control........................................................65
6.2 Spurious light ...................................................................................................65
6.3 Photometrical Stability..................................................................................67
6.4 Photometrical Accuracy..............................................................................68
6.5 Photometrical Precision................................................................................69
6.6 Photometrical Linearity.................................................................................70
7 Diagrams .........................................................................................................72
7.1 Identifying printed circuits............................................................................72
7.2 Electrical and electronic scheme ..............................................................74
7.3 Mechanical scheme...................................................................................110
7.4 Hydraulic Scheme .......................................................................................117
APÉNDIX A: Spare parts list ....................................................................................120
Sapphire 350 Service Manual - SSM0405 III

WARNING
1) The instrument must be installed by specialized personnel only.
2) This instrument is Class 1, Type b, IPX1.
3) Environmental requirements for operation: temperature: 15 – 30º C;
humidity: 40 – 85% (no condensation).
4) The instrument must be connected to a supply line according to
standing regulations.
5) The instrument must not be used for a purpose other than that it was
designed for.
6) Wait for 10 minutes after switching on the instrument to start the first
determinations.
7) Do not switch the instrument on and off repeatedly. Wait for at least
20 minutes before restarting.
8) If the line voltage is lower than 200 Volts within the scope 200 ~ 240
VCA or lower than 100 volts within the scope 100 ~ 140 VCA, use a
resonant voltage stabilizer or a 1500 Watt sinusoidal output
uninterruptible power supply (UPS).
9) Read the User’s Manual before starting using the instrument.
Sapphire 350 Service Manual - SSM0405 1

Symbols used in the manual and instrument
Attention
High voltage
Ground Connection
Warning about the instrument and laboratory practice
1) The use of calibrators is recommended, no matter which method is

used, including those methods where the reagent’s manufacturer
uses a factor.
2) The use of controls is recommended, as it is indicated by good
laboratory practice.
3) Read every message at the end of each run. The results may or may
not be accepted by the user, depending on the reagent
deterioration index, on its linearity in kinetic methods, etc.
4) Perform the maintenance plan as indicated in this manual.

Installation
Instrument installation must be performed by especially trained personnel.
Electrical requirements: a standard 220 Volts 50 Hz or 110 Volts 60 Hz socket

for 350 VA consumption. The supply line has a third terminal to ensure
proper ground connection. If line variations are higher than 10%, a stabilizer
of the kind ferro-resonant is recommended, or else a 500 VA
uninterrumpible power supply (UPS).
Kind of water: bidistilled, approximate consumption 1.6 ml/det.
Room temperature: 18 – 25 °C
Relative humidity: 40 – 85% (no condensation)
Moving the instrument
Avoid knocking and/or vibration of any kind while moving.

Unpacking
Perform the unpacking procedure with extreme care. Once this procedure
is finished, place the equipment on the table. The equipment is packed
inside a wodden box and it is held by bands. Proceed according to the
following procedure:
Band
Screwed
bottom
1) Cut the bands and remove the screws on the box’s bottom.
2) Remove box’s lid by carefully lifting it.
Band

3) Cut the band fixing the instrument to the bottom.
4) Remove plastic film protecting the instrument.
Hoses inside
5) Remove cardboard protecting the hoses.
Placing and preparing the instrument
The instrument must be placed onto a table in a clean environment, free

from dust and corrosive vapours to ensure its proper operation for a long
time. Leave a 20- centimeter separation between the equipment and the
wall in order to ensure good ventilation. Liquid containers must be under
the table. Avoid collapsing of the water entrance and waste exit tubings.
The instrument must not undergo any kind of vibration or sudden
temperature variations.
Avoid any centrifuge, lifts or x-ray supply lines or any other kind of noise-
generating apparatuses in the supply line.
Avoid direct sun light or illumination on the instrument.
The supply line has a third ground conductor for protection purposes.

WARNING: Make sure that ground connection in the line fulfills the
standard requisites for its operating power. Not performing ground
connection involves life risk for the operator and may damage one or
several parts of the equipment or the computer.
Interconnect the instrument to the computer and its peripherals before

connecting to the supply line.
Good working order as well as the quality of results may be affected if the
previous requirements are not fulfilled.

1 Technical specifications
General:
• Maximum working speed: 360 det/ hour.
• Average speed: 300 det/hour.
• Automatic, random access and discrete.
Optical System:
• Double beam.
• Interferential Filters: 340,380,405,450,505,546,578,600,650,700.
• Quartz – hallogen lamp. 12 volts 20 watts.
• Optical path: 6 mm.
• Photometrical scope: - 0.100 a 3.600 A.
• Type of measurement: Monochromatic and bichromatic.
Hydraulic System:
• High resolution dilutor.
• Sample volume: 2 to 200 µl
• Average reagent consumption: 250 µl/det.
• Minimum consumption: 200 µl/det.
• Inside and outside probe washing.
Sample and reagent tray:

• Reagent positions: 60.
• Double- mouth reagent flasks for two-reagent methods.
• Continuous sample loading ( 60-tube physical capacity).
• Admits primary tubes and pediatric sample tubes.
• Reagent cooling 15°C below room temperature. Independent on/off
switch.
Reagent tray:
• PMMA reaction cuvettes.
• Number of cuvettes: 100
• Thermal incubation by air bath.
• Working temperature: 37 ºC
Cuvette washer:
• Water consumption: 1.6 ml/det. aprox.

Software:
• Loading of patients, calibrators and controls during run.
• Handling of interferences.
• Urgencies.
• Easy method adaptation. Similar to hand work.
• Unlimited method number in memory.
• Calibration curves with unlimited number of calibrators.
• Automatic sample redilution.
• Statistic calculation. Levy – Jennings plots.
• Data importing and exporting with administration programs.
• Data Records.
Modes of analyses:
• Endpoint with white of reagent.
• Endpoint with white of sample.
• Kinetics.
• Fixed times kinetics.
• Turbidity measurement/turbidimetry.
• Homogeneous immunoanaIysis.
Electrical Requirements:
• 110/220 Volts 50/60 Hz.
Measurements:
• Width: 80 cm. Height: 45 cm. Depth. 58 cm.
Weight:
• 45 Kg.

2 Connections
Interlock
2.1 Connection to electrical supply:

Connect the interlock shown to 110-220 V supply tension and 50-60 Hz
frequency.; connector RS232C to a PC having the following characteristics:
Microprocessor Pentium IV - 30GB - 128 MB RAM, independent video plate,
port series RS232, monitor SVGA, keyboard and mouse. Windows XP. The PC
must fulfill standard rules for electrical safety IEC 60950 and have a certified
source.
2.2 Connection to a computer

Connector RS232C to a PC having the following characteristics:
Microprocessor Pentium IV - 30GB - 128 MB RAM, independent video plate,
port series RS232, monitor SVGA, keyboard and mouse. Windows XP. The PC
must fulfill standard rules for electrical safety IEC 60950 and have a certified
source.

2.3 Fluid Connection
The tubings indicating “to waste” and “to washing solution” are connected
to the corresponding containers. See the container interconnection graph
in the hydraulic scheme(“Diagrams, section 6 of the Technical Service
Manual).

3 Introduction
3.1 Modules functional description
3.1.1 Description of Sapphire autoanalyzer
Sapphire autoanalyzer has been conceived with the latest technology in

communications, electronics and robotics. It is wholly controlled by a
microprocessor. All mechanical functions are performed and controlled by
depending processors, which allows permanent observation of the
hardware’s state, as well as analyses development on the user’s screen.
Sapphire autoanalyzer is divided in the following modules:

• Sample and reagent tray
• Dispensing arm
• Cuvette tray or reaction tray
• Cuvette washer
• Hydraulic system
• Optical system
Sample and reagent tray
This tray allows 30 reagents, all of them cooled. The minimum

reachable reagent temperature will be 4° C, and cooling will be
interrupted below that limit. Normal operation temperature will be
around 15 °C below room temperature. This means that, if room
temperature is 20 °C, reagent temperature will be 5 °C.
Every reagent flask can have a simple or a double mouth, thus
allowing the use of only one position in those methods having two
reagents.
The sample and reagent tray can include up tp 60 samples (physical
capacity), in Kahn tubes with a mouth diameter of 13 mm. The
software will allow permanent sample loading once the requested
determinations have been concluded.
The capillary for taking samples feels liquid level, stopping its way 1
mm. below the surface of the liquids. This will allow the use of primary
tubes -that is, tubes with the cellular package (clot) in its lower part
and the sample or plasm in the upper part- are allowed. This
increases biosafety in the laboratory.

Dispensing arm
The dispensing arm is the one in charge of transporting the reagent(s) and
the aspirated sample volume of the selected sample to a cuvette on the
reaction tray.
Sample and reagent are prethermostatized at 37ºC by the preheater
included in the dispensing arm, before being dispensed onto the tray.
The dispensing arm detects the reagent and sample’s level by conductivity.
The tip of the probe is protected by an impact detector. When this detector
is activated by an abnormal descent of the dispensing arm, it automatically
stops its movement, so as not to damage the tip of the probe by the
impact.
Cuvettes tray or reaction tray
This is the place where reactions will be performed and read. It has 100
individual cuvettes, grouped in strips of 10 cuvettes each.
Cuvettes are made of PMMA, a kind of material that allows optimal
transmittance in the UV. Every cuvette will be individually measured with
water during optical system calibration. This way, a white of cuvette will be
obtained.
This tray is heated by air bath at 37 ° C, the temperature all reactions will be
performed at.
Each cuvette will have a minimum measuring volume of 200 µl, an a
maximum volume of 400 µl.
It is advisable to change the cuvettes every 3.000 determinations.
Hydraulic system
• Dilutor
TECAN dilutor is in charge of aspirating the required volumes of
reagent and sample and dispenses them into the corresponding
cuvette. Allowed volumes of reagent to be aspirated range from 200
to 400 µl and those for sample, from 2 to 200 µl.
TECAN dilutor has a 3-way valve and a 500 µl syringe, made of
boron silicate glass. The piston is made of stainless steel and has a
Teflon seal.
• Dispensing unit
Its main components are the capillary (Sample/Reagent probe) and
the preheater on the Robot arm. There is a peristaltic pump which is
Interconnected through the TECAN dilutor. This pump aspirates the
washing solution from the container to wash the capillary’s inside and
outside in the funnel.

• Washer
It has to wash the cuvettes once reactions have taken place and
measured. The washer is divided into::
• Reaction/dispensing arm: It is made up of two stainless steel
capillaries located on the right extreme of the washing comb. One
of the capillaries aspirates the reaction and sends it to the
concentrated waste container- if there is one- or, if this container
does not exist, to the waste container. The other capillary fills the
cuvette with washing solution.
• Dispensing/Aspiration: These are 6 central capillaries on the
washing comb, which are mounted together: 3 capillaries
dispense washing solution; the other 3 capillaries aspirate what has
been dispensed.
• Drying Tubing: These are 2 capillaries located on the left extreme
of the washing comb. They will dry the cuvette that has already
been washed by means of a vacuum pump.
• Diaphragm pumps: They are used by the cuvette washing system.
They are in charge of aspirating the reaction and send it to the
concentration container, as well as of the dilution and washing
solution. The other set of diagram pumps is also used by the
cuvette washing system to send the washing solution to the
cuvettes.
Each cuvette is in time washed by about 1600 µl of washing solution.
Optical system
This system is made up of:
• Light source: A 12 volts 20 Watts halogen lamp is used, with a high

emission in the UV (320 nm – 380 nm)
• Collimating lenses: It uses a plane convex lense to collimate the
light beam emitted by the halogen lamp. The other lenses are
used for the Sample channel and for the Reference channel.
• Interferential filters: It has 11 interferential filters, which are mounted
on the filter wheel. Filter positioning and selection is made by
means of a step engine. The filter wheel is equipped with the
following wavelengths: 340 / 380 / 405 / 450 / 505 / 546 / 578 / 600 /
650 and 750 nm. One position stays free to be added at the user’s
choice.
• Light beam splitter : the collimated light beam goes through the
chosen interferential filter, which strikes on the Light beam splitter

so as to split the beam into two beams. One of these beams
continues its way to the reference channel (with a smaller light
percentage) and the other goes to the sample channel (with a
higher light percentage).
• Sample channel: The beam coming from the beam splitter which
has the higher light percentage is refocused by the lens to the
reaction cuvette. When it goes through it, it directly strikes on the
reference detector, which turns it into an electrical signal. This
signal becomes a digital signal, by means of the converter. The
signal obtained is proportional to the transmittance of the sample
reaction on the cuvette.
• Reference channel: the beam from the beam splitter with a
smaller light percentage is refocused by the lens on the reference
detector . The beam so focused is converted into an electrical
signal by the reference detector. This signal is converted into an
electrical signal by the reference detector. It indicates the lamp’s
energy over each wavelength selected at the moment of
performing measurements.
3.1.2 Description of the working cycle
The operating cycle can be divided in three parts:

• Reaction peparation cycle
• Reading cycle
• Cuvette washing and reaction dispensing cycle
Reaction preparation cycle
This cycle stages are:

1) The dispensing arm moves toward the sample and reagent tray and
stands over the reagent to be aspirated. Then the capillary of the
dispensing arm descends until it finds liquid level and it aspirates the
necessary reagent volume by means of the dilutor.
2) The dispensing arm moves toward the funnel, carrying out the
external washing of the capillary.
3) The dispensing arm moves toward the sample and reagent tray,
positioning itself on the sample to be aspirated. The capillary on the
dispensing arm then descends until it finds liquid level and aspirates
the necessary sample volume by means of the diluter.
4) The dispensing arm moves toward the cuvettes tray, standing in
position to dispense reagent + sample on the cuvette that has been
preselected by the Sapphire user’s program. While this reagent +
sample on the cuvette is going on, the mixture is produced, which
starts incubation time and subsequent reaction.

5) The dispensing arm moves toward the funnel, where internal washing
of the capillary will take place by means of the peristaltic pump, thus
becoming ready for the next cycle.
Cuvette washing cycle
While the cuvette tray is still, waiting for dispensing to finish, the
cuvette washer operates on the cuvettes that are positioned on its
operating area. These are cuvettes where reactions have already
been measured.
Reading cycle
The preparation cycle is simultaneous to the reading cycle which

consists of performing the photometrical reading of those reactions
requiring it, in their suitable wavelength, as required by Sapphire
user’s program and according to the work routine.
The time period when the sample is dispensed and the cuvettes are
washed is the only time reading can not be done.
3.2 Operation from a terminal program

The autoanalyzer can be operated from a terminal program. This will allow
us to perform Firmware calibrations as well as verify Hardware adjustments.
Firmware calibrations keep all the variables on each module. These

variables can be directly read from the Sapphire user’s program. To do so,
we should go to the user’s screen, select Maintenance, go to
Configuration, Memory and obtain Parameters for the Equipment.
Note: The variables kept in the memory are the values obtained by the
manufacturer. Do not modify. It can only be modified when the Module
needs to be replaced or when advised by the manufacturer.
Hardware adjustment consists in adjusting the Reference and Sample

preamplifiers of the Photometer Module Offset.
Note: This adjustment is only indicated when changing the preamplifiers.
We can also verify module operation, the sate of detectors according to

their position, the state of the Sample/Reagent Probe level sensor and the
abnormal descent of the Sample/Reagent probe from this program, as well
as obtain the Preheater and Heater reading temperature, controlling the

water dispensing volume in the cuvette washing station, check the
peristaltic pump and Dilutor’s operation.
Note: Once the Hyperterminal has been activated, we advise analyzing

response messages directly from the log.html file. Do not use the
Hyperterminal screen so as to avoid confusion or misinterpretation of the
messages that have been sent or received.
Important: make sure you have closed the Hyperterminal program before
opening the Sapphire autoanalyzer’s program.
In this chapter It will be explained how to configurate the Hyperterminal

program included in Windows.
From the program Windows click on:

Start / programs / accessories / communications / hyperterminal
Type Sapphire in the Name box and accept.
Select COM1 and accept.
Select 115200 bits per second, 8 data bits, No Parity, 1 Stop bit, and
Hardware Flow Control
After that, click on: File / Properties
Select the Settings tag.
Click on ASCII Setup… and check the following boxes:
ASCII Sending:
Send line end with line feeds
Echo typed characters locally
ASCII Receiving:
Append line feeds to incoming line ends
The software is already configured to command the autoanalyzer. On

future occasions, when you want to use this software, you will only need to
click on the Sapphire.ht icon that was generated in the previous
configuration and that is kept in

Start / programs / accessories / communications / hyperterminal /
Sapphire.ht

3.3 Directions for Sapphire’s mounting and dismounting
Module mounting and dismounting
Sapphire autoanalyzer has several modules, each of which can be easily

dismounted to perform total or partial change.
Important: before dismounting, turn the equipment off and disconnect the
interlock from the supply line. Remove reagents and put them away into the
freezer. Remove samples and proceed to decontaminate the equipment
and the waste according to biosafety rules set up by the laboratory.
Dismounting the cabinet
In order to access the modules, the following covers must be removed:
a. Remove Cuvette Tray and Sample/Reagent Tray covers.

b. Then proceed to remove the upper left and right side covers
(remove Allen M3 screws from back).
c. Turn the Robot Arm completely to the left.
d. Remove the table (by removing the 4 Allen M3 screws. There are two
on each side)
e. Remove the front cover, slightly move the equipment forward so as to
access to the screws (3 Allen M4 screws) from below. Then remove
the screws (Allen M3) from the right and left sides.
f. Finally, remove the side lids, move the equipment so as to be able to
remove the screws and so access from below (2 Allen M4 screws on
each side). Then, from the bottom part, remove the screw (Allen M4,
one on each side).
Important: Manually position the head on the funnel before turning on the
equipment.
ROBOT/HEAD Module
a. Disconnect the air connector, the cable (going to the cabinet)

holder and the teflon tubing going to the dilutor.
b. Disconnect the J3 connector of the PCB PREHEATER. Do not
disconnect the vertical and horizontal movement detector
connectors.
c. Disconnect connectors J3, J4, J5 and J6 of the PCB Controller.

d. Dismount the Robot/Head module, remove the 4 Allen M5 screws.
Two of these screws are on the upper part mounted on the module
base separator and the other two on the lower part, at the same
level of the general base.
e. No adjustment is required to mount the module. Fix it to the bottom
base by means of the four screws, fix the fixing screws firmly. Continue
with the mounting.
f. Perform a precision test (section 9, Validation Program) in order to
verify correct mounting of the arm. Priming of the hydraulic should
previously be made.
Note: The head can be dismounted without totally removing the Robot /
Head module from the autoanalyzer’s base (in order to do so, it is only
required to disconnect the air connector, the flex holder and the teflon
tubing going to the dilutor, by loosening the Allen M3 and M4 screws from
the cylindrical and rectangular pieces joining the head to the axis
respectively). Once it has been mounted, it is important to verify the right
positioning of the Sample/ Reagent probe in the dispensing position.
SAMPLE / REAGENT MODULE
a. Remove the Sample / Reagent Tray (remove the Delrin lid, then the 3
Allen M6 screws). Disconnect the cable from the J1 connector in the
PCB Cooler under the tray.
b. Remove the seals holding the cable (do not cut with pliers) on the
Sample / Reagent module. One is placed on the right of the engine
in the module, another one is located in the central part of the base,
next to the diaphragm pump module, another one on the back,
between the Sample / Reagent module and the Robot module,
another one on the base behind the robot and the other behind the
robot, half-way up next to the dilutor.
c. Disconnect J1 and J2 from the PCB Controller and the PCB Brush.
d. Remove tubing (PVC 6/10) from the collecting tray of the condensing
circuit of the Peltier reagent cooling circuit.
e. Remove the 6 (M6) nuts fixing the Sample / Reagent module (it has a
flat safety washer)
f. Remove the module.
g. No adjustment is required to mount the Sample / Reagent module.
h. Continue mounting.
NOTE: In order to change the detector from the Home Position and change
the synchronic belt, it is necessary to remove the whole module. To change
the belt, the detector must be removed first.

