HEMATOLOGY LABORATORY QUIZZES

COMPILED BY RASHLEA OMAR BSMT 3RD YEAR

10. DIKO MAKITA QUESTION PERO ANG

ANSWER KAY: ORANGE
QUIZ NO. 1 11. Optimal concentration of EDTA
 1.5 mg per ml of blood
1. What is the action of OXALATE as an
12. What is the mode of action of EDTA
anticoagulant?
 CHELATES CALCIUM
 COMBINES WITHCALCIUM 13. MATCH COLUMN A TO B
TO FORM AN INSOLUBLE
a) LAVENDER GLASS - K3 EDTA
SALT
b) LAVENDER PLASTIC - K2EDTA
2. What is the insoluble salt that
c) WHITE - MOLECULAR
becomes the final product of
DIAGNOSTIC TESTING
Oxalate?
d) TAN - LEAD TESTING
 CALCIUM OXALATE e) YELLOW (SPS) -
3. The white-anticoagulant tube is MIROBIOLOGY CULTURE
used for what test? f) DARK BLUE - TOXICOLOGY
 MOLECULAR DIAGNOSTIC g) PINK - VIRAL LEAD TESTING
TESTING FOR PLASMA h) LIGHT BLUE - COAGULATION
4. What is the optimal concentration of STUDIES
fluoride? i) YELLOW (ACD) - DNA
 15-20U/Ml TESTING
5. Potassium oxalate inhibits what j) LIGHT GRAY - PRESERVE
enzyme? GLUCOSE FOR 3-5DAYS
 ENOLASE 14. ANTICOAGULANT=TUBE COLOR
6. The Black anti-coagulated tube is a) HEPARIN= GREEN
used in what laboratory test? b) SODIUM
 ERYTHROCYTE POLYANETHOLSULFATE=
SEDIMENTATION RATE YELLOW
7. The green antic-coagulated tube c) SODIUM CITRATE= LIGHT
could contain either sodium heparin BLUE
or lithium heparin, identify which of d) BUFFRED SC= BLACK
the two will be used for determining e) EDTA W/ GEL SPERATOR=
the lithium level? WHITE
 SODIUM HEPARIN f) CLOT ACTIVATOR=
8. How many inversions should be RED/GRAY
done for NON-ADDITIVE GLASS g) SEPARATOR GEL= GOLD
TUBE? h) EDTA= LAVENDER
9. What component (anti- i) OXALATE= GRAY
coagulant/additive) of Yellow tube 15. What are the 3 forms of OXALATE?
that could use HLA Phenotying?  POTASSIUM OXALATE
 ACID CITRATE DEXTROSE  AMMONIUM OXALATE
 HEMATOLOGY LABORATORY QUIZZES
COMPILED BY RASHLEA OMAR BSMT 3RD YEAR

 DOUBLE OXALATE 24. Anticoagulant that is used for

Routine Hematology?
 EDTA
16. What are the 2 forms of citrate and 25. Another name of K2/K3 EDTA
its molarity?  SEQUESTRENE
 3.2% (0.105 M)
 3.8% (0.129 M)
17. What is the BLOOD TO
ANTICOAGULANT RATIO of Sodium QUIZ NO. 2
Citrate when used in ESR? 1. What is the chromatin pattern of
 4:1 basophils?
18. What is the BLOOD TO  CLUMPED
ANTICOAGULANT RATIO of sodium 2. What is the smear used in looking for
citrate in coagulation studies? malarial parasite?
 9:1  THICK SMEAR
19. What happens if there is an 3. Fine azure granules of monocytes are
also called
increased citrate in patients with
 AZURE DUST
Polycythemia Vera?
4. What are the factors that determine the
 CITRATE WILL BIND WITH thickness of the smear
CALCIUM IN THE  PRESSURE
COAGULATION REAGENT  ANGLE
RESULTING IN  SIZE
COAGULATION TIME  SPREAD
20. Known as the naturally occurring 5. The components of romanowsky stain
anticoagulant are_______ in nature
 HEPARIN  POLYCHROMATIC
21. What is the main disadvantage of 6. What are the 3 buffer solutions that can
HEPARIN in Blood Stained Films? used in staining Blood smear?
 CAUSES BLUISH  0.05 M SODIUM PHOSPHATE
(PH 6.4)
COLORATION OF THE
 AGED DISTILLED WATER (PH 6.4
BACKGROUND ON BLOOD
– 6.8)
FILM
 SORENSON’S PBS (PH 7.2)
22. Fluoride prevents glycolysis when 7. What is the N:C ration of monocyte?
bind with what mineral?  1 (ONE)
 MAGNESIUM 8. Too alkaline wright stain may be due to?
23. This anticoagulant is both  Thick Blood Smears
anticoagulant and additive, what is  Prolonged Staining
this?  Insufficient Washing
 FLUORIDE  Too alkaline pH of stain, buffer
or water
 HEMATOLOGY LABORATORY QUIZZES
COMPILED BY RASHLEA OMAR BSMT 3RD YEAR

