urine. Pregnancy 5. Ballottment - occurs when an egg is fertilized by a sperm, grows - a palpatory technique for detecting or inside a woman’s uterus and develops into a fetus examining a floating object in the body, as a. - In humas, this process takes about 264 days from - The use of a finger to push sharply against the thje date of fertilization of the egg, but he uterus and detect the presence or position of a obstetrician will fate the pregnancy from the first fetus by its return impact. day of the last menstrual period (280 days to 40 weeks) 6. Outlining of the fetal body 7. Goodell’s sign- softening of the cervix Intra- uterine pregnancy 8. Souffle- a low murmuring or blowing sound heard - a normal pregnancy occurs when a fertilized egg is through a stethoscope. implanted in the uterus (womb) and an embryo 9. Braxton Hicks Contraction- an intermittent weak grows. and painless contractions of the uterus occurring during pregnancy. Embryo - the term used for the developing fertilized egg For easy to remember during the first 9 weeks of pregnancy. - To arrange Hegar, Chadwick and Goodell signs, Human Chorionic Gonadotropin (hCG) arrange them anatomically from external to internal - This hormone is secreted by the placenta and can organ, then match them with alphabetical be measured to determine the presence and sequence. progression of the pregnancy. - Where Chadwick is to the vagina. - The presence hCG to rule out the pregnancy can be - Goodell is to the cervix while done through urine or blood tests. - Hegar is to the uterus
Trimester POSITIVE SIGNS: are definitive signs of pregnancy
- the duration of an individual pregnancy is divided into three periods called trimester (approximately FETAL three months in duration) o Heart beat - Each trimester is characterized by specific events ✓ 10 weeks- doppler and developmental markers ✓ 16 weeks fetoscope - For instance, the first trimester includes the ✓ 18-20- auscultation differentiation of the different organ systems. o Movement: felt after 20 weeks o Skeleton- by ultrasonography or X-ra Estimated date of Delivery (EDD) - the delivery date is estimated by counting forward Maternal Physiology During Pregnancy: 280 days from the first day of the woman’s last period. REPRODUCTIVE SYSTEM CHANGES - Also called the estimated date of confinement (EDC) or Expected date of birth (EDB). Uterus - enlargement and thickening of the uterus; marked SIGNS AND SYMPTOMS OF PREGNANCY in the fundus. - At the end of umbilicus by the 20th weeks AOG, Presumptive signs- the changes are felt by women xiphoid process by the 36th weeks; - Descends slight during the last 3 weeks due to the o Morning sickness fetal descent into the pelvis o Amenorrhea – absence of menstrual bleeding o Changes in breast- milk formation starts Cervix o Fatigue - Pronounced softening and cyanosis (due to o Lassitude (lack of energy increased vascularity, edema, hypertrophy and o Urinary frequency hyperplasia of cervical glands) Goodell sign o Quickening (18th-20th weeks)- baby starts to - Cervical plug formed by the thick mucus move (operculum) that stops bacteria from entering the uterus. Probable signs- changes that are observed by the Ovaries- ovulation ceases throughout pregnancy examiner Vagina 1. Chadwick’s sign- bluish discoloration of the vaginal - vascularity, hyperemia, and softening of the wall perineum and vulva. 2. Hegar’s sign- softening of the lower uterine - Chadwick sign noted. Vaginal mucosa in thickness, segment connective tissue loosens and small muscles cells 3. Uterine enlargement- at 12 weeks gestation, the hypertrophy. Vaginal secretions; pH is 3.5-6 due to growth is felt just above the symphysis pubis. production of lactic acid (Doderlein bacillus) Breasts Pituitary - Tender and tingle in the early part of pregnancy - Elevated estrogen and progesterone; suppressed - In size, Larger nipple. More pigmented. LH, FSH, Oxytocin - Areola darker in color - Colostrum present by the 2nd trimester. Elevated CARDIOVASCULAR CHANGES glands of Montgomery (Hypertrophic sebaceous gland) Heart - Colostrum first milk that contains all the nutrients - Heart is deplaced upwards by elevated diaphgram - There maybe splitting of the first heart sound with INTEGUMENTARY CHANGES common systolic murmurs. - Displaced upward to the left and forward Striae Gravidarum - reddish slightly depressed streaks in the abdominal Blood Volume wall, breast and thighs. - Increases by 30-50g/ml Linea Nigra - RBC volume increases by 20-305 - line of dark pigment extending from