CUVETTE MODULE
This module, for its part, has the Photometer and Cuvette Washer module.
a. To remove the whole Cuvette module, the seals holding the wires
must first be removed. (do not cut with pliers). There is one behind the
robot on the base, another behind the robot half way up, next to the
dilutor, another on the base between the robot module and the
Sample / Reagent module, another one on the center, next to the
diaphragm pump module. The latter holds the cables of the Cuvette
washing module.
b. Disconnect connectors J9, J10, J11, J12, J15 (Pos.1 and 2 set of brown
and white wires), J16, J17 from the PCB Controller and connectors J2
and J8 from the PCB Regulator, corresponding to the set of wires on
the photometer’s side. Disconnect connectors J7 and J8 from the
PCB Controller, corresponding to the set of wires on the cuvette
washer’s side.
c. Disconnect the filling and aspirating tubings from the Washer.
d. Remove the 4 M6 fixing nuts on the Cuvette module (they have flat
safety washers)
e. Remove the module.
f. No adjustment is needed to perform complete mounting of the
Cuvette module.
g. Proceed to mount.
NOTE: To change the sensor from the Home position, it is necessary to

remove the cuvette supporting disk. This disk has only one position, which
makes its mounting easier. Dismount the resistor heater (the one in the
middle). Remove fixing screws of the Home sensor from the bottom side. To
remove the belt, it is also important to dismount the Home sensor.
To remove the Washer and Photometer modules, it is not necessary to
completely remove the Cuvette module.
Washer Module
a. Remove the seals holding the cables in the following locations: one is
between the Sample / Reagent module and the diaphragm pump
module, another one is on the base next to the robot, another one is
behind the robot and the other one is half way up, next to the dilutor.
b. Disconnect connectors J7 and J8 on the PCB Controller,
corresponding to the set of wires next to the cuvette washer.
c. Disconnect the filling and aspirating tubings on / of the Washer.

d. Remove the 2 Allen M6 screws mounted on the module base.
Remove the washer.
e. No adjustment is required for mounting the Washer module.
f. Once the Washer module has been mounted, perform several
washing cycles.
Photometer Module
a. Remove the seals holding the cables located: one next to the step
motor in the Cuvette module, another on the base behind the robot
and the other one half way up, next to the dilutor.
b. Disconnect connectors J9, J10, J16, J17 of the PCB Controller and
connector J8 of the PCB Regulator.
c. Remove the 2 Allen M5 screws mounted on the bottom at the level of
the base of the Cuvette module. Remove the Photometer module.
d. In order to mount the Photometer module, alignment of the optical
path in relation with reaction cuvette should be performed.
e. Once mounting and alignment of the optical path has finished, run
the Validation Program (see section 9)
f. Perform Photometer Calibration (for all cuvettes) from the user’s
program. Go to Maintenance, Equipment and select Photometer
Calibration.
Diluter Module
In order to remove the Diluter module it is necessary to dismount the

Cuvette Module or the Photometer Module first.
a. Remove the seals holding the cables (do not cut with pliers), placed
at the front of the PCB Controller’s holder.
b. Disconnect connector J23 of the PCB Controller and connector J5
(terminal 1 and 3 thin brown and black wires) of the PCB Regulator.
c. Dismount TECAN syringe and 3 –way valve.
d. Remove the 2 Allen (M3) screws with star washer and nut , located on
the bottom and behind the Robot module. Remove the two Phillips
screws with star washer and nut located on the upper front of the
autoanalyzer.
e. No adjustment is required for mounting this module.
f. Perform several priming cycles.

Electronic Module
It is located on the left back part of the autoanalyzer. The printed circuit
plates are mounted on a metal support. Interconnections between PCB
and the electrical supply are made through connectors.
The plates forming the electronic module are: PCB CONTROLLER and PCB
REGULATOR. There are also 24 Volts, 15 Volts and +/-15 Volts commutated
electrical supplies.
PCB
CONTROLLER
PCB
REGULATOR
PCB
PREHEATER
SW 15 VDC
PELTIER source
LINE FILTER
SW 24 VDC
MOTOR/DIGITAL
SW +/-15VDC source
Note: The Controller plate is mounted on the metal support by 5 Allen

screws. The Regulator plate is mounted by 4 Allen screws, two on each side.

Hydraulic Mode
Hydraulic circuit is divided in several modules; one of them is the Diaphragm

Pump module (Pure Emptying, Diluted emptying, Filling, Drying). Diaphragm
pumps are controlled by the PCB Controller. In order to disconnect them,
connector J27 on the plate must be disconnected.
The Flow Regulator is in this module, too. It is mounted at the exit of the
Filling Pump, together with the Check valves mounted on the Manifold.
The other module is the Dilutor, interconnected to the Peristaltic Pump on

one way and to the Preheater on the other way, by means of a 3- way
valve of the TECAN dilutor module. From the Preheater it is connected to
the Sample / Reagent probe by means of the teflon tubing to a hydraulic
connector. The Peristaltic pump is controlled by the PCB Controller. To
disconnect it, you should disconnect connector J13.
Finally, there is the Cuvette Washing Station module, which is mounted on

the Cuvette module.
Manifold
Check
valve
Membrane
Pump
Cuvette washing station
Flow regulator

Diaphragm pumps are located below the Sample / Reagent tray. Access is
achieved by the front of the Sapphire autoanalyzer. They are attached, at
the level of the general base, by two Allen M4 screws.
3 – way Valve
Dilutor
Peristaltic pump with

tubing
CAVRO/DILUTOR
syringe
Preheater
TECAN dilutor module is mounted on the front of Sapphire autoanalyzer.
Adjustment sequence for each movement of the modules
The calibration sequence of firmware parameters corresponding to the

different modules that make up the equipment is described in this section. It
is a referential sequence which can only be performed on the necessary
points.
In order to perform the adjustments it is important to consider the values
obtained from Firmware (see section 3.10 of the Technical Service Manual,
“Obtaining Equipment Parameters”). These values correspond to the
adjustments made in the factory.
Adjustment values for each module can also be consulted from
HyperTerminal, by executing the command eepromRead. We can enter
new adjustment values by means of the command eepromWrite, (see
section 5.10 of the Technical Service Manual to find the corresponding
EEPROM address for each variable.
Note: Activate the HyperTerminal program by exiting the User’s program.

Initialize all modules before checking or adjusting each module.

1. Calibrating reaction tray dispensing offset
This calibration adjusts the Reaction Tray’s position at the moment of

Dispensing by the Robot Arm into the reaction cuvette. The reference point
for this adjustment is the washer and this adjustment is independent from
the photometer’s calibration (Reaction tray photometer offset).
To verify adjustment of the Reaction Tray Dispensing we must take into

account that the drying tip of the washing station is positioned over
cuvette Nº9. Therefore, the other drying tip is placed over cuvette Nº10. This
gives us the reference position for the Reaction Tray.
Initialize Reaction Tray and Washer. Verify that the last drying tip of the
washing station is perfectly aligned with cuvette Nº9. Otherwise, correct the
position by executing the command reactionMoveForward or
reactionMoveBackward and enter the number of steps you want to move it
in a relative way, clockwise and anticlockwise respectively. Once the
wanted position is obtained , execute the command eepromWrite A1 82
XXX where XXX is the new position obtained. Restart the reaction tray and
repeat the operation if necessary.
Then execute the command washerIn, verify that the tips in the washing
station are introduced into the cuvette strip and are perfectly aligned in
relation to the cuvettes. By means of the command washerOut, the tips are
removed from the cuvette washer.
Important: It is advisable to check the Reaction Tray’s position in relation to

the Washer twice or three times. This will allow us to check the correct
position of the adjustment performed, as well as the stability of the Reaction
Tray’s movement.
Note: The polarizing pin for mounting the Reaction Tray is in line with the
center of the first cuvette strip. A complete turn of the Reaction Tray is
equal to 2400 steps. As the Reaction tray has a capacity for 10 strips, then
the number of steps per cuvette is 24.
2. Calibrating the cuvette base of washer
Initialize Reaction Tray and Cuvette Washer. Execute the command

reactionGoDispense A1 96. Remove the second cuvette strip (cuvette Nº11
and Nº21). Then execute the command washerIn to lower the Washer over
the reaction cuvettes. Verify the existence of a space neither bigger than
1mm nor smaller than 0.5mm between the cuvettes bottom and the

washer’s tips. Adjust this height by executing the command eepromWrite A1
315 XXX, where XXX is the distance, in steps, that the washer will move.
To put up the washer, execute the command washerOut. Check positions

once or twice. This will allow us to check the stability of the mechanical
movement as well as the correct Washer position in relation to the Reaction
Tray.
3. Calibrating reaction tray photometer offset
This calibration is important, since it acts on the adjustment of the Reaction

Tray in relation to the light beam on the Photometer ‘s Sample Channel. This
adjustment is independent from Dispensing adjustment (Dispensing offset).
Initialize the Reaction Tray. Before performing this adjustment, check the
position of the Reaction Tray against the Washer (reference position).
Execute the command reactionGoPhoto A1 1 to position cuvette Nº1

above the photometer. Check that the light beam is centered on the
cuvette.
To correct the position, execute the commands reactionMoveForward or

reactionMoveBackward and enter the steps you want to move. Once the
wanted position is obtained, execute the command eepromWrite A1 84
XXX, where XXX is the newly obtained position. Restart the reaction tray
and repeat the operation if necessary. Check that asynchronic response
referred to dispensing position is 0 (reactionCurDispense A1 PC 0).
Important: once the adjustment has been performed, it is advisable to

check positions twice or three times. This will allow us to check the correct
position of the adjustment performed as well as the stability of the Reaction
Tray’s movement.
4. Adjusting robot arm’s reference position
The reference position of the Robot Arm’s horizontal movement is that of

Funnel. To perform horizontal adjustment we should loosen the Allen M4
screw located on the square block on the Head. For a better visualization,
execute the command probeMoveIn by entering 200 steps as an
argument. Slightly turn the head to the right or the left until the probe is
positioned above the funnel center. Firmly adjust Allen screw.

Verify that EEPROM 50 position be equal to zero. Execute the commands
probeInit and probeArmInit to initialize the vertical and horizontal
movements of the Robot Arm.
Important: the Allen M3 screw mounted on the cylindrical piece should

adjust at the level of the head (Sample / Reagent probe’s support). If it is
modified, it should be checked that the probe’s height in relation to the
channel (area of probe displacement) on the general lid or table be 5 mm.
5. Calibrating vertical movement of the funnel base
To correct this level’s height, the number of steps needed to adjust the
Robot Arm in relation to the funnel should previously be established. This
value can be accurately established by means of the commands
probeMoveInWithoutLevel and probeMoveOut. Once the desired position
has been obtained, execute the command eepromWrite A1 18 XXX where
XXX is the newly obtained position.
Important: Every 10 steps, an advancement of 1 mm is obtained.
Note: once adjustment has been made, it is advisable to check positions

twice or three times. This will allow us to check the correct position of the
adjustment performed as well as the stability of the Robot Arm’s movement.
The purpose of this calibration is to adjust the robot Arm’s positioning over
the funnel.
6. Calibrating robot head’s position over dispensed
Execute the command to initialize the cuvette Tray and the commands to
initialize vertical and horizontal movement of the Robot Arm.
Then execute the command probeGoDispense; the Robot arm will directly
move towards the Cuvette Tray, positioning itself over the mouth on cuvette
Nº1.The probe must be perfectly aligned over the mouth. Otherwise,
positioning must be corrected on the horizontal movement of the robot
arm.
To adjust the horizontal position of the Robot arm, the commands

probeArmMoveClock and probeArmMoveCClock must be executed,
moving the necessary steps. Once the desired position has been obtained,
execute the command eepromWrite A1 48 XXX where XXX is the newly
obtained position.

To visualize better, execute the command probeMoveIn and enter 800
steps as argument. The probe must be centered in relation to the cuvette’s
mouth.
Note: Once the adjustment has been performed, it is advisable to check

positions twice or three times. This will allow us to check the correct position
of the adjustment performed as well as the stability of the Robot Arm and
the Cuvette Tray’s movement.
7. Calibrating vertical movement cuvette’s base
This calibration is meant to adjust vertical movement of the Robot Arm on

the reaction cuvette’s position at Dispensing.
Initialize Reaction Tray and Robot Arm, both vertical and horizontal and
execute the command probeGoDispense.
Execute the command probeMoveIn. The Sample / Reagent probe must

become positioned about 1 mm. over the reaction cuvette’s mouth.
To correct position, the number of steps needed to adjust the Robot Arm in
relation to the reaction cuvette’s mouth should previously be established.
This value can be accurately established by means of the commands
probeMoveIn and probeMoveOut. Once the desired position has been
obtained, execute the command eepromWrite A1 16 XXX, where XXX is
the newly obtained position.
Note: Once adjustment has been performed, it is advisable to check

of the adjustment performed as well as the stability of the Robot Arm’s
movement.
8. Calibrating robot head’s position over reagent A
The purpose of this calibration is to adjust Robot Arm’s positioning over

mouth A of Reagent Nº1.
Execute the command to initialize the Sample / Reagent Tray and the
commands to initialize vertical and horizontal movement of the Robot Arm.
Then execute the command probeGoReagentA; the Robot Arm will directly
move towards the Sample / Reagent Tray, positioning itself over mouth A of
the container holding Reagent Nº1. The probe must be perfectly aligned
over the mouth. Otherwise, positioning must be corrected simultaneously

on the horizontal movement of the robot arm and on mouth A reagent
offset on the sample / reagent tray.
To adjust horizontal position of the Robot Arm, the commands

obtained position.
To adjust the offset of Reagent mouth A on the Sample / Sample tray, the
commands srMoveBackward or srMoveForward must be ejecutados. Once
the desired postion has been obtained, execute the command
eepromWrite A1 122 XXX where XXX is the new position obtained.
For a better visualization, execute the command probeMoveIn and enter

600 steps as argument. The probe must be inside the reagent container,
centered in relation to the container’s mouth.
If, for any reason, the Sample / Reagent tray has been removed, its position
can be slightly modified during mounting. When adjusting the Allen M5
screws on the del Sample / Reagent tray we will have the backlash. This will
bring about a little side displacement. Because of this, it is important to
check this position before modifying Offset calibration of reagent A
container every time the tray is removed.
Note: Once adjustment has been performed, it is advisable to check

the Sample / Reagent tray’s movement.
Important: A complete turn of the Sample / Reagent tray is equal to 2400

steps . As the Sample / Reagent tray has a capacity for 30 reagent
containers, then the number of steps per container is 80. If the Sample /
Reagent tray is dismounted, it is mandatory to check offsets in the three
positions (mouth A, mouth B and samples).
9. Calibrating reagent A container base of vertical movement
The purpose of this calibration is to adjust the vertical trajectory of the robot
arm on mouth A of the reagent container.
Initialize the sample / reagent tray and the robot arm, both horizontal and
vertical.