 New stain solution which has  LARGE GRANULAR

not stood for 2-3 weeks LYMPHOCYTE
 May be too basic 19. What does Ph stands? What does it
9. This cell is used to clean up dead cells mean?
 MONOCYTE  POWER OF HYDROGEN
10. Give at least 3 properties of an ideal 20. What cell helps in producing antibodies?
blood smear  LYMPHOCYTES
 Gradual transition from thick to 21. What are the 3 types of lymphocytes?
thin  T LYMPHOCYTE
 Smear must occupy 2/3 – ¾ of  B LYMPHOCYTE
the slide  NULL LYMPHOCYTE
 Margin-free 22. What is the ideal ph in blood smear
 With feather edge and has staining?
“rainbow” appearance  PH 6.3 – 7.3
 No overlapping of cells 23. What method uses glass slide and cover
 Smear surface must be without slip in making blood smear?
scratches, free from ridges and  BEACOM’S
waves and holes 24. What is the main function of Glycerine in
11. Basophils is involved in what common Wright stain solution?
type of allergic reactions?  STABILIZER
 HYPERSENSITIVITY 25. What is the most abundant type of
12. Give at least 2 remedies to correct too WBC?
acidic wright stain  NEUTROPHIL
 Prolonged the staining time 26. What are the 4 criteria for WBC
 Check the pH of stain & buffer Classification and Identification?
 Correct with alkali if necessary  Cell Size
 Shorten the buffering time  Nucleus: Cytoplasm Ratio
13. What is the preferred stain use in  Cytoplasm Characteristics
staining blood smear?  Nuclear Characteristics
 MAY GRUNWALD GIEMSA STAIN 27. What are the 4 methods in drying a
14. Half-life of eosinophil’s before entering smear?
the tissue  Air dry
 18 HOURS  Use of low flame
15. Monocyte chromatin has a ground glass  Use of Oven
appearance  Immersion of Methyl Alcohol
 FALSE 28. Give the 3 components of wright stain
16. What is the size of a glass slide in solution and its corresponding amount
millimetre needed to make the solution
 75mm x 25 mm  Wright Stain Powder: 3g
17. What sample do we use in making blood  Glycerine (Stabilizer): 30 mL
smear when patient WBC count is <1.0 x  Absolute Methyl Alcohol
10’9/L? (Fixative) : 1000mL
 BUFFY COAT
18. Another name for large lymphocyte?
 HEMATOLOGY LABORATORY QUIZZES
COMPILED BY RASHLEA OMAR BSMT 3RD YEAR

