2.

Materials and Methods

2.1. Identification of plant material. Ixora
coccinea leaves or locally known as
2.4. Determination of tannin content of I.
santan were obtained from Antipolo City
coccinea. For the analysis of the tannins
(Rizal, Philippines). The leaves were
of the extracted sample the following
collected by removing the flowers and
tests were performed: Ferric chloride
washed by using tap water.
test, Catechin test, and Iodine test.
2.2. Chemicals. Ethanol (95%), Ferric
2.4.1. Ferric chloride test. A quantity (2
chloride (5%), and Hydrochloric acid.
ml) of plant sample were placed into a
2.3. Preparation of Crude Extracts. At test tube and 0.6 ml of ferric chloride
room temperature the leaves of I. was added. A change of color into dark
coccinea were air-dried for several days blue indicates hydrolysable tannins,
and then ground to a fine powder. About while a greenish black solution indicates
8.34 g of powdered plant sample were non-hydrolysable tannins.
macerated in 100 ml ethanol (95%) for
2.4.2. Iodine test. A quantity (2 ml) of
72 hours at room temperature. The
plant sample were put into a test tube
extracted sample were filtered for a few
and 0.4 ml of Povidone-iodine was
times until the solvent was separated.
added. A precipitation and a change of
The final weight of the filtered sample
color into brown solution indicates a
was 50 ml and was stored for further
presence of tannins.
use.
2.4.3. Catechin test. A matchstick was
dipped into a plant sample and then was
dried before dipping into hydrochloric
acid. A change of color into pink or red
[Grab your reader’s attention with a
of the matchstick indicates the presence
great quote from the document or use this
of tannins.
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Figure 1. Procedure in the extraction of

crude extract in Ixora coccinea.