Execute the command probeGoReagentA. The Robot Arm will directly
move towards the sample / reagent tray, positioning itself on mouth A of
reagent Nº1 container.
Then, execute the command probeIn, which will produce a movement of

the probe according to a value that has been set in EEPROM. The probe
must be positioned the nearest possible to the bottom of the reagent’s
container without activating the impact detector, so as to work with the
smallest possible dead volume of reagent.
If modification of this value is intended, you can resort to the commands

probeMoveIn and probeMoveOut. Once having obtained the desired
position, execute the command eepromWrite A1 22 XXX where XXX is the
newly obtained position. To check this position, you can put 1ml of liquid in
the reagent cantainer and verify that the informed volume in the
asynchronic response is smaller than that value.

movement.
10. Calibrating robot head position over reagent B
The purpose of this calibration is to adjust the robot Arm’s positioning on

mouth B of Reagent Nº1.
Execute the command to initialize the Sample / Reagent tray and the
commands to initialize vertical and horizontal movement of the Robot Arm.
Then, execute the command probeGoReagentB and srGoReagentB. The

Robot Arm will directly move towards the Sample / Reagent Tray,
positioning itself on mouth B of reagent Nº1 container. The probe must be
perfectly aligned over the mouth. Otherwise, positioning must be corrected
by acting simultaneously on the horizontal movement of the robot arm and
on the offset of reagent mouth B offset of the Sample / reagent tray.
To adjust horizontal position of the Robot Arm, the commands

obtained position.

To adjust reagent mouth B offset on the sample / reagent tray, the
commands srMoveBackward or srMoveForward should be executed. Once
the desired position has been obtained, execute the command
eepromWrite A1 124 XXX where XXX is the newly obtained position.

600 steps as argument. The probe must be positioned inside the reagent
container, centered in relation to the container’s mouth.

the sample / reagent tray’s movement.
Important: the same observations apply as those detailed on point 7 in

relation to checking if the sample / reagent tray is removed.
11. Calibrating reagent B container base of vertical movement
arm on mouth B of the reagent container.
Initialize the sample / reagent tray and the robot arm, both horizontal and
vertical.
Execute the commands probeGoReagentB and srGoReagentB. The Robot

Arm will move directly towards the sample / reagent tray and will be
positioned on mouth B of the reagent Nº1 container.
Then execute the command probeIn, which will produce a movement of

the probe according to the value that was set in EEPROM. The probe must
be the nearest possible to the bottom of the reagent container without
activating the impact detector, so as to work with the smallest dead
volume of reagent.
If you want to modify this value, you can resort to the commands
obtained, execute the command eepromWrite A1 24 XXX where XXX is the
newly obtained position. In order to check this position, you can put 1ml of
liquid in the reagent container and verify that the informed volume in the
asynchronic response is smaller than this value.

movement.
12. Calibrating robot head position over sample tube
The purpose of this calibration is to adjust the positioning of the robot arm
on sample tubes.
Execute the command to start the sample / reagent tray and the
commands to start vertical and horizontal movement of the robot arm.
Then, execute the command probeGoSample and srGoSamplew. The

Robot Arm will move directly towards the sample / reagent tray and will be
positioned on the mouth of sample tube Nº1. The probe should be
perfectly aligned over the mouth. Otherwise, positioning would be
corrected by acting simultaneously on the robot arm’s horizontal
movement and on sample offset on the sample / reagent tray.
To adjust horizontal position of the robot arm, the commands

probeArmMoveClock and probeArmMoveCClock should be executed,
obtained position.
To adjust sample offset on the sample / reagent tray, the commands

srMoveBackward or srMoveForward should be executed. Once the desired
position has been obtained, execute the command eepromWrite A1 116
XXX where XXX is the newly obtained position.

600 steps as argument. The probe must be positioned inside the sample
tube, centered in relation to the tube’s mouth.

the sample / reagent tray’s movement.
Important: the same observations apply as those detailed on point 7 in

relation to checking if the sample / reagent tray is removed.

13. Calibrating sample tubes vertical movement base
arm in sample tubes.
Initialize sample / reagent tray and robot arm, both vertical and horizontal.
Execute the commands probeGoSample and srGoSample. The Robot Arm

will move directly towards the sample / reagent tray and will be positioned
on the mouth of sample tube Nº1.
Then, execute the command probeMoveIn, without any parameter, which
will produce a movement on the probe according to the value that has
been set at EEPROM. The probe must be the nearest possible to the bottom
of the sample tube without activating the impact detector, so as to work
with the smallest possible dead volume of sample.
If you want to modify this value, you can resort to the commands
obtained, execute the command eepromWrite A1 20 XXX where XXX is the
newly obtained position.

movement.
14. Calibrating volume meter of the washing container
Remove the washing container’s cap together with its hoses. Execute the
command vesselStatus and, from the asynchronic response waterVessel,
obtain the value after the letters CW (this value may be positive or
negative). Enter that value in EEPROM by executed eepromWrite A1 336
XXX where XXX is the value that has been read respecting its sign.
Put the cap and the hoses belonging to the washing container on a full
container. Execute the command vesselStatus and, from the asynchronic
response waterVessel, obtain the value below after the letters CW (this
value may again be positive or negative) and substract the value obtained
before, respecting the signs. Enter the result of the substraction in EEPROM
by executing eepromWrite A1 338 YYY where YYY is that result respecting
the sign.

15. Calibrating volume meter in the waste container
Remove the waste container’s cap together with its hoses. Execute the
command vesselStatus and, from the asynchronic response residuesVessel,
obtain the value below the letters CR (this value may be positive 341 XXX
where XXX is the value that has been read respecting its sign.
Put the cap and the hoses belonging to the waste container on a full
container. Execute the command vesselStatus and, from the asynchronic
response residuesVessel, obtain the value below after the letters CR (this
value may again be positive or negative) and substract the value obtained
before, respecting the signs. Enter the result of the substraction in EEPROM
by executing eepromWrite A1 343 YYY where YYY is that result respecting
the sign.
16. Calibrating volume meter in the concentrated waste container
This meter is optional and may not be implemented in hardware.
Remove the concentrated waste container’s cap together with its hoses.
Execute the command vesselStatus and, from the asynchronic response
concentratedResiduesVessel, obtain the value after the letters CC (this
value may be positive or negative) Enter that value in EEPROM by
executing eepromWrite A1 346 XXX where XXX is the value that has been
read respecting its sign.
Put the cap and the hoses belonging to the concentrated waste container
on a full container. Execute the command vesselStatus and, from the
asynchronic response concentratedResiduesVessel, obtain the value after
the letters CC (this value may again be positive or negative) and substract
the value obtained before, respecting the signs. Enter the result of the
substraction in EEPROM by executing eepromWrite A1 348 YYY where YYY is
that result respecting the sign.

3.4 Changing the lamp
a. Keeping the equipment off, remove the right upper lid by removing
the screws on the back of the autoanalyzer.
b. Remove the dissipator by manually removing the two screws.
Screws to
remove
c. Disconnect the lamp from the air connector by loosening the allen
screw holding the lamp, so as to be able to completely remove the
lamp to be replaced.
Lamp
Air
Connector

d. Fit in the new lamp. Important: the lamp’s filament must be in
horizontal position. Slightly tighten the Allen screw, then adjust the
focal distance. Connect the lamp to the aerial connector.
e. Switch on the equipment. From the Main Menu, go to Maintenance
Maintenance - Communications – Command Console. Start the
photometer and select a visible range wave length (546 nm)
f. Remove the cuvette’s strip from the optical path, place a piece of
white paper to check the light beam’s clarity on it. If it were
necessary, adjust focal distance by moving the lamp, without
rotating it – so as to keep the filament in its horizontal position-. You
should aim at focusing the beam on the center of the cuvette’s
optical way. Once adjustment has been achieved, fix the lamp.
g. Calibrate the photometer and the 100 cuvettes from the
Maintenance – Equipment – Calibration screen.
3.5 Changing interferential filters
a. With the equipment off, remove the upper and side lids of the
autoanalyzer to have access to the photometer, located on the right
back side.
b. Remove the photometer’s upper lid.
c. Spot the interferential filter to be replaced and manually turn the
filter’s wheel clockwisely so as to position the interferential filter and
easily remove it from the filter wheel.
d. Dismount the filter’s holder manually, remove the filter and replace by
a new filter.
Filter
holder

Filter
holder
e. Replace the lids, switch on the equipment.

f. Calibrate the photometer from the Maintenance screen,
“Photometer calibration” and select all cuvettes.
3.6 Changing the Sample / Reagent probe

a. Keep the equipment off, remove the blue lid on the Sample /
Reagent arm.
b. Disconnect the teflon tubing from the hydraulic connector.
Teflon
tubing
Fixing
screws

c. Disconnect the connector from PCB and remove the fixing screws on
the PCB. Remove the PCB so as to be able to completely remove the
Sample / Reagent probe.
d. Replace the Sample / Reagent probe. The new probe has a

polarizing pin to make mounting easier.

Pin
e. Mount the PCB and connect the teflon tubing.

f. Perform several priming cycles and check that there is no liquid leak.
3.7 Changing the TECAN syringe

a. On the main screen, go to Maintenance – Communications –
Command Console, to start the syringe.

Fixing area of
the syringe to the
valve
Fixing
screw
b. Once the syringe has been started, execute the command

syringeAspirate, entering the value100 in “parameter”. Once the
piston has moved, proceed to dismount.
c. Remove the fixing screw completely. This screw is placed at the end
of the piston. Turn the syringe body upwards so as to completely
dismount it from the dilutor holder.
d. Install the new TECAN syringe. Once it has been placed, fix by
means of the fixing screw, but not exerting excessive force (DO NOT
use any tools) so as to avoid breaking the syringe.
e. Perform several priming cycles from the “Maintenance” screen.
3.8 Changing tubings
Changing the hydraulic circuit tubings.
a. Replace tubings according to the kind of material and length.

b. Perform several priming cycles so as to check that there are no leaks on
the hydraulic connectors.

Changing Peristaltic Pump tubing
Hydraulic
Connector
Hydraulic
Connector
a. Lift the acrylic lid, push the left white lever to the center of the
peristaltic pump and pull up the tubing.
b. Remove the tubing, turn the peristaltic pump rollers manually so as to

release the pump.

c. Push the right white lever towards the center of the peristaltic pump in
order to free the other extreme of the tubing from its holder.
d. Place the new tubing on the peristaltic pump. Start on the left
extreme. Hold it shut with the white lever, pass it through the rollers,
manually turn the rollers and, finally, fix on the right side by means of
the white lever.
e. Connect both extremes of the tubing to the hydraulic connector.
f. Perform primes of the dilutor until the hydraulic circuit has been
refilled.
3.9 Changing the cuvettes

a. Every cuvette strip is held by two screws. Remove the strips one by
one.
b. Place the new cuvette strips.
c. Calibrate cuvettes from the Maintenance screen, Maintenance –
Equipment – Calibration, selecting all cuvettes.

3.10 Obtaining parameters from firmware values.
In order to obtain module parameters of factory adjustments, you must
enter the Maintenance screen, select “Configuration” and, finally,
“Memory”.
All parameters will be displayed on the screen with their blank fields, so as to
be able to obtain all Sapphire autoanalyzer’s parameters. Press the button
Obtain from the instrument. We will be able to check the percentage of
information transference on the progress bar. Once the transference has
finished, all fields will have been filled with the factory adjustment values.
To visualize the other parameters, you will only need to select the button of
the module to be checked. For example, Washer, direct the pointer there,
press that button and you will have all parameters with their adjustment
values on the screen.
Note: it is highly advisable to keep a copy of the equipment parameters

together with the Sapphire autoanalyzer’s installation report.
4 Maintenance program.

In order to achieve optimal perfomance, as well as a longer useful life
for the Autoanalyzer, it is important to follow the instructions for its
maintenance and cleaning.
4.1 Daily Maintenance

• Inspect the waste and washing solution containers to make sure
they have the required level to start daily work.
• Inspect the reagent container and sample tubes to make sure
they have the required level to start daily work.
• Prime the dilutor (do not allow the dilutor to perate without water
any more than a few cycles) before performing a run.
• Wash the 10 first cuvettes (scope 1 to 10) before starting daily
operation.
• Clean the tip thoroughly, by using commercial sodium
hypochlorite, in the selected tube at the end of the day’s work.
• Wash all dirty cuvettes before finishing the day’s work.
4.2 Weekly maintenance

• Calibrate the photometer and cuvettes.
4.3 Six-monthly maintenance

• Replace peristaltic pump tubing (or else, every 3.000
determinations)
• Check unidirectional valves (check valve) and washing station
tips.
Note: DO NOT clean washer’s tips with any kind of alcohol. Only
use diluted sodium hypochlorite (commercial kind), rinse
several times with distilled water.
• Decontamination and general cleaning of the instrument.

• Change of the TECAN syringe (or else every 180.000
determinations).
• Lamp change

5 Troubleshootings.
This Troubleshooting section provides help to solve situations the Sapphire
autoanalyzer operator may encounter.
For any problem other than those described here, contact exclusively the
Technical Service authorized by Audit Diagnostic.
Problems may be gathered in three main groups:
1. Malfunction problems, on display on the screen.

2. Visible flaws
3. Inconsistent measurements
5.1 Malfunction problems displayed on the screen

If an error message warns us about the existence of a mechanical flaw, in
order to find out which module is generating this mechanical flaw, we
should go to the Log file. To access this file we should go to the
“Maintenance” screen, then “Configuration” and find the path for the Log
file.
Once the path has been found, minimize the program Sapphire. Search for
the Windows User folder (or the one in that path) in the local disk. Once the

folder has been found, search the screen for log.html files. These are
identified by day of the week, month, day and year (for example, the file
corresponding to Tuesday, April 6th , 2004 will have the name Tue apr 6
2004.log.html).
Once the file has been found, position yourself on it and double click to
open the file. It will be opened by Windows Explorer.
In order to locate the error message shown on the screen, it is convenient to

start by the last transactions on the Serial port. We can even check if the
same error has been repeated in previous transactions. The message
informs us about the moment the error was produced, the process number
and the kind of error. All this information will enable us to find it on the
screen.
We can also search for errors on the basis of Sapphire autoanalyzer`s

response (Serial In) to computer (PC). This search is done by Edition – Search
this page (or else by ctrl+F). The number code or the complete text of the
command is entered and the searcher will start the search.
5.2 Log.html
This file is a powerful tool for error search, as well as for an accurate
diagnosis when facing systematic or random flaws.
All bidirectional messages for all the commands required by the computer
(PC) and executed by Sapphire autoanalyzer, and all their responses, will
be found in log.html.
Let us analyze the following message structure obtained from the log.html
file. The computer (PC) sends a command to initialize vertical movement of
Sapphire, autoanalyzer’s Robot. We will have the following message
structure in log.html:
16:28:32.171 - Serial Out: probeInit A1

16:28:32.186 - Serial In: status A1 PC OK *#I-49,0 @00:12:15.808
16:28:32.639 - Serial In: probeCurPos A1 PC 0 *E0 iHl #O-49 @00:12:16.203
16:28:32.171 Serial Out is the command sent by the computer (PC) to

Sapphire autoanalyzer number one (A1) to initialize the Robot’s vertical
movement.
16:28:32.186 Serial In is the command sent by Sapphire autoanalyzer

number one (A1) to the computer (PC) to be executed. Send the following

response message, status OK no comments *, #I-49 which is the order
number given to entries by the controller’s meter. Finally, the time when the
command was executed.
16:28:32.639 asynchronic response to the command required and executed

by Sapphire autoanalyzer.
Once the Robot’s vertical movement has been initialized, Sapphire
autoanalyzer sends the computer (PC) the asynchronic response to the
required command. This is an indication that it has been positioned in
position 0 in relation to the Home sensor and there is no error E0. It is also
showing the state of sensors iHl; after that, the order number given to the
exit by meter #O-49and, finally, the time the command was executed.
Sensor state is very useful in order to establish a correlation with regard to

mechanical movements. The first letter, for example, tells us about the
abnormal descent sensor on the Sample/ Reagent probe. If there was a
collision, letter i becomes I (capital letter). By H it is indicating that the Home
sensor is activated. When it is deactivated, it becomes h. By l (small letter
el) it indicates that the lever detector is deactivated. When it is detecting
liquid, it becomes L.
When one or several commands are queued and an error appears, these
commands are eliminated right away. Therefore, there is no response for
those commands. Let us look at the following log.html of Sapphire
autoanalyzer’s starting sequence. The error message generates a Stop
alarm and is immediately shown by means of a message in the user’s
interface.
14:36:28.889 - Serial Out: flushAll A1

14:36:28.905 - Serial Out: setQueue A1 0
14:36:28.920 - Serial Out: photoInit A1
14:36:28.936 - Serial In: queue A1 PC 0 *#O-12 @00:01:09.274
14:36:28.952 - Serial Out: probeInit A1
14:36:28.998 - Serial Out: probeArmInit A1
14:36:29.014 - Serial In: status A1 PC QUEUED *#I-15,0 @00:01:09.354
14:36:29.014 - Serial Out: probeGoFunnel A1
14:36:29.045 - Serial Out: srInit A1
14:36:29.061 - Serial Out: srGoReagentA A1 1

