LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria

Guide Questions.
1. What kinds of organisms are destroyed when your desktop is scrubbed down with a
disinfectant?
 Applying disinfectant to the table top can destroy vegetative cells viruses and
other microorganism.

2. Are bacterial endospores destroyed?

 No, because Endospores is very difficult to kill due to its tough bacterial
structure.

3. How hot should inoculating loops and needles be heated?

 Heat the inoculating loop and needle until it is red hot. But base on my research
the temperature of the heat requires 250 degree Fahrenheit.

4. Why is it necessary to flame the mouth of the tube before and after performing an
inoculation?

 It is important to heat the mouth before performing inoculation to prevent

contamination to the agar.

5. Why is it important not to dig into the agar with the loop?
 Digging to the agar can destroy the bacterial colony.

6. Why must the loop be flamed before entering a culture? Why must it be flamed after
making an inoculation?
 It is flamed to sterilized to prevent contamination to the agar and it must be
flamed after inoculation to kill the microorganism in the loop from the agar.