FACULTY OF MEDICINE, BIOSCIENCE & NURSING

SCHOOL OF BIOSCIENCE

PRACTICAL REPORT 1a
BMC 112 Laboratory Science & Instrumentation 1

Name: AISSATA DIAWARA

Student ID: BBSH 22106335

Course: Bachelor In

Biomedical Science

Practical Title: Fundamentals of laboratory safety practices and safety equipment

Module Leader: Dr. Rafidah

1. INTRODUCTION
In laboratory, workers are always expose to a lot of hazards including, chemicals, biochemicals,
physicals and radioactive hazards. In laboratories where the dangerous conditions might exist, safety
precautions are important. Rules exist to minimize the individual’s risk, and safety equipment is used
to protect the lab user from injury or to assist in responding to an emergency. Lab equipment and
chemicals that are improperly handled can result in personal injury and even death. Chemical spills,
toxic fumes, needle pricks, and fires can harm lab workers and all the people either in or surrounding
of the laboratory.
In this chapter, student will learn about safety rules in laboratory. Laboratory safety
are included:

 Laboratory safety procedure

 Protective procedure
 Emergency procedure
 Chemical procedure
2. OBJECTIVE
To make sure that all students are always prepared before entering the lab. Also give
students knowledge and detail about laboratory safety. Students also can be cured
until the experiment ends.
3. METHOD

 Laboratory safety procedures:

1. Reporting all spills and broken glassware to the instructor and receiving
instructions for clean-up.
2. Washing hands prior to and following laboratories and at any time contamination is
suspected.
3. Disinfecting lab benches and equipment prior to and at the conclusion of each lab
session, using an appropriate disinfectant, and allowing a suitable contact time.
4. Identification and proper disposal of different types of waste.
5. Reading and signing a laboratory safety arrangement indicating that the student
has read and understands the safety rules of the laboratory.
6.Good lab practice, including returning materials to proper locations, proper care
and handling of equipment, and keeping the bench top clear of extraneous materials.

 Protective procedures:
1. Wearing long pants or dresses (NO shorts, NO sandals).
2. Tying long hair back, wearing personal protective equipment (eye protection,
coats, gloves; glasses may be preferred to contact lenses), and using such
equipment in appropriate situations.
3. Always using appropriate pipetting devices and understanding that mouth pipetting
is forbidden.
4. Never eating or drinking in the laboratory.

 Emergency procedures:
1. Locating and properly using emergency equipment (eye wash stations, first aid
kits, fire extinguishers, chemical safety showers, telephones and emergency
numbers).
2. Reporting all injuries immediately to the instructor.
3. Following proper steps in the event of an emergency.

 Chemical safety:
1. Treat every chemical as if it were hazardous.
2. Never "smell" a solvent!! Read the label on the solvent bottle to identify its
contents.
3. Make sure all chemicals are clearly and currently labelled with the substance
name, concentration, date, and name of the individual responsible.
4. Never return chemicals to reagent bottles. (Try for the correct amount and share
any excess).
5. Close chemical bottle immediately after use.
6. Comply with fire regulations concerning storage quantities, types of approved
containers and cabinets, proper labelling, etc. If uncertain about regulations, contact
the building coordinator.
7. Use volatile and flammable compounds only in a fume hood. Procedures that
produce aerosols should be performed in a hood to prevent inhalation of hazardous
material.
8. Never allow a solvent to come in contact with your skin. Always use gloves.
9. Dispose of waste and broken glassware in proper containers.
10. Clean up spills immediately.
4. RESULT
ACTIVITY
List the Protective Procedure in your lab with pictures as proof.

NAME IMAGE DESCRIPTION

Lab coat Lab coat is usually
white,knee-length coat with
deep pocket that is typically
worn in a laboratory or
medecine office
Safety shoes Safety shoes come in many
styles both formal or
informal. However, workers
require reliable and durable
work shoes for their safety
glove Gloves help keep your hands
clean and lessen your chance
of getting germs that can
make you sick we have to
wear gloves every time you
touch blood mucous
membrane etc
mask  By using the mask students
can protect themselves in the
laboratory from the effect of
these gases. That is why
using mask is very important
in the laboratory.
Emergency shower and eye Emergency showers and
wash  eyewash stations provide on-
the-spot decontamination.
They allow workers to flush
away hazardous substances
that can cause injury.
Accidental chemical
exposures can still occur
even with good engineering
controls and safety
precautions

Tube test A test tube, culture tube, or

sample tube is a clear,
cylindrical container that is
typically open at one end
and closed at the other.
overshoes a shoe worn over a normal
shoe, typically made either
of rubber to protect the
normal shoe or of felt to
protect a floor surface.
Fire extinguisher  portable device that
discharges a jet of water,
foam, gas, or other material
to extinguish a fire.
Binocular microscope binocular microscope one
with two eyepieces,
permitting use of both eyes
simultaneously. compound
microscope one consisting of
two lens systems whereby
the image formed by the
system near the object is
magnified by the one nearer
the eye.
Copper sulphate solution Copper sulfate Solutions
are moderate to highly
concentrated liquid
solutions of Copper
Sulfate. They are an
excellent source of Copper
Sulfate for applications
requiring soliloquized
materials
specto

Hot air oven Hot air ovens are electrical

devices which use dry heat
to sterilize. They were
originally developed by
Pasteur. Generally, they use
a thermostat to control the
temperature.
Weighing boat  often disposable, used for
weighing samples.

