Professional Documents
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SERVICE MANUAL
BRIO 2
BASIC ROBOTIC IMMUNOASSAY OPERATOR
SERVICE MANUAL
Brio 2 version 1.10 and following
SEAC S.R.L.
Via di Prato, 72/74 - Calenzano – Florence - ITALY
Tel: +39 055 8877469 - Fax: +39 055 8877771
Code 53872940
Revision Date 28/11/07
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BRIO 2 Automatic Preparator - Service Manual
INDEX
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BRIO 2 Automatic Preparator - Service Manual
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I. RESPONSIBILITY DECLARATION
SEAC declines all responsibility for damage due to any type of modifications made to the
Hardware or Software and damage due to connections with other instruments not carried
out by our personnel or previously authorized.
In the event of the device being damaged do not switch it on until it has been repaired by
our personnel.
All operations of an electrical nature required for installing or repairing the instrument must
be carried out by our personnel; avoid all other types of installation.
The instrument must be installed in suitable environmental conditions as described in
points II and III of this chapter, in order to ensure that the performances of the instrument
comply with those illustrated in the technical specifications.
The BRIO 2 Robotic immunoassay operator guarantees maximum safety for the operator
during operation. Nevertheless, as there is dangerous internal voltage, the operator must
follow the general safety rules:
∗ Make sure that the Power Supply has the required voltage before switching on the
instrument.
∗ The instrument must have a ground connection. In case of an electric short circuit the
ground connection eliminates the danger of electric contact with the external metal
parts.
∗ In case of a current surge (due to a short circuit or another malfunction within the
instrument or due to sudden line voltage variations) the protection fuse located on the
back will intervene; in this case, if the new fuse burns out again after having replaced it
with another of the same kind, the instrument must be disconnected and the Service
Technicians must be contacted.
∗ Do not use the commands or adjustments inside the instrument and notify the service
staff as soon as any anomaly occurs during functioning.
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BRIO 2 Automatic Preparator - Service Manual
∗ Before starting any type of intervention inside the instrument switch it off and
disconnect the power cable. Switch the instrument off from the Main Switch. (Press O
to switch off). Unplug the power cable to disconnect it; do not pull the cable.
∗ Any electric operation that is necessary to the installation must be carried out by
qualified staff.
∗ If the device is damaged do not turn it on until it has been repaired by a technician from
the Manufacturer’s Assistance Service.
Disturbances on the power line may be caused by several factors such as:
We must stress that serious power supply line disturbances have negative effects on the
system.
An efficient ground connection is necessary to reduce line disturbances and guarantee the
maximum safety to the operator.
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BRIO 2 Automatic Preparator - Service Manual
TABLE OF SYMBOLS
1 Alternating Current
2 Functional ground
3 ON
4 OFF
5 Fuse
8 CE Mark
9 Biological hazards
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BRIO 2 Automatic Preparer - Service Manual
1. GENERAL DESCRIPTION
1.1. INTRODUCTION
The BRIO 2 device is a high speed automatic system for 96 wells microplates preparing
for immunoenzymatic and Allergologic assays.
The versatility of the system is exalted by the simple and intuitive management software
which, thanks to a special series of options, greatly simplifies intervention by the operator.
The BRIO 2 management program is a high-performance software that is carried in
Windows XP. This is characterized by a User-program interaction based on the dialogue
and menu windows displayed on the screen which guide the operator in the correct work
procedure.
The main menu also includes utilities of a general nature and a special function entirely
dedicated to technical assistance.
The software is able to simultaneously manage the preparation of various tests calculating
the times in order for the time foreseen by the method for carrying out the test to be
complied with. The reading of the test is carried out via internal microplate reader.
The possibility exists of automatic recovery from errors generated by the electric power
fault or any other type of malfunctioning like an interruption in the electric energy.