14:36:29.077 - Serial Out: syringeInit A1
14:36:29.092 - Serial Out: washerInit A1
14:36:29.108 - Serial Out: valveSet A1 probe
14:36:29.139 - Serial Out: reactionInit A1
14:36:29.436 - Serial In: probeCurPos A1 PC 0 *E0 iHl #O-14 @00:01:09.741
14:36:30.420 - Serial In: probeArmCurPos A1 PC 0 *E0 iH #O-15 @00:01:10.649
14:36:30.420 - Serial In: probeArmCurVal A1 PC Funnel @00:01:10.662
14:36:30.420 - Serial In: probeArmCurPos A1 PC 0 *E0 iH #O-16 @00:01:10.688
14:36:30.420 - Serial In: probeArmCurVal A1 PC Funnel @00:01:10.700
14:36:31.952 - Serial In: photoCurVal A1 PC 340 *P54 *E0 H #O-13
@00:01:12.235
14:36:33.373 - Serial In: srCurPos A1 PC 352 *E0 iHC #O-17 @00:01:13.650
14:36:33.373 - Serial In: srCurVal A1 PC ReagentA 1 @00:01:13.662
14:36:33.373 - Serial In: srCurPos A1 PC 352 *E0 iHC #O-18 @00:01:13.689
14:36:33.373 - Serial In: srCurVal A1 PC ReagentA 1 @00:01:13.702
14:36:33.592 - Serial In: washerCurVal A1 PC !error *P0 *E1 UD #O-20
@00:01:13.922
14:36:35.123 - Serial In: syringeCurPos A1 PC 0.000 *` #O-19 @00:01:15.443
14:36:35.123 - Serial In: valveCurVal A1 PC Probe *` #O-19 @00:01:15.457
According to the log.html the cuvette washer showed an error (see text in
blue letters). The computer sends a command to initialize the cuvette
washer washerInit. Sapphire autoanalyzer receives the command and
places it on the execution queue. It continues executing the other required
commands, until it receives the asynchronic response with error
washerCurVal, corresponding to the washerInit command required by the
computer (PC). We find that position sensors up&down of the washer are
both active at the same time. Let us observe that the subsequent
commandos (#21 y #22) to the one producing the error (#20) were not
executed, but #19 was completely executed, even after the error was
detected.
Detector Status and level sensor table

Module Command Status Description
Robot Vertical Position probeInit iHl Initializing vertical Mov.
Robot Horizontal Position probeArmInit iH Initializing horizontal Mov.
Reaction Tray ReactionInit ihw Initializing Reaction Tray
Sample-Reagent Tray srInit iH Initializing S-R Tray

Photometer Filter Wheel photoInit H Filter position synchronism
Washer Initialize Washer washerInit Ud Initializing washer
Activated Deact. Comment

Probe Impact I i I: probe impact, Stop alarm
Starting Position H h H: detected starting position
S/R Sensor L l L: detected sample and/or
reagent level
Cuvette Washing W w W: the washer is not up
Filter Position H h H: filter position synchronism
Pos. Washer U u U: washer outside cuvettes
Pos.Washer down D d D: cuvette washer down
Let us analize a Mechanical Error message:
We search the log.html for possible failures.

By looking at log.html we can notice that one of the possible failures is the
Robot. When we initialize the arm we get the !error answer. The comment is
*P300 *E1 and the sensor state indicates that impact sensor, IHl is activated.
When there is no error, the letter is E=0. Letter P indicates the number of
steps the mechanism has performed. The following is an error list that may
appear in Sapphire autoanalyzer’s responses and their meaning:
E1: movement cannot be initialized.
E2: probe impact
E3: home sensor not found
E4: movement verification error
E5: checking sensor not found
E6: level sensor does not activate
E7: washer is not up
E8: stopped due to lack of movement safety conditions
“debug” messages may appear, which require additional information. They

may be sent either from the computer (PC) or from Sapphire autoanalyzer
It is important to bear in mind the asynchronic response table (see Section
7.5 of the Technical Service Manual) to be able to interpret the responses
sent by Sapphire autoanalyzer to the computer (PC).
5.3 Fast Test

The different modules can be quickly verified and Serial Port log can be
visualized in the Command Console at the same time, by means of this test.
By means of the Command Console we can execute commands to
initialize, enter parameters to execute movements, activate or deactivate
the lamp, check heater or preheater temperature, or execute other
commands. Just select the command, give the parameter (if
necessary)and, finally, execute.
To enter this test, go to the Maintenance screen, access Communications
and the Commands Console. The following image will be displayed on the
User’s screen:

5.4 Visible Flaws
Cause Action to take

Drops after dispensing Clean tip
Bubbles in hydraulic system tubings Adjust connections and prime dilutor
5 times
Drops in dilutor syringe Replace syringe
Drops in peristaltic pump hose Change hose and adjust extremes
correctly
Drops after internal tubing washing Adjust connections and verify
possible obstructions in the hydraulic
system. Prime dilutor afterwards
Abnormal noises Faulty fan operation. Movable parts
Blocked or touching. Contact
technical service

5.5 Inconsistent Measurements
Inconsistent measurements have different origins and they will be grouped

as:
• Inaccurate results in all analytes
• Inaccurate results in some analytes
• Inaccurate results due to faulty reagents or controls.
5.6 Inaccurate results in all analytes

This may have various origins: dilutor’s hydraulic system, cuvette washer,
cuvettes and photometer.
a) Originated in the dilutor’s hydraulic system
b) Originated in the cuvette washer
c) Originated in the cuvettes and photometer
It is obviously essential to diagnose the inaccuracy origin. To do so, we have

the instrument’s validation tests in the “Maintenance” menu. They will help
us diagnose the origin.
a) Originated in the dilutor’s hydraulic system
Cause Action
Dirty hydraulic system Tip cleaning
Bubbles in hydraulic system tubings Adjust connections and first dilutor 5
times
Dripping in the dilutor’s syringe Replace the syringe
b) Originated in the cuvette washer
Cause Action
Steel tubings obstructed Eliminate obstruction
c) Originated by photometer and cuvettes
Cause Action

Damaged cuvettes Change cuvette and calibrate
optical system
Faulty lamp Change lamp and calibrate optical
system
5.7 Inaccurate results in some of the analytes

Inaccurate results only in some of the analytes can only have a few
origins.
Kinetic and fixed-point kinetic methods
Kind of results Possible cause Action to take

High control High heater and Check
Values preheater temperature from
temperature command console.
Contact technical
service
Low control Low or non Check temperature
values stabilized from command
preheater and console. Contact
heater technical service
temperature.
Low control Deteriorated filter Contact technical
values service
Erratic values Insufficient sample Adapt method
or reagent volume correctly
Final point methods

Kind of results Possible cause Action
Erratic values Insufficient reagent Adapt method
or sample volume correctly
Erratic values Deteriorated or low Calibrate optical
energy filter. system. Contact
technical service
Erratic Values Short incubation Adapt method
time correctly
5.8 Inaccurate results due to faulty reagents, controls or samples
Reagents in a bad state, calibrators and controls in a bad state, or

badly kept, will provide inaccurate and inconsistent results.
Hemolized, icteric or murky samples, or the use of plasma may not
be advisable for use in some methods.
5.9 Comunication Protocol for Technical Service use
Introduction
The autoanalyzer will be communicated to the PC through a serial port

RS232C by means of bidirectional messages whose structure is described as
follows:
Message Structure
Commands between the PC and the autoanalyzer will have the following
structure:
{Command} {SP} {For} [{SP} {Argument}] {\r}
where the field {Command} is described here: {SP} is the space character
(ASCII 32), {For} is the destination name for the message (for ex: A1, A2,
etc.), {Argument} is an optional field that depends upon the command and
{\r} is the return character (ASCII 13) and corresponds to the end of the
message.
Execution of a command starts when the PC sends a message to the

Autoanalyzer corresponding to a command (the command list is described
in Command Description). The autoanalyzer responds by sending a
response message where it reports the processing given to the received
command, as described in Response Description. If the command requires
transmitting complementary information to the PC, it will afterwards send an
asynchronic response, as described in Asynchronic response Description.
Command Description
The following is a description of the commands, grouped by module of the

autoanalyzer.
Sample Probe
Description Command Alias Argument
Initialize vertical movement probeInit pbI No
Searching upper sensor probeOut pbOut No
Searching level sensor probeIn pbIn No

Decrease steps probeMoveIn pbMI uint steps
(optional)
Decrease steps not detecting probeMoveInWithoutLevel pbMIWL uint steps
level (optional)
Increase steps probeMoveOut pbMO uint steps
Starting horizontal movement probeArmInit pbAI No
Move steps to the left probeArmMoveClock pbAMC uint steps
Move steps to the right probeArmMoveCclock pbAMCc uint steps
Position on cuvettes for probeGoDispense pbGD No
dispensing
Position on washing funnel probeGoFunnel pbGF No
Position on sample probeGoSample pbGS No
Position on reagent A probeGoReagentA pbGRA No
Position on reagent B probeGoReagentB pbGRB No
Reaction Tray
Initialize reaction tray reactionInit rI No
Cuvette to dispensing position reactionGoDispense rGD uint cuvette
Cuvette to photometer reactionGoPhoto rGP uint cuvette
Cuvettes forwards reactionForward rF uint cuvette
Cuvettes backwards reactionBackward rB uint cuvette
Advance steps reactionMoveForward rMF uint steps
Steps backwards reactionMoveBackward rMB uint steps
Sample-Reagent Tray
Initialize S-R Tray srInit srI No
Go to sample srGoSample srGS uint sample
Go to reagent A srGoReagentA srGRA uint reagent
Go to reagent B srGoReagentB srGRB uint reagent
Advance steps srMoveForward srMF int steps
Backward Steps srMoveBackward srMB int steps
Pump
Initialize pump pumpInit pmI int steps
Advance steps pumpMoveForward pmMF int steps
Backward Steps pumpMoveBackward pmMB int steps
Pump pulse pumpPulse pmP int pulses

Heater
Set temperature heaterSet hS float Temp
Switch on heater heaterPowerOn hPOn No
Switch off heater heaterPowerOff hPOff No
Read temperature heaterRead hR No
Preheater
Set temperature preheaterSet prS float Temp
Switch on preheater preheaterPowerOn prPOn No
Switch off preheater preheaterPowerOff prPOff No
Read temperature preheaterRead prR No
Photometer
Initialization photoInit phI No
Go to filter photoSetFilter phSF int lambda
Read photoRead phR No
Switch on lamp lampHigh lH No
Switch off lamp lampLow lL No
Washer
Initialize washer washerInit wI No
Down washerIn wIn No
Up washerOut wOut No
Fill washerFill wFi int mseg
Empty washerFlush wF int mseg
Empty reaction washerFlushReaction wFR int mseg
Switch on vacuum pump washerVacuumOn wVon No
Switch off vacuum pump washerVacuumOff wVOff No
Dilutor
Initialize syringe syringeInit sI No
Collect (relative)volume syringeAspirate sA float volume
uint veloc
Dispense(relative)volume syringeDispense sD float volume
uint veloc
Fix (absolute) volume syringeSet sS float volume
uint veloc

Start valve valveInit vI No
Move valve to pump or valveSet vS Probe / Pump
syringe / ByPass
Miscelaneous
Select queue setQueue -- uint queue
Delete all queues flushAll -- No
Erase selected queue flush -- No
Calibrate photometer calibratePhoto cP No
Calibrate cuvette calibrateCuvette cC No
Read container level vesselStatus -- No
Delete EEPROM eepromClear -- No
Erase cuvette absorbances eepromClearAbs -- No
from EEPROM
Type EEPROM eepromWrite -- uint Address
{byte/int/
uint/long/
float/string}
Data
Read EEPROM eepromRead -- uint Address
Type a byte for EEPROM eepromWriteByte -- uint Address
Read a byte from EEPROM eepromReadByte -- uint Address
Type EEPROM version eepromVersionWrite -- uint Address
Read EEPROM version eepromVersionRead -- uint Address
Wait Delay -- uint mseg
Send a non executable debugMessage -- Unspecified
message
The kinds of variable are:

byte: integer, 8 bits, no sign.
int: integer, 16 bits with a sign.
uint: integer, 16 bits no sign.
long: integer, 32 bits no sign.
float: floating point.
string: character chain.
A command for lowering the probe until detecting level would be:
probeIn A1\r
Response Description

Once it has received the command, the Autoanalyzer sends a response
message to the PC. The word indicating that it is a response is status and the
argument will reflect the result of the processing performed by the
autoanalyzer’s main controller with the command. Possible arguments are:
Argument Description
OK The command was correctly received and will be
executed
SYNTAX the command received has a syntax mistake and must
be resent by the PC
BUSY The Autoanalyzer is busy and cannot execute the
command received, which must be resent by the PC
QUEUED The command received cannot be immediately
executed by the Autoanalyzer, but has been placed in
the queue
UNKNOWN the command received is unknown by the Autoanalyzer
The Autoanalyzer’s response to the command in the previous section

would be:
status A1 PC OK\r
Asynchronic Response Description

If the commands involve a change in the Autoanalyzer’s state, (for ex. Tray
or arm movements, temperature reading, etc.) the Autoanalyzer will notify
the PC in an asynchronic way once the command has finished. This notice
will exist, no matter whether the change of state is actually produced (for
ex.: move in the tray to the position where it is). If the change of state
affects several variables, the Autoanalyzer will send the PC an asynchronic
response for each affected variable (for ex.: the command
reactionMoveBackward will generate the responses reactionCurPos,
reactionCurDispense and reactionCurPhoto). In these cases, the execution
time will only be sent in the first response . The following are the responses
corresponding to the commands involving changes in the Autoanalyzer’s
state:
Commands Response Argument

probeInit probeCurPos int steps
probeOut
probeMoveIn
probeMoveInWithoutLevel
probeMoveOut
probeIn probeCurPos int steps

reagentAsingleCurVol (2) float mL
reagentAdoubleCurVol (2) float mL
reagentBCurVol (3) float mL
probeArmInit probeArmCurPos int steps
probeArmMoveClock probeArmCurVal Cuvette /
probeArmMoveCclock Funnel /
probeGoDispense Sample /
probeGoFunnel ReagentA /
probeGoSample ReagentB / None
probeGoReagentA
probeGoReagentB
reactionInit reactionCurPos int steps
reactionGoDispense reactionCurDispense int cuvette / 0
reactionGoPhoto reactionCurPhoto int cuvette / 0
reactionForward
reactionBackward
reactionMoveForward
reactionMoveBackward
srInit srCurPos int steps
srGoSample srCurVal Sample /
srGoReagentA ReagentA /
srGoReagentB ReagentB / None
srMoveForward int position / 0
srMoveBackward
pumpMoveForward pumpRelMove int steps
pumpMoveBackward
pumpPulse
heaterRead heaterCurVal float Temp
heaterSet heaterCurSet float Temp
heaterPowerOn heater On / Off
heaterPowerOff
preheaterRead preheaterCurVal float Temp
preheaterSet preheaterCurSet float Temp
preheaterPowerOn preheater On / Off
preheaterPowerOff
photoInit photoCurVal Black / int
photoSetFilter lambda / None
photoRead photoCurRead long int sample
long int
reference
lampHigh lamp High / Low
lampLow

washerInit washerCurVal Out / In / None
washerOut
washerIn
washerFill washerFilled 0% / 100%
washerFlush
washerFlushReaction
washerVacuumOn washerVacuum On / Off
washerVacuumOff
syringeInit syringeCurPos float volume
valveInit valveCurVal Probe / Pump /
ByPass
syringeAspirate syringeCurPos float volume
syringeDispense
syringeSet
valveSet valveCurVal Probe / Pump /
ByPass
setQueue queue uint queue
calibratePhoto photoCalibration OK
calibrateCuvette cuvetteCalibration OK
vesselStatus waterVessel XX%
residuesVessel
concentratedResiduesVessel
eepromClear eepromClear OK
eepromClearAbs eepromClearAbs OK
eepromWrite eepromWrite OK
eepromWriteByte
eepromRead eepromRead {byte/int/uint/
eepromReadByte long/float} Data
eepromVersionWrite eepromVersionWrite OK
eepromVersionRead eepromVersion string EEPROM
version
setQueue queue uint queue
(2) Response sent when the arm is positioned over reagent mouth A.
(3) Response sent when the arm is positioned over reagent mouth B.
After the arguments described, there can be a comment field that will be
identified by the character ‘*’ (asterix: ASCII 42).
Finally, the autoanalyzer will inform the PC about the time when the
required command was completed (measured from the moment the
equipment was switched on). This field will be identified by the character ‘@’

(ASCII 64) and it will have the format h:m:s where h is the time, m is the
minutes and s is the seconds passed (expressed with three decimals).
If it were necessary, the Autoanalyzer could send “debug” messages by

means of the debugMessage response, which will be added after the
asynchronic response requiring additional information.
There may happen that the PC starts sending a command while the
Autoanalizador is delivering an asynchronic response. In that case, the
Autoanalyzer will finish sending the asynchronic response and it will deliver
the status message afterwards.
5.10 EEPROM version 1.02 directions

Main Controller
Variable Direction Type
Serial number 1 string
EEprom version 8176 string
Sample Probe Vertical Movement

Cuvette Base 16 int
Funnel Base 18 int
Sample tube Base 20 int
Reagent A container Base 22 int
Reagent B flask Base 24 int
Starting Frequency 26 int
Maximum Frequency 28 int
Step Repetition 30 byte
Sample Probe Horizontal Movement

Dispensing 48 int
Funnel 50 int
Samples 52 int
Reagent A 54 int
Reagent B 56 int
Starting frequency 58 int
Maximum frequency 60 int
Step repetition 62 byte
Reaction Tray

