FORM 4 FINAL EXAMINATION

BIOLOGY

Time allowed: 2 hours

Name:

Class: F.4

Class No:

Teacher:

Instructions:
1. Answer ALL questions in Section A on Multiple Choice Answer Sheet.
2. Answer ALL questions in Section B in the spaces provided in Question-Answer Book.
3. The diagrams in this paper are NOT necessarily drawn to scale.
4. At the end of the examination, hand in the Multiple Choice Question Paper, the Multiple Choice
Answer Sheet and the Question-Answer Book separately.

Section A: Multiple Choice Questions (40 marks)

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1. Peter observed a prepared slide under a light microscope. He found a small unknown object at
low-power magnification. The object did not move when he moved the slide. He turned the
nosepiece to select a high-power objective. The object was still seen. This unknown object was
most probably on
A the objective.
B the eyepiece.
C the slide.
D the mirror.

Directions: The following two questions refer to the table below, which shows the relative abundance of
different organelles in four types of cells.
Cell Relative abundance
type Endoplasmic reticulum Mitochondrion Chloroplast
R + +++ +
S + + 
T +++ + 
U + +++ 
Key: Number of ‘+’ indicates the relative abundance of the organelle;
‘’ indicates the absence of the organelle

2. Which cell type is most likely the root hair cell?

A R
B S
C T
D U

3. Which cell type is most likely found in the wall of a capillary?

A R
B S
C T
D U

4. Which of the following are the functions of nucleic acids?

(1) carry genetic information
(2) as a component of cell membrane
(3) determine the features of organisms
A (1) and (2) only
B (1) and (3) only
C (2) and (3) only
D (1), (2) and (3)

5. Which of the following features helps identify a cell as a eukaryotic cell?

A absence of chloroplasts
B absence of vacuoles
C presence of cell wall
D presence of mitochondria
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6. A sample of organic compounds was tested and found to contain carbon, hydrogen, oxygen,
nitrogen and sulphur atoms only. The compounds are most likely
A phospholipids.
B cellulose.
C proteins.
D nucleic acids.

7. Invertase is an enzyme that catalyses the following reaction.

sucrose glucose + fructose

Invertase solution was added to sucrose solution in a test tube. The tube was then incubated at a
constant temperature. The graph below shows the rate of breakdown of sucrose in the reaction
mixture over time.

rate of
breakdown of
sucrose
(arbitrary unit)

0 40 80 120 160
time (min)

Which of the following statements about the enzymatic reaction is/are correct?
(1) In the first 40 minutes, the reaction rate was the highest.
(2) The amount of glucose in the reaction mixture remained constant in the first 40 minutes.
(3) Invertase was used up after 120 minutes.

A (1) only
B (2) only
C (1) and (2) only
D (1) and (3) only

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8. In an experiment, amylase was extracted from human body. Same volume of amylase solution was
added to three test tubes, X, Y and Z. The tubes were then treated as follows:
Tube X: kept at 25 °C
Tube Y: cooled to 0 °C and then heated up to 25 °C
Tube Z: heated up to 100 °C and then cooled to 25 °C

Which of the following correctly compares the activities of amylase in the three tubes after the
treatments?

A X>Y=Z
B X=Y>Z
C Z>Y=X
D Z=Y>X

9. Which of the following statements about enzymes is not correct?

A Enzymes are proteins that can catalyse biochemical reactions.
B Enzymes are formed at ribosomes.
C Enzymes are specific in action.
D Enzymes are killed at high temperatures.

10. The diagram below shows an enzyme molecule.

active site

Which of the following molecules is most probably the substrate for this enzyme?

A B

C D

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11. The graph below shows the change in the amount of products in the reaction mixture of an
enzymatic reaction.

accumulative
amount of
products

time

Which of the following graphs shows the effect of adding an inhibitor into the reaction mixture?

A B
with inhibitor accumulative amount
without inhibitor
accumulative amount

without inhibitor
of products
of products

with inhibitor

time time

C D
accumulative amount

without inhibitor
accumulative amount

with inhibitor

without inhibitor with inhibitor

of products
of products

time time

12. An investigation was carried out to study the effect of pH on the activity of an enzyme. Four test
tubes were set up. Each tube contained a mixture of enzyme and starch solutions. Buffer solutions
of different pH were added to each tube and the tubes were kept at 30 °C. The time taken for all the
starch in different tubes to break down is shown in the table below:
pH Time taken for all the starch to break down (min)
3 10.0
7 3.5
10 8.5
12 15.0

Which of the following is most probably the optimum pH for this enzyme?
A pH 3
B pH 7
C pH 10
D pH 12

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Directions: The following two questions refer to the diagram below, which shows an enzymatic
reaction.

enzyme
+ H2O +
HO O OH HO OH HO OH

P Q R

13. The reaction is an example of

A condensation.
B oxidation.
C hydrolysis.
D hydrogenation.

