ABSTRACT

Mandala Waluya College of Health Sciences Kendari DIV

Study Program for Medical Laboratory Technology
Thesis, 3 August 2019

WA NUR ARLIN RAHMADHANTY (A201501042)

EFFECTIVENESS OF JAVA ACID FRUIT EXTRACT (T. indica L.) ON THE
CALCULATION OF LEUKOSIT AMOUNT DIRECT METHOD
Supervisor I: Nursidah, SKM., M. Ked
Supervisor II: Titi Purnama, S. Si., M. Kes
XVIII + 63 + 17 + 9 + 9
Laboratory tests that are often carried out are blood tests, one of which is
counting leukocytes. Leukocytes are counted per unit volume of blood by making
dilutions of blood using Turkic solutions. Turkic solutions are often unavailable or
expired, so research is conducted to look for alternative reagents. The purpose of this
study was to determine the effectiveness of variations in the concentration of extracts
of tamarind (T. indica L.) on the number of leukocytes.
This type of research is experimental research conducted at the STIKES
Mandala Waluya Kendari Integrated Clinics Laboratory. The number of samples is as
many as 24 counting rooms with manual inspection methods using counting rooms
that are examined under a microscope. The data obtained were analyzed by simple
linear regression test. The results showed that of the 24 dosage rooms, the average
number of leukocytes in the control solution was 9,208 cells / mm3, the concentration
of Javanese acid extract was 50% with an average yield of 8,408 cells / mm3, the
concentration of Java extract was 70% with the average yield was 6,391 cells / mm3,
and Javanese acid extract concentration was 100% with an average of 5,983 cells /
mm3.
Hypothesis test results show that the sig value is 0,000 <α (0.05), so there is an
effective concentration of tamarind fruit extract (T. indica L.) on the direct method of
leukocyte count, H1 is accepted and H0 is rejected. Based on the results obtained,
the extract of Java tamarind concentration of 50% is the most effective concentration
to replace glacial acetic acid in Turk solution. Suggestions for future researchers to
use lower concentration variations and pH variations to determine the most effective
concentration and pH.
Keywords: Javanese Fruit Extract, Variation of Concentration, Calculate
Leukocyte Number
Bibliography: 33 (2003–2018)