BVMLT-302 Routine and Special Hematological Test

Unit 1
Multiple Choice Questions-
1.In Sahli’s Method of Hb estimation, blood is converted into which solution.
a. Alkali hematin
b. Acid hematin
c. Oxyhemoglobin
d. Sulphhemoglobin
2.Which haemoglobin is detected by Sahli’s Method.
a) Carboxyhaemoglobin
b) Sulphhemoglobin
c) Methaemoglobin
d) Oxyhemoglobin
3.In Sahli’s method of Hb estimation……………..meniscus is used for reading Hb value in Sahli’s tube.
a. Upper meniscus
b. Middle meniscus
c. Lower meniscus
d. None option
4.In Wu’s method of Hb estimation, haemoglobin is converted into
a) Alkali hematin
b) Acid hematin
c) Cyanmethemoglobin
d) Neutral hematin
5. Which hematin contains colloidal system of hemin and denatured globin?
a) Acid hematin
b) Neutral hematin
c) Cyanmet hematin
d) All options
6. In which method of Hb estimation, haemoglobin is converted into carboxyhemoglobin?
a) Oxyhemoglobin Method
b) Wu’s Method
c) Haldane Gower’s Method
d) Cyanmethemoglobin method
7.In which method of Hb estimation, reduced and oxygenated Hemoglobin is estimated?
a) Sahli’s Method
b) Haldane Gower’s Method
c) Specific gravity Method
d) Cyanmethemoglobin Method
8. One Gram haemoglobin contains……..% iron.
a) 0.17%
b) 0.34%
c) 0.52%
d) 0.72%
9. In Sahli’s hemoglobinometer what is the least count of Hb%0.
a) 0.1gm%
b) 0.2 gm%
c) 0.3 gm%
d) 0.4 gm%
10. …………..from of iron is present in haemoglobin molecule.
a) Ferric form
b) Ferrous form
c) Both 1 & 2
d) None option

Unit 1
One Word Questions-
1. What kind of haemoglobin is detected by sahli’s Method- Oxyhaemoglobin
2. Commonly employed RBC diluting fluid – Hayem’s fluid
3. Total erythrocyte count is decreased in- Anemia
4. During Erythropoiesis the time required for production of stem cell to the erythrocyte is about –
7days
5. Reticulocytes are stained with which type of stain – Brilliant Cresyl Blue.
6. What is the formula of absolute neutrophil count with the help of DLC and TLC -TLCX Neutrophil
%/100
7. What is the normal percentage of Basophil in TLC -0-2%
8. Which is the parent cell of platelet- lymphoblast
9. Average life space of platelet is -7-10 days
10. Packed red cells are given to patients who needs-RBC

Unit 2
1.Hemoglobin is catabolized in reticuloendothelial cells in which part of body.
a) Spleen
b) Bone marrow
c) Liver
d) All options
2.MCV may be spuriously high in the presence of high titre
a) Antigen
b) Antibody
c) Complement
d) Autoantibody
3.The average quantity of haemoglobin present in each cell is known as
a) MCV
b) MCHC
c) MCH
d) Colour index
4. What is the normal range of MCH?
a) 15-23 picogram
b) 8-14 picogram
c) 27-32 picogram
d) 32.36 picogram
5.The amount of haemoglobin present per unit volume of the red cell is called
a) MCH
b) MCHC
c) MCV
d) MCD
6.what is the normal value of MCHC?
a) 12-18%
b) 22-26%
c) 32-38%
d) 41-46%
7.Normal value of MCD is
a) 3.4 micrometre to 4.3 micrometre
b) 5.2 micrometre to 6.4 micrometre
c) 6.9 micrometre to 8.0 micrometre
d) 8.2 micrometre to 9.3 micrometre
8.In which anemia MCV & MCH is higher than normal and MCHC is normal?
a) Microcytic anemia
b) Macrocytic anemia
c) Hemolytic anemia
d) Normocytic anemia
9.In which anemia, MCV & MCH is less than normal & MCHC is normal?
a) Microcytic anemia
b) Macrocytic anemia
c) Thalassemia
d) Hemolytic anemia
10.The mean haemoglobin content of a single cell as compared with that of an arbitrary normal cell.
a) Microcytic anemia
b) Mean cell volume
c) Colour index
d) None option

Unit 2
1. In which pathological condition, PCB is high-Burns.
2. What is the average diameter of reticulocyte- 8Micron
3. DLC is done in Body region of the smear.
4. The widely used fixative to fix thin blood films in Haematology lab is Methanol absolute.
5. Reticulocyte count is increased is Reticulocytotic
6. Normal range of reticulocyte count is -0.2 to 2%
7. The bore diameter of win Trobe tube is about - 3mm
8. Our body’s 70% iron is stored in – haemoglobin
9. Abnormal variation in the size of RBC known as Anisocytosis
10. The specific gravity of Red blood cells – 1.093
 Unit 3

