HEMATOLOGY LABORATORY REVIEWER

Instructor: Charlene Ann Mesiona, RMT

By: Rashlea M. Omar BSMT 3
-plain tube (w/o heparin)
-used for anticoagulated bllood
Hematology Laboratory: Activity No. 1
 NOTE:
Materials used in Hematology -2/3 of the tube should be filled before
sealing
MATERIALS
• Capillary tube
• Cover slide COVER SLIDE (COVER GLASS)
• Improvised suction device
• Westergren tube
• Forceps
• Pasteur pipette
• Wintrobe tube
• Glass slide
• Sahli pipette
REAGENTS
• Wright’s stain
• New Methylene Blue
• Diluting Fluids IMPROVISED SUCTION DEVICE
RBC
WBC
Platelets
EQUIPMENT
• Hematocrit centrifuge
• Centrifuge
• Automatic pipette
• WBC Differential Counter
• Microscope
CAPILLARY TUBE
WESTERGREN TUBE

 Appoximately 70mm long with an inner

bore of 1-1.55mmHolds 0.05mL of
blood
 RED BAND
-with heparin
-used for non-anticoagulated whole
blood
 BLUE BAND
 HEMATOLOGY LABORATORY REVIEWER
Instructor: Charlene Ann Mesiona, RMT
By: Rashlea M. Omar BSMT 3

PASTEUR PIPETTE

WINTROBE TUBE

GLASS SLIDE
WESTERGREN TUBE WINTROBE TUBE

200 mm length x 5 mm 100 mm length x 2.5

diameter mm diameter

Anticoagulant: EDTA or Anticoagulant: Double

0.109M Trisodium Citrate Oxalate

distance of red cell is distance of red cell

SAHLI PIPETTE
measured at the end of fallen after one hour
one hour

no correction necessary for needs corrections for

hematocrit hematocrit

FORCEPS

COPLIN JARS
 HEMATOLOGY LABORATORY REVIEWER
Instructor: Charlene Ann Mesiona, RMT
By: Rashlea M. Omar BSMT 3

RBC PIPETTE WBC PIPETTE

SPREADER SLIDE
Bead color RED WHITE

Dilution 1: 100 - 1: 1: 10 - 1: 100

range 1000

Outstandi 0.5, 1.0, 101. 0.5, 1.0, 11.0

ng marks 0

Principal • RBC count • WBC count

uses • Direct platelet • Eosinophil count
RBC THOMA PIPETTE count • Cell counts in
• WBC count in CSF and other
leukemia body fluids

STAINING RACK

WBC THOMA PIPETTE

HEMATOCRIT READER
 HEMATOLOGY LABORATORY REVIEWER
Instructor: Charlene Ann Mesiona, RMT
By: Rashlea M. Omar BSMT 3
ANTICOAGULANTS

TEST TUBE RACK

OXALATE

• Inversion: 5-10X
• Actions: Combines with calcium to form
insolube salts – calcium oxalate
• Forms: Potassium Oxalate, Ammonium
Oxalate, Double Oxalate
• Tests: ESR (Wintrobe Method

EDTA (Ethylenediamine Tetraacetic Acid)

• Inversion: 8-10x
HEMOCYTOMETER • Actions: Chelates Calcium
• Forms:
o -Sequestrene (K2/K3 EDTA) -
LIQUID FORM
o -Versene (Na EDTA) – SOLID
FORM
• Tests: Routine Hematology, Platelet Ct.
• NOTE!!!!!
-EDTA artificially increases potassium =
25 mEq/L
TYPES
CITRATE
1. Open (Burker type)
2. Closed (Tomas- Zeiss) • Inversion: 3-4x
MODELS • Actions: Binds with Calcium form soluble
1. Levy complexes
2. Levy Hauser • Forms:
3. Spencer Bright line - 3.2% (0.105 M) Sodium Citrate –
RULINGS light blue top
1. Fuchs- Rosenthal
2. Neubauer - 3.8% (0.129 M) Sodium Citrate –
3. Improved Neubauer disodium/trisodium; black;
Westgren method
VACUTAINER TUBES • Tests: Collection for transfusion,
Coagulation studies, ESR

HEPARIN
• Inversion: 8-10x
• Actions: Accelerates Antithrombin III,
neutralizes thrombin and prevents fibrin
formation
• Forms:
 HEMATOLOGY LABORATORY REVIEWER
Instructor: Charlene Ann Mesiona, RMT
By: Rashlea M. Omar BSMT 3
- SAME WITH EDTA PLATELET DILUTING FLUID
• Tests: Osmotic Fragility Test, Blood Gas • REESE- ECKER FLUID
Analysis, Exchange Transfusion

FLUORIDE EQUIPMENT
• Inversion: 8-10x
• Actions: Formed weakly dissociated calcium
component HEMATOCRIT CENTRIFUGE
• Forms:
- Na Fluoride
- K Fluoride
• Tests: Glucose Determination

REAGENTS

Wright's Stain
• Acid dye (Eosin) + Basic
dye (Methylene blue) • Calibration:
• Should be filtered -Stopwatch for accuracy
before use -Tachometer for speed
• METALLIC SHEEN
• Speed: 10,000- 15,000 RPM for 5 minutes
(“green scum”) should
• NOTE:
appear on the slide if
mixing is correct
-Centrifuge should reach the maximum
speed within 30 seconds

NEW METHYLENE BLUE

• Supravital stain CENTRIFUGE

• Reticulocyte
staining
• Stains RNA (deep
blue color)

RBC diluting fluid

• FLUID, TOISSON'S FLUID, BETHEL'S
FLUID ISOTONIC
• E.g. DACIE'S FORMOL CITRATE,
HAYEM,S FLUID, GOWER'S
WBC diluting fluid
• should contain GLACIAL ACETIC ACID
• E.g. TURKS DILUTING FLUID, 1% HCl, 2-
3% GLACIAL ACETIC ACID
 HEMATOLOGY LABORATORY REVIEWER
Instructor: Charlene Ann Mesiona, RMT
By: Rashlea M. Omar BSMT 3

AUTOMATIC PIPETTE

WBC DIFFERENTIAL COUNTER

MICROSCOPE