Steps per cuvette 80 int
Cuvette 1 dispensing Offset 82 int
Cuvette 1 photometer Offset 84 int
Sample-Reagent Tray
Number of sample tubes 112 int
Steps per sample tube 114 int
Sample tube 1 Offset 116 int
Number of reagent flasks 118 int
Steps per reagent flask 120 int
Reagent A 1 Offset 122 int
Reagent B 1 Offset 124 int
Filter Wheel
Steps per filter 144 float
Offset filter 1 148 int
Reading Delay 150 int
Optical path 152 float
Sample channel reading threshold 161 long
Reference channel reading threshold 164 long
Dilutor
Total volume steps 176 int
Syringe Volume 178 int
Ramp 184 byte

Pump
Pump steps per pulse 213 int
Heater
Proportional profit 240 int
Integral profit 242 int
Derivative profit 244 int
Oversampling 246 byte
Automatic switching 247 byte
Proportional limit 248 int
Integral limit 250 int
Derivative limit 252 int
Temperature 254 float
Temperature Offset 256 float
Preheater
Proportional profit 272 int
Integral profit 274 int
Derivative profit 276 int
Oversampling 278 byte
Automatic switching 279 byte
Proportional limit 280 int
Integral limit 282 int
Derivative limit 284 int
Temperature 286 float
Temperature Offset 288 float
Washer
Filling Time 304 int
Emptying Time 306 int
Emptying Prime Time 308 int
Starting Frequency 310 int
Maximum Frequency 312 int
Cuvette Base 315 byte

Containers
Zero washing container 336 int
Maximum washing container 338 int
Washing container volume 340 byte
Zero diluted waste container 341 int
Maximum diluted waste container 343 int
Diluted waste container volume 345 byte
Zero pure waste container 346 int
Maximum pure waste container 348 int
Pure waste container volume 350 byte
Filters
Filter 1 2 3 4 5 6 7 8 9 10 11 12
Wavelength 512 544 576 608 640 672 704 736 768 800 832 864 int
Sample profit 514 546 578 610 642 674 706 738 770 802 834 866 byte
Ref. profit 515 547 579 611 643 675 707 739 771 803 835 867 byte
Log I0 516 548 580 612 644 676 708 740 772 804 836 868 float
Sample white 520 552 584 616 648 680 712 744 776 808 840 872 long
Ref. white 523 555 587 619 651 683 715 747 779 811 843 875 long
Zero Sample 526 558 590 622 654 686 718 750 782 814 846 878 long
Zero Ref. 529 561 593 625 657 689 721 753 785 817 849 881 long
Cuvette absorbances
Filter 1 2 3 4 5 6 7 8 9 10 11 12
Cub. 1 2048 2052 2056 2060 2064 2068 2072 2076 2080 2084 2088 2092 float
Cub. 2 2100 2104 2108 2112 2116 2120 2124 2128 2132 2136 2140 2144 float
Cub. 100 7196 7200 7204 7208 7212 7216 7220 7224 7228 7232 7236 7240 float
The formula to calculate the corresponding EEPROM direction for a

cuvette’s absorbance at a certain wavelength is:
(CUVETTE NUMBER - 1) * 4 * 13 + (FILTER NUMBER - 1) * 4 + 2048

6 Validation Program
6.1 Photometrical Parameters to Control

The aim of this document is to verify if photometrical parameters are within
the acceptability scope of international regulations.
In order to do so, the following set of solutions, elaborated with analytical
quality drugs according to international regulations, will be used. Controls
and certification of these solutions were made against Reference Standards
certified by the NIST (National Institute of Standards & Technology)
1.Spurious light:
- Sodium Nitrite Solution 50 g/l
2.Photometrical Accuracy:
- To evaluate UV (340 nm):
• 2 Potassium Dichromate solutions in HClO4 0.001 N
- To evaluate Visible (510 nm):
• 2 Cobalt Sulphate solutions in SO4H2 0.45 N
3. Photometrical Linearity:
- 340 nm: 4 different concentration solutions of Potassium Dichromate:
A1, A2, A3, A4.
- 405 nm: 4 different concentration solutions of p-nitrophenol: C1, C2,
C3, C4.
- 510 nm: 4 different concentration solutions of Cobalt Sulphate: B1,
B2, B3, B4.
4.Photometrical accuracy :
- Pentahydrated Copper Sulphate solution in SO4H2
NOTE: electronic system must be calibrated before performing the

tests. Remember to use new cuvettes and 37 °C temperature.
6.2 Spurious light

Definition: The name spurious or parasite light is given to any
electromagnetic radiation reaching the detector which has a different
wavelength to the selected one and, therefore, is recorded by the
instrument
Basics: this control is based on the measurement of the transmittance
percentage of a sodium nitrite solution. This substance has the property
that its solutions absorb all striking radiation of wavelength smaller than
390 nm. That is why it is called optimally opaque to light. Therefore, this
solution’s transmittance at 340 nm should be equal to zero, and all
detected transmittance corresponds to parasite light.
Materials needed:
50 g/l solution of NaNO2
Procedure:
• Working temperature: 37 °C
• The solution’s transmittance is independent from temperature
between 10 and 40 ºC
• Wavelength: 340 nm
• Prepare a white with distilled water. Perform duplicate
measurements.
Results:
Percentage transmittance value is defined as the spurious light present

in the spectral zone.
Spurious light = TRANSMITTANCE % = 10 (2 – ABSORBANCE)
Example:
Absorbance = 2.300 UA corresponds to T % = 0.5 %
Acceptability limits:
Optimal spurious light: T% lower than 0.5 %

(abs. higher than 2.300 UA).
Acceptable spurious light :T% lower than 1 %

(abs. higher than a 2.000 UA)
6.3 Photometrical Stability
Definition of Photometrical Stability:

Basics:
Materials needed:
Potassium dichromate solution in H ClO4 0.001N with absorbance = 2
Procedure:
• Working temperature: 37 °C
• Wavelength: 340 nm
• Perform a white with distilled water.
Pour a solution of 2,0 of A at 340 nm and measure for 30 min (1 reading

per minute). Observe how measurements are repeated.
Results:
The corresponding statistical calculation will be made, thus obtaining

standard deviation as electronic noise.
Photometrical stability = SD (30 absorbances read)
Optimal photometrical stability: 0.001 UA a 2,0 UA

Acceptable photometrical stability: < o = 0.002 UA a 2,0 de UA

6.4 Photometrical Accuracy
Definitions:
Photometrical accuracy is the degree of consistency between real
absorbance and measured absorbance. The mistake made when
reading absorbance in relation to a reference one is then called
“photometrical inaccuracy”.
Basics:
The study of photometrical inaccuracy consists of measuring
absorbances of certified solutions and compare the values found with
reference ones.
Materials needed:
-To evaluate UV (340 nm):
• 2 Potassium dichromate solutions in H ClO4 0.001N
-Para evaluar Visible (510 nm):

• 2 Cobalt Sulphate solutions in SO4H2 0.45 N
Procedure:
Absorbance of the solutions should be measured and such value should
be compared to the values in the corresponding certificates.
Write down working temperature.

Select wavelength:
• 340 nm for Potassium Dichromate solutions in HClO4 0.001 N.
• 505 nm for Cobalt Sulphate solutions in SO4H2 0.45 N.
Make a white with distilled water. Duplicate all measurements.
Results
Calculate photometrical inaccuracy as the difference between the
average absorbance found and the reference value shown in the
corresponding certificates.
% photometrical inaccuracy = (Abs. found – Reference Abs.) x 100

Reference Abs.
Optimal Photometrical Accuracy: error between +/- 2 %
Acceptable Photometrical Accuracy: error between +/- 3 %
Example:

- Wavelength: 505 nm
- Temperature: 37 ºC
- Absorbance against distilled water of Cobalt Sulphate solution in
SO4H2 0.45 N:
Measurement 1: 0.315 Measurement 2: 0.313
- Average absorbance found : 0.314
- Reference absorbance, 37 ºC, 505 nm (according to certification):
0.323
- Photometrical Inaccuracy = (0.314 – 0.323) x 100 = - 2.78 %
0.323
Therefore, the instrument’s inaccuracy at that wavelength is within

acceptable limits.
6.5 Photometrical Precision
Definition: The name Photometrical Precision is given to the

measurement of the dispersion of a series of transmittance or
absorbance measurements around the mean. It is expressed as a
coefficient of percentage variation.
Materials needed:
Copper Sulphate Solution in sulphuric acid.
Procedure:
- Wavelength: 650 nm
- Working temperature: 37°C
Load a reaction cuvette with water, where the white will be read.
Load another cuvette with the solution and read absorbance 10
times.
NOTE: this solution’s absolute absorbance is not important, since it is
not a reference solution to determine absorbance accuracy .
Therefore, reference absorbance is not stated. Do not mind any
Copper Sulphate precipitate that may appear on the lid. Only the

percentage variation coefficient obtained as an indicator of
inaccurate readings is important.
Results:
10 absorbance results will be obtained.
Calculate ARYTHMETIC MEAN X and standard deviation SD of those

absorbances.
Finally, obtain the PERCENTAGE VARIATION COEFFICIENT:
Photometrical Inaccuracy = CV% = (SD / X) × 100
Optimal accuracy: CV% lower than 0.5 %

Acceptable accuracy: CV% lower than 1 %
Example: The following readings were obtained from Copper Sulphate

at 650nm:
0.224, 0.224, 0.225, 0.223, 0.225, 0.226, 0.223, 0.224, 0.227, 0.222.
The mean of the 10 measurements is: X = 0.224
Standard deviation will be: SD = 0.0015
Therefore, CV% will be:
CV% = (0.0015/0.224) x 100 = 0.67%, which exceeds optimal limit but is still
acceptable.
6.6 Photometrical Linearity

Definitions:
Linear response capacity of an spectrophotometer at different
concentrations of a substance complying with Beer’s Law.
Basics:
Photometrical linearity study allows us to establish the scope of
absorbance where the instrument has responses that are proportional to
concentration changes.
Procedure:
Solution absorbances should be measured and such values be
contrasted with reference ones on the corresponding certificates.
Write down the working temperature.

Select wavelength:
• 340 nm (solutions A1, A2, A3, A4)
• 500 nm, 505 nm or 510 nm (solutions B1, B2, B3, B4)
• 405 nm (solutions C1, C2, C3, C4)
Make a white with distilled water. Perform duplicated measurements.
Results:
Make a chart of the average absorbances found (axis y) according to
reference absorbances (axis x). You will obtain a chart like the following:
Example:
Wavelength: 340 nm
Temperature: 37 ºC
solution Abs. found Reference Abs.

water 0.000 0.000
A1 0.249 0.251
A2 0.489 0.502
A3 0.972 1.004
A4 1.406 1.506
readings vs references
1.5
1
Readings
0.5
0
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6
References
Ideal straight line Actual straight line
In order to calculate photometrical linearity, a study of linear regression

should be done for the straight line found:
Y=a+bX

Where b is the slope of the straight line and a the ordinate to the origin.
Slope b represents photometrical linearity. The ideal straight line would
be Y = X. Therefore, the ideal slope is 1.00, which indicates that the
instrument linearity responds in the scope of studied absorbances.
a y b can be calculated by the minimum squares method. There are

scientific calculators or computer programs –like Excel- which allow us to
make a linear regression and calculate such values, by entering the 4
data of the absorbances found and the 4 true absorbances.
Acceptability limits :
Ideal slope: 1.00

Optimal slope: between 0.98 and 1.02
Acceptable slope: between 0.97 and 1.03
In the previous example, if we perform the minimum squares study, we

obtain the equation:
Y = 0.947 X
Therefore, the slope of the straight line is 0.947 (error – 5.3 %), and it is not
within acceptable limits for the scope of studied absorbances (A1- A4).
In such a case, Linearity cab be studied up to the third point, that is, only
considering absorbances A1, A2 and A3. We obtain the equation:
Y = 0.970 X
The slope is within acceptable limits.
Conclusion: The instrument behaves in a linear way up to absorbances
approximately equal to 1.000 UA (A3). This instrument should not be used
to read absorbances higher than that value, as it loses response
sensitivity when encountering increases in concentration.
7 Diagrams
7.1 Identifying printed circuits

The Printed Circuit will be identified by a fantasy name (related with its
function) followed by letter r and the revision number, which will be made
up of 2 parts: the main revision indicator and the secondary revision
indicator, separated by a full stop.
The main revision indicator will be modified when a modification affecting

its function, size, etc is introduced to the printing, representing an important
change in its functions or geometry.

The secondary revision indicator will be modified when a minor change, like
a change of its components, footprints, component reordering, etc, is
introduced to the printing.
The resulting format will be NAME rX.Y.
The file related with the printed circuit will have the format NAME rXY.pcb
The electrical scheme corresponding to the printed circuit will have,

besides, an electrical scheme revision indicator which will be modified with
the changes in the scheme not involving changes in the printed circuit, for
ex. Changes in the value of its components. Revision format will be X.Y.Z,
and the file name will be NAME rXYZ.dsn.

7.2 Electrical and electronic scheme

A B C D E
Vertical Mov.
RS232C Diluter Controller
Controller
Diluter
4
Horizontal Mov. 4
Controller Robot
Preheater
Controller
Cuvettes Tray
Controller
Cuvettes
Tray
RS232C Main
Controller Heater
I2C BUS
Controller
3 3
Computer
Sample & Reagent
Tray Controller Refrigerated
Sample &
Refrigerated Reagent Tray
Tray Control
Washer
Controller Washer
2
+15 2
Photometer
Power +12 Controller
220Vac 50Hz Supplyes & +24
110Vac 60Hz Photometer
Distribution +5
Board +15/-15 Amplifiers

Controller
Pump Peristaltic
Controller
Pump
1
Clinical Chemistry Analizer 1
Title
Block Diagram
Document Number Rev

OEM BLOCK DIAGRAM r13.DSN 1.3
Drawn Approved
PM Eng. Pablo Müller
Size B Sheet 1 of 1
A B C D E
A B C D E
VCC VCC VCC
R153 R152 R154

