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  • Practica 6

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    Juan Camilo Duque
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    The document describes a series of calculations to determine the volume of stock solution and water needed for solutions of varying concentration, using an initial stock solution of 1 M concentration. Absorbance measurements are taken of the solutions and a calibration curve is plotted of absorbance versus concentration. The calibration curve equation is used to interpolate concentrations of 136 mM and 419 mM from given absorbance values, with the interpolation expected to have higher precision than extrapolating from the curve.

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    The document describes a series of calculations to determine the volume of stock solution and water needed for solutions of varying concentration, using an initial stock solution of 1 M concentration. Absorbance measurements are taken of the solutions and a calibration curve is plotted of absorbance versus concentration. The calibration curve equation is used to interpolate concentrations of 136 mM and 419 mM from given absorbance values, with the interpolation expected to have higher precision than extrapolating from the curve.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    17 views3 pages

    Practica 6

    Uploaded by

    Juan Camilo Duque
    The document describes a series of calculations to determine the volume of stock solution and water needed for solutions of varying concentration, using an initial stock solution of 1 M concentration. Absorbance measurements are taken of the solutions and a calibration curve is plotted of absorbance versus concentration. The calibration curve equation is used to interpolate concentrations of 136 mM and 419 mM from given absorbance values, with the interpolation expected to have higher precision than extrapolating from the curve.

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    © All Rights Reserved
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    of 3

    C 1∗V 1=C 2∗V 2

    C 2∗V 2
    V 1=
    C1

    C1 = 1.0 M
    V2 = 10.0 mL

    1. C2 = 10 mM = 0.01 M
    C 2∗V 2 0. 01 M∗10 .0 mL
    V 1= = =0.1 mL
    C1 1 .0 M
    VH2O = Vfinal – V1 = 10.0 mL – 0.1 mL = 9.9 mL

    2. C2 = 40 mM = 0.04 M
    C 2∗V 2 0.0 4 M ∗10 .0 mL
    V 1= = =0. 4 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 0.4 mL = 9.6 mL

    3. C2 = 80 mM = 0.08 M
    C 2∗V 2 0.0 8 M∗10 .0 mL
    V 1= = =0. 8 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 0.8 mL = 9.2 mL

    4. C2 = 120 mM = 0.12 M
    C 2∗V 2 0.12 M∗10 .0 mL
    V 1= = =1.2 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 1.2 mL = 8.8 mL

    5. C2 = 160 mM = 0.16 M
    C 2∗V 2 0.16 M ∗10.0 mL
    V 1= = =1.6 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 1.6 mL = 8.4 mL

    6. C2 = 200 mM = 0.20 M
    C 2∗V 2 0.20 M∗10.0 mL
    V 1= = =2.0 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 2.0 mL = 8.0 mL

    7. C2 = 250 mM = 0.25 M
    C 2∗V 2 0.25 M∗10.0 mL
    V 1= = =2.5 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 2.5 mL = 7.5 mL

    8. C2 = 300 mM = 0.30 M
    C 2∗V 2 0.30 M∗10 .0 mL
    V 1= = =3.0 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 3.0 mL = 7.0 mL

    9. C2 = 350 mM = 0.35 M
    C 2∗V 2 0.35 M∗10 .0 mL
    V 1= = =3.5 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 3.5 mL = 6.5 mL

    10. C2 = 400 mM = 0.40 M


    C 2∗V 2 0. 40 M∗10 .0 mL
    V 1= = =4.0 mL
    C1 1.0 M
    VH2O = Vfinal – V1 = 10.0 mL – 4.0 mL = 6.0 mL

    Volumen de
    Concentración Volumen de Volumen final
    Datos solucion stock Absorbancia
    (mM) agua (mL) (mL)
    (mL)
    1 10 0.1 9.9 0.04 10
    2 40 0.4 9.6 0.19 10
    3 80 0.8 9.2 0.38 10
    4 120 1.2 8.8 0.57 10
    5 160 1.6 8.4 0.75 10
    6 200 2.0 8.0 0.95 10
    7 250 2.5 7.5 1.18 10
    8 300 3.0 7.0 1.42 10
    9 350 3.5 6.5 1.65 10
    10 400 4.0 6.0 1.89 10
    Absorbancia VS Concentración
    2
    1.8 f(x) = 0 x − 0
    1.6
    1.4
    Absorbancia

    1.2
    1
    0.8
    0.6
    0.4
    0.2
    0
    0 50 100 150 200 250 300 350 400 450
    Concentración (mM)

    y = 0.0047x - 0.0012
    x = (y + 0.0012) / 0.0047
    x = (0.64 + 0.0012) / 0.0047
    x = 136 mM

    y = 0.0047x - 0.0012
    x = (y + 0.0012) / 0.0047
    x = (1.97 + 0.0012) / 0.0047
    x = 419 mM

    La precisión de los datos obtenidos será mayor en la interpolación de la gráfica (curva de


    calibración) porque estamos calculando valores que están entre el rango de datos, en cambio
    en la extrapolación será menor porque son valores por fuera del rango de datos.

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