1.In Children, bone marrow biopsies are taken from

a) Proximal end of tibia
b) Ribs
c) Iliac crest
d) Vertebral bodies
2.In adult, bone marrow biopsies are taken from
a) Iliac crest
b) Sternum
c) Cranium
d) Both 1 &2
3.The differential count of the smear of bone marrow is called
a) Myelocyte
b) Hemogram
c) Myelogram
d) Coagulogram
4.Bone marrow activity can be assessed by doing
a) Total count
b) Arneth count
c) Differential count
d) Both 1 & 3
5.In normal bone marrow, how many granulocyte precursor cells are present per 100 nucleated cells.
a) Approximately 10
b) Approximately 20
c) Approximately 40
d) Approximately 50
6.Bone marrow cells which survive and proliferate on culturing are called
a) Progenitor cells
b) Colony-forming cells
c) Both 1 & 2
d) Plasma cells
7.What is the total volume of the bone marrow in adults?
a) 1000 ml
b) 2000 ml
c) 3000ml
d) 4000 ml
8.Bone marrow act as………tissue
a) Epithelial tissue
b) Connective tissue
c) Muscular tissue
d) Nervous tissue
9.Yellow marrow consist which type of cells.
a) Nerve cell
b) Fat cells
c) Plasma cells
d) None option
10.In which condition, bone marrow examination is done?
a) Aplastic anemia
b) Aleukemic leukemia
c) Multiple myeloma
d) All options

Unit 3
1. Heinz body in RBC can be present in chemical poisoning.
2. During granulocytes production in bone marrow myeloblast is convert into Promyelocytes
3. Which anticoagulant is considered best for osmotic fragility test Heparin.
4. What is the normal range of osmotic fragility 0.50% saline
5. Name any alternative test for saliva fragility test Acidified glycolysis test
6. In which blood RBC are more fragile venous blood.
7. Deficiencies of which enzyme causes increased red cell fragility G6PD
8. In which anemia osmotic fragility is increased Elliptocytosis
9. What percent of the total volume of blood is plasma – 55%
10. What is the colons of EDTA contains for blood collection Pink.
 BVMLT-302
Routine Haematological Test
Short Questions
1. Is a reticulocyte count in a CBC? What does the reticulocyte count indicate?
2. What test are done to diagnose parasites? Can stool test detect parasites?
3. What test are done for sickle cell anemia?
4. What is Haematology test?
5. What is the normal range for Haematocrit?
6. Why is screening for sickle cell important?
7. How determine the anti-D antibody titer?
8. What causes high Haematocrit?
9. What is erythrocyte sedimentation rate?
10. Write the steps to prepare blood smear?

Long Questions
1. What do you mean by erythrocyte sedimentation rate and what happens when its rate is high?
2. What are the different methods of Haemoglobin estimation? Describe in detail.
3. What is the difference between Howell Jolly bodies and Heinz bodies? How do you stain Heinz
bodies?
4. How to perform indirect coomb’s test?
5. What is foetal haemoglobin? Give its characteristics?
6. What is special haematological test?
7. What is haemoglobin electrophoresis?
8. Define osmotic fragility test? Give its significance.
9. What is the significance of mean cell volume (MCV)? Give the conditions in which it is high?
10. How bone marrow smear is prepared for microscopic examination? Give all the steps.
BVMLT-302
Long Questions:
Unit-1
1. What is TLC? Explain its procedure, normal range and clinical significance.
2. Explain the sahli’s methods of Hemoglobin estimation? Describe in detail.
3. What are the different methods of hematocrit estimation? Describe in detail.
4. Describe the composition of blood. What is ESR and its normal ranges.
5. Describe the spectrophotometric method of hemoglobin estimation.
6. How to estimate total erythrocyte count with RBCs equipment? Write its interpretation and reference
ranges.
7. What is the manual test for platelet count? Explain in detail.

Unit-2
1. Explain the different red blood indices.
2. How to calculate red blood indices and explain their clinical significance?
3. How to study blood smear? Write about the abnormalities and diagnostic significance?
4. How to count reticulocytes by different methods? Describe in details and Write about its
pathophysiology.
5. How to count DLC? Explain the pathophysiology.
6. Describe the WBC cells with the help of diagram and identifying features.
7. How to prepare a peripheral blood smear? Write the characteristics of an ideal blood smear.

Unit-3
1. What is the difference between Howell Jolly bodies and Heinz bodies? How do you stain Heinz
bodies?
2. What is fetal hemoglobin? Give its characteristics?
3. What is special hematological test?
4. What is hemoglobin electrophoresis?
5. Define osmotic fragility test? Give its significance.
6. Write about fluorescent microscopy in detail and what are its uses?
7. Explain about Babesiosis in detail.
8. Describe about any one in detail:
a) Leishmaniasis
b) Lupus Erythematosus
c) Filariasis