Infectious Disease Testing

UriSelect™4 Medium
Direct Identification Visibly Reliable
UriSelect 4 ™

Non selective chromogenic medium for the isolation, differentiation

and enumeration of urinary tract infections pathogens within 18-24 hours:
■ Isolation and identification of both Gram-positive and Gram-negative pathogens in a single plate
■ Chromogenic mix allowing the detection of specific enzymes activities
■ Direct identification of E. coli, Enterococcus spp. and Proteus mirabilis via revelation
and visualization of enzymatic activity of bacteria
■ Presumptive identification of KESC, Streptococci and Staphylococci (diffentiation of S. saprophyticus)
■ Inhibition of Proteus spp. swarming with an original formulation

Assay Procedure

Urine specimen inoculation with a 10 μL calibrated loop

SS S
Incubation 18-24 hrs at 35-37°C

Enumeration:
Compare the density of colonies present on the plates, with those illustrated below:

106 CFU/mL 105 CFU/mL 104 CFU/mL 103 CFU/mL

E. coli
24 hrs

Proteus mirabilis
24 hrs
S

Interpretation of bacteriuria:
Counting of well-isolated colonies on UriSelect™ 4 should be possible up to 104 CFU/mL
of urine. Above that concentration, presence of confluent colonies does not result in accurate counts.
Direct Identification of E. coli, Proteus mirabilis
and Enterococcus spp.

Escherichia coli Enterococcus spp.

Pink colonies: Turquoise-blue colonies:

β-Galactosidase β-Glucosidase
Indole

Proteus mirabilis

Orange-brown colonies with

possible brownish halo:
TDA , Indole

Presumptive Identification of KESC

Klebsiella Serratia
pneumoniae marcescens

Enterobacter Citrobacter
cloacae koseri
Examples of Other Pathogens Growth

Staphylococcus Staphylococcus
saprophyticus aureus

Staphylococcus Streptococcus
epidermidis agalactiae

Pseudomonas Candida
aeruginosa albicans

Acinetobacter
baumanii
Pathogens Identification
Observation of the color of colonies:

S
Pink colonies Turquoise blue Orange-brown colonies Blue-purple colonies White, other color
= β-Galactosidase ⊕ colonies with a possible = β-Galactosidase ⊕ or colorless colonies
= β-Glucosidase ⊕ brownish halo and
= TDA **⊕ β-Glucosidase ⊕
S

S
S

Test for indole Examine under Test for indole Examine under
directly on a colony microscope directly on a colony microscope
S

S
S
S

⊕ ⊝ Gram ⊕ Cocci ⊝ ⊕ Gram ⊝ Bacilli

E.coli Enterococci Proteus Proteus spp., very likely
mirabilis Morganella spp., KESC *
Providencia spp.
S

S
S

*K lebsiella, Enterobacter,

Serratia and Citrobacter
** Tryptophan Desaminase Complete the identification using a conventional procedure

Visualization and Identification of Mixed Cultures

Contrasting colors of colonies enhanced by a pearl-grey opaque background allow for an optimal visualization
of polymicrobial mixtures.

18 hrs mixed culture of 18 hrs mixed culture of E. coli,

Klebsiella spp. and Enterococcus spp. and
Enterococcus spp. Proteus spp.
Ordering Information
Catalog # Description Catalog # Description
Urinary Tract Infections Rapid Detection of Resistance to Third Generation Cephalosporins
63726 UriSelect™4, 20 plates x 90 mm for Enterobacteriaceae
63727 UriSelect™4, 100 plates x 90 mm 68250 b LACTA™ Test, 50 tests
64694 UriSelect™4 (dehydrated), 500 g Rapid Detection of Carbapenemase-Producing Enterobacteriaceae
55313 Kovacs reagent, 2 vials x 15 ml 68260 b CARBA Test, 25 tests
63713 Urea Indole medium, 50 ampules x 1 ml Rapid Identification of Carbapenemases Types
53834 O.X. (Oxidase), 2 x 50 disks K-15R1 OXA-48 K-SeT*
56350 Novobiocin (5 µg), 30 disks K-15R2 KPC K-SeT*
56356 Pastorex™ Staph Plus, 50 tests
56353 Pastorex™ Staph Plus, 5 x 50 tests * Manufactured by and distributed by Bio-Rad.
61721 Pastorex™ Strep, 60 tests Ask for availability in your country.
56513 AuxaColor™ 2 kit, 20 tests

Visit www.bio-rad.com/select-chromogenicmedia Other Chromogenic Media

63757 MRSASelect™II, 20 plates x 90 mm
for more information.
63748 SASelect™, 20 plates x 90 mm
63751 VRESelect™, 20 plates x 90 mm
63750 StrepBSelect™, 20 plates x 90 mm
63740 CandiSelect™, 20 plates x 90 mm

SASelect ™

VRESelect ™

MRSASelect™ II

UriSelect ™4

StrepBSelect ™

CandiSelect ™ 

Chromogenic Media Range

Bio-Rad
Laboratories

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