the umbilicus - Hematocrit decreases by 7& causing physiologic down the midline to the symphysis. anemia Chloasma Pulse Rate- increases by 10-15 bpm - “Masks of pregnancy” are brownish patches on the Supine Hypotensive Syndrome- occurs during the 2nd face. trimester. Pigmentation - pigmentation changes due to melanocyte Circulation - stimulating hormone elevated from the 2 nd month - Cardiac volume increases by 40-50% causing slight of pregnancy cardiac hypertrophy and increase in Cardiac Output. - ex. Chloasma CO increases when the woman turns from her back to her left side. METABOLIC CHANGES - (+) Physiologic anemia - PR increases 10-15 bpm during pregnancy Weight gain - Slight decreases in bp (30%) during the 2 nd and 3rd - weight gain averages is 11-13 kgs (24-28 lbs.) trimester - Fetus (3,400gm); Placenta (450 gm); Amniotic Fluid (900 gm); Uterus (1gm); Breast tissue (1400 gm); Hematologic Blood Volume (1800 gm); Maternal Stores (1800- - Total circulating blood cells increases 3600 gm) - Leukocyte count is elevated during labor - Fibrinogen levels increases by 50% along with other Water Metabolism clothing factors - Average woman retains 6.5 liters of extra water during pregnancy Cardiac Classification in Pregnancy
Protein Metabolism Class I
- Fetus, Uterus and Maternal blood are rich in - Asymptomatic in all activity protein. - No limitation of activity
Carbohydrate metabolism Class II
- Human placental lactogen, estrogen, progesterone - Asymptomatic at rest and insulin produced by the placenta during - Symptomatic with heavy physical activity pregnancy prevent the action of insulin during - Slight limitation of activity pregnancy. - Pregnancy can initiate diabetes and Class III - DM can be aggravated by pregnancy - Asymptomatic at rest - Symptomatic with ordinary activity Fat Metabolism - Able to handle physiologic demands of pregnancy - fats are more completely absorbed during - Considerable limitation of activity pregnancy; plasma lipid levels increase during the2nd half of pregnancy Class IV - Symptomatic with all activity and at rest Iron Metabolism - High risk for pregnancy - iron requirement increases to 20-40 mg daily. - During the first half of pregnancy, iron is transferred GASTROINTESTINAL CHANGES to the fetus and stored in fetal liver. Nausea and Vomiting- common during the 1st trimester ENDOCRINE SYSTEM CHANGES due to increased Hcg Placenta - Produces estrogen, progesterone, human chorionic Ptyalism- excessive saliva in pregnancy due to changing gonadotropin (hCG) and Human Placental Lactogen hormones (hPL), Relaxin Heartburn and Flatulence- often occur dur to reduction Psychological Adaptation of Pregnancy Based on Reva in gastric acidity. Growing uterus and smooth muscle Rubin’s Theory relaxation - Distinct emotional responses of the mother to Heartburn- the hormone progesterone causes the valve pregnancy to relax, which causes First Trimester Flatulence--excessive gas during pregnancy is caused by - Accepting the pregnancy hormone progesterone - Ambivalence: having mixed feeling or - contradictory ideas about something or someone. RESPIRATORY SYSTEM CHANGES Second Trimester Ventilation - Accepting the baby - Hyperventilation occurs increasing respiratory rate, - Fantasizes about the baby tidal volume by 45% - PCO2 lowers. There is a substantial reduction in the Third Trimester partial pressure of carbon dioxide (pCO2) in - Preparing for parenthood pregnancy. - Identification of baby’s appearance
Diaphragm Emotional Aspects of Pregnancy
- enlarging uterus elevates the diaphragm - displacement of the diaphragm causes shortness First Trimester of breath - is denoted by ambivalence having mixed feeling or - nasal stuffiness and epistaxis are common due to contradictory ideas about something or someone edema and vascular congestion induced by - Focus: bodily changes increased estrogent - Developmental Tasks: to accept the biological facts of pregnancy Thoracic cage - expands by means of flaring of the ribs hence Second Trimester increasing the mobility of rib attachments - The mother feels well and happy and fantasies about the baby. SKELETAL SYSTEM CHANGES - Focus: self-growth and development of the fetus - Developmental task: accepts the growing fetus as Hormone Relaxin- relaxation of pubic joints during part of her own pregnancy Third Trimester Lumbodorsal curve- increase in the 3rf trimester - Personal indication of the appearance of the baby. - Focus: o the baby, Reva Rubin Maternal Role Attainment Theory o mother’s delivery process, o