Professional Documents
Culture Documents
The main purpose of this research is to synthesize and evaluate the wound
healing activities of hydratable keratin solid powder from human hair and its in
vitro application on mice (Mus musculus).
The researchers will gather twenty-seven (27) male mice (M. musculus)
at the Yenlen Pet Shop at Libertad, Bacolod City. The human hair will be
obtained from the cuttings of barber and beauty shops in, Bacolod City. Human
hair is a preferred source of keratinous material due to its availability and it is less
prone to cause allergic reactions to a human when tested in clinical trials.
The extraction of hydratable keratin from human hair and the evaluation of its
physical and chemical properties which involves color, odor, texture, solubility,
pH and flammability will be conducted at the Herbanext Laboratory at Brgy.
Taloc, Bago City and Negros Prawn Producers Diagnostic and Analytical
Laboratory. While the in vitro application of the generated hydratable keratin on
mice will be performed at the Senior High School Laboratory of Negros
Occidental High School, Bacolod City. Additionally, the wound of the mice treated
with hydratable keratin will be assessed in terms of its percent wound
contraction, epithelialization, the degree of fibrosis, number of fibroblasts and
fibrocytes, and vascular proliferation. The whole research study will be carried
out in a span of 1month starting from September 10- October 10, 2018.
Data gathered will be analyzed using Mean and Standard Deviation as
descriptive tools and Analysis of Variance (ANOVA) at α= 0.5 as an inferential
tool of the study.
This study can be expected to delimit in the number of its set-ups for
which it will only encompass three (3) set-ups namely the Set-up A which will be
the wound dressing with hydratable keratin, the Set-up B which will be the
commercial wound dressing and the Set-up C which will be the untreated group.
The parameters such as the dermal toxicity, exudate absorption and acute
irritation will also be delimited. Moreover, delimitations can commence in the
manner of sampling that will be used in distinguishing and opting for distinct
journal articles as the main sources of data.
INCLUSIONS:
NOTE: Pwede man paragraph form nga daw amo ni, and actually ang
amo n nga example is an excerpt about influence of internet connection
sa academic performance, may makwa gid kamo di
Note: In your scope and limitations, indicate which sections of the ME are
included...etc.
(5) Definition of Terms
(b) Operational Definition- ari you make your own definition based sa use
sang “particular word” sang aton nga study
This portion sets forth the distinct variables as well as the crucial terms
employed in the study. Also, the conceptual and operational definitions are
imparted.
Absorbency. Conceptually defined as the mean volume of water absorbed
by board materials and water-soaked paper. The ability of a substance to
take in water (Taylor, 2014). Operationally, it is one of the parameters that
is going to be used in order to know the ability or capacity of the dressing
to soak up exudates.
Biocompatibility. Conceptually defined as the competence of a
material to execute in a precise operation with an applicable host
(Linsmeier, et al., 2013). Operationally, the ability of the hydratable keratin
to be integrated to the dressing.
Biosynthesis. Conceptually defined as the creation of a complex
chemical compound typically including enzymes to prompt the reaction
and power origin like the Adenosine Triphosphate (ATP) derived from
common precursors in an existing organism (Irish Institute for
Biosynthesis, 2017).
Degree of Fibrosis. Conceptually defined as the degree of fibrous
connective tissue growth in response to injury (Robertson, 2014).
Operationally, one of the factors to be deliberate in order to determine the
effectiveness of the wound dressing.
Epithelialization. Conceptually defined as a prime constituent of
wound healing utilized as a delineating parameter of a prosperous closure
of a wound (Pastar, I., et al., 2014). Operationally, it is one of the
determining factors in finding out the effectiveness of the dressing.
Exudates. Conceptually defined as a protein-riched fluid that
exudes out of a blood vessel as a result of inflammation (Center, 2013).
Operationally, the absorption of exudates will be used as a parameter to
determine the quality of the wound dressing from hydratable keratin
powder in terms of healing.
Fibroblast. Conceptually defined as cells accountable for the yield
of the building blocks of the extracellular matrix which are collagen,
glycosaminoglycans, and proteoglycans (Williams, 2013). Operationally,
one of the parameters to be measured to calculate the efficiency of the
wound dressing.
NOTE: Define all key words here EXCEPT amidst and other
prepositions and “the” in the title of the study.
Also, ill add terms from time-to-time ha, kay medyo early stage
palang ni ka study ta.