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Qualitative Analysis of Carbohydrate
‘The qualitative analysis of eabohydrate isthe significant test for detecting
and classifying carbohydrates, depending onthe colour change followed by
chemical reactions. Carbohyetateis an organic biomolecule that has 2
chemical formula Cm (HzO) The carbohydrate consists of vee atoms, v2
carbon, hydrogen and exygen asthe main constituent, where the ratio
between the hydrogen and the oxygen is 21.
CCarbohyerates act asthe major energy source for ll ving ergaisms, and
‘hey are primariy of thre kind, namely mano, lige and polysaccharides
Monosaccharides include simple crystaline sugars that are readily soluble
in water, Oligosaccharides include low molecular weight sugars and exist
3 polymer of monosaccharides covaentytnked trough alyeoscie
Linkages.
Polysaccharides incluse high molecular weight sugars and exist ther as 2
polymer ofthe same monosaccharides (homopalysacchardes) or diferent
‘monomers (heterepolyssecharides), In this context we wil discuss the
citferent chemical tests fr the qualitative analysis of carbohyerates.
Content: Qualitative Analysis of Carbohydrate
1. Meaning of Qualitative Analysis of Carbohydrate
2.Video
3. Methods for Qualitative Analysis of Carbohylrate
+ Moliscts Test
Serazicts teat
Feng’ Test
Barfoeds Test
iat Test
Selhwanoft's Test
lodine Test
Meaning of Qualitative Analysis of Carbohydrate
‘The qualitative analysis of carbohydrates is detected based on the
reagent’ utilisation and the action between the test sample and reagent.
‘Tne reaction ofthe test sample with the chemical reagent gives 8
‘Significant colour, through which we can detect the presence or absence of
carbohyrates.
Video: Qualitative Analysis of Carbohydrate
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Methods for Qualitative Analysis of Carbohydrate Chemistry
We can determine the presence of carbohydrates in the test sample by eninneminad
loving te felling harcal tets ctence
oon)
Molisch's Test
ithe most common method forthe detection of carbohydrates. Moles
test makes use of Molisch’s solution {contains a-naphthal in 95% alcohol)
‘and concentrated H,S0,
Principle: Molisch’s test detects the carbohydrate presence, which principle
|s based upon the dehydration reaction. The carbohydrates in the sample
‘et dehydrated into aldehyele by the addition of concentrated HS
‘Tne aldehyde formed i ether furfural (produced by the dehydration of
pentoses or pentosans) or hydroxymethyturfural (reduced by the