responsible parenthood. a) Anticipatory stage: begins ion the pregnancy and - Developmental Task: Preparation for Childbirth includes the initial social and psychological adjustments to pregnancy. The mother learns the Fetal Development expectations of the role, fantasizes about the role, related to the fetus in the uterus, and begins to role - Cephalocaudal in progression play - 1st trimester period of organogenesis b) Formal stage: begins with the birth of the infant and - 2nd trimester period of rapid increase in length includes learning and taking of the role of the - 3rd trimester period of continuous growth and mother. Role behaviors are guided by formal, development consensual expectations and others in the mother’s - Cephalocaudal- refers to growth and development social system that occurs from the head down to the lower c) Informal stage: begins as the mother develops extremities unique way of dealing with the role not conveyed by - Organogensis- the three germ layers of the embryo the social system. The woman makes her ne role fit form into the major organs of the body system within her existing lifestyle based on past experiences and future goals Development at 1-4 Weeks (1 month) d) Personal stage: role identity stage occurs as the - Length 0.75 to 1 cm woman internalizes her role. The mother - Weight 400 gm experiences harmony, confidence and competence - Spinal cord fused at midpoint in the way she performs the role and the maternal - Rudimentary heart appears as a bulge on the role is achieved anterior surface - Extremities appear as buds - Recognizable eyes, ears and nose Development at 5-8 Weeks (2 months) Development at 35-37 Weeks (9 months) - Length 2.5 cm; weight 20 g, - Length 42- 48 cm; Weight 1800-2700g - Completed organogenesis - Body shares glycogen, iron and carbohydrates - Heart with septum beats rhythmically - Calcium deposition - Facial features identifieable - Deposition of subcutaneous fats - Extremities developed - Decrease in lanugo - External genitals present but not clear Development at 38-40 Weeks (10 months) Development at 9-12 Weeks (3 months) - Length 48-52 cm; Weight: 3000 g - Length 7-8cm; weight 45 g - Fetus kick actively - Nailbeds formed formation of bone ossification - Start of conversion of fetal hemoglobin to adult centers - Vernix caseosa fully formed - Tooth buds present - Sole creases cover 2/3 of feet - Heat beat audible by doppler - Lightening - Kidney secretion starts - Maybe present
Development at 13-16 Weeks (4 months) Birth!
- Length 10-17 cm, 55-120g - A mother’s due date marks the end of her 40th week - Fetal heart beat audible by stethoscope - The delivery date is calculated using the frist day of - Lanugo well-formed her last period. - Functional liver and pancreas - Based on this, pregnancy can last between 38 and - Fetus swallow’s amniotic fluid 42 weeks with a full-term delivery happening - Urine present in amniotic fluid around 40 weeks - Sex determined by ultrasound - Some post-term pregnancies those lasting more than 42 weeks- the due date may just not be Development at 17-20 Weeks (5 months) accurate - Length 25cm; 223g. - For safety reasons, most babies are delivered by 42 - Quickening present weeks. - Hair and eyebrows formed - Sometimes the doctor may need to induce labor. - Meconium present in upper intestine - Vernix caseosa forms FETAL CIRCULATION - Definite sleeping patterns develop Structure Location Function Time for an Ultrasound Placenta Attached to the Gas exchange - An ultrasound is usually done for all pregnant uterus during fetal life women at 20 weeks. Umbilical Two arteries in Carry oxygenated - Sex can be determined whether it’s a boy or a girl at arteries the cord blood from the 20 weeks fetus (descending Development at 21-25 Weeks (6 months) aorta) to the - Length 28-36cm; 550g. placenta - Passive antibody transfer Umbilical vein One vein in the Carry oxygenated - Meconium present in the rectum cord blood to the fetus - Start of surfactant production Forman ovale Opening in the To shunt blood - Eyelids open interatrial from the R atrium - Pupils reactive to light septum (between to the L atrium so - Well-defined eyebrows and eyelids R&L atrium) that blood can be - Capable of hearing sounds supplied to the Development at 26-29 Weeks (7months) brain, heart, and - Length 35-38cm; 1200g kidney - Alveoli matures Ductus Accessory vein To supply blood to - Surfactant present in amniotic fluid venosus connecting the the liver. A bypass - Tastes begin tom descend umbilical vein to the fetal