RAMOS, NINA RICA R.

PARA-LEC MEDTECH 3YA-2

Week 3: Summary Table of Amoeba

Tabulate the following species under subphylum Sarcodina based on the following criteria:

PROTOZOAN SPECIES "CYST"

Protozoan species Size Number of nuclei Karyosome Peripheral chromatin Cytoplasm and
"cyst" inclusions

E. histolytica 8-22 um one to four small and central fine and evenly distributed finely granular/
chromatid bars:
rounded ends (cigar-
like) on young cyst,
diffuse glycogen mass
in young cyst

E. coli 8-35 um one to eight large, irregular shape unevenly distributed coarse and
eccentric granulated/ diffuse
glycogen mass
present in young cyst
thin chromatoidal
bars w/ pointed to
splintered ends in
young cyst

E. hartmanii 5-12 um one to four small and central fine and evenly distributed finely granular/
 chromatoidal bars w/
rounded ends in
young cysts, diffuse
glycogen mass in
young cyst

E. nana 4-12 um one to four large, blot-like, usually absent granular and
central vacuolated/
chromatin granules
nondescript small
mass, diffuse
glycogen mass in
young cyst

E. gingivalis / / / / /

I. butschlii 5-22 um one large, eccentric absent coarsely granular and

achromatic granules vacuolated/ well-
on one side may be defined glycogen
present mass, granules may
be present

Naegleria fowleri 5-22 um one large, eccentric, absent coarsely granular and
achromatic granules vacuolated/ well-
on one side may be defined glycogen
present mass, granules may
be present

Acanthamoeba 8-25 um one large and central absent diorganized, granular,

spp. sometimes
vacuolated/ double
cell wall, smooth
 polygonal inner cell
wall and outer
wrinkled/jogged cell
wall.

B. coli 43-66 um two / / one or two

contractile vacuoles
(1) kidney shaped in young cyst, double
(macronucleus) cyst wall: raw of cilia
(2) small spherical visible in between
(micromolecules) cyst wall layers of
young cyst

E. polecki 10-22 um one small and central fine and evenly granular/
distributed chromatoidal bars,
angular painted
ends in young cyst

glycogen mass in
young cysts,
inclusion mass

PROTOZOAN SPECIES "TROPHOZOITES"

Protozoan Size Motility Karyosome Peripheral Cytoplasm Pathogenicity Unique feature

species chromatin and inclusions
"trophozoite"

E. histolytica 8-65 um progressive, small and fine and finely 1.) Intestinal amebiasis Virulence factors:
directional, evenly granular/
Culture: one finger-like central distributed ingested red a.) Amebic colitis/ Amebic 1.) Suppressor
Robinson's and pseudopodia blood cell dysentery: abdominal pain, factor: neutralizes
Inaki medium diarrhea stomach pH

b.) Characteristic ulcer: FLASK 2.) Gal/Gal NAc

SHAPE ULCER lectin:
cytoadherence
c.) Ameboma - mass-like lesion
with abdominal pain and 3.) Amebapores:
dysentery (<1%) poreformers

2.) Extraintestinal amebiasis 4.) Cysteine

proteinase: tissue
a.) Hepatic ambiasis: Amebic invasiveness
Luver Abscess: ANCHOVY
SAUCE-LIKE EFFUSION "Schaudinn"

b.) Pulmonary amebiasis Emily Brumpt:

c.) Cerebral amebiasis
d.) Amebic pericarditis
e.) Cutaneous amebiasis
differentiate E.
dysenteriae (E.
histolytica) to E.
dispar

f.) Genital amebiasis

E. coli 12-55 non- large, unevenly coarse and can cause harm to larger than E.
um progressive or irregular distributed granulated/ immunocompromised patient/ histolytica
sluggish, many shape vacuoles
blunt/ eccentric containing asymptomatic
rounded bacteria often
pseudopodia visible
E. hartmanii 5-15 um non- small and finely and finely asymptomatic small race of E.
progressive, central evenly granular/ histolytica
finger-like distributed ingested
pseudopodia bacteria may
be present

E. nana 5-12 um sluggish, non- large, absent granular asymptomatic smallest intestinal
progressive, irregular, vacuolated/ amoeba
blunt blot-like bacteria
pseudopods (ink) "cross-eyed cyst"

E. gingivalis 8-20 um active, varying centrally fine and finely specimen of choice: mouth located on mouth
pseudopod located evenly granular/ scrapings (gingival areas),
'only amoeba that
appearance distributed LEUKOCYTES vaginal and cervical materials
epithelial cells, has NO CYST
Pyorrhea alveolaris: pus cell on STAGE'
bacteria, food
particles mouth

*prompt removal of IUD's in

infected patients spontaneously
removes E. gingivalis from the
genital tract

I. butschlii 8-22 um sluggish, large, absent coarsely asymptomatic "iodine-loving"

usually eccentric granular and
progressive achromatic vacuolated/ Iodine stain:
MAHOGANY
granules bacteria, yeast
may or may cell and other BROWN
GLYCOGEN
not be debris
present VACUOLE

Naegleria 8-22 um sluglike, blunt large and absent granular, FREE LIVING PATHOGENIC "true pathogen"
fowleri pseudopods usually usually AMOEBA (FACULTATIVE) Other species:
central vacuolated
(lobate: located asymptomatic N. gruberi: most
lobopods) commonly used
PAM: Primary Amebic nonpathogenic
Meningoencephalitis spp. for research
specimen of choice: CSF N. austreliensis:
Kernig's sign pathogenic to mice

v N. philippinensis:
|__o pathogenic to mice
• pleocytosis (high percentage but only woth high
of PMN cells) doses

• hypoglycorrhacia

• elevated protein levels

Culture: Balamuth medium

Treatment: Amphotericin B

stagnant water: thermophilic

Acanthamoeba 12-45 sluggish large absent granular and • reported from many countries opportunistic
spp. um (slower), vacuolated worldwide both CNS and eye
spinelike infection Species:
pseudopods A. catellanii - most
• appears in
immunocompromised patient common specie
that cause infectio
• isolated from air aquaria, to human
bottled mineral water, soil,
Also A. culbertsoni
swimming pool, deep well
water, contact lens solution

GAE: Granulomatous amebic

encephalitis (Stamm: 10 days)

Acanthamoeba keratitis (eyes)

B. coli 28-152 rotary, boring / / one or two Balantidiasis, balantidial largest

um in (directional, visible dysentery
common to pigs
length, tumbling, contractile,
22-123 rolling ball) vacuoles Enzyme: hyaluronidase - tissue
invader
um wide cytoplasm
may contain ROUND BASE AND WIDE NECK
food vacuoles
or bacteria
small
cytostome
present
 Layer of cilia
around
organism

E. polecki 8-25 um Normal stool: small and fine and granulated common to pigs
sluggish non- central evenly and and monkeys
progressive distributed vacuolated/
ingested sometimes became
Diarrheal accidental parasite
bacteria, other
stool: food particles to humans
progressive
unidirectional

PROTOZOAN SPECIES "VACUOLATED FORM"

Protozoan species Size Vacuole Cytoplasm Nuclei

"vacuolated form"

B. hominis 5-32 um centrally located, fluid-filled appears as a ring around two or four located in
structure consumes almost 90% of periphery of organism cytoplasm
organism