HEMA LEC QUIZ LAB EVAL PRIMARY HEMOSTASIS  Shortened

 Prolonged
1. The process of blood clotting = COAGULATION 11. What is inhibited by aspirin?
 Cyclooxygenase
2. Platelet Rich Plasma (PRP)  Endoperoxidase
1. Used for platelet studies  Lipooxygenase
2. Stored at room temperature
3. Used for coagulation studies (X) 12. What is the most potent penicillin that can: affect platelet
4. Derived from whole blood function?
 All choices are incorrect  Carbapanem
 3 of the choices are correct  All are correct
 One of the choice is correct  Carbenicillin
 2 of the choices are correct All choices are correct
13. Patients with erythema have what coagulation result?
3. What is released if the specimen is hemolyzed?  Prolonged
1. ADP  Shortened
2. Collagen
 1 is correct 14. What is the 3rd tube to be filled in ETS order of draw?
 Both are incorrect  No answer
 2 is correct  EDTA
 Both are correct  Used for platelet count
 All are correct
4. This method allows visualization of platelet morphology
 Fonio's method 15. Associated with loss of carbon dioxide that deteriorates
 Gives correct platelet count clotting factors if the pH is decreased
 All correct  True
 NoNoanswer
answer  False (increased)

5. Platelet count depends on its electrical resistance 16. 2-3 standardized punctures are made on the forearm
 No answer under constant pressure what method is being asked? (please
 Indirect method use capital letters)
 Direct method IVY’S METHOD
 Automated method ????
17. Bleeding time is sensitive and dependent on platelet plug
6. What grading it is if there are multiple petechiae over formation
anterior and back of the hand?  True
 2+  False
 3+
 1+ 18. Standardized puncture is made on the earlobe (please use
 4+ capital letters)
DUKE’S METHOD
7. What method uses castor oil?
 Hirshboek Method 19. The original test for platelet function test (caps
 No Answer lock no abbreviation)
 Castor oil BLEEDING TIME TEST
 All are correct
20. Bleeding time was first described by: (caps lock no
8. What anticoagulant is used for in vitro platelet adhesion abbreviation)
tests? William W. Duke
 EDTA
 Oxalate 21. Acquired Platelet defects are associated with:
 Citrate 1. Liver disease
2. MDS
9. What is inhibited by the EDTA? 3. Myeloma
 Antithrombin 4. Dysplastic plts.
 Thrombin  1 and 2
 3 and 4
10. What happens to the coagulation result if the specimen is  2 AND 3
hemolyzed?  All choices are correct

The concentration of sodium citrate: 3.2%

 c. Hemolysis has no effect
22. To avoid cryoprecipitation of vWF specimen may be d. The specimen is clotted
frozen and thawed.  A
 True  B
 False  C
 D
SET 2
1. What color of capillary tube should be used for capillary 11. Most coagulation testing must be performed on PPP,
tube test? which is plasma with a platelet count less than:
 Blue
Blue band
band (not sure) a. 1000/ul
 Heparinized
Heparinized b. 10,000/ul
 No answer c. 100,000/ul
 Red band d. 1,000,000/uL
 A
2. Most specimens for whole-blood aggregometry are  B
mixed with normal saline before testing  C
 1:2  D
 1:100
 1:1 12. Aspirin irreversibly acetylates the platelet enzyme
 1:3 cyclooxygenase at the at what Position
 No correct answer
3. Stasis results in the local accumulation of coagulation  Acetyl group
factor VIII and VII  Serine
 True  ADP
 False (vWF and Factor VIII)
13. UFH is administered intravenously to provide immediate
4. Arachidonic acid is readily oxidized and must be stored at control of coagulation
20°C in the dark.  True
 True  False
 False (-20°C) 14. Sodium citrate inhibits all coagulation factors
 True
5. Primary aggregation involves shape change  False
 True
 False 15. Plain glass tubes will activate
 Extrinsic pathway
6. Platelet aggregometry helps determine the cause of  Both are correct
thrombocytopenic mucocutaneous bleeding  No correct answer
 True  Intrinsic pathway
 False (nonthrombocytopenic mucocutaneous
bleeding) 16. Coagulation factor that will break down at temperature
above 37 degrees C. FACTOR V & FACTOR VIII
7. ADP is the most commonly used agonist 17. Recalcifying agent for Factor III assay. Calcium
 True chloride
 False 18. The concentration of sodium citrate is 3.2%
19. Centrifugal force and time to obtain PPP
8. If a coagulation specimen collection tube is underfilled  1500-2000 rpm for 10-15 mins
test result will be prolonged.  2000-2500 rpm 10-15 minutes
 True  20000-2500 rpm for 10 mins
 False  No correct answer

