Microscope

Experiment #1
I. Overview
▪Having been constructed in the 16th
Century, microscopes have revolutionized
science with their ability to magnify small
objects such as microbial cells, producing
images with definitive structures that are
identifiable and characterizable.
I. Overview
▪Microscopes are instruments that are used in
science laboratories, to visualize very minute
objects such as cells, microorganisms, giving a
contrasting image, that is magnified.
▪Microscopes are made up of lenses for
magnification, each with their own
magnification powers.
II. Objectives
▪To familiarize yourself with the basic parts of the
microscope and general functions of the following
parts: eyepiece, objectives, stage, mechanical stage,
revolving nosepiece, diaphragm and course & fine
adjustment knobs
▪To be able to define magnification and determine
the magnification produced by each objective.
Microscope
▪One of the most important tools used to
study living things.

▪“Micro” - very small

▪“Scope” - to look at
Parts of
Microscope
 Eyepiece
Body Tube

Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Adjustment Knob
Diaphragm
Fine Adjustment Knob
Light
Base
Eyepiece
Eyepiece
▪Where you look
through to see the
image of your
specimen.
Body Tube
Body Tube
▪Connects the
eyepiece to the
objective lenses.
Arm

▪Supports the tube

Arm and connects it to the
base.
Revolving Nosepiece

▪Part that holds two or

Revolving Nosepiece
more objective lenses
and can be rotated to
easily change power.
Objective Lens

▪Usually you will find 3

or 4 objectiveObjective
lenses on Lens
a microscope.
Objective Lens
▪The shortest lens is the lowest power, the
longest one is the lens with the greatest power.
▪Scanner – 4x
▪Low Power Objective – 10x
▪High Power Objective – 40x
▪Oil Immersion Objective – 100x
Stage
▪Flat platform
where you place
Stage your slides.
Stage Clip

▪Hold the slides in

place.
Stage Clips
Diaphragm
▪Used to vary the
intensity and size of the
light that is projected
upward into the slide.
Diaphragm
Light
▪Makes the specimen
easier to see

Light
Base

▪Bottom part of the

microscope

Base
Coarse Adjustment Knob
▪Moves stage up and
down.

Coarse Adjustment Knob

Fine Adjustment Knob
▪Small, round knob
on the side of the
microscope used to
fine-tune the focus
Fine Adjustment Knob
of your specimen.
 12.
1.

2.
11.
3.
10.
4.
9.
5.
8.
6.
7.
III. Procedures
1. Identify the various parts of the
microscope.
2. Draw a diagram of the microscope and
label its various parts.
IV. Results
V. Questions
1. What are the different magnifications on a
microscope?
2. What type of microscope is most used in
science classes?
3. What is the difference between a stereo
microscope and a compound microscope?
V. Questions
4. Should I get a monocular microscope, a
binocular microscope, or a trinocular
microscope?
5. Why do I need to start with the lowest
magnification setting when using a
microscope?
VI. Conclusions