liver - Thin retinal blood vessels which are extremely into fetal liver susceptible to damage. and inferior vena cava Development at 30-34 Weeks (8 months) Ductus Connection Shunting of the - Length 38-43 cm; weight 1800-2700g arteriosus between fetal larger portion of - Deposition of brown fat - Fetus responds to external voice lungs and the blood away from - Active presence of Moro reflex aorta the lungs and - Fetus assumes birth position directly into the aorta Embryonic and Fetal Support Structures (after the 2nd gestational month). It is partly responsible for maintaining the corpus luteum Corpus Luteum o First indicator of a positive pregnancy detected in - Supplies most of the estrogen and progesterone in the uterine and plasma (by day 8) the 1st two gestational months before the placenta o HCG levels are also monitored later in the is fully developed pregnancy to determine fetal well-being - Estrogen and progesterone essential for sustaining the uterine endometrium and preventing Human Placental Lactogen (HPL) menstruation o Human chorionic somatomammotropin levels increased after 20 weeks AOG Decidua o It is a growth like substance that stimulates - The endometrium (the lining of the inside of the maternal metabolism uterus) becomes the decidua following fertilization o It facilitates glucose transport acroos the and implantation placenta - The portion under the blastocyst where the o It also stimulates breast development to chorionic villi intersect with the maternal blood prepare for lactation vessels is the decidua basalis - The portion covering the blastocyst is called the Embryonic and Fetal Support Structures decidua capsularis - The portion lining the rest of the uterus is the Membraned and Amniotic Fluid decidua vera - Two membranes form to protect and support the - It will protect and nourish the developing embryo embryo 1. Amnion (inner membrane) encloses the Placenta amniotic cavity, containing the amniotic - The placenta begins to function by the 4th week of fluid and the fetus gestation 2. Chorion (outer membrane) contains the - By the 14th week it is a complete independently amnion and is part of the placenta functioning organ - It transmits nutrients and oxygen to the fetus and Amniotic Fluid removes wastes and carbon dioxide by diffusion - is a clear, yellow fluid which is found within the first 23 days following conception within the amniotic Hormones Produced by Placenta sac. It surrounds the growing baby in the uterus o Placenta - Fluid volume normally ranges from 500-1,000 ml o Estrogen - The functions of the amniotic fluids are the o Progesterone following o Human chronic gonadotropin o Protects the embryo and fetus o Human placental lactogen o Controls temperature o Supports symmetrical growth Hormones of Pregnancy o Prevents adherence to amnion o Allows the embryo or fetus to move within Estrogen the amniotic cavity o It stimulates the growth of uterine muscle (myometrium) and glandular epithelium Umbilical term (endometrium) and induces the synthesis of - at term, it is 30 to 90 cm long and 2 cm in diameter receptors for progesterone - it contains two arteries and one vein o Stimulates uterine growth and uteroplacental - two arteries carry blood from the fetus to the blood flow placenta o Enhances growth of all organs and ensures - one vein returns blood and nutrients to the fetus nourishment of developing tissue - the umbilical cord is normally inserted at the o It indicates placental function, fetal maturity and center of the placenta fetal well-being (levels of maternal serum estriol) - the cord also contains a clear jelly0like substance called Wharton Jelly, which is a connective tissue Progesterone that prevents compression of the blood vessels o Promotes thickening and increased viscosity of cervical mucus (the mucus plug) to protect the Methods of Assessing Pregnancy and Methods of fetus against invading bacteria Estimating EDC o Decreases motility of oviducts and uterus o Stimulates growth of gland breast tissue (acini 1. Indirect/ Non-invasive cells) in preparation for lactation o Maintains uterine lining for implantation a. Nagele’s rule o Relaxes smooth uterine muscle - is a standard way of calculating the due date for a pregnancy. The rule estimate the expected date Human Chorionic Gonadotropin (HCG) of delivery (EDD) by adding one year, subtracting o Secreted by trophoblast cells of the blastocyst three months, and adding seven days to the first (early product of conception) and the placenta day of a woman’s last menstrual