9. ADP used in platelet aggregometry to detect VWD?

 True
 False (Ristocetin)

10. What is the effect of hemolysis on a hemostasis

specimen
a. In vitro platelet and coagulation activation occur
b. The specimen is icteric or lipemic
Thrombin time is a tests for coagulation factor A modification of plasma clotting time is called
deficiencies. Reference value is 5-10 minutes activated recalcification time. It employs use of
.1ml 0.25 M calcium chloriode
Both are incorrect
1st is correct 2nd incorrect
What factor deficiencies can lead to a
prolonged PT Screening tests used to evaluate extrinsic
pathway
II
PT
X
Best considered as a test of platelet
V phospholipid activity
Prothrombin PCT
Fibrinogen Partial thromboplastin time is also called APTT
ABCDE False
Causes of abnormal prothrombin time Heparin may prolong the result of PT
Vit K deficiency TRUE
Liver disease Test for fibrinolysis include:
Factor deficiency Sagot nila: Quantitative D-Dimer immunoassay,
All are correct Fibrin degradation product immunoassay,
plasminogen chromogenic substrate assay,
Coagulation factor assay are used to detect and Tissue plasminogen activator assay,
measure coagulation factor deficiencies Plasminogen activator inhibitor-1 assay
True Pwedeng sagot: Whole blood clot lysis, Euglobin
Assay for increased in fibrin monomers lysis time, Protamine sulfate turbidity test,
Latex D dimer assay, Fibrin degradation product
PST
PSTT (PROTAMINE SULFATE TURBIDITY TEST) immunoassay, Tissue plasminogen activator
Fibrinogen is usually converted intro fibrin assay, Quantitative assay of fibrin-fibrinogen
which is quantified by gravimetric, degradation products
nephelometric and precipitation method Measures the amound of time it takes thrombin
True to convert fibrinogen to a fibrin clot

Clotting time
The apt is usedful procedure for the fallowing
except Incubation of the plasma at 37 degree C for
FXII more than 5 minutes may cause a decrease of

FV
PRESENCE OF INHIBITORS
Test for factor X or V deficiency
HEPARIN THERAPY
Stypven time
FVII
One of the causes of abnormal thrombin time is
presence of abnormal fibrinogen

True

The D dimer assay is essential for ruling out

venous thromboembolic disease

False

Prothrombin time measures intrinsic and

common pathway; more sensitive method than
the coagulation time of whole blood

Both incorrect

The 2 physiologic human plasminogen

Tissue plasminogen activator and urokinase

1. The rvvt is used to detect deficiencies in: ALL ARE CORRECT

2. If ppt is abnormal the stypven time may not need to perform: FALSE

3. D-dimer is produced from cross-linked fibrin: TRUE

4. If the prothrombin time is normal the ST need not be performed: TRUE

5. Platelet factor 3 is released when there is platelet activation this it will

participates in: THROMBIN FORMATION

6. All coagulation studies are carried out at what temperature: 37

DEGREES CELSIUS

7. Prothrombin time is useful in screening procedure for extrinsic to include

defects in factor II and V: FALSE

8. The activated protein C inhibits factor V and factor VII thus it will
APTT: PROLONGED

9. Warfarin suppresses the production of factor VII: TRUE

10. Decreased thrombin production may reduce the activation of TAFI,

resulting in decreased fibrinolysis that contributes to more bleeding: BOTH
CORRECT