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BRIO 2 Automatic Preparer - Service Manual
The conducting of tests with a high risk of interserum entrainment is reliable thanks both to
the introduction of interserum washing equal to 5000 µL (two syringe-loads of 2500 µL)
during the analysis Editing phase and with an extension of up to 2500 µL of the washing
volume between the repeats of the same serum during the parameter-setting phase, and
also thanks to the possibility of carrying out an SMP priming with a serum rate equal to the
dispensing one.
It is possible to carry out a simulation of the preparation of the EIA tests or the CARLA
dosage thus passing directly onto the reading phase.
There is also the possibility of correcting the reports and exporting the data to an ACCESS
format in order to allow for the use of this data with other analyzing programs.
It is possible to set the language on which to base the interaction program. In this case the
description of the controls and the controls themselves will be displayed in the language
set.
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Dispenser unit
Syringe : 2500 µL
Syringe : 1000 µL
Predilution
Consisting of the above-mentioned elements. All the dilutions programmed are possible
as long as the volume of serum higher than 10 µL is always used.
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BRIO 2 Automatic Preparer - Service Manual
General features
Distribution : 1 phase, neutral and earth
Consumption : 810VA
Operating conditions
Temperature : 15 ÷ 32 °C (on)
0 ÷ 50 °C (off)
Altitude : <2000 m
Environmental coefficient : 2
Dimensions : 44 x 111 x 60 cm
Weight : 80 Kg
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BRIO 2 Automatic Preparer - Service Manual
The following figure shows a summarizing scheme of the instrument functional parts.
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BRIO 2 Automatic Preparer - Service Manual
1.4. DEVICES
All the instrument devices are identified in the following table which gives the user a brief
description of their function. The listed devices are marked with a character that identifies
them in Fig. 1.6.
7 Rack boards This houses the electronic power supply and control boards
8 Monitor Allows for displaying all the information coming from the
internal PC.
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All the devices and the electrical connections which are on the back panel (Fig. 1.8) are
listed in the following table which gives the user a brief description of their function.
instrument itself.
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The following table lists all the devices and the hydraulic connections which are on the
back panel. It gives the user a brief description of their function. All the listed devices are
marked with a number and a name that identify them either in Fig. 1.9 or the instrument
itself.
Note: The hydraulic Connector and the Level Sensor relative to the same element have
the same Reference Number.
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BRIO 2 Automatic Preparer - Service Manual
1.7. HYDRAULIC
1.7.1. FOREWORD
Make sure there are no air bubbles inside the hydraulic circuit in order to guarantee its
correct function. This is possible by driving the 4 solenoids and the syringes in the
appropriate manner during the priming cycle before starting work.
It also includes:
– Flush solution external tank
– external container of the external needle washing liquid
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BRIO 2 Automatic Preparer - Service Manual
Consists of:
– External tanks for the washing solution (from 1 to 2)
– Peristaltic pump
– N.C. valve placed on the mobile arm
– 1 2-way valves for switching one the 2 tanks on the manifold feed.
– manifold
The N.C. valve which is near the manifold allows the system to obtain the maximum
accuracy in distribution (it opens after the peristaltic pump pressurized the duct) and it
prevents liquid emission from the manifold during the mobile arm movements.
The waste tank is kept at negative pressure (-300 mbar) by the external vacuum pump.
For correct operation of the preparation unit it is very important that there be no leaks in
the vacuum circuit. Make sure the waste tank cap has a perfect seal.
The external WASTE tank is connected to the back panel through 2 tubes;
Tube D
This is connected to the 2-way switchover vacuum valve, normally connected to the
aspiration tubes of the manifold and the external needle washing chamber; from where the
washing solution is aspirated that comes from the external-needle washing peristaltic
pump (Needle Washing Pump).
The other valve way is connected to the manifold feeding circuit and allows it to be
emptied after having partially recuperated the feeding solution by the peristaltic pump.
This permits the use of a minimum quantity of solution for the circuit start-up every time the
user changes the washing solution tank.
This system allows for limiting any waste of washing solution during rinsing.
Tube C
This is connected to the recovery tank of the internal needle washing solution and the
manifold rinsing tank. (Washing Bachwater Tank).