Volumetric pipettes (transfer

or belly pipette) are used in
Volumetric pipette volumetric analysis, when
there is a need for taking
exact smaller volume of a
sample solution or reagent.
balance  distribution of weight
enabling someone or
something to remain upright
and steady:
Hotplate magnetic stirrer  A hot plate magnetic stirrer
is a hot plate with an added
bonus, it has a magnetic
inside of it.
Imaging systeme Imaging systems are devices
used for the purpose of
measuring position,
momentum, energy, or mass
of charged particles
Pipette bulb volumetric pipettes, and
transfer pipettes can all be
filled manually using pipette
bulbs, also known as pipe
bulbs, pi-petting balls, or
pipette bulbs.
Glass pepitte  small piece of apparatus
which typically consists of a
narrow tube into which fluid
is drawn by suction
Titronic burette  the ideal piston burette for
manual titrations, accurate
dosing applications, and the
preparation of solutions.

Dry block thermostat is a traditional Biosan’s dry

block thermostat for
laboratory analysis.
Thermostat is designed for
maintaining constant
temperature of samples in
tubes inserted 
graduated glass pipette 1ml \Graduated pipettes, glass
Pipettes with a scale
are used to dispense
liquids. They are calibrated
”Ex” (to deliver). The
imprinted volume refers to
the delivered amount of
liquid.
micro-pipette  very fine pipette for
measuring, transferring, or
injecting very small
quantities of liquid.
Normal bin The sharps container medical
Biohazard bin container are designed to permit
Sharp bin safe disposal of sharps and
medical waste. 

tisus is for cleaning hand table

microscope etc

Plastic test tube rack They are especially useful

Wooden test tube rack
for organizing test tubes
when different solutions are
being worked on or
collected at once.
Usually, they are designed to
be 6 or 12 well, which
means they can hold up to 6
or 12 test tubes. Because of
the small volume and little
space occupied, they are
popular in various
laboratories.

Biological safety cabinet is an enclosed, ventilated

laboratory workspace for
safely working with
materials contaminated with
(or potentially contaminated
with) pathogens requiring a
defined biosafety level.
centrifuge  machine with a rapidly
rotating container that
applies centrifugal force to
its contents, typically to
separate fluids of different
densities
Incubator Enclosed apparatus in which
premature or unusually
small babies are placed and
which provides a controlled
and protective environment
for their care.

an apparatus used to hatch
eggs or grow
microorganisms under
controlled conditions.


Vortex mixture  whirling mass of fluid or

air, especially a whirlpool or
whirlwind:
Cell counter  provides a simple way to
perform cell differentials on
a peripheral blood or bone
marrow aspirate
Haematology analyzers Hematology analyzers are
used to conduct a complete
blood count (CBC), which
is usually the first test
requested by physicians to
determine a patients general
health status. A complete
blood count includes red
blood cell (RBC), white
blood cell (WBC),
hemoglobin, and platelet
counts, as well as hematocrit
levels.
5. CONCLUSION
From the Experiment 1, students gain more knowledge about the safety rules that
must be followed by all the students. There are 4 main protections such as
laboratory safety procedures, protective procedures, emergency procedures and
chemical safety. All the protection important and student should be able to
understand and obey all the rules to make sure their safety is always the priority.
For laboratory safety procedures, students learned about responsibility and action
that must be taken when something bad happen in laboratory. We also learned about
cleanliness to protect our body from any hazards that can be harmful for people.
Then, for protective procedures, students learned about all the rules before entering
the laboratory. For example, student should wear gloves, goggles, lab coat and so
on. Therefore, student also learned about the rules when the experiment is in
progress. For example, students are advised to always using the appropriate tools
for each experiment and never eating or drinking in the laboratory during the
experiment.
In the same time, for emergency procedures, students learned about the proper
steps that must have follow in the event of an emergency such as the location of
emergency equipment and their function. We also need to know the emergency
number and the instructor for each laboratory.
Lastly, for chemical safety, student learned about the functions, hazards and label
on each chemical in laboratory and its prevention. Students also learned about how
to use chemical the right way and the procedures that students should follow to be
safe in laboratory.

6. REFERENCE
George H, Wahl, Jr and Maria T. Gallardo-Williams. (2011). Laboratory Safety
Rules and Regulations.
Anne Marie Helmenstine. (June 26,2018). The 10 Most Important Lb Safety
Rules. Retrieved from https://www.thoughtco.com/important-lab-safety-rules-608156
Stony Brook. (2008). Lab Safety Guide. New York. Retrieved
from https://ehs.stonybrook.edu/programs/laboratory-safety/lab-safety-guide