14. If Q is glucose, which of the following is a possible combination of P and R?

P R
A maltose glucose
B maltose fructose
C sucrose glucose
D lactose fructose

15. Which of the following correctly arranges the foods in a meal, from eating most to eating least, in a
balanced diet?
(1) bread
(2) butter
(3) ham
(4) corn
A (1), (3), (2), (4)
B (1), (4), (3), (2)
C (3), (1), (4), (2)
D (4), (1), (2), (3)

16. Which of the following statements about the control set-up of an experiment is/are correct?
(1) It is identical to the experimental set-up except that the factor under investigation is absent.
(2) It is to ensure the result of the experiment is due to the factor under investigation.
(3) Not all the investigations require a control set-up.
A (1) only
B (1) and (2) only
C (2) and (3) only
D (1), (2) and (3)

17. If the result of an experiment does not match the prediction which is made based on the hypothesis,
that means
A the experiment does not need a hypothesis.
B a conclusion cannot be drawn.
C assumptions have to be made.
D the hypothesis is not supported.

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Directions: The following two questions refer to the diagram below, which shows the fluid mosaic
model of the cell membrane.

P Q

18. Which of the following is/are the role(s) of structure X?

(1) cell recognition
(2) carrying polar substances across the cell membrane
(3) allowing non-polar substances to move across the cell membrane
A (1) only
B (2) only
C (1) and (2) only
D (2) and (3) only

19. Which of the following correctly shows the paths through which water and fatty acid molecules
move across the cell membrane?
Water Fatty acid
A path P path P
B path P path Q
C path Q path P
D path Q path Q

20. According to the fluid mosaic model of cell membrane, which of the following statements about
the phospholipid molecules in the cell membrane is correct?
A They have hydrophilic tails pointing to the interior of the membrane.
B They are arranged in a bilayer, with membrane proteins covering the surfaces of the membrane.
C They are free to depart from the membrane and dissolve in the surrounding aqueous solution.
D They can move laterally along the plane of the membrane.

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21. Which of the following nutrients cause the yellowing of leaves on a plant when there is a
deficiency ?
(1) nitrogen
(2) phosphorus
(3) magnesium
A (1) and (2) only
B (1) and (3) only
C (2) and (3) only
D (1), (2) and (3) only

22. Which of the following is/are the adaptive feature(s) of a dicotyledonous leaf for facilitate
photosynthesis?
(1) Palisade mesophyll cells are loosely packed.
(2) Spongy mesophyll cells consist of chloroplasts.
(3) A layer of cuticle on the surface of epidermis is transparent.
A (1) only
B (1) and (2) only
C (2) and (3) only
D (1), (2) and (3) only

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23. Directions: Questions 23 and 24 refer to the diagram below, which shows an experimental set-up
used to investigate the effect of light intensity on the gas exchange of an aquatic plant.

oxygen sensor connected

to data logger
interface
sodium
hydrogencarbonate
lamp
solution light sensor

aquatic plant

The graph below shows the results obtained from the experimental set-up. The results obtained
from a control set-up are also shown.

experimental set-up
10

oxygen
control set-up
concentration 6
(arbitrary unit)

0 100 200 300 400 500 600

light intensity (arbitrary unit)

Which of the following diagrams shows the control set-up used in the experiment?

sodium
A hydrogencarbonate
solution

aluminium foil

B water

sodium
C hydrogencarbonate
solution

sodium
hydrogencarbonate
solution

D water bath
at 30°C

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24. Which of the following can be deduced from the results obtained?
(1) The plant reaches the compensation point when light intensity is 470 arbitrary units.
(2) The plant does not carry out photosynthesis when light intensity is lower than 470 arbitrary
units.
(3) When light intensity is higher than 100 arbitrary units, there is net production of food by the
plant.
A (1) only
B (2) only
C (1) and (3) only
D (2) and (3) only