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BRIO 2 Automatic Preparer - Service Manual
No. Description
1 Syringe unit
2 Electromagnet
3 Magnetic sensor
4 Monitor (not present in S model)
5 Inverter board (not present in S model)
6 PC power supply (not present in S model)
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A
3
Vedi dis. B
5
6
10 11
E
12
11
12
I
Vedi dis. C
7 8
13-14
Vedi dis. C I
Vedi dis. B E
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No. Description
1 Motherboard
2 Pliers control board
3 X motors control board
4 Power supply board
5 CPU board
6 Y motors control board
7 Syringe 2500 µL
8 Syringe 1000 µL
9 Belt
10 Microswitch
11 Solenoid valve open normally
12 Solenoid valve closed normally
13 Teflon tip 2500 µL
14 Teflon tip 1000 µL
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No. Description
1 Sensor
2 Sensor
3 Needle washing chamber
4 Stepper motor
5 DC motor
6 Needle
7 Solenoid valve
8 Manifold
9 Needle sensor board
10 Sensor
11 Needle control
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No. Description
1 Spring
2 Peristaltic pump tube
3 Vacuum deviation solenoid
4 Transformer
5 Shaker motor
6 NTC
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No. Description
1 Pliers unit
2 Motor
3 Motor
4 Gear
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No. Description
1 Fan
2 Power Supply Board
3 CPU Board
4 Transformer
5 Analogue Board
6 Sensor position
7 Filter 405 nm
8 Filter 450 nm
9 Filter 492 nm
10 Filter 540 nm
11 Filter 620 nm
12 Belt
13 Motor
14 Belt
15 Filter IR
16 Lamp
17 Fiber Optics
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No. Description
1 Flushing/washing external needle tank plug
2 Flushing/washing external needle tank
3 Plate washing solution tubes
4 Waste tank
5 Waste tank plug
6 Level connector for flushing/washing external needle tanks
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The microplate that is ready for readings is positioned by the pliers on this feeder.
Using the belt, the motor drags the feeder under the photometer’s optical beams.
The position of the Home Position sensor must coincide with the magnet positioned on the
feeder when the feeder is in mechanical end strike to the far right.
Two springs are present on the two sides of the feeder, which guarantee the stability of the
microplate during the reading.
The sensor allows the measuring of the feeder’s initial position.
There are 8 holes on the left part of the feeder, which have the job of synchronizing the
sampling of the photometric signal in order to measure the centre of the wells with the
mean.
The photometric samples are saved every time the stepper motor passes and every 0.9
mm exactly. In total, the microprocessor performs 18 samples for each well; 9 at the centre
and 8 off-centre of the well. During the feeder’s shift for each of the 8 photometric
channels, 18 samples are therefore taken for each of the 12 wells on the row, therefore
18x96 samples overall.
At the end of the shift, the motor inverts the sense of rotation and the feeder is therefore
brought back in line with the Home Position sensor.
The above-stated 8 holes in the left part of the feeder also guarantee that the level
including the 8 optical beams is at right angles to the direction of movement of the
microplate feeder. This is possible as the microprocessor measures the instances when
the photometric signals exceed a certain threshold value. If the photometric unit isn’t at
right angles to the feeder’s motor, the maximum values of these signals will not coincide
over time.
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This consists of a 12 Volt and 20 Watt halogen lamp, a Filter IR and a system of 8 optical
fibers facing the lamp.
At the opposite end, there are 8 lens which allow the light ray to focus around the centre of
the microplate well. There are another 8 lens under the feeder, which focus at the centre of
the well and consequently allow there to be parallel outgoing beams. After the interferential
filter, these parallel beams come into contact with another 8 lens that focus the beam on 8
photometers.
The power supply voltage of the lamps is made stable and controlled by a microprocessor
in order to optimize the photometric signal after the chosen filter has been inserted. The
lamp’s voltage is regulated in such a way that the output of an analogue/digital converter
of the analogue board is less than 4000. Being 12 bit, this converter will indeed have a
maximum output value of 4096. If a value higher than 1200 also isn’t obtained at the
maximum power supply voltage of the lamps, there is an error. If a value lower than 4000
also isn’t obtained at the minimum power supply voltage of the lamps, this also indicates
an error.