period (LMP) b. Fundal height gross estimate of expected of - Urine tests confinement - Blood tests ✓ Complete blood count evaluates - if above the symphysis pubis- between 12-14 weeks hemoglobin/hematocrit and RBC to detect of AOG anemia; - if at the level of the umbilicus – 20 weeks AOG ✓ WBC to detect infection - Fundal height is generally defined as the distance ✓ Platelet Count to assess clotting ability from the pubic bone to the top of the uterus - Blood typing- determines blood type and Rh status to measured in centimeters. After 20 weeks of rule out blood incompatibility pregnancy, the fundal height measurement often - Rubella Titer- detects antibodies for the virus that matches the number of weeks the woman has been causes German measles; titer should be higher than 1:8 pregnant - Hepatitis B- determines if mother has hepatitis B by detecting the presence of hepatic antibody surface 2. McDonald’s rule antigen (HBsAg) - is a measure of size of the uterus used to assess fetal - HIV testing- detects HIV antibodies growth and development during pregnancy - Syphilis Screening- VDRL or RPR serologic tests - it is measured from the top of the mother’s uterus to - Cervical Smears- detects cervical cancer or infections the top of the mother’s symphysis pubis Pharmacologic Treatment Antenatal Care 3. Haase’s rule - Iron supplementation- 60 mg OD - a full-term newborn’s length ranges from 18-22 - Folic acid- 2 tabs daily inches, 9-21 inches or 48-52 cm - Tetanus toxoid- 0.5 ml IM - average length – 50 cm o 1st dose- 1st prenatal check-up Formula: o 2nd dose- 1 months after a. From 1-5 lunar months, square the month of o 3rd dose- 6 months after 2nd dose pregnancy (to square a number is to multiply it o 4th dose- 1 year after 3rd dose by itself) o 5th dose- 1 year after 4th dose b. (months of pregnancy) x (months of In endemic areas give any of the following: pregnancy)= fetal length - Iodized capsule (endemic goiter) - Chloroquine Phosphate (as malaria prophylaxis) Sample Problem A: how long is a 4-month-old fetus? Sol’n: 4 x 4 = 16 cm Obstetric History Terms - Gravida- state of being pregnant; total number of Sample Problem B: What is the estimated length of a 3- pregnancies including present pregnancy month-old fetus? - Para- number of pregnancies reaching the age of Sol’n: 3x3= 9 cm viability whether born alive or not - G- gravida; the current pregnancy Direct/ Invasive - T- the number of full-term children born (children born - Ultrasonography 37 weeks and beyond) - Laboratory findings/ deviations - P- number of preterm children (born before 37 weeks - Urine tests AOG) - Blood tests - A- abortions; the number of pregnancies ending before 4. Bartholomew’s Rule 20 weeks AOG c. Bartholome’s rule of 4s- measures AOG by - L- number of living children determining the position of the uterus in the - M- multiple pregnancies abdominal cavity d. This method estimates the age of gestation relative Vaccine Administration During Pregnancy to the height of the fundus of the uterus above the - Vaccines to be considered unless otherwise symphysis pubis contraindicated o Hepatitis B Antenatal Care o Influenza (inactivated) Prenatal visits o Tetanus o 1st trimester- every 4 weeks (monthly) o Meningococcal o 2nd trimester- every two weeks (twice monthly) o Rabies o 3rd trimester every week (4 x a month) - Vaccines absolutely contraindicated during pregnancy o Philippine DOH Guideline: 80% of pregnant o Influenza (live attenuated) women should have at least 5 prenatal visits o Measles o Mumps Assessment of Risk Factors o Rubella o Age o Varicella o Gynecologic history Diagnostic Tests to Evaluate Fetal growth and Well- o Underlying medical conditions being o Sexually transmitted infections o Teratogenic drugs substance abuse Diagnostic Tests and Procedures (DOH) Standards Daily fetal movement count (DFMC) - Assess fetal activity Amniocentesis - Noninvasive test done by woman who is pregnant - Five to 10 movements per hour; normal activity - Invasive procedure for amniotic fluid analysis to - Five movements or less per hour may indicate fetal assess fetal lung maturity or disease; done after 14 threat or sudden change in movement pattern weeks of gestation - Assess for fetal sleep patterns - Needle placed through abdominal-uterine wall; designated amount of fluid is withdrawn for Nonstress Test (NST) examination - Empty bladder if gestation greater than 20 weeks - Correlates fetal movement with FHR. Requires - Possible complications: onset of contractions; electronic monitoring