Professional Documents
Culture Documents
BS Medical Technology
SY. 2021-2022
MT111
(Principles of Medical Laboratory Science Practice 1
(Intro to Med Lab Science, Lab Safety and Waste
Management))
BSMT 1
Learning Module
Midterm Term, First Semester, AY 2021 - 2022
MT111- Principles of Medical Laboratory Science Practice 1 (Intro to Med Lab Science, Lab Safety
and Waste Management
CLASS CODE:dogze2b
Brief Introduction:
The Midterm Term for this course will cover One unit consist of Three Modules
Learning Outcomes:
At the end of this term, the students are expected to:
1. Comprehend basic ideas about contemporary science and technology;
2. Describe the tools and equipment, products and processes commonly encountered by a Medical Technologist.
3. Differentiate equipment, products and processes commonly encountered by a Medical Technologist.
1. Online quiz- refer to the schedule that will be posted on our Group chat/Google Classroom
2. Online exam- refer to the schedule that will be posted on our Group chat/Google Classroom
3. Activities- Modular Activities are attached at the end of each Unit.
4. Submit your activities during the distribution of Prefinal Modules.
GRADING SYSTEM:
PERCENTAGE DISTRIBUTION FOR EACH TERM:
40% for Major Exams
20% PRELIM
60% for Performance Task/Outcomes
30% Modular Activities 25% MIDTERM
15% Summative Quiz 25% PREFINAL
15% Project Requirement 30% FINAL
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INTRODUCTION:
Science (from the Latin word scientia or "knowledge") is regarded as the heart of technology. It is one of the main factors
behind the invention and development of many products, such as computers and cellphones, which people currently use.
As an academic field, it has never stopped inspiring and nurturing minds to think, discover, and investigate new ideas.
The concept of science can be summarized by the acronym using the letters of the word itself (Table 1.1).
Science is……
S Systematic an organized body of knowledge derived from a sound
methodology. It can be clearly explained and consistently applied.
C Conceptual abstract and logical.
I Integrative interdisciplinary; it offers a holistic approach that includes or uses
both classical and modern concepts.
E Evolving constantly changing. Scientific concepts always become better
and more advanced or complex
N Novel geared towards inventing products that are new and original.
C Contemporary up-to-date.
E Exploratory a never-ending process of discovery and fact finding.
Technology (from the Greek word tekhnologia or “systematic treatment') refers to the practical application of an
organized body of knowledge. Based on the results of scientific research, it is intended to provide benefits to people.
Some regard it as an "applied science" because it utilizes scientific concepts to produce or create something which may
be in the form of goods, equipment, gadgets or even techniques.
Technology is classified into three broad categories: tools, products, and processes.
1. CENTRIFUGE
2. OVEN
An oven is a thermostatically which is used for dry heating. Ovens in the laboratory utilize the
principle of high-forced volume thermal convection.
3. AUTOCLAVE
An autoclave is a device used to sterilize equipment and culture media by subjecting them
to high-pressure saturated steam at 121 °C for 15-20 minutes. The word autoclave comes
from the Greek word auto, meaning "self," and the Latin word clavis, meaning "key". An
autoclave literally means a self-locking device.
4 | MIDTERM- MT111
4. MICROTOME
A microtome is a machine
used to cut uniformly thin }|
slices of tissue blocks,
known as sections.
Microtomes use various
types of knives (e.g., steel, 10. PIPETTES
alloy steel, glass, or Pipettes are also called pipets,
diamond), depending on pipettors, or chemical
the kind and thickness of droppers. They are tools used to measure and transfer
the specimen to be sliced. small volumes of liquids. Pipettes are designed to have
5. WATER BATH differing levels of accuracy and precision. A simple
glasspiece pipette, for instance, is less complex than an
adjustable orelectronic one. To manipulate a pipette, a
A water bath is a water-filled partial vacuum above the liquid-holding chamber must
vessel used in laboratories to be created and carefully released to draw up and
incubate samples dispense the liquid.
maintained at a constant
temperature. 11. INCUBATOR
An incubator is an apparatus
6. SPHYGMOMANOMETER used to grow and maintain
microbiological and cell
A sphygmomanometer is a cultures. The environmental
device used to measure blood
pressure. It consists of a
mercury or mechanical conditions inside it such as
manometer to indicate the temperature, humidity, and
pressure, and a cuff which is carbon dioxide and oxygen
inflatable to temporarily levels can be controlled.
restrict the blood flow.