25. The photomicrograph below shows the appearance of part of the upper epidermis of a leaf of a
floating plant.

Which of the following statements about cell X and cell Y is/are correct?
(1) Cell X contains chloroplasts but cell Y does not.
(2) The thickness of the cell wall of cell X is uneven while that of cell Y is even.
(3) Both cell X and cell Y are absent in the lower epidermis of the leaf.
A (1) only
B (1) and (2) only
C (2) and (3) only
D (1), (2) and (3)

26. A student investigated the stomatal distribution on the leaves of four plant species
(A, B, C and D) living in different habitats. The results are shown in the table below.
Species Area of leaf sample (mm2) Number of stomata on upper
epidermis
A 401 88
B 310 78
C 520 61
D 238 110
Which of the following plant species has the highest stomata density?
A Species A
B Species B
C Species C
D Species D

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27. Which of the following methods are involved to provide mainly support to the woody stem?
(1) Mechanical support of the thick-walled cells.

(2) Turgidity of thin-walled cells

(3) Hydrostatic pressure of pith

A (1) only
B (2) only
C (1) and (2) only
D (1), (2) and (3)

Directions: Questions 28 to 30 refer to the diagram below, which shows an experiment in progess.

Initial weight of the whole set-up = 600g

Final weight of the whole set-up = 570 g
Initial reading of the graduated tube = 3cm3
Final reading of the graduated tube = 39cm3
Density of water = 1gcm-3
Duration of the experiment = 3 hours

28. The rate of transpiration of the plant is

A 10ghr-1
B 15ghr-
C 20ghr-1
D 25ghr-1

29. The amount of water absorbed by the plant is

A 1 cm3
B 3 cm3
C 24 cm3
D 36 cm3

30. The amount of water retained by the plant is

A 1g
B 2g
C 3g
D 6g

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31. The diagram below shows a cell at a certain stage of cell division (only two pairs of homologous
chromosomes are shown).

Which of the following statements about the type of cell division shown in the diagram is correct?
A The daughter cells formed cannot undergo further cell divisions.
B Four daughter cells will be produced after the cell division is completed.
C The number of chromosomes in each daughter cell is equal to that in the parent cell.
D The daughter nuclei are not identical.

32. The diagrams below show different stages of cell division.

(1) (2)

(3) (4)

Crossing over is most likely to occur at

A stage (1).
B stage (2).
C stages (2) and (3).
D stages (3) and (4).

33. The diagram below shows the chromosomes of an animal cell at the prophase of mitosis.

What are the diploid number and haploid number of this animal?
Diploid number Haploid number
A 4 2
B 8 4
C 8 16
D 16 8

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34. The following three questions refer to the diagrams below, which show the different stages of
mitosis in animal cells.

Stage P Stage Q Stage R Stage S

The correct order for the four stages of mitosis shown above is
A P, Q, R, S
B S, P, Q, R
C R, S, P, Q
D Q, R, S, P

35. What is happening at R?

A The chromosomes thicken and shorten.
B The chromatids start to separate from each other.
C The chromosomes line up in the middle of the cell.
D The chromatids move to the opposite ends of the cell.

36. Which of the following are haploid cells?

(1) Muscle cells
(2) Ovum
(3) Nerve cells
(4) Sperms
A (1) and (2) only
B (2) and (4) only
C (1) and (3) only
D (3) and (4) only

37. The diagram shows the transverse section of a lemon.

X
Y

Which of the following structures are genetically identical?

(1) structure X
(2) structure Y
(3) structure Z
A (1) and (2) only
B (1) and (3) only
C (2) and (3) only
D (1), (2) and (3)

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38. The photograph below shows a daffodil plant.

Which of the following statements about structure X is/are correct?

(1) It is a rhizome.
(2) It stores a lot of food.
(3) It contains buds and seeds.
A (2) only
B (1) and (2) only
C (2) and (3) only
D (1), (2) and (3)

39. The diagram below shows the longitudinal section of a ripe fruit:

Structure 2 is absent at position X because

A there was only one ovule in the ovary.
B only one of the ovules fertilized could develop.
C only one ovule could be fertilized in each ovary.
D the ovule there has not been fertilized.

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40. The photomicrograph below shows the transverse section of a part of a lily flower.

Y
(20)

Which of the following statements about structures X and Y is/are correct?

(1) X splits open when Y is ready for dispersal.
(2) Y is the male gamete of the plant.
(3) Y fuses with the female gamete when it lands on the stigma of the same species.
A (1) only
B (1) and (2) only
C (2) and (3) only
D (1), (2) and (3)

— End of Section A —

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