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All the electronic processing circuits of the analogue and digital signals, the activation of
the motors and devices are assembled on a board, which is housed in a cradle and
connected to the general wiring with a bus equipped with connectors.
The board cradle is situated in the upper part of the instrument. The board cradles are
listed as follows with the functions performed.
No. Description
1 CPU board and continual motor control
2 Power supply board
3 Axis Y motor control board
4 Axis X motor control board
5 Pliers control board
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All the power supply circuits are on this board, providing the following functions:
a) +12V – 12V
Power supply regulated for the analogue part. The parts concerned are from D15 to D18
bridge rectifier; C1, C2, C11, C12, C14, C17, C18, C19 filter condensers; U2 and U6
regulators
b) +5V
Power supply regulated for the logistical part. The parts concerned are from D1 to D4
bridge rectifier; C7 to C10 filter condensers; U3 regulators.
c) +36V
Power supply not power regulated for the power supply of the controls for the activation of
syringes and sampling needle motors, continual motors and solenoid valves. The parts
concerned are from D5 to D8 bridge rectifier; C3 filter condenser.
d) +50V
Power supply not power regulated for the power supply of the controls for the activation of
the X and Y axis motors. The parts concerned are D14 bridge rectifier; C4 filter condenser.
e)
Power shutdown circuit that intervenes when switching the instrument on and off to
provide delayed power voltage compared to other power supplies to avoid shock to the
motor control parts.
f) Temperature control
The temperature is controlled by a NTC. The resistances R35, R36, R38 and R39 create
the NTC linearization circuit.
g)
Circuit to control vacuum pump and washing pump
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There are 2 stepper motor control boards (X axis and Y axis boards) which are identical
apart from the U5 integrated software.
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6809
Includes the microprocessor MC6809 (U9), the memory decoding circuit and I/O
(U11), the RAM (U16) and EEPROM (U10).
The microprocessor MC6809 interprets the incoming information from the PC,
executes its competent commands and sends the commands to their destination to the
motor control board.
Includes the section (U14, U18, U9) for the reception and data transmission from and
to the PC creating an asynchronous serial interface RS232 without handshake signals,
with a transmission speed of 9600 baud, without parity control, 8 bit and a start bit
(9600,N,8,1).
Includes the 6522 (U13) by which the microprocessor manages the following logics:
Outgoing:
BUZZER
The buzzer used as an acoustic alarm.
A/I_PUMP8
Selects the operating mode of the peristaltic pump of the microplates’ washing section.
Down sets the peristaltic in operation, high in emptying.
MIXER
If low (0V) activates the shaking of plate 1.
E.V.VACUM
Activates, if high (5V), the switchover vacuum valve.
E.V.MANIFOLD
Open, if high (5V), the N.C. manifold valve.
CSEEPROM
Selects the outgoing EEPROM.
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DATAIN
Serial for data transmission from MC6809 to serial EEPROM.
CLKEEPROM_AD
Synchronising clock of data being sent and received with the serial EEPROM.
OPEN2
If high (5V), switch the selection valve of the washing solution.
PLI.LIFT_DOWN
If high (5V), enable the run of the pliers.
OPEN_PLIERS
If high (5V), enable the opening of the pliers.
Incoming:
8NEEDLES_HOME
Low (0V) when the manifold is high.
WASTEFULL
Low (0V) when the waste recipient is full.
TANKEMPTY
High (5V) when the container is empty.
O.K.TEMP
It is low and then the related LED is off, when the support for plate 1 has reached the
set temperature.
NEEDLE_HOME
High (5V) when the needle is high (HOME).
Includes the 6522 (U12) by which the microprocessor manages the following logics:
Outgoing:
CLOCK091, TX091
Outputs related to motor 1 board (X).