29. What is the angle used in making blood  30 cm

smear? 40. Lobes of segmented neutrophils is
 30 TO 45 DEGREES connected by what filament?
30. What method uses 2 cover slips in  THREADLIKE FILAMENTS
making blood smear?
 ERLICHS
31. What are the 2 components of QUIZ NO. 3
Romanowsky stain?
 EOSIN Y 1. The doctor requested a test for a patient
 METHYLENE BLUE suspected for coagulation defects and
32. What are the 3A’s to remember in bacterial infection. What should be the
making PBS? collection tube?
 APPROACH  BLUE TOP/ YELLOW TOP
 ADHESION 2. She wants to monitor potassium and
 ADVANCEMENT sugar, then after that she asked the
33. How many nucleoli are present in Medtech if they can extract another
monocyte? one for CBC. What should be the color
 NONE of the collection tube be used?
34. What the ideal anticoagulant used in  RED TOP/ LAVENDER TOP
making blood smear? 3. She was diagnosed of blood infection.
 K2 EDTA The doctor requested some tests for
35. What is the shape of a monocyte her. What should be the color of the
nucleus? collection tube?
 HORSESHOE SHAPED  BLACK TOP/YELLOW TOP
36. What the 3 advantages of a blood 4. Requested test for cross matching and
smear? bacterial culture. What should be the
 Can be properly labelled color of the collection tube?
 Can be properly stored  PINK TOP/ YELLOW TOP
 Easy to handle and prepare 5. Test for FBS and electrolytes. What
37. Give at least 2 ways of scanning smears? should be the color of the collection
 TWO FIELD MENDER tube?
 FOUR FIELD MENDER  RED TOP
 CRENELLATION 6. Requested for creatinine and CBC for
TECHNIQUE/BATTLEMENT her dialysis patient. What should be
TRACK SCAN PATTERN the color of the collection tube?
 EXAGGERATED BATTLEMENT  RED TOP/ LAVENDER TOP
METHOD 7. Request a test for WBC count and APTT.
 STRIP METHOD What should be the color of the
38. What is the smear used to identify and collection tube?
classify malarial parasite?  BLUE TOP/ LAVENDER TOP
 THIN SMEAR 8. Ordered a series of test for Routine
39. What is the ideal distance of a cold blast Chemistry, Hematology, ABG, and she
drier in drying blood smear? is also advised for Bacterial culture.
 HEMATOLOGY LABORATORY QUIZZES
COMPILED BY RASHLEA OMAR BSMT 3RD YEAR

What anticoagulant used for the WBC 16. It is an anticoagulant that act as both
Diff count of Mr Dellosa? preservative and anticoagulant.
 EDTA  FLUORIDE
9. Ordered a series of test for Routine 17. The doctor ordered to run some tests
Chemistry, Hematology, ABG, and she for a 35 years old man who has a
is also advised for Bacterial culture. . electrolyte imbalance and has a
What should be the color of the bacterial infection?
collection tube?  RED TOP/ YELLOW TO
 YELLOW, GOLD, GREEN, 18. DENGUE PATIENT
LAVENDER  RED TOP/ LAVENDER TOP
10. RN, Assigned to perform arterial 19. Baby boy was born Philippine General
puncture for a patient in the Emergency Hospital and needs a blood
room. What is the appropriate transfusion, suddenly the doctor also
anticoagulant to be used? noticed that the baby has a Fever the
 NONE OF THE ABOVE doctor call the laboratory to run
11. Spikey Alga, A registered medical some test. What tubes should be the
technologist, was assigned to draw Medtech on duty prepare?
blood from 1 day old baby for New-  LAVENDER TOP/ PINK TOP/
born Screening. What will she do? YELLOW TOP
 SHE IS ALLOWED TOGET A SAMPLE 20. ???
FOR NEW-BORN SCREENING
PROIVIDED TAT SHE WILL
PERFORM SKIN PUNCTURE.
12. Dr Fawawen Uks will perform an
arterial puncture for patient X. What
is the gauge of the needle to be used
and what is the angle for the
puncture?
 23g, 45 degrees
13. A patient was requested for K+
measurement by his physician. What
appropriate anticoagulant to be
used?
 HEPARIN
14. A patient was required for HB1AC test.
What appropriate anticoagulant to be
used?
 EDTA
15. What is the function of the
Anticoagulant used for Gray top?
 PREVENTS GLYCOLYSIS BY
FORMING A COMPLEX WITH
MG2+