MAIN CONTROLLER 5K6 5K6 5K6
VERTICAL CONTROLLER POWER INPUT
RST RST RST

4 11.0592MHz 4
COVERED COVERED SDA SDA SDA
!PELTIER-OFF !PELTIER-OFF SCL SCL SCL
HSh HSh HSh
LAMP-ALARM LAMP-ALARM 11.0592MHz 11.0592MHz LEVEL-ON
LAMP-ON LAMP-ON !11.0592MHz !11.0592MHz LEVEL
IMPACT
SYNC
Page 2
Page 3
Page 14
HORIZONTAL CONTROLLER
DILUTER COMMUNICATIONS CONTROLLER
IMPACT
RST SYNC
11.0592MHz
RST
SDA 11.0592MHz
SCL
HSh SDA
SCL
Page 13 HSh
3 3
Page 4
PREHEATER TEMPERATURE CONTROLLER
REACTION TRAY CONTROLLER
LEVEL-ON LEVEL-ON
LEVEL LEVEL IMPACT
IMPACT IMPACT SYNC
RST RST
11.0592MHz 11.0592MHz
SDA SDA
SCL SCL W-UP W-UP
HSh HSh
VACUUM Page 5
Page 12 SAMPLE-REAGENT TRAY CONTROLLER
IMPACT
HEATER TEMPERATURE CONTROLLER SYNC
RST RST
11.0592MHz 11.0592MHz
2 COVERED 2
SDA SDA
SCL SCL
HSh HSh
Page 11 Page 6
AMPLIFIER CONTROLLER FILTER WHEEL CONTROLLER
RST RST
11.0592MHz 11.0592MHz
SDA SDA
SCL SCL
HSh HSh
SYNC
Page 10 Page 7
PUMP CONTROLLER WASHER CONTROLLER
RST RST
LAMP-ON 11.0592MHz 11.0592MHz W-UP
1 1
LAMP-ALARM
SDA SDA
Title
!PELTIER-OFF SCL SCL
Controller - Hierarchical
HSh HSh
SYNC Document Number Rev
SYNC SYNC
OEM CONTROLLER r132.DSN 1.3.2
VACUUM VACUUM
Drawn Approved
Page 9
Page 8
Size B Sheet 1 of 15
A B C D E
A B C D E
VCC
11.0592MHz
VCC R93
22K
W7 U16A U16F
R94 R95
2 1 74HC14 74HC14
1
27 27
1 2 13 12
!11.0592MHz
4 2 4
W8
U20 U21 U23 U22
3
Y6 35 43 AD0 AD5 3 2 A5 A0 12 13 AD0 A0 10 11 AD0
EA AD0/P0.0 D0 Q0 A0 D0 A0 O0
11.0592MHz 42 AD1 AD2 4 5 A2 A1 11 14 AD1 A1 9 12 AD1
AD1/P0.1 D1 Q1 A1 D1 A1 O1
21 41 AD2 AD0 7 6 A0 A2 10 15 AD2 A2 8 13 AD2
X1(in) AD2/P0.2 D2 Q2 A2 D2 A2 O2
40 AD3 AD4 8 9 A4 A3 9 17 AD3 A3 7 15 AD3
C63 C64 AD3/P0.3 D3 Q3 A3 D3 A3 O3
39 AD4 AD7 13 12 A7 A4 8 18 AD4 A4 6 16 AD4
27p 27p AD4/P0.4 D4 Q4 A4 D4 A4 O4
38 AD5 AD1 14 15 A1 A5 7 19 AD5 A5 5 17 AD5
AD5/P0.5 D5 Q5 A5 D5 A5 O5
20 37 AD6 AD3 17 16 A3 A6 6 20 AD6 A6 4 18 AD6
X2 (out) AD6/P0.6 D6 Q6 A6 D6 A6 O6
36 AD7 AD6 18 19 A6 A7 5 21 AD7 A7 3 19 AD7
AD7/P0.7 D7 Q7 A7 D7 A7 O7
RST 10 A8 27 A8 25
RESET A8 A8
24 A8 1 A9 26 A9 24
A8/P2.0 OC A9 A9
25 A9 11 A10 23 A10 21
A9/P2.1 G A10 A10
SDA 14 26 A10 A11 25 A11 23
SDA P3.2/INT0 A10/P2.2 A11 A11
15 27 A11 A12 4 A12 2
LAMP-ALARM P3.3/INT1 A11/P2.3 74HC373 A12 A12
16 28 A12 A13 28 A13 26
!PELTIER-OFF P3.4/T0 A12/P2.4 A13 A13
17 29 A13 A14 29 A14 1
COVERED P3.5/T1 A13/P2.5 A14 A14
30 A14 FA15 3
A14/P2.6 A15
HSh 2 31 A15 FA16 2 A15 20
HSh P1.0/T2 A15/P2.7 A16 CS
SCL 3 FA17 30 !WR 27
SCL P1.1/T2X/SS A17 WR
BNK0 4 19 !RD !RD 22
P1.2/ECI RD/P3.7 RD
BNK1 5 18 !WR !FCS 22
3 P1.3/CEX0 WR/P3.6 CE 3
BNK2 6 32 !PSEN !FOE 24
P1.4/CEX1 PSEN OE IDT71256L35Y
!FPROG 7 33 ALE !FWE 31
P1.5/CEX2/MiSo ALE/P WE
8 13
P1.6/CEX3/SCk TxD/P3.1
9 11
P1.7/CEX4/MoSi RxD/P3.0 AT29C020-10JC
T89C51RC2-SLSIM
!ADDR_SEL
U25
1
VCC I1/CLK
A12 2 23
I2 O1
A13 3 22
I3 O2
A14 4 21
U18 C58 I4 O3 VCC
C59 A15 5 20
10/16 I5 O4 U24
10/16 2 1 BNK0 6 19
V+ C1+ I6 O5
BNK1 7 18 SDA 5 8
I7 O6 SDA VCC
6 3 BNK2 8 17
J22 V- C1- I8 O7
C56 4 !RD 9 16 SCL 6
SCM10S-W C2+ I9 O8 SCL
10/16 !PSEN 10 15
C57 I10 O9 LAMP-ON
5 !WR 11 14 7 4
6 1 C2- I11 O10 WC VSS
2 !FPROG 13 2
VCC 7 2 10/16 I12
RS-TxD 14 11 TxD AT24C64-10PI-2.7
8 3 T1Out T1In
9 4 ATF22V10C-10PC
RS-RxD 13 12 RxD
10 5 R1In R1Out
!RS-RTS 7 10 !RTS
T2Out T2In
!RS-CTS 8 9 !CTS
R2In R2Out
MAX232ACPE
VCC
VCC GND
3
U18 MAX232 16 15
1 1
VCC
U20 T89C51RC2 44 22
U19 2 !RESET 5 6 RST Title
!RST RST
DS1233-15 Controller - Hierarchical
U21 74HC373 20 10
GND U16C
RESET C61
74HC14 Document Number Rev
U22 IDT71256 28 14 PB TS-2 1n
1
U23 AT29C020 32 16
Drawn Approved
U25 22V10 24 12
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST
5V +Vp
4 U30 8 x MUR110 4
W9 U32
1 2 12
Ain0/P1.0
1 13
11.0592MHz RST/VPP Ain1/P1.1
14 V-A 5 9
P1.2 A +5
Y8 15 V-B 7 D36 D38 D39 D41 SANYO-DENKI Step-Syn
P1.3 B
5 16 V-C 10 4 Type 103-770-1640
X1(in) P1.4 C +Vm J5
17 V-D 12
P1.5 D
C76 C77 18 !V-INH1 2 V-OA
P1.6 OA 4
19 !V-INH2 U29A !VQ-INH1 6
P1.7 INH1
4 1 !VQ-INH2 11 3 V-OB
X2(out) INH2 OB 3
6 3
INT0/P3.2
7 LEVEL-ON !V-S1 2 V-S1 1 13 V-OC
INT1/P3.3 SEN1 OC 2
8 V-SETREF0 V-S2 15
T0/P3.4 SEN2
LEVEL 2 9 V-HOME 74HC08 14 V-OD
LEVEL P3.0/RxD T1/P3.5 OD 1
IMPACT 3 11 V-END 12 8
IMPACT P3.1/TxD P3.7 GND
11 D35 D37 D40 D42
2317SJ-04
!V-S2 13
AT89C4051-24PC L298N
U29D
LEVEL-ON
R151 R148 DISIPADOR
VCC
VCC
VCC VCC
R137
3 R140 10K 2 x .47 1W 3
VCC VCC
3
1K U31B
3
LM339N R142 U31C
5
+ 10K R144 LM339N
2 9
10K + R126 R149
4 14
- 22K 1K
8
-
R145
V-HOME
12
R138 15K
12
10K
R125
VCC VCC VCC

VCC
VCC VCC J6
R132
R141 10K R127 R122 1
3
1K U31A 22K 1K 2
3
LM339N R143 U31D 3
7
+ 10K R147 LM339N 4
1 11 V-END
10K + 5
2 6 13 2
- 6
10
- R124
R146 WF6S
12
R131 15K 12
10K
Sync
R129 R134
VCC V-SETREF0 = LO V-SETREF0 = HI
VCC VCC (!V-INH1 = HI) AND (!V-INH2 = HI) 0.663 V 0.165 V

R139
R133 100K
33K
!V-INH1 Q11 (!V-INH1 = LO) OR (!V-INH2 = LO) 0.935 V 0.233 V
BC327
R134 R129
33K 12K
!V-INH2 R136
27K
1 V-VRef 1
Title
R135
Controller - Vertical Hierarchical
560
R130
Document Number Rev
R128 1K8
22K
V-SETREF0 Q10
BC337 Drawn Approved
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U33 8 x MUR110
4 W10 U35 4
1 2 12
Ain0/P1.0
1 13
14 H-A 5 9
P1.2 A +5
Y9 15 H-B 7 D44 D46 D47 D49 SANYO-DENKI Step-Syn
P1.3 B
5 16 H-C 10 4 Type 103-770-1640
17 H-D 12
P1.5 D
C107 C106 18 !H-INH1 2 H-OA
P1.6 OA 4
19 !H-INH2 U36B !HQ-INH1 6
P1.7 INH1
4 4 !HQ-INH2 11 3 H-OB
X2(out) INH2 OB 3
6 6
INT0/P3.2
7 !H-S1 5 H-S1 1 13 H-OC
INT1/P3.3 SEN1 OC 2
8 H-SETREF0 H-S2 15
T0/P3.4 SEN2
2 9 H-HOME 74HC08 14 H-OD
P3.0/RxD T1/P3.5 OD 1
IMPACT 3 11 H-CHECK 9 8
8 D43 D45 D48 D50
2317SJ-04
!H-S2 10
AT89C4051-24PC L298N
U36C
R182 R179 DISIPADOR
VCC
VCC
VCC VCC
R168
R171 10K 2 x .47 1W
3
3 1K U34B 3
3
LM339N R173 U34C
5
+ 10K R176 LM339N VCC VCC
2 9
10K +
4 14
-
8
-
R175 R157 R180
12
R169 15K 22K 1K
12
10K
H-HOME
R156
VCC
VCC
VCC VCC VCC VCC
R163
R172 10K J4
3
1K U34A
3
LM339N R174 U34D R158 R150 1
7
+ 10K R178 LM339N 22K 1K 2
1 11
10K + 3
6 13
- 4
2 10 H-CHECK 2
- 5
R177 6
12
R162 15K R155
12
10K WF6S
Sync
R161 R167
VCC H-SETREF0 = LO H-SETREF0 = HI
VCC VCC (!H-INH1 = HI) AND (!H-INH2 = HI) 0.663 V 0.165 V

R170
R164 100K
33K
!H-INH1 Q13 (!H-INH1 = LO) OR (!H-INH2 = LO) 0.935 V 0.233 V
BC327
R165 R161
33K 12K
!H-INH2 R167
27K
1 H-VRef 1
Title
R166
Controller - Horizontal Controller
560 R160
R159 1K8
Document Number Rev
22K
H-SETREF0 Q12 OEM CONTROLLER r132.DSN 1.3.2
BC337
Drawn Approved
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U9 8 x MUR110
4 W4 U11 4
1 2 12
Ain0/P1.0
1 13
14 R-A 5 9
P1.2 A +5
Y4 15 R-B 7 D12 D14 D15 D17 SANYO-DENKI Step-Syn
P1.3 B
5 16 R-C 10 4 Type 103-807-6341
X1(in) P1.4 C +Vm J11
17 R-D 12
P1.5 D
C39 C38 18 !R-INH1 2 R-OA
P1.6 OA 4
19 !R-INH2 U12B !RQ-INH1 6
P1.7 INH1
4 4 !RQ-INH2 11 3 R-OB
X2(out) INH2 OB 3
6 6
INT0/P3.2
7 R-SETREF1 !R-S1 5 R-S1 1 13 R-OC
INT1/P3.3 SEN1 OC 2
8 R-SETREF0 R-S2 15
T0/P3.4 SEN2
W-UP 2 9 R-HOME 74HC08 14 R-OD
W-UP P3.0/RxD T1/P3.5 OD 1
IMPACT 3 11 R-CHECK 9 8
8 D11 D13 D16 D18
2317SJ-04
!R-S2 10
AT89C4051-24PC L298N
U12C
R60 R58 DISIPADOR
VCC
VCC
VCC VCC
R47 VCC VCC
R50 10K 2 x .22 1W
3
3 1K U10B 3
3
LM339N R52 U10C
5
+ 10K R55 LM339N R35 R59
2 9
10K + 22K 1K
4 14
-
8
-
R54 R-HOME
12
R48 15K
12
10K R33
VCC VCC
VCC J12
VCC
VCC VCC R36 R30 1
R41 22K 1K 2
R51 10K 3
3
1K U10A 4
R-CHECK
3
LM339N R53 U10D 5
7
+ 10K R57 LM339N 6
1 11
10K + R32
6 13
- WF6S
2 10 2
-
R56
12
R40 15K
12
10K
Sync
VCC
R37 R42 R44
R-SETREF0 = LO R-SETREF0 = LO R-SETREF0 = HI
VCC VCC R-SETREF1 = LO R-SETREF1 = HI R-SETREF1 = HI
R49
R42 100K
33K
!R-INH1 Q5 (!R-INH1 = HI) AND (!R-INH2 = HI) 0.470 V 0.313 V 0.117 V
BC327
R44 R39
(!R-INH1 = LO) OR (!R-INH2 = LO) 0.663 V 0.442 V 0.165 V
33K 12K
!R-INH2 R46
27K
R-VRef
R45 R43
470 2K2
1 1
R38
R37 1K2
Title
22K
R-SETREF0 Q4 Q3 Controller - Reaction TrayController
BC337 BC337
Document Number Rev
R34
Drawn Approved
22K
R-SETREF1 PM Eng. Pablo Müller
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U37 8 x MUR110
4 W11 U39 4
1 2 12
Ain0/P1.0
1 13
14 S-A 5 9
P1.2 A +5
Y10 15 S-B 7 D52 D54 D55 D57 SANYO-DENKI Step-Syn
P1.3 B
5 16 S-C 10 4 Type 103-807-6341
17 S-D 12
P1.5 D
C103 C104 18 !S-INH1 2 S-OA
P1.6 OA 4
19 !S-INH2 U36A !SQ-INH1 6
P1.7 INH1
4 1 !SQ-INH2 11 3 S-OB
X2(out) INH2 OB 3
6 3
INT0/P3.2
7 S-SETREF1 !S-S1 2 S-S1 1 13 S-OC
INT1/P3.3 SEN1 OC 2
8 S-SETREF0 S-S2 15
T0/P3.4 SEN2
2 9 S-HOME 74HC08 14 S-OD
P3.0/RxD T1/P3.5 OD 1
IMPACT 3 11 S-CHECK 12 8
11 D51 D53 D56 D58
2317SJ-04
!S-S2 13
AT89C4051-24PC L298N
U36D
R211 R209 DISIPADOR
VCC
VCC
VCC VCC
R198
R201 10K 2 x .22 1W
3
3 1K U38B VCC 3
3
LM339N R203 U38C VCC
5
+ 10K R205 LM339N
2 9
10K +
4 14
- R186
8
- 22K R210
R206 1K
12
R199 15K
S-HOME
12
10K
R184
VCC VCC VCC

VCC
VCC VCC J2
R192
R202 10K R187 R181 1
3
1K U38A 22K 1K 2
3
LM339N R204 U38D 3
7
+ 10K R208 LM339N 4
1 11 S-CHECK
10K + 5
6 13
- 6
2 10 2
- R183
R207 WF6S
12
R191 15K
12
10K
Sync
VCC
R191 R195 R197

