Trityl-ChemMatrix ®

CM N
H

OH

Trityl-ChemMatrix is mainly used to obtain a protected peptide Hints and Tips

as is has very low TFA cleavage conditions (under 1%). This Due to their superior chemical accessibility and performance,
resin therefore has the same cleavage conditions as the Cl- ChemMatrix resins swell more than polystyrene resins. Make
Trityl-polystyrene. On request, Trityl-ChemMatrix resins can use reaction vessel that will accommodate a slight increase
be offered preloaded will all 20 natural amino acids. Other in volume to avoid any overflowing.
amino acids can be preloaded on special request. Please take
note that the Cl-Trityl-ChemMatrix resin is not offered for
Ordering Information
stability reasons. It is most effective to start with the Trityl-
OH-ChemMatrix resinchlorinate the resin and then add the ChemMatrix Product Available
amino acid of desire. Resins Number Quantities
Trityl OH-ChemMatrix 7-420-1310-5 5g

Category: Peptide acid resin Trityl OH-ChemMatrix 7-420-1310-10 10 g

Cleavage conditions: TFA < 1% Trityl OH-ChemMatrix 7-420-1310-25 25 g

Loading: 0.40-0.65 mmol
(Trityl-OH ChemMatrix)

Bead size: 100-200 mesh dry

General Procedures for solid phase peptide synthesis
(SPPS) using ChemMatrix Resins
ChemMatrix resins are designed to be used under identical
Swelling conditions to polystyrene. The only difference is that due to the
enhanced solvent and reagent accessibility of the ChemMatrix
resin, which gives rise to superior reaction and chemistry
performance, the resin may swell more in most solvents
(including TFA). All standard solvents used in SPPS such as DMF,
NMP, DCM and MeOH are compatible with ChemMatrix resins
(see Figure 1). The ChemMatrix resins are fully compatable with
the Syro family of Biotage peptide instruments.

Recommended Protocol - General Advice

Figure 1: Comparison of ChemMatrix and polystyrene swelling profiles in a
variety of SPPS solvents. The increased swelling in the case of ChemMatrix
confers much greater solvent and reagent accessibility and thereby reduced
the occurrence of by products or impurities such as incomplete couplings and
deletion sequences.
Please note that the following procedure can be further
optimized with alternative coupling agents, depending on the
coupling system and requirements, for examples: HBTU/HOBt
or HCTU/6-Cl HOBt for PyBOP/HOBt or HATU/HOAt. N,N-
diisopropylethylamine can also be replaced by weaker bases
such as N-methylmorpholine if racemization is a specific risk
to your peptide.
 The cleavage step relies on the use of TFA. Depending on the peptide sequence, all kind of cleavage
cocktails could be used. If Arg(Pbf) is present in the sequence, the cleavage should be left 3 hours
instead of one.

Coupling step (Representative Example)

1. Swell the resin with DMF (10 mL/g of resin);
2. Dissolve the Fmoc-amino acid (3 eq.) and HCTU (3 eq.)
in a minimum of DMF followed by the addition of NMM (N-Methylmorpholine) (4-5 eq.);
3. Add this solution to the swelled resin;
4. Stir 30 minutes at room temperature;
5. Filter and wash with DMF (3-4 times);
6. Add a solution of 20% piperidine in DMF (10-12 mL/g) to the resin and stir 30 minutes;
7. Filter and wash with DMF (3-4 times);
8. Begin the coupling of a new amino acid.

Cleavage step (Representative Example)

1. Following coupling reaction, wash the resin with methanol and then with Et2O;
2. Dry the resin for several hours under vacuum at room temperature;
3. Add 20 mL/g of a solution of TFA / H2O or DCM / Et3 SiH (1-10/96-87/2.5) and stir one hour
(3 hours if Arg(Pbf) are present); Guideline: TFA + (H20 or DCM) = 97.5%
4. Filter and rinse the resin twice with TFA;
5. Evaporate all the solvents to a minimum;
6. Under stirring, add cold Et2O (10-15 mL/mL of residual volume of solvent with the peptide);
7. Centrifugate the suspension and remove the supernatant ether;
8. Repeat step 6 TWICE more;
9. Dry the peptide under vacuum overnight (freeze drying could be also performed with a suitable
solvent).

Typical Trityl alcohol-ChemMatrix:

Chlorination and Coupling Procedures
• In a vessel for SPPS, 7.5 g of resin is added then swelled with 100 mL of dry DCM;
• Add 2 mL of SOCl2 (to get a final 2% SOCl2 in DCM);
• The vessel is shaked overnight;
• Drain the resin and rinse it with DCM (5x75 mL) then with NMM 2%/DCM (3x75 mL);
• Add a solution of the desired Fmoc-amino acid-OH (3eq.) with NMM (4 eq.) in DCM; www.biotage.com
• The vessel is shaked overnight; NORTH AMERICA
• Add 2.5 mL of a solution of 25% NMM / MeOH and shake for an hour; Main Office: +1 704 654 4900
Toll Free: +1 800 446 4752
• Drain the resin and rinse it with DCM, DMF, MeOH and ether (3x75 mL each). Fax: +1 704 654 4917
• Dry the resin overnight at room temperature (never heat it) Order Tel: +1 704 654 4900
press (4) at the auto attendant
Order Fax: +1 434 296 8217
ordermailbox@biotage.com

EUROPE
Main Office: +46 18 56 5900
Fax: +46 18 59 1922
Order Tel: +46 18 56 57 10
Order Fax: +46 18 56 57 05
order@biotage.com

JAPAN
Tel: +81 422 28 1233
Fax: +81 422 28 1236
jp_order@biotage.com

To locate a distributor please

visit our web site at
www.biotage.com.
© 2010 Biotage. All rights reserved. All brand and product names are trademarks or registered trademarks of their respective companies. The
information contained in this document is subject to change without notice.

CM-TRIT-0810