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  • 975.55 S.aureus

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    Alvaro NAVARRO
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    This document provides instructions for using the surface plating method to isolate and enumerate Staphylococcus aureus in foods. It describes using Baird-Parker medium plates and spreading 1 mL of the food sample suspension over triplicate plates using a sterile bent glass rod. The plates are incubated for 45-48 hours at 35-37°C and colonies are counted. Colonies are selected from the appropriate dilution plates and tested for coagulase production. The number of coagulase-positive colonies is multiplied by the dilution factor and reported as S. aureus per gram of food tested.

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    This document provides instructions for using the surface plating method to isolate and enumerate Staphylococcus aureus in foods. It describes using Baird-Parker medium plates and spreading 1 mL of the food sample suspension over triplicate plates using a sterile bent glass rod. The plates are incubated for 45-48 hours at 35-37°C and colonies are counted. Colonies are selected from the appropriate dilution plates and tested for coagulase production. The number of coagulase-positive colonies is multiplied by the dilution factor and reported as S. aureus per gram of food tested.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    701 views1 page

    975.55 S.aureus

    Uploaded by

    Alvaro NAVARRO
    This document provides instructions for using the surface plating method to isolate and enumerate Staphylococcus aureus in foods. It describes using Baird-Parker medium plates and spreading 1 mL of the food sample suspension over triplicate plates using a sterile bent glass rod. The plates are incubated for 45-48 hours at 35-37°C and colonies are counted. Colonies are selected from the appropriate dilution plates and tested for coagulase production. The number of coagulase-positive colonies is multiplied by the dilution factor and reported as S. aureus per gram of food tested.

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    © All Rights Reserved
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    of 1

    17.5.

    02 Retain plates in upright position until inoculum is absorbed by


    AOAC Official Method 975.55 medium (ca 10 min on properly dried plates). If inoculum is not
    Staphylococcus aureus in Foods readily absorbed, plates may be placed in incubator in upright
    Surface Plating Method for Isolation and Enumeration position ca 1 h before inverting. Invert plates and incubate 45–48 h at
    First Action 1975 35°–37°C. Select plates containing 20–200 colonies, unless only
    Final Action 1976 plates at lower dilutions (>200 colonies) have col onies with
    [Applicable for general purpose use in testing foods expected to typical appearance of S. aureus, 987.09E (see 17.5.01). If several
    contain ≥10 cells of S. aureus/g. For small numbers, see 987.09 (see types of colonies are observed which appear to be S. aureus,
    17.5.01).] count num ber of col o nies of each type and re cord counts
    separately. When plates at lowest dilution plated contain <20
    A. Apparatus
    colonies, these may be used. If plates containing >200 colonies
    Sterile, bent glass streaking rods.—Hockey stick or hoe-shape,
    have colonies with typical appearance of S. aureus and typical
    with fire-polished ends, 3–4 mm di am e ter, 15–20 cm long,
    colonies do not appear at higher dilutions, use these plates for
    with an gled spread ing sur face 45–55 mm long.
    enumeration of S. aureus, but do not count nontypical colonies.
    B. Determination Select one colony of each type counted and test for coagulase
    At each dilution plated, aseptically transfer 1 mL test sample production, 987.09F (see 17.5.01). Add number of colonies on
    sus pen sion, 987.09C (see 17.5.01), to trip li cate plates of trip li cate plates rep re sented by col o nies giv ing pos i tive
    Baird-Parker medium, 987.09B(e)(3) (see 17.5.01), and equitably coagulase test and multiply by test sample dilution factor. Report
    distribute the 1 mL inoculum over the triplicate plates (e.g., this number as number of S. aureus/g of food tested.
    0.4–0.3–0.3 mL). Spread inoculum over surface of agar using
    sterile, bent glass streaking rods. Avoid extreme edges of plate. Reference: JAOAC 58, 1154(1975).

     2005 AOAC INTERNATIONAL

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