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BRIO 2 Automatic Preparer - Service Manual
CLOCK092, TX092
Outputs related to motor 2 board (Y).
LIFT
If low (0V), enables the motor of the manifold latches.
NEEDLE PUMP
If low (0V), enables the motor of the peristaltic pump washing needle.
VALVEN.O.1
If high (5V), closes the N.O. valve of the 2500 µL syringe.
VALVEN.C.1
If high (5V), opens N.C. valve of the 2500 µL syringe.
VALVEN.O.2
If high (5V) closes the N.O. valve of the 1000 µL syringe.
VALVEN.C.2
If high (5V) opens the N.C. valve of the 1000 µL syringe.
RESET05
If low (0V) resets the two motor boards.
8NEEDLES_PUMP
If low (0V), enables the peristaltic pump for washing the microplates.
Incoming:
TX7051, CLOCK7051
Inputs related to motor 1 board (X).
TX7052, CLOCK7052
Inputs related to motor 2 board (Y).
DATAOUT
Serial for data transmission from serial EEPROM to MC6809.
Includes the 6522 (U15) by which the microprocessor manages the following logics:
Outgoing:
COMMIXER2
If high (5V), enables the shaking of plate 2.
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BRIO 2 Automatic Preparer - Service Manual
ONTERM1
If low (0V), enables the heating of plate 1.
ONTERM2
If low (0V), enables the heating of plate 2.
CS\AD
Selects A/D converter.
A0÷A2AD
Selects NCT binary to be measured.
PLI.LIFT_UP
If high (5V), enables the lifting of the pliers.
CLOSE_PLIERS
If high (5V), enables the closure of the pliers.
Incoming:
PLIERS_LIFT_DOWN_SENS
High (5V) when the pliers are in low position.
PLIERS_LIFT_UP_SENS
High (5V) when the pliers are in high position.
PLIERS_ON_PLATE_SENS
High (5V) when the pliers are being held on the plate.
PLIERS_HOME_SENS
High (5V) when the pliers are in the HOME position.
CPUPOWER
This is the block of the power part with speed control circuits of the peristaltic pump
motor 8 needles (U5C), the plate 1 shaker motor (U5D) and the motor of the 8 needles
latches (U5B), respectively using the trimmers R37, R47 and R28.
CPUAD
This is the block that performs the Analogue/Digital conversion of temperature signals.
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1.10.5. MOTHERBOARD
This is the interconnecting board of the power supply, motor control, processing and
continual motor control boards. It also connects these boards to the connectors, which
reach the peripheries to be controlled using the general wiring.
A EEPROM is present in the board where the calibrating data for every single instrument
is saved.
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This board has the role of interfacing the general wiring with the needle sensor board.
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The circuit is able to measure abrupt variations in capacity such as what is generated
when the needle comes in contact with a conduction liquid.
The gauge’s circuit consists of a frequency/voltage converter, a derivative filter, a fixed
threshold comparator and an analogue monostable.
A second completely analogue section has also been created on the same board to
measure the detachment of the needle from the conduction liquid.
The operation of the circuit is also indicated by a red LED (D4) for the contact and a green
LED (D3) for the release.
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The control circuits of the shaker motor of plate 2 are assembled on this board using the
trimmer R3.
The two relays (K1 and K2) are also present, which are enabled by U5 to supply the
heating resistance of the two plates.
The drivers (U6 and U3) used to move the pliers are also found on the same board.
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This board has the role of interfacing the pliers wiring with the wiring coming from the
needle sensor interface board (and pliers).
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All the power supply circuits are on this board, which supply the following voltages:
b) +5V regulated
The alternating voltage present on feet 9 and 10 of J1 of the nominal value of 25V is
rectified by B1. There is a continual non-stabilised voltage of over 30V at this output.
This voltage is applied at the input of the switching regulator A1 and supplies the regulated
voltage of +5V at the outlet. This voltage can be measured at TP2.
The voltage +5V supplies all the logic circuits of the equipment.