VCC VCC S-SETREF0 = LO S-SETREF0 = LO S-SETREF0 = HI
R200
S-SETREF1 = LO S-SETREF1 = HI S-SETREF1 = HI
R193 100K
33K
!S-INH1 Q16
BC327 (!S-INH1 = HI) AND (!S-INH2 = HI) 0.470 V 0.313 V 0.117 V
R195 R189
33K 12K
!S-INH2 (!S-INH1 = LO) OR (!S-INH2 = LO) 0.663 V 0.442 V 0.165 V
R197
27K
S-VRef
R196 R194
470 2K2
1 1
R190
R188 1K2
Title
22K
S-SETREF0 Q15 Q14 Controller - Sample-Reagent Tray Controller
BC337 BC337
Document Number Rev
R185
22K
S-SETREF1 Drawn Approved
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U13 8 x MUR110
4 W5 U15 4
1 2 12
Ain0/P1.0
1 13
14 F-A 5 9
P1.2 A +5
Y5 15 F-B 7 D20 D22 D23 D25 SANYO-DENKI Step-Syn
P1.3 B
5 16 F-C 10 4 Type 103-770-1640
17 F-D 12
P1.5 D
C49 C50 18 !F-INH1 2 F-OA
P1.6 OA 4
19 !F-INH2 U12A !FQ-INH1 6
P1.7 INH1
4 1 !FQ-INH2 11 3 F-OB
X2(out) INH2 OB 3
6 3
INT0/P3.2
7 !F-S1 2 F-S1 1 13 F-OC
INT1/P3.3 SEN1 OC 2
8 F-SETREF0 F-S2 15
T0/P3.4 SEN2
2 9 F-HOME 74HC08 14 F-OD
P3.0/RxD T1/P3.5 OD 1
3 11 12 8
P3.1/TxD P3.7 GND
11 D19 D21 D24 D26
2317SJ-04
!F-S2 13
AT89C4051-24PC L298N
U12D
R86 R83 DISIPADOR
VCC
VCC
VCC VCC
R72
R75 10K 2 x .47 1W
3
3 1K U14B 3
3
LM339N R77 U14C
5
+ 10K R79 LM339N
2 9
10K +
4 14
-
8
- VCC VCC
R80
12
R73 15K
12
10K
R62 R84
22K 1K J10
1
F-HOME 2
VCC 3
VCC R61
VCC VCC WF3S
R67
R76 10K
3
1K U14A
3
LM339N R78 U14D
7
+ 10K R82 LM339N
1 11
10K +
6 13
-
2 10 2
-
R81
12
R66 15K
12
10K
Sync
VCC R64 R69
F-SETREF0 F-SETREF0
= LO = HI
VCC VCC
R74
(!F-INH1 = HI) AND (!F-INH2 = HI) 0.663 V 0.331 V
R68 100K
33K
!F-INH1 Q7
BC327 (!F-INH1 = LO) OR (!F-INH2 = LO) 0.935 V 0.467 V
R69 R64
33K 12K
!F-INH2 R71
27K
F-VRef
R70
1 1
1K
R65
Title
R63 1K5
Controller - Filter Wheel Controller
22K
F-SETREF0 Q6
BC337 Document Number Rev
Drawn Approved
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U26 8 x MUR110
W6 U28
1 2 12
Ain0/P1.0
1 13
4 11.0592MHz RST/VPP Ain1/P1.1 4
14 W-A 5 9
P1.2 A +5
Y7 15 W-B 7 D28 D30 D31 D33 SANYO-DENKI Step-Syn
P1.3 B
5 16 W-C 10 4 Type 103-770-1640
17 W-D 12
P1.5 D
C82 C81 18 !W-INH1 2 W-OA
P1.6 OA 4
19 !W-INH2 U29B !WQ-INH1 6
P1.7 INH1
4 4 !WQ-INH2 11 3 W-OB
X2(out) INH2 OB 3
6 6
INT0/P3.2
7 !W-S1 5 W-S1 1 13 W-OC
INT1/P3.3 SEN1 OC 2
8 W-SETREF0 W-S2 15
T0/P3.4 SEN2
2 9 W-UP 74HC08 14 W-OD
P3.0/RxD T1/P3.5 OD 1
3 11 W-DOWN 9 8
P3.1/TxD P3.7 GND
8 D27 D29 D32 D34
2317SJ-04
!W-S2 10
AT89C4051-24PC L298N
U29C
FLUSH-R FILL R123 R120 DISIPADOR
FLUSH
VCC
12V VCC 2 x .47 1W
VCC VCC
VCC R109
R112 10K
3
3 D63 1K U27B 3
3
FR107 LM339N R114 U27C VCC VCC
5
R215 + 10K R117 LM339N
2 9
R213 1K 10K +
4 14
100 -
Q20 8
- R98 R121
MJE800
R116 22K 1K
12
R110 15K
12
10K
W-UP W-UP
12V 12V R97
J27 VCC
VCC
1 D62 VCC VCC VCC
2 !FLUSH FR107 VCC VCC
3 R216 R104 J8
4 !FLUSH-R R214 1K R113 10K
3
5 100 1K U27A R99 R85 1
!FILL Q19
3
6 LM339N R115 U27D 22K 1K 2
MJE800 7
7 + 10K R119 LM339N 3
!VACUUM 1 11
8 10K + 4
6 13 W-DOWN
- 5
2 10 2
2317SJ-08 - 6
R118 R96
12
R103 15K WF6S
12
10K
12V VCC
Sync
D61
FR107 R217 VCC
C100 1K R100 R106
D64
100/35 W-SETREF0 = LO W-SETREF0 = HI
1N4148 VCC VCC
Q18
R111
MJE800
R105 100K
(!W-INH1 = HI) AND (!W-INH2 = HI) 0.663 V 0.331 V
33K
R218 Q21 !W-INH1 Q9
10K BC337 BC327
(!W-INH1 = LO) OR (!W-INH2 = LO) 0.935 V 0.467 V
R106 R102
33K 12K
!W-INH2 R108
27K
12V VCC W-VRef
1 1
R107
D60
R220 560
FR107 Title
1K R101
Controller - Washer Controller
R212 R100 1K8
100 22K
Q17 W-SETREF0 Q8 Document Number Rev
MJE800 VACUUM BC337 OEM CONTROLLER r132.DSN 1.3.2
Drawn Approved
A B C D E
A B C D E
HSh
HSh
SCL
SCL
SDA
SDA
RST 5V +Vp
U5 8 x MUR110
1 W3 2 12 U8
4 Ain0/P1.0 4
1 13
14 P-A 5 9
P1.2 A +5
Y3 15 P-B 7 D4 D6 D7 D9 SANYO-DENKI Step-Syn
P1.3 B
5 16 P-C 10 4 Type 103-770-1640
X1(in) P1.4 C +Vm J13
17 P-D 12
P1.5 D
C24 C23 18 !P-INH1 2 P-OA
P1.6 OA 4
19 !P-INH2 U6A !PQ-INH1 6
P1.7 INH1
4 1 !PQ-INH2 11 3 P-OB
X2(out) INH2 OB 3
6 3
INT0/P3.2
7 P-SCL !P-S1 2 P-S1 1 13 P-OC
INT1/P3.3 SEN1 OC 2
8 P-SDA-R P-S2 15
T0/P3.4 SEN2
P-SDA-C 2 9 P-HOME 74HC08 14 P-OD
P3.0/RxD T1/P3.5 OD 1
P-SDA-W 3 11 12 8
P3.1/TxD P3.7 GND
11 D3 D5 D8 D10
2317SJ-04
!P-S2 13
AT89C4051-24PC L298N
U6D
R31 R28 DISIPADOR
VCC
VCC
VCC VCC VCC VCC VCC VCC
R19
R15 10K 2 x .47 1W
3
1K U7B
3
3 R4 R3 R2 R1 VCC LM339N R17 U7C 3
5
5K6 5K6 5K6 5K6 + 10K R24 LM339N
2 9
J21 10K +
4 14
-
8
1 -
P-SDA-W 2 R25
P-SDA-R
12
3 R20 15K
P-SDA-C
12
4 10K
P-SCL 5
VCC VCC
LAMP-ALARM 6
!PELTIER-OFF 7
LAMP-ON 8
9 R8 R29
22K 1K J14
VCC
WF9S
VCC
1
VCC VCC P-HOME
R10 2
R16 10K 3
3
1K U7A R5
3
LM339N R18 U7D WF3S
7
+ 10K R27 LM339N
VCC 1 11
10K +
6 13
-
10
-
2 R26 2
12
R92 R11 15K
D2
1K
U16E
10K 12
R88
1N4148 74HC14
2K2
11 10
R64
C55
1n SYNC
VCC (!P-INH1 = HI) AND (!P-INH2 = HI) 0.663 V
R87
15K D1
1N4148
VCC VCC (!P-INH1 = LO) OR (!P-INH2 = LO) 0.935 V
VCC R14
R12 100K
33K
!P-INH1 Q2
BC327
8
R13 R23
2
- 33K 12K
1 !P-INH2 R22
3
VCC + U17A 27K
LM393N
P-VRef
4
1 1
R91
22K R21
Title
1K8
Controller - Pump Controller
R90
Document Number Rev
R89 22K
22K
Drawn Approved
A B C D E
A B C D E
4 4
HSh
HSh
SCL
SCL
SDA
SDA
RST
U2 J17
W1
1 2 12
Ain0/P1.0 1
1 13
11.0592MHz RST/VPP Ain1/P1.1 2
14 !DSYNC
P1.2 3
Y1 15 !DRDY0
P1.3 4
5 16 !DRDY1
X1(in) P1.4 5
17 !CS0
P1.5 6
C9 C8 18
P1.6 7
19
P1.7 8
4
X2(out) 9
6
INT0/P3.2 10
7 !CS1
INT1/P3.3 11
8 SCLK
T0/P3.4 12
A-RxD 2 9 MoSi
P3.0/RxD T1/P3.5 13
A-TxD 3 11 MiSo
3 P3.1/TxD P3.7 14 3
15
AT89C4051-24PC 16
17
VCC 18
19
+12Va 20
VCC 21
22
23
U1 C5 24
C7
10/16 25
10/16 2 1
V+ C1+ 26
6 3
J18 V- C1- -12Va SCM26S-W
C3 4
SCM10S-W C2+
10/16 C4
5
VCC 6 1 C2-
7 2 10/16
A-RS-TxD 14 11 A-TxD
8 3 T1Out T1In
9 4
A-RS-RxD 13 12 A-RxD
10 5 R1In R1Out
!A-RS-RTS 7 10 A-!RTS
T2Out T2In
2 2
!A-RS-CTS 8 9 A-!CTS
R2In R2Out
MAX232ACPE
1 1
Title
Controller - Amplifier Controller
Document Number Rev

Drawn Approved
A B C D E
A B C D E
4 4
HSh
HSh
SCL
SCL +Vp
SDA
SDA
J15
VCC 3
RST 2
U4 1 THERMOSTAT 50ºC
74HC08
W2 R9
1 2 12 4 Q1 DZ1
Ain0/P1.0 47 DT-4C-B01W-03
1 13 6 C-ONB IRFZ44 30V 1/2W
11.0592MHz RST/VPP Ain1/P1.1 5 x 27 Ohm 25 Watt
14 C-ON 5
P1.2 Vishay Dale RH25 1 5%
Y2 15 C-SDA
P1.3
5 16 C-SCL U6B
X1(in) P1.4
17
C20 C19 P1.5 VCC
18
P1.6
19
P1.7 U6C
4
X2(out)
6 10
INT0/P3.2
7 8
INT1/P3.3
8 9 !COVERED
T0/P3.4
2 9
3 P3.0/RxD T1/P3.5 3
3 11
P3.1/TxD P3.7 74HC08
AT89C4051-24PC
COVERED
VCC VCC
5V VCC
ADS1100 1
R6 R7 J16 5 +Vs
VCC 5K6 5K6 VDD
6 2
1 Vin+ Vout LM35DT
U3 4
2 SDA GND
C-SDA 3
C18 MAX232ACPE C15 C-SCL 3
10/16 4 SCL
10/16 2 1 1 3
V+ C1+ 5 Vin-
!COVERED 2
6 GND
6 3
J19 V- C1-
C13 4
SCM10S-W C2+ WF6S
10/16 C14
5
VCC 6 1 C2-
7 2 10/16
C-RS-TxD 14 11 C-TxD
8 3 T1Out T1In
2 9 4 2
C-RS-RxD 13 12 C-RxD
10 5 R1In R1Out
!C-RS-RTS 7 10 !C-RTS
T2Out T2In
!C-RS-CTS 8 9 !C-CTS
R2In R2Out
1 1
Title
Controller - Heater Temperature Controller
Document Number Rev

Drawn Approved
A B C D E
A B C D E
4 4
HSh
HSh
SCL
SCL
SDA
SDA 24V
J25
2
U42 1
THERMOSTAT 50ºC
12 U16B
Ain0/P1.0 R225 PW2S
1 13 74HC14 Q22
RST RST/VPP Ain1/P1.1 47 DZ2
14 !P-ON 3 4 P-ON IRFZ44
P1.2 30V 1/2W
15 P-SDA 10Ω 25W
P1.3
5 16 P-SCL
11.0592MHz X1(in) P1.4
17
P1.5 VACUUM
18
P1.6
19
P1.7
4
X2(out)
6
INT0/P3.2
7
INT1/P3.3
8
T0/P3.4
2 9 IO0
P3.0/RxD T1/P3.5
3 11 IO1
3 P3.1/TxD P3.7 3
AT89C4051-24PC
5V
VCC VCC
VCC
5V VCC VCC 5V ADS1100 1

R221 R219 J24 5 +Vs
5K6 5K6 VDD
6 2
1 Vin+ Vout LM35DT
4
2 SDA GND
3
3
4 SCL
1 3
LEVEL 5 Vin-
8 9 2
IMPACT 6 GND
7
U16D
LEVEL-ON 8
74HC14
WF8S
2 LEVEL DETECTOR 2
IMPACT DETECTOR
VCC VCC VCC
R226 R222
J26
1
2 IO0
3 IO1
4
WF4S R224 R223

1 1
Title
Controller - Heater Temperature Controller
Document Number Rev

Drawn Approved
A B C D E
A B C D E
4 4
HSh
HSh
SCL
SCL
SDA
SDA
RST
U40
W12
1 2 12
Ain0/P1.0
1 13
14
P1.2
Y11 15
P1.3
5 16
X1(in) P1.4
17
P1.5
C110 C109 18
P1.6
19
P1.7
4
3 X2(out) 3
6
INT0/P3.2
7
INT1/P3.3
8
T0/P3.4
2 9
P3.0/RxD T1/P3.5
3 11
P3.1/TxD P3.7
AT89C2051-24PC
VCC
U41
C113 MAX232ACPE C116
10/16 10/16
2 1
V+ C1+
6 3
V- C1-
4
C114 C2+
2 10/16 2
5 C115
J23 C2-
10/16
P-RS-TxD 14 11 P-TxD
1 T1Out T1In
2
P-RS-RxD 13 12 P-RxD
3 R1In R1Out
4
7 10 !P-RTS
T2Out T2In
WF4S
8 9 !P-CTS
R2In R2Out
1 1
Title
Controller - Diluter Communications Controller
Document Number Rev

Drawn Approved
A B C D E
A B C D E
5V
4 VR1 (*) VCC VCC 4
TPS75533KC
2 4
6 Vin Vout
5
1 5
J20 4 EN PG
JS-1152-06 3 C16 C33 GND C25 C102 C83 C51 C68
VSS 100/16 47/16
2 47/16 47/16 47/16 47/16 47/16
1
3
C88 C34 C28 C41 C53 C95 C78 C87 C101 C117 C10 C21 C26 C30 C48 C54 C62 C67 C73 C74 C112
1n .01 100n 100n .1 .1 .1 100n 100n .1 100n .1 100n 100n .1 .1 .1 100n .1 100n 100n
3 +12Va 3
C105 C111 C12 C22 C27 C37 C40 C47 C65 C75 C80 C84 C91 C97
.01 10n .01 10n .01 .01 10n 10n .01 10n 10n 10n .01 .01
C17 C2
100/16 .1
C6 C1
100/16 .1
C11 C29 C44 C52 C60 C66 C69 C70 C71 C72 C79 C94 C108 C118
1n 1n 1n 1n 1n 1n 1n 1n 10n 1n 1n 1n 1n 1n
-12Va
2 J29 1 2
2317SJ-02 2
12V
24V +Vp
1
J28
2
DT-4C-B01W-03 C31 C36 C43 C46 C86 C90 C93
3 C99 C98 D59
C85 C35 C32 C42 C45 C89 C92 C96 470/35 470/35 470/35 470/35 470/35 470/35 470/35
470/35 .1 1N5402
100n .1 100n 100n .1 .1 100n .1
(*) Para VCC = 5V no colocar TPS7533KC y puentear VR11-2 y VR11-4

Para VCC = 3.3V colocar TPS75533KC
1 1
Title
Controller - Power Input
Document Number Rev

Drawn Approved
A B C D E
A B C D E
4 4
3 3
2 2
1 1
Title
Controller - Layout
Document Number Rev

Drawn Approved
A B C D E
A B C D E
4 4
Level monitor Peltier current monitor
PELTIER-OFF
PELTIER-OFF PELTIER-OFF
LAMP-ON
LAMP-ON
LAMP-ALARM
LAMP-ALARM
Page 2 Page 3
3 3
12V and 5V Regulator Lamp Control
LAMP-ON
LAMP-ALARM
2 2
Page 4 Page 5
1 1
Title
Regulator - Hierarchical
Document Number Rev

OEM REGULATOR r112.DSN 1.1.2
Drawn Approved
Size B Sheet 1 of 6
A B C D E
A B C D E
VCC +12V VCC VCC
2
C16 C17 C15 C6 C7
100/16 .1 .1 D1 100/16 .1
U2A 1N5817
8
PS1 R17 LM358 U8
MPX10 1K 1% R13 R12
1
(+) 3 3 5
+ 1K 47 VDD
1 6
4 Vo+ 2 2 Vin+ 4 4
- SDA-C
SDA
2
Vo- 4 3 SCL
R16 SCL
4
1
(-) 91K 1% C5 D2 Vin-
1 2
1n 1N5817 GND
ADS1100
1
C8
R15
100/16 VCC
1K 1%
-12V
C32
1n
R14
270K 1%
VCC +12V VCC VCC J9
1
SDA-W
2
2
C19 C18 C11 C10 SDA-R
3
100/16 .1 D3 100/16 .1 SDA-C
4
3 U2B 1N5817 SCL 3
5
8
PS2 R23 LM358 U7 LAMP-ALARM 6
MPX10 1K 1% R20 R19 PELTIER-OFF 7
1
(+) 3 5 5
+ 1K 47 VDD LAMP-ON 8
7 6
Vo+ 2 6 Vin+ 4 9
- SDA-R
SDA
2
Vo- WF9S
4 3 SCL
R21 SCL
4
1
(-) 91K 1% C14 D4 Vin-
1 2
1n 1N5817 GND
C9 ADS1100
1
R22 .1
1K 1%
-12V
R18
270K 1%
2
VCC +12V VCC VCC 2
2
C21 C20 C13 C12
100/16 .1 D5 100/16 .1
U3B 1N5817
8
PS3 R28 LM358 U6
MPX10 1K 1% R25 R24
1
(+) 3 5 5
+ 1K 47 VDD
7 6
Vo+ 2 6 Vin+ 4
- SDA-W
SDA
2
Vo- 4 3 SCL
R29 SCL
4
1
(-) 91K 1% C23 D6 Vin-
1 2
1n 1N5817 GND
C22 ADS1100
1
R27 .1
1K 1%
-12V
1 1
R26
270K 1%
Title
Regulator - Level monitor
Document Number Rev

Drawn Approved
Size B Sheet 2 of 6
A B C D E
A B C D E
4 15Vp 15Vp 4
J6 J7
From 3 1
Power 2 R10 2 To Cooler
Supply 1 3 board
15Vp 15Vp
0.030
PELTIER-OFF
15Vp
C2 C3
47/25 .1
R4
1K
U1A
8
R7 LM358 U1B
8
10K LM358
3
+ R9
1 5
+ 10K
2 7 Q1
-
6 BC337
-
15Vp R1 C1 R8
4
1K .1 270K
4
R11
3 3
R5 2K2
R6
1K 1K
R3 R2
10K 270K
15Vp
P1
1
20K 25v
2 C4 2
.1
1 1
Title
Regulator - Peltier current monitor
Document Number Rev

Drawn Approved
Size B Sheet 3 of 6
A B C D E
A B C D E
24V 5V
U9
J4 LM323 J1
4 1 3 4
1 VIN VOUT 6
2 GND GND 5
3 C44 C43 C36 C37 4
470/35 .1 .1 470/16 3
2 4 2
1
24V
J5
3
2
1
24V 24V
J2 J3
2 2
3 1 1 3
+15V U11 +12V

J10 LM7812
1 3
3 VIN VOUT
2 GND
1 C34 C33 C39 C40
470/16 .1 .1 220/16
2
U10
LM7912
2 2 3 2
VIN VOUT
GND
C38 C35 C41 C42
470/16 .1 .1 220/16
-15V 1 -12V
1 1
Title
Regulator - 5V and +/- 12V Regulator
Document Number Rev

Drawn Approved
Size B Sheet 4 of 6
A B C D E
A B C D E
4 4
+15V
U4 +15V
LM350 J8 +15V
1 3
1
VIN VOUT 4 1
GND VO 2
C31 C30 R30 C25 C24 R31 3 P2 2
470/35 .1 100 .1 470/16 1K
2 25K 25v
+15V C27 C28
.1 47/25
C26 R32
3
.1 2K 25v
8
LAMP-ALARM
8
5V R35
3
3 + 10K R38 3
1 5
+ 10K
2 7 Q3
- U5A
6 BC337
LM358 - U5B
RX1 R36 LM358 R39
4
22K 33K C29 4 1K8
Q2 .1
R37 R33
47 IRF540 1K
LAMP-ON
R34
27K
2 2
1 1
Title
Regulator - Lamp Control
Document Number Rev