7. SPECTROPHOTOMETER
A spectrophotometer is
also called a
photometer. It
quantitatively measures
the amount of light that
a sample absorbs.
8. COUNTING CHAMBER
A counting chamber is a
thick specimen slide
engraved with fine-quality,
aser-etched grids. It is used
in counting the cellular
elements of the blood such
as red blood cells, white
blood cells, and platelets.
A WBC differential
counter is a handy
device used in
hematology to manually
count and classify white
blood cells. Sounds are
generated each time its
keypad is pressed. The keypad is locked once 100 cells
have been counted.
5 | MIDTERM- MT111
is an elastic bandage
applied to the patient's
extremities to constrict
blood vessels and
make the venipuncture
site more prominent
during phlebotomy.
PROCESSES
Different routine tests or processes are performed in clinical laboratories by registered medical technologists. Usually,
these tests or processes are requested by physicians to aid and support their clinical diagnosis.
1. STOOL EXAMINATION
- Involves the collection and analysis of fecal specimens to identify the presence or absence of certain medical
conditions.
B. MICROBIOLOGICAL EXAMINATION
-aim to find out if bacteria, viruses, and parasites
are present in the stool sample
2. ROUTINE URINALYSIS
- is a laboratory test that includes the physical,
chemical, and microscopic examination of a urine
sample
1. Specific gravity
Urine specific gravity is a measure of urine concentration. This test simply indicates how concentrated the urine is.
Specific gravity measurements are a comparison of the amount of substances dissolved in urine as compared to pure
water. If there were no substances present, the specific gravity of the urine would be 1.000 (the same as pure water).
Since all urine has some substances in it, a urine SG of 1.000 is not possible. If a person drinks excessive quantities of
water in a short period of time or gets an intravenous (IV) infusion of large volumes of fluid, then the urine specific
gravity may be very close to that of water. The upper limit of the test pad, a specific gravity of 1.035, indicates
concentrated urine, one with many substances in a limited amount of water.
7 | MIDTERM- MT111
2. pH
As with specific gravity, there are typical but not "abnormal" urine pH values. The urine is usually slightly acidic, about
pH 6, but can range from 4.5-8. The kidneys play an important role in maintaining the acid-base balance of the body.
Therefore, any condition that produces acids or bases in the body, such as acidosis or alkalosis, or the ingestion of
acidic or basic foods can directly affect urine pH.
Some of the substances dissolved in urine will precipitate out to form crystals when the urine is acidic; others will form
crystals when the urine is basic. If crystals form while the urine is being produced in the kidneys, a kidney stone or
"calculus" can develop. By modifying urine pH through diet or medications, the formation of these crystals can be
reduced or eliminated.
3. Bilirubin
This test screens for bilirubin in the urine. Bilirubin is not present in the urine of normal, healthy individuals. It is a waste
product that is produced by the liver from the hemoglobin of RBCs that are broken down and removed from circulation.
It becomes a component of bile, a fluid that is released into the intestines to aid in food digestion.
In certain liver diseases, such as biliary obstruction or hepatitis, excess bilirubin can build up in the blood and is
eliminated in urine. The presence of bilirubin in urine is an early indicator of liver disease and can occur before clinical
symptoms such as jaundice develop.
The results of this test will be considered along with the result of urobilinogen (below). If positive, the healthcare
practitioner will likely follow up with other laboratory tests, such as a liver panel, to help establish a diagnosis.
4. Urobilinogen
This test screens for urobilinogen in the urine. The results are considered along with those for urine bilirubin (above).
Urobilinogen is normally present in urine in low concentrations. It is formed in the intestine from bilirubin, and a portion
of it is absorbed back into the blood. Positive test results may indicate liver diseases such as viral hepatitis, cirrhosis,
liver damage due to drugs or toxic substances, or conditions associated with increased RBC destruction (hemolytic
anemia). When urine urobilinogen is low or absent in a person with urine bilirubin and/or signs of liver dysfunction, it
can indicate the presence of hepatic or biliary obstruction.