The passive parts R1, C3, D1, L1 and C4 complete the regulator circuit +5V.
c) +24V
The same voltage not regulated by bridge B1 at the outlet using the regulator circuit A4
supplies the voltage stabilized at +24V for the printer (non used). The voltage is instead
used to supply the cooling fans of the preparator rack board.
Together with the circuit A4, there are also passive parts C1, C2, C12, C13, R2, R3, C14
and C15 which complete the +24V regulation circuit.
The voltage +24V can be measured at TP6.
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BRIO 2 Automatic Preparer - Service Manual
8 photometers from D1 to D8 are assembled on this board, as well as four dual amplifiers
U2, U3, U4 and U5. Each of the amplifiers are connected to make them work like
current/voltage converters.
The 8 outputs of the amplifiers are connected to the entry of the analogue switch U1. The
wires coming from pins 25, 27, 29 of J1 are connected to the feet 1, 16, 15. The wires
each come directly from the CPU board. The digital command to close the analogue
switch U1 is present on these wires.
The output of the analogue switch is connected to the impedence adaptor U8B and from
this to the operational amplifier U8A, connected as an amplifier.
R11 and R12 have the job of supplying some offset mV, which are applied at the non-
inverting entry of the amplifier U8A.
The output of the latter is then connected to the entry of the digital analogue converter U7.
The outputs of the latter are connected to the feet from 1 to 12 of J1 and from this finally
connected to the CPU board for the subsequent processing of the photometric signal.
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This board is managed by the microcontroller IC1 (68HC11) with 8MHz clock.
IC22 generates the correct RESET signal.
By jumping J33, the board can be reset without turning off the apparatus.
Jumper 2 Selects operation in a simplified mode, that is to say, without the display,
keyboard and printer (not present). All the output functions are suppressed in this
condition.
Jumper 3 Sets the reader to automatically perform a series of tests related to the
operation of the I/O devices (not present). Do not use this position.
Jumper 4 Sets the configuration to the default values (COP, ROM, EEPROM disabled).
Jumper 5 Selects the operation of the microprocessor in Mode A. Do not use this
position.
On the Bus I/O circuits I/O IC12, IC13, IC14, IC15 and IC16 (68B21) are connected, which
are dedicated to the following functions:
IC12
Keyboard interface (not used) connected to J39 and management of the switches U1A,
U1B and U1C, which allow the regulation of the voltages of the photometric lamp (pin 6 of
J40) to obtain the optimal level of photometric signals with the resistive networks R25 and
R26.
IC13
Printer interface (not used) connected to J38.
IC14
Display interface (not used) connected to J37. The contrast can be regulated with R24.
IC15
Stepper motor interface with outputs from PA0 to PA7 connected to the power drivers IC18
and IC19 (L293), whose outputs are connected directly to the motors through the
connectors J35 (filter motor) and J34 (plate motor).
Analogue interface board to direct the analogue multiplexer with the wires PB4/PB7.
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IC16
Interface with the analogue board to acquire incoming data from the AD converter and 12
Bit on pins 1 to 12 of connector J36 (J1 of the analogue board) and for the commands to
the converter (wires 13 and 23 of J36).
The RAM memories IC5 and IC6 are also connected on the microprocessor bus with an
incorporated battery and EPROM IC4 (27C512) and IC3(27C010).
The Home Position sensors of the filter and plate motors on the connectors J43 and J44
are connected directly to IC1 by the two adaptors IC20A and IC20B.
Buzzer:
is controlled directly by IC1 through T1.
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The schemes related to the general wiring of the photometer/PC assembly and Preparator
assembly of the instrument are included on the following pages.
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1.11.1. USE
The Brio 2 is intended for In Vitro Diagnostic use in laboratories performing clinical assays
on patient samples in microplates. It may also be used in other laboratories requiring the
automation of tests which are carried out in the general EIA format in microplates. The
instrument can be used only by qualified and trained personnel.
The instrument must be used in conjunction with the kits for diagnostic in vitro operations.
For all clinical applications it is necessary to refer to the relative instructions for use. The
user shall be responsible for ensuring that these kits are certified for use in conjunction
with the instrument.