11. Incubation at more than 5 mins may cause a decreased in factor V and
factor VII causing: SHORTENED THE CT RESULT

12. The PTT is useful screening procedure for extrinsic coagulation

including the common pathway: TRUE

13. Prothrombin time: 1 is correct

14. (I) Lee and White clotting time was used as a screening test to measure
all stage in the extrinsic pathway. (II) And used to monitor Coumadin therapy.:
Both are incorrect
15. The ISI for each thromboplastin is published by the manufacturer: TRUE

16. PTT is insensitive to factor II: FALSE

17. The PT is least sensitive to factor II: TRUE

18. The plasma recalcification time varies according to the number of

platelets present in serum: FALSE

19. Poor venipuncture techniques cause hemolysis or tissue thromboplastin

to mix with the blood causing: PROLONGED CT

20. Incubation of plasma at 37c for more than 5 minutes may cause: INC.
FACTOR V AND VIII SHORTENED CLOTTING TIME
WEEK 8 AND 7 11. ADP is used in aggregometry to detect 7. What method uses castor oil=All correct
VWD = FALSE 8. What anticoagulant is used for in-vitro
DASH 2 platelet adhesion test?=CITRATE
12. What is the effect of hemolysis on a
9. What is inhibited by the EDTA?=Thrombin
1. What color of capillary tube be used for hemostasis specimen = A. IN VITRO
capillary tube test? = RED BAND PLATELET AND COAGULATION 10. What happens to the coagulation result if
ACTIVATION OCCUR the specimen is hemolyzed?=Shortened
2. Stasis results in the local accumulation 11. What is inhibited by aspirin?
of coagulation factor VIII and VII = FALSE 13. Aspirin irreversible acetylates the = Cyclooxygenase
(FACTOR VIII and VWF) platelet enzyme cyclooxygenase at the _ at 12. What is the most potent penicicilin that
what position = ACETYL GROUP can affect platelet coagulation?
3. Most specimens for whole-blood

aggregometry are mixed with normal 14. UFH is administered intravenously to =Carbepencillin
saline before testing = 1:1 provide immediate control of coagulation = 13. Patients with erythremia have what