infections (probably amnionitis); placental - Reactive test- three accelerations of FHR to 15 punctures; cored puncture; bladder of fetal puncture beats/min above baseline FHR, lasting for 15 seconds - Advise women to observe and report the following to or more, over 20-minute time period physician: fetal hypoactivity or hyperactivity, vaginal - Nonreactive test- no accelerations or acceleration bleeding, vaginal discharge (clear or colored), signs of less than 15 beats/min above baseline FHR. May labor, signs of infection indicate fetal danger Vibroacoustic stimulator (VAS) to Analysis of Amniotic Fluid differentiate hypoxia from fetal sleep - Unsatisfactory test- data that cannot be interpreted - Chromosomal studies to detect genetic abberations or inadequate fetal activity; repeat - Biochemical analysis of fetal cells to detect inborn - Contraindication stress test (CST); oxytocin challenge errors of metabolism test (OCT) - Determination of fetal lung maturity by assessing - Correlates fetal heart rate response to spontaneous lecithin/sphingomyelin ration or induced uterine contractions - Evaluation of phospholipids; aids in determining lung - Requires electronic monitoring maturity - Indicator of uteroplacental sufficiency - Determination of creatinine levels; aids in - Identifies pregnancies at risk for fetal compromise determining fetal age. (greater than1.8 mg/dL from uteroplacental insufficiency indicates fetal maturity and the fetal age.) - Increasing doses of oxytocin are administered to - Assesses isoimmune disease stimulate uterine contractions until three in 10- - Presence of meconium may indicate fetal hypoxia minute period - Interpretation: negative results indicate absence of Chorionic villus sampling (CVS) late decelerations with all contractions - Positive results indicate FHR decelerations with - Cervically invasive procedure contractions - Advantage- results can be obtained after 10 weeks of - Nipple stimulations (breast self-stimulation test) may gestation due to fast-growing fetal cells also release enough systemic oxytocin to contract - Procedure- removal of small piece tissue (chorionic uterus to obtains CST. Instruct not to do at home villus) from fetal portion of placenta. Tissue reflects genetic makeup of fetus Biophysical profile (BPP) Health Promotion During Pregnancy - Observation by ultrasound for four variables for 30 minutes and results of nonstress testing: - Bathing- daily bath or shower is recommended - fetal body movements because of increased sweating and vaginal discharge. - fetal tone Leukorrhea is thick, whitish or yellowish vaginal - amniotic fluid volume discharge due to estrogen imbalance - fetal breathing movements - Breast Care- wear firm supportive brassiere to prevent - Variables are scored at 2 for each variable if breast discomfort; wash breast with clear tap water to present, score of 0 if not present; score of less than remove colostrum secretion if present 6 is associated with perinatal mortality - Dental care- continue good brushing habits throughout - Ultrasound pregnancy especially because gingival tissue o Noninvasive procedure involving passage of hypertrophy usually occurs high-frequency sound waves through - Sexual activity- although there is a decrease in the uterus to obtain data regarding fetal desire for coitus in the woman, there are no restrictions growth, placental positioning, and the unless there is a history of preterm labor or history of uterine cavity spontaneous abortion - Purpose may include: - Exercise- exercise should be maintained at 70-85% of o Pregnancy confirmation their maximum heart rate. This is important to prevent o Fetal viability circulatory stasis in the lower extremities o Estimation of fetal age - Sleep- may need rest periods during the afternoon in modified Sim’s position - Employment- no contraindications during pregnancy unless there is exposure to toxic substances, lifting of heavy objects, excessive physical strain, long periods of standing or having to maintain body balance - Travel- in early pregnancy, there are no restrictions; in the late pregnancy, advise the pregnant woman to take frequent rest or stretch during long trips
Expected Weight Gain During Pregnancy
- First trimester- 1lb. a month
- Second trimester- 1 lb. a week - Third trimester- 1 lb. a week
Nutritional Requirements During Pregnancy
Nutrient Daily Requirement for
pregnant women calories Additional 300, in second and third trimesters Calcium 1200 milligrams folate 600-800 micrograms iron 27 milligrams