For all information regarding disposal of the waste material together with the instrument
please refer to the instructions for use of the kit.
1.11.2. PERFORMANCE
The Brio 2 is an open system which can be programmed to carry out a wide range of
biological tests. The performance characteristics of the system will vary widely depending
on the parameters set by the user. They MUST be verified in cases where new assays
have been implemented. It is the responsibility of the user to ensure compliance with
the requirements of each individual assay manufacturer.
1.11.3. PROTECTION
The instrument is designed for maximum protection of the user during normal operation.
The sampling arm of the device however, is controlled by the instrument program and may
move suddenly and unexpectedly during a run, having the potential to cause physical
injury. Always keep the protective cover closed when in operation and never reach
into the work area when a run is in progress without first pausing the program or
turning off the system.
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BRIO 2 Automatic Preparer - Service Manual
2.1. FOREWORD
The following paragraphs explain the procedure to follow for correct installation of the
BRIO 2 Robotic immunoassay operator. This information regards:
– instructions for transportation, storing and unpacking of the instrument;
– procedure and particular requirements for the installation (interfacing with other
instruments, controls etc.);
– testing procedure that verifies if the instrument has been correctly installed and if it is
able to carry out its function.
There are certain precautions to keep in mind during transportation, storage and
unpacking of the instrument in order to avoid damages also due to storage in an
unsuitable environment.
TRANSPORTATION
STORAGE
The instrument must be stored in its original packing composed of wooden crates. The
packing must be stored taking heed of the TOP-BOTTOM signs written on the outside.
When placing cases in the storage room leave necessary space around for easy handling,
removal and inspection.
Should the crates be damaged during transportation or storage the instrument must be
inspected to check that no evident damage has occurred; a new integral packaging must
be provided before storage.
If any damage is found, please contact the Manufacturer.
The type of packaging guarantees reliable protection and insulation as long as the crates
are stores in a suitable environment; the storage room must be dry and free from dust.
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BRIO 2 Automatic Preparer - Service Manual
UNPACKING
Due to the weight of the instrument several operators will be required to carry out the
following procedure:
− Carefully remove the packaging from the instrument (keep the packaging in case the
instrument needs to be returned).
− After removing the lid and side strip, remove the 4 anchoring bolts located on the sides
of the instrument.
− Remove the anchoring brackets after unscrewing the 8 anchoring screws on the base
plate.
− Insert the 4 handles supplied in the same holes used for anchoring the instrument.
− Use the aforementioned handles to position the instrument on top of the work surface.
− Remove the handles and make sure to keep them for any future handling operations.
Q.ty Description
1 Power Supply cable
1 Kit mouse and keyboard
1 Multiple anti-disturbance socket
1 Triple socket
1 Sampling needle
2 Sera rack
1 C Reagent Rack
1 D Reagent Rack
1 Compressor
1 Waste tank
1 Internal needle washing solution tank (10 liters)
1 External needle washing solution tank (5 liters)
3 Peristaltic pump tube
2 Washing tubes
1 Set of internal washing solution caps
1 Set of external washing solution caps
1 Set of waste tank caps
1 Level adaptor cable
1 L shaped spanner
1 Kit of instrumentation accessories
1 Kit of basic instrumentation (containing TRITON X100)
1 Software CD
1 User’s Manual
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BRIO 2 Automatic Preparer - Service Manual
The operative requirements are very important with regard to the selection of the room
where the installation is to be carried out.
The Robotic immunoassay operator must never in any case be installed in dusty sites or
where there are corrosive fumes.
The overall dimensions of the Robotic immunoassay operator are 44 x 111 x 60 cm
however it is recommended leaving sufficient space all around in order to facilitate
maintenance operations.
Leave at least 10 cm on the back, it allows all the electric connections, the right position for
the tubes (straight) and a good heat dissipation.
The primary points necessary for installing the instrument are as follows:
- Identification of a person to be in charge of the installation procedures who will get in
touch with the Manufacturer for all clarifications.