4. Collagen binds GPIa/IIa and GP VI, but it TRUE coagulation result? =Prolonged
induces primary aggregation = FALSE
14. What is the 3rd tube to be filled inETs
15. PRP = 3 CHOICES / ALL ARE
(NO PRIMARY AGGREGATION) order draw?=All correct
CORRECT
15. Associated with loss of carbon dioxide
5. Arachidonic acid is readily oxidized and 16. what is released if the sample is that deteriorates clotting factors if the
must be stored at 20C in the dark = FALSE (- hemolyzed = ADP pH is decresed?=False
20 C) 16. 2-3 standardize puntrure are mode on
the forearm under constant pressure.
6. ADP is the commonly used agonist =TRUE
DASH 3 AND 4
What method is being asked?= IVY’S
7. Primary aggregation involves shape METHOD
change = TRUE 1. The process of blood clotting: 17. Bleeding time is sensitive an dependent
COAGULATION on platelet plug formation=True
8. Platelet aggregometry helps determinethe
2. Platelet Rich Plasma (PRP)= 3 18. Standardize puncture is made on the
cause of thrombocytopenic
choices are correct. earlobe= DUKE’S METHOD
mucocutaneous bleeding = FALSE
(NONTHROMBOCYTOPENIC)
3. What is released if the specimen is 19. The orginal test for platelet function
hemolyzed?=ADP test=BLEEDING TIME TEST
9. If a coagulation specimen collection tube 4. This method allows visualization of 20. Bleeding time was described
is underfilled test result will be prolonged. = platelet morphology=Fornio’s method by=WILLIAM DUKE
TRUE 5. Platelet count depends on its electrical 21. ALL CORRECT
resistance=Automated method 22. False (not frozen
10. Most coagulation testing must be
performed on PPP, which is plasma with a 6. What grading is it if there are multiple
platelet count less than = B. 10,000/UL petechiae over the anterior and back of the
hand=3+
1. The D dimer assay is essential for ruling out venous
thromboembolic disease = TRUE
2. The 2 physiologic human plasminogen = TPA and
UROKINASE
3. Test for Fibrinolysis include = FDPs (Fibrin degradation
products), D-DIMER, PLASMINOGEN, TPA and PAI-1
4. Coagulation factor assay are used to detect and measure
coagulation factor deficiencies = TRUE
5. Partial thromboplastin time is also called APTT = TRUE
6. Heparin may prolong the result of PT = TRUE
7. What should be checked in clotting tests = FIBRIN STRANDS
8. The clot-based coagulation test uses the principle of
modified ACT = TRUE
9. When the fibrinogen level is less than 100 mg/dL APTT will
be prolonged = TRUE
10. Hemorrhage is liekly in vitamin K deficiency and the PTT is
the best indicator = FALSE (PT)
11. Both factor V and Factor VII are reduced in liver disease =
TRUE
12. When the PT is prolonged but the PTT and thrombin clotting
time test results are normal, Factor V may be deficient
= FALSE (FACTOR VII)
13. LAs are members of the antiphospholipid antibody family
and may partially neutrilize PTT reagent phospholipids = FALSE
(PT)
14. Lysed specimen for prothrombin time test = NO EFFECT
15. Factor XIIa forms a complex with two other plasma
components that will initiate invitro coagulation = BOTH
FITZGERALD FACTOR (HMWK) AND FLETECH FACTOR
(PREKALLIKREIN)
16. Lee and White clotting time was used as a screening test
to measure all stage in the extrinsic pathway And used to
monitorcoumadin therapy = BOTH ARE INCORRECT
1. Platelet factor 3 is released when there is platelet
activation thus it will participates in = FORMATION OF
THROMBIN
2. Lee and White clotting time was used as a screening test
to measure all stage in the extrinsic pathway And used to
monitorcoumadin therapy = BOTH ARE INCORRECT
3. All coagulation studies are carried out at what
temperature
= 37 CELSIUS
4. The prothrombin time is useful in the screening
procedurefor the extrinsic to include defects in factor II and
V = TRUE
5. Prothrombin Time = 1 OF THE CHOICES IS CORRECT
6. Incubation of plasma ofthe plasma at 36 for more than 5
mincause = INCREASE FACTOR V; SHORTENED
7. What should be checked in clotting tests = FIBRIN
STRANDS
8. What could be the problem in patients with abnormal
intrinsic and common pathway = LIVER DISEASE
9. PRT is more sensitive method than the coagulation
time ofwhole blood. = TRUE
10. Serial dilutions of plasma are diluted with thrombin =
FIBRINOGEN TITER METHODS
 3.2 % (0.109 M) is the anticoagulant (i.e. _ or is the required storage temperature for
blue top) often used for coagulation testing. PT and aPPT with no unfractionated heparin present in specimen as well
o sodium citrate as PT and aPPT with unfractionated heparin is present in specimen.
o Refrigerator (2-4 C) or Room Temp (18-24 C)
: is the ratio for anticoagulant to blood?
o 1:9 Pre-analytical components of coagulation testing includes:
o specimen labeling, collection, transport, processing,
A short draw of blood prolongs results because.... and storage
o there is an excess of anticoagulant neutralizing the test
reagant calcium Analytical components of coagulation testing includes:
o procedure, QC, maintenance, linearity
In a short draw, when there is a clot in the specimen it becomes determination, reference range determination,
_ competency testing
o useless
Post-analytical components of coagulation testing includes:
In a short draw, when a traumatic venipuncture occurs it can _ o review of patient results (e.g. delta check),
autoverification of results via computer interface,
results
posting of patient results, maintenance of patient
o falsely shorten
records
In a short draw, when there is visible hemolysis it indicates that the results
are
o unreliable

_ is a mechanical instrument where the difference between the

initial absorbance and the absorbance at the maximum slope of the clotting
curve is determined.
o Lipemic

Storage time for PT with no unfractionated heparin present in specimen

o 24 hours

Storage time for aPTT with no unfractionated heparin present in specimen

o 4 hours

Storage time for aPTT for unfractionated heparin therapy

o separate plasma from red cells within 1 hour, test within
4 hours

Storage time for PT when unfractionated heparin is present in specimen

o separate plasma from red cells within 1 hour, test within
4 hours