Drawn Approved
Size B Sheet 5 of 6
A B C D E
A B C D E
4 4
3 3
2 2
1 1
Title
Regulator - Layout
Document Number Rev

Drawn Approved
Size B Sheet 6 of 6
A B C D E
A B C D E
4 4
D0
D1
D2
D3
D4
D5
D6
D7
11
10
9
8
7
6
5
4
+5Va
C1 DB0 DB1 DB2 DB3 DB4 DB5 DB6 DB7
Note 1 2 I2 14
VDD
13
WR
12
R2 2R 2R 2R 2R 2R 2R 2R 2R CS
U2
Note 1
TLC7524 U3
3 +5Va VCC
R R R R R R R GND ADS1217IPFBT VCC VCC VCC
2R
Vref 1 31
AVDD DVDD
S1 13
+Va AVDD
VTB6061UVJ 37 D0
15
+12Va +5Va D0
38 D1
D1 R11 D5 R9
15 39 D2
IDAC1 D2 22K 1N4148 22K
16 40 D3
8
IDAC2 D3
17 41 D4
8
3 D2 RDAC D4 3
2 42 D5 1
- R R3 1N5817 R7 D5
1 16 Rfb I1 1 6 43 D6 W2
- 2K2 1% 1M5 1% D6
3 U1A 7 3 44 D7 +
+ Ain0 D7 C17
5 U1B 4 VCC
OP249FZ + Ain1 R10 10/25
2
5
OP249FZ D1 Ain2 22K
4
6 27
1N5817 Ain3 PDWN
4
7 24
Ain4 RESET J1
8 23
Ain5 BUFEN
-Va 9
Ain6 1
-12Va 10
Ain7 2
11 29 !DSYNC
+5Va AinCom DSYNC 3
C19 32 !DRDY 1 2 !DRDY0
DRDY 4
C5 .1 3 4 !DRDY1 5
15n 46 28 5 6 !CS0
VRefOut POL 6
33 !CS 7 8 !CS1
R5 CS 7
47 34 SCLK
100K 1% VRef+ SCLK 8
48 35 MoSi W3
VRef- DIn 9
36 MiSo Note 1
DOut 10
R4 11
W1 14 18
1M5 1% VRCap DGND 12
P1 1 2 Ioffset C9 19
DGND 13
100K 22v 1n 20
DGND 14
2 21
AGND DGND 15
2 12 22 2
AGND DGND 16
45 30
Xout
AGND DGND 17
Xin
VCC 18
VCC VCC
R1 19
+Va
25
26
100K 1% 20
Y1 21
C18 C16 22
1n .1 23
-5Va
24
2.4576MHz 25
26
C14 C15
18p 18p -Va SCM26S-W
Note 1: +5Va +12Va

R6
1K
Sample Channel: D4
C1 = 15n 1N5817
R1 = 1M 1% +
C7 C8 VR1 C6 C4 C12
W3,1 - W3,2
.1 1n LM336-5.0V .1 1n 47/16
W3,5 - W3,6
Reference Channel:
1 C1 = 2n2 + D3 1
C11 C10 VR2 C2 C3 C13
R1 = 6M8 1% 1N5817
.1 1n LM336-5.0V .1 1n 47/16
W3,3 - W3,4 Title
W3,7 - W3,8 Amplifier
R8
Document Number Rev
1K
-5Va -12Va OEM AMPLIFIER r121.DSN 1.2.1
Drawn Approved
Size B Sheet 1 of 2
A B C D E
A B C D E
4 4
3 3
2 2
1 1
Title
Amplifier - Layout
Document Number Rev

OEM AMPLIFIER r121.DSN 1.2.1
Drawn Approved
Size B Sheet 2 of 2
A B C D E
A B C D E
4 VCC VCC 5V 5V 4
C2 C4 C1 C3
.1 47/16 .1 47/16
VCC 5V
VCC 5V VCC
U1 D1 1
J1 1N5817 R1
5 +Vs S1
VDD 1K
6 2 LM35DT
1 Vin+ Vout
4
2 SDA GND
SDA R2
3
SCL 3 C5 220
4 SCL D2
1 .1 3
5 Vin- 1N5817
2
6 GND
wf6s ADS1100IDBVT
VCC
3 3
R3
1M
Q1
L14Q1
2 2
1 1
Title
Heater
Document Number Rev

OEM HEATER r121.DSN 1.2.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
VCC 5V 5V
C3 C1 C2
4 .1 .1 47/16 4
VCC
5V
VCC VCC THERMOSTAT XXºC
5V 1
U2 D3 J2
J3 1N5817 R7
5 +Vs
VDD 47 1
6 2
1 Vin+ 2 Vout LM35CAH
4
2 SDA 3 GND
SDA 15V 25W
3
SCL 3 3
4 SCL D4 WF3S
LEVEL 1
5 Vin- 1N5817
!IMPACT 2
6 GND
7
8 LEVEL-ON ADS1100IDBVT
WF8S
5V
VCC 5V
3 3
R8 U1B R1
47 R3 10M
8
LM393N
22K
6
-
7
5
+ 5V
4
R2
100K
5V R4 5V
VCC
56K
U1A
8
R6 LM393N D2
1N4148 J1
22K 3
+
1
1
2
- 2
2 2
R5 D1
1N4148 WF2S
4
22K
VCC
R9
22K
S1 S2 S3
S4
PB TS-4 PB TS-4 PB TS-4 .1
1 1
Title
Preheater
Document Number Rev

OEM PREHEATER r121.DSN 1.2.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
4 4
J1
1
2
3 3 3
1
3
WF3R
OP1
TCST1103
2
4
2 2
1 1
Title
Sensor P
Document Number Rev

OEM SENSORP r121.DSN 1.2.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
4 4
J2
1
2
3
WF3R
J1
1
3 2 3
3
1
3
WF3R
OP1
TCST1103
2
4
2 2
1 1
Title
Sensor R
Document Number Rev

OEM SENSORR r111.DSN 1.1.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
4 4
J1
1
2
3
3 3
1
3
WF3R
OP1
TCST1103
2
4
2 2
1 1
Title
Sensor F
Document Number Rev

OEM SENSORF r111.DSN 1.1.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
VCC VCC VCC
VCC
R24
8
5K6
8
+15 3 5
VR1 VCC J5 2 +
3 7
J10 LM7812 1 +
1 6
C13 - U4B
1 3 2
4 3 VIN VOUT .1 - U4A LM358 4
2 GND LM358
4
1
4
C27 C28 C10 C2 C22 C4 C9 C26 C6 C17 C19 C23
.1 100/25 2 100/16 100/16 100/16 .1 .1 .1 10n 10n 10n 10n D3
C16
.1
GND +15
R25 J3
5K6 1N4148
R26 C14 R23 3
1K .1 1K5 2
R27 1
68K
VCC VCC VCC VCC VCC J2
VCC VCC
3
2
R11 R12 R8 R14 1
R15
8
8
15K 2K2 15K 10K

VCC 1K
C7 6 11 9 3 Q2
J4 - CON OUT +
10n 7 8 VCC 1 IRFZ44N
TRG R18
Q3 5 12 C11 2
1 + U1B THRES D2 330K R19 - U3A R20
BC337 VCC 13 10 10/16
2 LM393 C8 DIS RST 1N4148 680K LM393 10K
Q4
3 3 .1 3
4
4
U2B BC337
R10 R9
LM556
1K2 47 R4 R16
15K 2K2
VCC VCC VCC
VCC VCC
C1 R6
.1 4K7 R7 R13 D1
8
15K 10K 1N4148 R17
R1 R5 330K
3 3 5
15K 2K2 + CON OUT
VCC 1 6 VCC
C3 2 2 TRG
J1 - U1A THRES
10n 1 4 VCC
LM393 C5 DIS RST
Q1
1 .1 +15
4
BC337 U2A
2 LM556 J8
3 R21
R2 R3 15K 3
1K2 47 2
1
C12 R22 J7
.1 10K VCC
2 VCC 2
VCC VCC VCC VCC VCC 3
2
U3B 1
R32
8
LM393
R41 R40 R37 R36 D5 1K
6 Q7
8
15K 2K2 15K 10K 1N4148 R29 -
VCC 7 IRFZ44N
330K
C20 6 11 9 5
J6 - CON OUT +
10n 7 8 VCC
TRG R31
Q6 5 12 C15
1 + U6B THRES R28 10K
4
BC337 VCC 13 10 10/16 Q5

2 LM393 C21 DIS RST 680K BC337
3 .1
4
U5B R33
R39 R38
LM556 2K2
1K2 47 R43
15K
VCC VCC VCC
VCC VCC
C24 R42
.1 4K7 R34 R35 D4
8
15K 10K 1N4148 R30
R46 R47 330K
3 3 5
15K 2K2 + CON OUT
VCC 1 6 VCC
1 C25 2 2 TRG 1
J9 - U6A THRES
10n 1 4
LM393 C18 DIS RST
Q8 Title
1 .1
4
BC337 U5A Cooler
2 LM556
3 Document Number Rev
R45 R44
OEM COOLER r121.DSN 1.2.1
1K2 47
Drawn Approved
Size B Sheet 1 of 2
A B C D E
A B C D E
4 4
3 3
2 2
1 1
Title
Cooler - Layout
Document Number Rev

OEM COOLER r121.DSN 1.2.1
Drawn Approved
Size B Sheet 2 of 2
A B C D E
A B C D E
4 4
NEG
J1
3 1 3
2
3
4
POS
U1
LM7812 J2
1 3
VIN VOUT 1
GND 2
2
C1
10/25
2 2
1 1
Title
Brush
Document Number Rev

OEM BRUSH r111.DSN 1.1.1
Drawn Approved
Size B Sheet 1 of 1
A B C D E
A B C D E
Amber Switch
4 4
MEAN WELL
CORCOM SP320-24
Line Filter 24V 12.5A
6EGS1-2
MEAN WELL
3
S35-12 3
12V 3A
MEAN WELL
ID-50B
+/- 13.8V
2 2
1.8A
Green Switch
MEAN WELL
SP150-15
15V 10A
1 1
Title
WIRING - AC Input
Document Number Rev

OEM WIRING r11.DSN 1.2
Drawn Approved
Size B Sheet 1 of 3
A B C D E
A B C D E
4 x SP-2003-2PK Motor 103.770.1640

SP3000
Cavro Diluter XL-3000 Motor 103.770.1640
SensorR r1.1 SensorR r1.1
J1 J1
Peristaltic Pump
4 4
J23 J7 J8 Washer and Pumps

J27
J13
J20
SP200-24 Sample Motor 103.807.6341 Motor 103.770.1640
J1
Reagent Filter whell Amplifier r1.2
J1 Tray
J5 J4 J15 CONTROLLER r1.3 SensorP r1.2 SensorF r1.1 J1
J1 J1 Amplifier r1.2
J28
J1
REGULATOR r1.1 J2 Lamp
J9 998319-35
S35-12 J10 Photometer
Motor 103.770.1640 Motor 103.770.1640
J17
3 Horizontal Vertical 3
SensorR r1.1 SensorR r1.1

J1 J1
J9 J21
J3
J10 Robot
J4
ID50-B J2 J5
Cuvettes
J6 J6 Tray Motor 103.770.1640
J7 J8 J3
SensorP r1.2
J1
J11
2 2
J12
J24 J3 J1
Preheater r1.2 Needle
J25 J2
J22 J16
Preheater LM35DT
SP150-15 Holster
RS232 To PC
To BRUSH r1.1 J1 DB9 P
(WIRING sheet 3 of 3)
Cuvettes
1 2 x 24Vcc FAN 1
J1 Tray
Heater Title
Container WIRING - General
Heater r1.2
Document Number Rev
Drawn Approved
Size B Sheet 2 of 3
A B C D E
A B C D E
FAN PELTIER
4 4
From Regulator r1.1 J7 BRUSHr1.1 Brush

(WIRING sheet 2 of 3)
J1
J1
J2
J2
J3
FAN PELTIER
COOLER r1.2
J4
3 3
J5
J7 FAN PELTIER
J8
J9
J10
J6
BASE FAN
2 2
FAN PELTIER
LM35DT
1 1
Title
WIRING - Reagent Cooler
Document Number Rev

Drawn Approved
Size B Sheet 3 of 3
A B C D E
7.3 Mechanical scheme

Header Module
Robot Module
Cuvettes Tray Module
Sample-Reagents Tray Module
Washer Module
Photometer Module
7.4 Hydraulic Scheme

APENDIX A: Spare parts list
FS0001 Single mouth bottle
FD0001 Double mouth bottle
TM0001 13 mm Tube
TF0001 Caps for single and double mouth bottles
LP0001 Lamp
TB0001 Kit x 2 peristaltic pump’s Tubings
ST0001 Strips with 10 cuvettes each
FU0001 4 A Fuse
JG0001 500µl Syringe
DI0001 Diluter
TB0002 Pump - Diluter Tubing
TB0003 Diluter - Head Tubing
TB0004 Vessel - Peristaltic Pump Tubing
TB0005 Vessel - Washer Pump Tubing
TB0006 Washer Head Tubing
AJ0001 Probe
CP0001 Peristaltic Pump
CH0001 Union NF ¼-28 x 5/8”
CH0002 Hose - Hose Connector

CH0003 Hose - Union Connector
PS0001 15VDC Power Supply
PS0002 24VDC Power Supply
PS0003 ±15 VDC Power Supply
SM0001 770 Stepper Motor
SM0002 807 Stepper Motor
PG0001 Rubber foot
TP0001 MXL x 16 Pulley
TC0001 MXL x 130 Belt
TP0002 XL x 16 Pulley
TC0002 XL x 140 Belt
TC0003 XL x 165 Belt
PP0001 Peltier Module
CO0001 Cooler
IF0340 Interferential Filter 340 nm
IF0450 Interferential Filter450 nm

LE0001 Plain-Convex lens BK7
BD0001 Diaphragm Pump
CV0001 Check Valve
PAM121 Ready-built Board Amplifier r121
PBR111 Ready-built Board Brush r111
PCO132 Ready-built Board Controller r132
PCL111 Ready-built Board Cooler r111
PHE121 Ready-built Board Heater r121
PPR121 Ready-built Borrad Preheater r121
PRE112 Ready-built Board Regulator r112
PSF111 Ready-built Board SensorF r111
PSP122 Ready-built Board SensorP r122
PSR111 Ready-built Board SensorR r111

Sapphire 350 Service Manual

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Sapphire 350 Service Manual

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Service Manual

Moving the instrument ..................................................................................... 3

Placing and preparing the instrument......................................................... 5

Sapphire 350 Service Manual - SSM0405 I

4.1 Daily Maintenance..............................................................................44

4.2 Weekly maintenance .........................................................................44

4.3 Six-monthly maintenance ..................................................................44

Sapphire 350 Service Manual - SSM0405 II

Sapphire 350 Service Manual - SSM0405 III

Sapphire 350 Service Manual - SSM0405 1

Warning about the instrument and laboratory practice

1) The use of calibrators is recommended, no matter which method is

Sapphire 350 Service Manual - SSM0405 2

Instrument installation must be performed by especially trained personnel.

Electrical requirements: a standard 220 Volts 50 Hz or 110 Volts 60 Hz socket

Kind of water: bidistilled, approximate consumption 1.6 ml/det.

Relative humidity: 40 – 85% (no condensation)

Moving the instrument

Avoid knocking and/or vibration of any kind while moving.

Sapphire 350 Service Manual - SSM0405 3

Sapphire 350 Service Manual - SSM0405 4

5) Remove cardboard protecting the hoses.

Placing and preparing the instrument

The instrument must be placed onto a table in a clean environment, free

Sapphire 350 Service Manual - SSM0405 5

Interconnect the instrument to the computer and its peripherals before

Sapphire 350 Service Manual - SSM0405 6

Sample and reagent tray:

Sapphire 350 Service Manual - SSM0405 7

Sapphire 350 Service Manual - SSM0405 8

2.1 Connection to electrical supply:

2.2 Connection to a computer

Sapphire 350 Service Manual - SSM0405 9

Sapphire 350 Service Manual - SSM0405 10

3.1 Modules functional description

3.1.1 Description of Sapphire autoanalyzer

Sapphire autoanalyzer has been conceived with the latest technology in

Sapphire autoanalyzer is divided in the following modules:

Sample and reagent tray

This tray allows 30 reagents, all of them cooled. The minimum

Sapphire 350 Service Manual - SSM0405 11

Cuvettes tray or reaction tray

Sapphire 350 Service Manual - SSM0405 12

Each cuvette is in time washed by about 1600 µl of washing solution.

This system is made up of:

• Light source: A 12 volts 20 Watts halogen lamp is used, with a high

Sapphire 350 Service Manual - SSM0405 13

3.1.2 Description of the working cycle

The operating cycle can be divided in three parts:

Reaction preparation cycle

This cycle stages are:

Sapphire 350 Service Manual - SSM0405 14

Cuvette washing cycle

The preparation cycle is simultaneous to the reading cycle which

3.2 Operation from a terminal program

Firmware calibrations keep all the variables on each module. These

Hardware adjustment consists in adjusting the Reference and Sample

Note: This adjustment is only indicated when changing the preamplifiers.

We can also verify module operation, the sate of detectors according to

Sapphire 350 Service Manual - SSM0405 15

Note: Once the Hyperterminal has been activated, we advise analyzing

In this chapter It will be explained how to configurate the Hyperterminal

From the program Windows click on:

Type Sapphire in the Name box and accept.