SAN FERNANDO, PAMPANGA

Name: JOHN PAOLO BAYRO LACANILAO (04210003511)

Section: MEDTECH 2-Y1-IRREG
Subject: CLINICAL BACTERIOLOGY – LABORATORY

LABORATORY REPORT: ASEPTIC TECHNIQUE & INOCULATION OF BACTERIA

Guide Questions.

1. What kinds of organisms are destroyed when your desktop is scrubbed down with a
disinfectant?
ANSWER: Vegetative cells & viruses
In the vegetative cycle of viral infection, multiplication of progeny viruses can be rapid.
This cycle of infection often results in the death of the cell and the release of many virus
progeny. Certain viruses, particularly bacteriophages, are called temperate (or latent)

2. Are bacterial endospores destroyed?

ANSWER:
While significantly resistant to heat and radiation, endospores can be destroyed by
burning or by autoclaving at a temperature exceeding the boiling point of water, 100 °C.
Endospores are able to survive at 100 °C for hours, although the larger the number of hours the
fewer that will survive.

3. How hot should inoculating loops and needles be heated?

ANSWER:
Heat the inoculating loop in the Bunsen burner until it is red hot. Heat several inches of
the loop, since that much of it will contact the inside of the tubes. Allow the loop to cool for a few
seconds while you hold it in your hand. Do not put it down or allow the loop to touch any surface
after it is sterile.

4. Why is it necessary to flame the mouth of the tube before and after performing an inoculation?
ANSWER:
Flaming the Mouth of the Test Tube: Passing the mouth of a tube through the flame of a
Bunsen burner creates a convection current which forces air out of the tube. This prevents
airborne contaminants from entering the tube.

5. Why is it important not to dig into the agar with the loop?
ANSWER:
The bacteria can grow in teh gouge preventing the bacteria from growing into distinct colonies.

COLLEGE OF MEDICAL LABORATORY SCIENCE

6. Why must the loop be flamed before entering a culture?
Why must it be flamed after making an inoculation?
ANSWER:
The loop is flamed before entering a culture tube to ensure that no contaminating
microbes are introduced into the culture. The loop is flamed afterward so that no culture
microorganisms are introduced into the working environment.

COLLEGE OF MEDICAL LABORATORY SCIENCE