FACULTY OF MEDICINE, BIOSCIENCE & NURSING

SCHOOL OF BIOSCIENCE

PRACTICAL REPORT 1a
BMC 112 Laboratory Science & Instrumentation 1

Name: AISSATA DIAWARA

Student ID: BBSH 22106335

Course: Bachelor In

Biomedical Science

Practical Title: Fundamentals of laboratory safety practices and safety equipment

Module Leader: Wong

1. INTRODUCTION
Workers in laboratories are constantly exposed to a variety of dangers, such as radioactive, chemical,
and biological dangers. Safety measures are crucial in labs where potentially hazardous situations
prevail. Safety equipment is used to safeguard lab users from harm or to help them respond to
emergencies. Rules are in place to reduce each individual's risk. Improper handling of chemicals and
lab equipment can cause serious harm or even death. Fires, poisonous gases, needle sticks, chemical
spills, and toxic fumes can all be harmful to laboratory personnel as well as everyone else in or around
the facility.
In this chapter, the learner will study about the rules governing laboratory safety. Included in
laboratory security is:
• Safety protocols for laboratories
• Safety precaution
• Emergency action
• Chemical method
Objective 2.
to guarantee that each student is prepared before entering the lab at all times. Give students
information on laboratory safety as well. Additionally, until the experiment is over, students can be
cured.
3. METHOD
Safety protocols for laboratories:
1. Notifying the teacher of any spills or broken glasses and asking for cleanup instructions.
2. Handwashing before and after entering laboratories and whenever contamination is conceivable.
3. Using the right disinfectant and a sufficient contact time, disinfect lab benches and equipment both
before and after each lab session.
4. Different waste types should be identified and properly disposed of.
5. Completing a laboratory safety agreement and signing it to confirm that the student has read and
comprehends the regulations of the lab.6.Good lab practice, including returning materials to proper
locations, proper care
and handling of equipment, and keeping the bench top clear of extraneous materials.
6. Good laboratory practices, such as putting things back where they belong, handling and caring for
equipment correctly, and keeping the work surface free of clutter.

• Protective procedures:
1. Wearing long pants or dresses (NO shorts, NO sandals).
2. Tying long hair back, wearing personal protective equipment (eye protection,
coats, gloves; glasses may be preferred to contact lenses), and using such
equipment in appropriate situations.
3. Always using appropriate pipetting devices and understanding that mouth pipetting
is forbidden.
4. Never eating or drinking in the laboratory.

• Emergency procedures:
1. Locating and properly using emergency equipment (eye wash stations, first aid
kits, fire extinguishers, chemical safety showers, telephones and emergency
numbers).
2. Reporting all injuries immediately to the instructor.
3. Following proper steps in the event of an emergency.

• Chemical safety:
1. Handle each chemical cautiously as though it were dangerous.
2. Never let a solvent "smell"! To find out what's inside the solvent container, read the label.
3. Ensure that all chemicals are clearly labelled with the name of the substance, the concentration, the
date, and the name of the person in charge.
4. Never refill reagent bottles with chemicals. (Aim for the right quantity and distribute any surplus.)
5. Close the chemical container right away after usage.
6. Adhere to fire standards about storage quantities, permissible cabinet and container types,
appropriate labelling, etc. If unsure about the rules, speak with the building coordinator.
7. Only use combustible and volatile substances in a fume hood. Aerosol-producing procedures should
be carried out in a hood to prevent hazardous substance inhalation.
8. You should never let a solvent touch your skin. gloves are a must.
9. Place trash and broken glass in the correct containers for disposal.
10. Immediately wipe up spills.

4. RESULT
ACTIVITY
List the Protective Procedure in your lab with pictures as proof.

NAME IMAGE DESCRIPTION

Lab coat A lab coat is often a knee-
length, white coat with deep
pockets that is worn in a
medical office or laboratory.
Safety shoes Both formal and casual
safety shoe styles are
available. However, for their
safety, employees need to
wear dependable and sturdy
work shoes.
Glove Every time you come in
contact with blood, mucous
membranes, etc., you must
put on gloves to protect your
hands from germs that could
make you ill.
Mask Students can shield
themselves from the effects
of these chemicals in the lab
by wearing a mask. In the
lab, wearing a mask is
crucial for this reason.
Emergency shower and eye On-site decontamination is
wash provided through emergency
showers and eyewash
stations. They enable
employees to flush harmful
substances that could harm
them away.

Despite robust technical

controls and safety
measures, chemical
exposures can sometimes
happen accidentally.

Tube test A test tube, culture tube, or

sample tube is a transparent,
cylindrical container with an
open end and a closed end
that is generally used for
scientific purposes.
overshoes a shoe worn over a regular
shoe, usually composed of
rubber to safeguard the
regular shoe or of felt to
safeguard a floor surface.
Fire extinguisher movable apparatus that
releases a water jet, foam,
gas, or another substance to
put out a fire.
Binocular microscope a pair of binoculars one that
has two eyepieces, allowing
use of both eyes at once. a
compound microscope has
two lens systems that work
together to magnify the
image created by the system
closest to the object.

