Group 3

BIOSAFETY CABINET
AND PERSONAL
PROTECTIVE
EQUIPMENT
INTRODUCTION
BIOSAFETY – it is a practice employed to
protect laboratory personnel and environment
from exposure or infection while working with
living organisms, biological materials, or agents.
BIOLOGICAL SAFETY CABINET – a device that
encloses a workspace in such a way as to
protect workers from aerosol exposure to
infectious disease agents.
 WHAT DO WE NEED TO
KNOW?
The role of the biological safety cabinet can be up to three types of protections:

1. Protection of the worker - from harmful elements inside the cabinet

2. Protection of the product inside the cabinet - to explain contamination at

work, in an experiment or under guidance

3. Protection of the environment - from various pollutants that are created inside
the biological cabinet
BIOSAFETY CABINETS
 BSC CLASS I
Also known as VERTICAL LAMINAR
BSC allows room (unsterilized) air to pass into
the cabinet and around the area.

-air velocity 75 linear feet/min

-exhaust air thru HEPA filter
-negative pressure, ventilated to the outside
 BSC CLASS I
A Class I Biosafety cabinet protects the operator and
the environment from exposure to biohazards. It
does not prevent samples being handled in the
cabinet from being exposed to contaminants that
may be present in room air. Naturally, there is a
possibility of cross-contamination that may affect
experimental consistency. Consequently, the scope
and application of Class I cabinets is limited, and it is
largely considered obsolete.
BSC CLASS II

Sterilize air that flows over the

infectious material, as well as air to be
exhausted.
-no product of contamination
-air velocity 75-100 linear/min
BSC CLASS II

·This class of cabinet is divided into four

types: A1, A2, B1, and B2
BSC CLASS II A2

70% of the air is recirculated through a HEPA filter,

and 30% is exhausted; MOST COMMON BSC USED IN
HOSPITAL CLINICAL MICROBIOLOGY LAB.
BSC CLASS II B1

30% of the air is recirculated through the

HEPA filter, and 70% is exhausted to the
environment.
BSC CLASS II B2

100% of the air is exhausted to the

environment after passing through HEPA
filters.
 BSC CLASS III

Completely enclosed cabinet

-Negative pressure, in and out air filter sterilized,
-rubber gloves is attached to the cabinet
- for BSL 4 VIRUSES
 BSC CLASS III
Class III biological safety cabinets are suitable for work with
microbiological agents assigned to biosafety levels 1, 2, 3 and 4. They are
frequently specified for work involving the most lethal biological
hazards.
Work is performed through glove ports in the front of the cabinet.
During routine operation, negative pressure relative to the ambient
environment is maintained within the biosafety cabinet. This provides
an additional fail-safe mechanism in case physical containment is
compromised. Exhaust air is usually HEPA filtered and incinerated.
Alternatively, double HEPA filtration with two filters in series may be
utilized.
 PERSONAL PROTECTIVE
EQUIPMENT (PPE)
PPE is specialized clothing or equipment
worn by an employee to provide protection
against a hazard (e.g., infectious agents and
toxins). General work clothes (e.g., uniform,
pants, shirts, blouses) are not intended to
function as protection against a hazard and
are not considered to be PPE.
 Personal Protective Equipment (PPE) is specialized
01 clothing or equipment worn by an employee to provide
protection against a hazard (e.g., infectious agents and
toxins). General work clothes (e.g., uniform, pants, shirts,
blouses) are not intended to function as protection
against a hazard and are not considered to be PPE.
 PPE can be as basic as eye protection (safety glasses or
goggles), gloves, and a lab coat or as complex as a
02 Biosafety Level 4 “positive pressure suit” that completely
isolates the employee from the laboratory environment.
The use of specific PPE required is determined through
a risk assessment.
 WHAT IS THE IMPORTANCE OF
PPE IN THE LABORATORY?

Moreover, they have the following

PPE saves scientists from two types of
accidents, radioactive and biological.
Lab safety gear can prevent fatal
injuries and accidents. Most
laboratories put these requirements to
keep the environment healthy and
safe. Personal protection is a matter of
safety and efficiency and should never
be neglected at any cost.
benefits as well.
It reduces the extensive chemical
exposure to the lab technicians.
It improves the quality and
efficiency of lab workers
Some businesses implement these
regulations so their lab can comply
with the latest law requirements. So
in such a scenario, Lab PPE
catalyzes business activities.
 ESSENTIAL LAB SAFETY
EQUIPMENT IN EVERY LAB
01 Face and Eyes Protection

02 Skin and Body Protection

03 Respiratory Protection

04 Head and hearing protection

 FACE AND EYES
PROTECTION

The face or eyes are the closest body parts to getting

any germ or splash in many experiments. In labs,
general safety glasses, laser safety glasses, chemical
splash goggles, and impact goggles are used to keep
them safe. For more safety and protection, advanced
face shields are used so none of the chemical splash or
germs get in contact with scientists.
 SKIN AND BODY
PROTECTION

