Lesson Plan

Recombinant DNA/Grade 12 B Biology

18th January 2019
A. Lesson Objectives
At the end of the discussion, the students are able to:
1. Know the recombinant DNA
2. Explain the process of recombinant DNA
3. Explain the process DNA cloning, make insulin
4. Know application of DNA technologies
5. Know the sanvatage and disadvantage of recombinant DNA
B. Learning Content
1. Topic Recombinant DNA
2. Resources Chalkboard, video, powerpoint, TV, chalk,
3. Methodology Student Center
4. Concepts a. Recombinant DNA
b. The process cloning DNA
c. Application of DNA technology
5. Skill Explain recombinant DNA
6. References - Solomon, Eldra P. Etc. General Biology
- Starr, Evers. Biology : Concepts and
Applications 9e

C. Learning Procedure
1. Apperception/Motivation
- Teacher shows a picture about plant that already using recombinant DNA and
not, next teacher asks the students their insight for the topic that will be
discussed.

2. Lesson Proper
- Teacher introduces recombinat DNA
3. Teacher explain the process of recombinant DNA by power point and also the
sanvatage and disadvantage of recombinant DNA
- Teacher explain the process of cloning DNA by video ex: how to make a dog
with clone, how make insulin
 - Teacher give a game “let’s ask” to the student. Teacher divided the student be
two big group. The group have to make 10 questions about recombinant DNA in
note paper that teacher give. The teacher read the questions that students make
and another group have to answer.
- Teacher give a quiz about recombinant DNA
4. Generalization
Ask some students to summarize the lesson
D. Learning Evaluation
Quizs – directions: answer the questions in your booklet
1. Why recombinant DNA possible to do_____
2. What the name of enzymes that cut in a zig zag pattern____
3. What the name for joining the foreign dna and plasmid____

4. Can be manipulated to carry and clone other pieces of DNA. The function of____
5. Human growth hormone, factor VIII and insulin are the aplication of____
6. What process is being pointed out by the arrow?

Sheep mammary
Enucleated
cell egg cell

Egg cytoplasm containing

donor cell nucleus

7. The most commonly used vectors are___, ___,___

8. What are the three main tools used in recombinant DNA technology?
9. The DNA is inserted into another DNA molecule called____
10. Removal and replacement of defective genes with normal healthy functional
genes is known as ___
11. Restriction fragments from donar DNA are mixed with plasmid DNA and joined
by their ____
12. The exchange of corresponding DNA segments between adjacent chromosomes
during the special type of cell division that results in the production of new
genetic called ____
13. The exchange of corresponding DNA segments between adjacent chromosomes
during the special type of cell division that results in the production of new
genetic called ____
14. This new recombinant virus is used as a ____ in aplication of genetic engineering
(Medicine)
15. Human growth hormone (GH) made by bacteria_____
16. Biologist use tissue culture techniques to isolate individual cells from certain
plants (true or false)
17. The first genetically modified food is corn (true or false)
18. There are benefit for human such as extensive erosion and genetic destruction of
plant Germplasm. (true or false)
19. Golden rice has been genetically modified to contain beta-carotene –This rice
could help prevent vitamin B deficiency

20. DNA fingerprinting

 Has become a standard criminology tool
 Has been used to identify victim
(true or false)