5. Protein
The protein test pad provides a rough estimate of the amount of albumin in the urine. Albumin makes up about 60% of
the total protein in the blood. Normally, there will be no protein or a small amount of protein in the urine. When urine
protein is elevated, a person has a condition called proteinuria.
Proteinuria may occasionally be seen in healthy individuals. Healthy people can have temporary or persistent
proteinuria due to stress, exercise, fever, aspirin therapy, or exposure to cold, for example. Repeat testing may be done
once these conditions have resolved to determine whether the proteinuria is persistent.
If trace amounts of protein are detected, and depending on the person's signs, symptoms and medical history, a repeat
urinalysis and dipstick protein may be performed at a later time to see if there is still protein in the urine or if it has
dropped back to undetectable levels.
If a large amount of protein is detected on a urinalysis and/or if the protein persists in repeated tests, a 24-hour urine
protein test may be used as a follow-up test. Since the dipstick primarily measures albumin, the 24-hour urine protein
test also may be ordered if a healthcare practitioner suspects that proteins other than albumin are being released into
the urine.
Protein in the urine may be a sign of kidney disease. Small amounts of albumin may be found in the urine when kidney
dysfunction begins to develop. A different test called a urine albumin test detects and measures small amounts of
albumin in the urine. The urine albumin test is more sensitive than a dipstick urinalysis and is routinely used to screen
people with chronic conditions that put them at risk for kidney disease, such as diabetes and high blood pressure. (See
the article on Urine Albumin.)
Proteinuria may also be associated with many other diseases and conditions. A healthcare practitioner may order other
types of follow-up tests to help determine the cause of protein in the urine.
6. Glucose
Glucose is normally not present in urine. When glucose is present, the condition is called glucosuria.
It results from either:
1. An excessively high glucose level in the blood, such as may be seen with people who have uncontrolled diabetes
2. A reduction in the "renal threshold;" when blood glucose levels reach a certain concentration, the kidneys begin to
eliminate glucose into the urine to decrease blood concentrations. Sometimes the threshold concentration is reduced
and glucose enters the urine sooner, at a lower blood glucose concentration.
Some other conditions that can cause glucosuria include hormonal disorders, liver disease, medications, and
pregnancy. When glucosuria occurs, other tests such as a fasting blood glucose are usually performed to further
identify the specific cause.
7. Ketones
Ketones are not normally found in the urine. They are intermediate products of fat metabolism. They are produced
when glucose is not available to the body's cells as an energy source. They can form when a person does not eat
enough carbohydrates (for example, in cases of fasting, starvation, or high-protein diets) or when a person's body
cannot use carbohydrates properly. When carbohydrates are not available, the body metabolizes fat instead to get the
energy it needs to keep functioning. Strenuous exercise, exposure to cold, frequent, prolonged vomiting, and several
digestive system diseases can also increase fat metabolism, resulting in ketonuria.
In a person who has diabetes, ketones in urine may also be an early indication of insufficient insulin. With insufficient
insulin, a diabetic cannot process glucose and instead metabolizes fat. This can cause ketones to build up in the blood,
resulting first in ketosis and then progressing to ketoacidosis, a form of metabolic acidosis. Excess ketones and glucose
are dumped into the urine by the kidneys in an effort to flush them from the body. This condition, called diabetic
ketoacidosis (DKA), is most frequently seen with uncontrolled type 1 diabetes and can be a medical emergency.
This test is used to detect hemoglobin in the urine (hemoglobinuria). Hemoglobin is an oxygen-transporting protein
found inside red blood cells (RBCs). Its presence in the urine indicates blood in the urine (known as hematuria).
A small number of RBCs are normally present in urine and usually result in a "negative" chemical test. An increased
amount of hemoglobin and/or increased number of RBCs are detected as a "positive" chemical test result. Results of
this test are typically interpreted along with those from the microscopic examination of the urine to determine whether
RBCs are present in the urine. A positive result on this test with no RBCs present may indicate the presence of
hemoglobin in the urine (which can occur when RBCs have broken apart) or myoglobin from muscle injury. (See the
article on Myoglobin.)