The most common personal protective equipment (PPE) is

hazmat suits, safety helmets, high-quality goggles, latex gloves,
etc., drafted to protect the wearer's body from injury or infection.
These equipment are needed to save technicians from any
hazards that come from physical, electrical, heat, chemicals,
biohazards, and airborne particulate matter.
 SKIN AND BODY
PROTECTION

A lab coat, gloves, long pants, and closed-toe shoes are the
minimum PPE for work in a laboratory for staff, students, or
volunteers working in a lab where chemical, biological,
radiological, or mechanical hazards are present.
It also includes special knee pads and safety boots that prevent
falling and slipping from any surface, and they are made with a
different material that saves the feet and keeps the worker's
balance intact.
 RESPIRATORY
PROTECTION

Filtering Facepiece Respirators (FFRs) are disposable face masks

that cover the nose and mouth. Elastomeric half-mask respirators
(EHMRs) are reusable respirators that cover the nose and mouth.
Itis a full facepiece recyclable respirator that covers the nose,
mouth, and eyes. PAPRs (Powered Air Purifying Respirators) are
reusable and often have a hood or helmet that covers the nose,
mouth, and eyes.
These different respirators are used when there is a fair chance
that any chemical or fume can cause an uncomfortable situation.
They will cover the nose, mouth, and eyes, and none of the
hazardous particles can affect the human body.
 HEAD AND HEARING
PROTECTION

Hearing protectors are required to prevent noise-induced hearing

loss. These protecting kits protect your ears from any voice, noise,
or radioactive case. Ear muffs, semi-insert earplugs, and earplugs
are the most common types of PPE.
Safety helmets are another standard lab safety equipment to
prevent severe head injury during any experiment. These hard-
made helmets are specially made for industrial and sensitive
research in laboratories.
LIMITATIONS OF
LABORATORY
PPE
 LIMITATIONS

Avoid Incomplete Protection Ensure User Compliance

Whether you are wearing a full latex suit or
vinyl gloves, or an advanced face shield, one
product can protect only one aspect of the
body. The danger can only be mitigated if one
or two things are missing. One must need a
whole set to stay safe from all fatalities.
Ensuring user compliance in the laboratory is
important for maintaining a safe working
environment and preventing accidents or
incidents that could harm individuals,
compromise research outcomes, or pose risks
to the surrounding community.
 LIMITATIONS

Frequent Inspection Possible Interference with Work

All personal protective lab equipment comes Depending on the type of PPE and the nature of
with an expiry date. Whether onsite damage or the task, PPE can restrict mobility, visibility, or
deterioration can make it useless for any agility, potentially impacting the efficiency and
protection. Continuous inspection and kit comfort of the wearer. It can lead to reduced
productivity, increased fatigue, and potential
changes are necessary for lab workers' long-
workplace hazards due to impaired performance.
term safety.
 LIMITATIONS

Environmental Limitations
Heat, humidity, seismic imbalance, and other environmental changes can influence
the performance of certain types of PPE. For instance, protective clothing may
become less breathable or more prone to tears under extreme heat conditions.
Personal Protective Equipment is an indispensable component of laboratory
safety. Lab PPE acts as a barrier between laboratory personnel and potential
hazards, reducing the risk of injuries, exposure to harmful substances, and
contamination. If you want to get these lab supplies online
 PROPER DONNING OF
PPE

GOWN
MASK
GOGGLES OF FACE SHIELD
GLOVES
 PROPER DOFFING OF
PPE

GLOVES
GOGGLES OF FACE SHIELD
GOWN
MASK
PROPER HANDWASH
 REFERENCES

Lecture from Bacteriology Laboratory (Prof Vanessa Ferriol)

https://www.westlab.com/blog/comprehensive-guide-to-lab-ppe-personal-
protective-equipment?fbclid=IwAR0lH13GdFKv5DiQJZN2pAZPt_f-
kt3IfmeZSzdN2oTvR1yhVQd_NwtFWCk
https://www.phe.gov/s3/BioriskManagement/biocontainment/Pages/PPE.aspx
https://www.escolifesciences.com/products/biological-safety-cabinet
https://www.mrclab.com/what-you-need-to-know-about-biological-safety-
cabinets
https://munglobal.com.au/resources/knowledge-base/personal-protective-
equipment/donning-and-doffing-personal-protective-
equipment/#:~:text=Donning%20involves%20putting%20on%20the,face%20prote
ction%2C%20gown%20and%20mask.
MEMBERS

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De Guzman, Kaye
Monzon, Justinne Mika
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Rotazo, Jehiel Grace