Copper sulphate solution Liquid solution of copper

sulphate that range in
concentration from mild to
extremely concentrated. For
applications needing
materials that have been
soliloquized, they are a great
supply of copper sulphate.
specto

Hot air oven Electrically powered hot air

ovens sanitise by applying
dry heat. Pasteur was the
person who first created
them. The temperature is
often managed with a
thermostat.
Weighing boat used for sample weighting;
frequently disposable.

When a precise smaller

volume of a sample solution
Volumetric pipette or reagent needs to be taken,
volumetric pipettes (transfer
or belly pipettes) are
employed in volumetric
analysis.
balance weight distribution that
allows something or
someone to stand

Hotplate magnetic stirrer The addition of a magnetic

within a hot plate makes it a
magnetic stirrer.
Imaging systeme Imaging systems are tools
for measuring the mass,
momentum, energy, or
position of charged particles.
Pipette bulb Using pipette bulbs, often
referred as the pipe bulbs,
pi-petting balls, or pipette
bulbs, you can manually fill
volumetric pipettes, transfer
pipettes, and both.
Glass pepitte a compact device that
usually consists of a thin
tube into which liquid is
sucked by suction.
Titronic burette a piston burette that is best
for manual titrations, precise
dosage applications, and
solution preparation.

Dry block thermostat is an old-fashioned Biosan's

dry block thermostat used
for research. The
thermostat's purpose is to
maintain a constant
temperature for samples in
tubes.

graduated glass pipette 1ml \ glass graduated pipettes

Liquids are dispensed using
pipettes with scales. They
are "Ex" calibrated (to
deliver). The given liquid
volume is referred to as the
imprinted volume.
micro-pipette incredibly tiny liquid
measurements, transfers, or
injections require an
extremely delicate pipette.
Normal bin Sharps and medical waste
Biohazard bin can be disposed of safely
Sharp bin using the sharps container
and medical waste container.

tisus is used to clean hands,

tables, microscopes,

Plastic test tube rack When several solutions are

Wooden test tube rack
now being worked on or
collected simultaneously,
they are very helpful for
organising test tubes.
They typically have six or
twelve wells, respectively,
and can accommodate six or
twelve test tubes. They are
common in many
laboratories due to their
modest volume and small
amount of space occupied.

Biological safety cabinet is a controlled biosafety

level laboratory environment
that is enclosed and
ventilated for dealing with
materials that are (or could
be) contaminated with
pathogens.
centrifuge machine that uses
centrifugal force to
distinguish fluids of
different densities by rapidly
rotating its container's
contents
Incubator enclosed device that houses
preterm babies or infants
that are extremely small and
offers a safe haven for their
care.an apparatus used to
hatch eggs or grow
microorganisms under
controlled conditions.
•
 Vortex mixture mass of fluid or air that is
whirling, notably a
whirlpool or whirwind:
Cell counter offers a quick method to
carry out cell differentials on
a bone marrow aspirate or
peripheral blood sample
Haematology analyzers Complete blood counts
(CBCs), frequently the first
test sought by doctors to
assess a patient's general
health state, are performed
using haematology
analyzers. Red blood cell
(RBC), white blood cell
(WBC), haemoglobin, and
platelet counts are all
included in a complete blood
count, along with hematocrit
values.

5. CONCLUSION
Students learn more about the safety requirements that must be met by all students from
Experiment 1. There are four main safeguards, including chemical safety, protective
safeguards, emergency safeguards, and safeguards for laboratories. Students should be able to
comprehend and follow all regulations in order to ensure that their safety is always put first.
Students learned about accountability and the necessary course of action when anything
negative occurs in the laboratory while learning about laboratory safety procedures. We also
learned the importance of cleanliness in defending our bodies against potential health risks.
Students then learned about all the restrictions before entering the lab as part of preventive
measures. For instance, a student should put on gloves, goggles, a lab coat, and so forth.. As a
result, the student also learnt about the guidelines for conducting the experiment

progress. For instance, instructors counsel students to use the proper equipment for every
experiment and to avoid drinking or eating in the lab.
Students also learned about emergency protocols, including where to find emergency
equipment and how it works, and the right actions to take in case of an emergency. We should
also be aware of each laboratory's instructor and emergency phone number.
Last but not least, students learned about the purposes, dangers, and warnings associated with
each chemical used in the lab as well as how to prevent them. The proper usage of chemicals
and the steps that students should take to stay safe in laboratories were also taught to the
pupils.
6. REFERENCE
George H, Wahl, Jr and Maria T. Gallardo-Williams. (2011). Laboratory Safety
Rules and Regulations.
Anne Marie Helmenstine. (June 26,2018). The 10 Most Important Lb Safety
Rules. Retrieved from https://www.thoughtco.com/important-lab-safety-rules-608156
Stony Brook. (2008). Lab Safety Guide. New York. Retrieved
from https://ehs.stonybrook.edu/programs/laboratory-safety/lab-safety-guide