Blood in the urine is not a normal finding, but it is not uncommon and not necessarily a cause for alarm. Your
healthcare practitioner will investigate further to try to determine the source and underlying cause of the blood and may
request repeat testing to determine whether the blood is persistent.
9. Leukocyte esterase
Leukocyte esterase is an enzyme present in most white blood cells (WBCs). A few white blood cells are normally
present in urine and usually give a negative chemical test result. When the number of WBCs in urine increases
significantly, this screening test will become positive. Results of this test will be considered along with a microscopic
examination for WBCs in the urine.
When this test is positive and/or the WBC count in urine is high, it may indicate that there is inflammation in the urinary
tract or kidneys. The most common cause for WBCs in urine (leukocyturia) is a bacterial urinary tract infection (UTI),
such as a bladder or kidney infection. In addition to WBCs, bacteria and RBCs may also be seen in the microscopic
examination. If bacteria are present, the chemical test for nitrite may also be positive.
10. Nitrite
This test detects nitrite and is based upon the fact that many bacteria can convert nitrate (a normal substance in urine)
to nitrite. Normally, the urinary tract and urine are free of bacteria and nitrite. When bacteria enter the urinary tract, they
can cause a urinary tract infection. A positive nitrite test result can indicate a UTI. However, since not all bacteria are
capable of converting nitrate to nitrite, someone can still have a UTI despite a negative nitrite test. The results of this
test will be considered along with the leukocyte esterase (above) and a microscopic examination.
In MICROSCOPIC ANALYSIS, entails the preparation of urine sediments by means of centrifugation. It includes
examination of: cells, casts, crystals, parasites, and other artifacts
Once the identity of the microorganism has been establishes, sensitivity testing follows.
A SENSITIVITY TEST is done to verify what kind of medicine, such aas an antibiotic, will
be effective in combatting the identified causative agent.
5. BLOOD TYPING
9 | MIDTERM- MT111
- is done in the Blood Banking section of the laboratory, also referred to as Blood grouping.
-the primary goal of this laboratory test is to classify a blood sample according to the presence or absence of antigens
inherent in red blood cells. Blood sample obtained from a patient is mixed with a commercially available antiserum.
-AGGLUTINATION REACTION is interpreted as a positive result.
- are panel tests that check the condition and functionality of the different
boDy organs. These tests can be requested by a physician to evaluate a
person's electrolyte balance.
-often require a serum sample. The serum sample is used to elicit a reaction
from the chemical substances present in the reagents. The chemical reaction leads to color changes in the reagents to
be measured by means of a spectrophotometer. The concentration of the substance is directly proportional to the
intensity of light generated.
High-Density
Lipoprotein (HDL)
Normal Range: 60
mg/dL or greater
Creatinine (Creat) High creatinine levels almost always are due 0.6-1.5 mg/dL
to renal damage.
Fasting Blood Sugar (FBS) Fasting blood sugar is measured to assess 70-110 mg/dL
glucose metabolism.
2-hour post-prandial blood sugar (2- Used to assess glucose metabolism. Less than 140 mg/dL
hr PPBS)
Glucose Tolerance Test (GTT) Use to assess glucose metabolism. 30 min: 150-160 mg/dL
1 hour: 160-170 mg/dL
2 hour: 120 mg/dL
3 hour: 70-110 mg/dL
STEPS:
8. GRAM STAINING
-is the most important staining technique in differentiating Gram-positive bacteria
from Gram-negative ones.
-Developed by a Danish microbiologist named Hans Christian Gram
STEPS:
1. SKIN PUNCTURE-
“pricking” or 2. VENIPUNCTURE
“capillary/dermal - is the process of withdrawing venous blood sample
puncture” from the veins
- frequently performed when the patient is a baby or - site of choice is the median cubital vein of
when only a small amount of blood is needed for the antecubital fossa
laboratory examination. - makes duplicate sample
-collected through a skin puncture in the finger, toe, testing
heel, or ear lobe
11 | MIDTERM- MT111
3. ARTERIAL PUNCTURE
- perfomed when blood gas analysis is needed to investigate acid-base balance
- blood sample is directly obtained from the radial, brachial,
femoral artery.
Draw and color 3 products that are not listed in this module. Do it in a separate sheet of bond paper. Pass this modular
activity in hard copy.