- Selection of the environment where the installation is to take place.
- Definition of the best layout of the system in the installation site.
- Purchase of any additional accessories apart from those supplied.
Although the Robotic immunoassay operator has been designed with components capable
of operating in unfavorable operating conditions, a thorough check of the environment is
recommended in order to ensure the most reliable performance.
- High temperatures will accelerate the aging process of the components of the
Robotic immunoassay operator, causing temporary and even permanent
transformation in these components.
- Vibrations may cause wear and tear of the mechanical parts of the system.
- High frequency and high intensity pulses generated in the electric lines or induced
by the surrounding environment may cause errors in the system.
- Do not position the instrument near any heat sources like radiators, hot air tubing, or
places subjected to direct sunlight.
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BRIO 2 Automatic Preparer - Service Manual
- Install the instrument on a flat surface, not inflammable; the surface must have at
least the same dimension of the instrument.
A cable with three wires and a plug with 3 contacts is supplied for each instrument for
connection to the power supply and grounding. Connect the instrument to a plug with the
protection earth contact, controlled by a differential switch.
Grounding that does not comply with standards may be a hazard for the users and cause
malfunction of the instrument.
Ensure that the power supply outlet in the room where the device is to be installed
complies with the one specified and that a good earth connection is guaranteed. At the
time of positioning the BRIO 2 make sure that the work surface is completely level and that
it is not close to heat sources or walls that could prevent correct aeration and it’s also
preferable to place the instrument far from other instruments in order to avoid mutual
interactions.
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BRIO 2 Automatic Preparer - Service Manual
Use the relative L-shaped spanner supplied with the instrument to remove the lower screw
and open the front panel where the PC is positioned. Make the panel to slide up and to
have the panel out of its upper housings: In this way you obtain the panel to be removed.
Connect the keyboard and mouse “V” cable to the appropriate connector and connect the
repeater of the wire less system to this cable having it to pass firstly through the opening
positioned on the back of the instrument. At the same way, when needed, the cable for the
printer and for the LAN connection should be connected having the cables to pass through
the back opening. Re-assemble the front panel by inserting the two hooks in the
appropriate housings by sliding down the panel. Fix the panel with the low screw. Put the
keyboard an the mouse on the same table where the BRIO 2 is positioned
Place all the tanks on the same bench as the BRIO 2 and put the waste tank and the
compressor on the floor.
The external needle washing solution ( 5 liter tank) must be prepared with deionized water
and 25 mL of Triton X200. (VSM3 Basic Kit).
The internal needle washing solution (10 liter tank) must be prepared with deionized water
and 50 mL of Triton X200. (VSM3 Basic Kit)
In some cases and when indicated by specialists of the product, this solution may be of a
different nature.
Connect the tubes and the level sensors of the tanks marked A-B-C-D-E*-F*-1-2 to their
matching connectors in the rear section of the instrument as shown on the nameplate
attached to the rear panel of the BRIO 2 (Fig. 2.1).
Check that the tubes are not squashed or twisted preventing a normal circulation of the
fluids.
Insert the power supply cable of the compressor into the specific socket of the BRIO 2.
Insert the power supply cables of the BRIO 2 and the PC into the anti-spike multiple-
socket (supplied with the instrument) and turn on in sequence the BRIO 2 - Personal
Computer – Drive.
Warning: It is recommended never to position the compressor on the same work bench
as the "system", in order to prevent the vibrations from compromising the
correct functioning of the devices.
Warning: The 10-liter tank and the buffer tank must be positioned on the same level as
the BRIO 2, in order not to compromise the precision of the dispensations.
Warning: for any movements carried out at high acceleration the instrument only works
with the front panel closed.
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BRIO 2 Automatic Preparer - Service Manual
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BRIO 2 Automatic Preparer - Service Manual
During testing to be carried out with the user, the technician must:
– Verify the self test procedure execution
– Explain the features of the Analyzer.
– Explain the function of each device.
– Explain the various operating procedures. Make the user repeat these operations so
that he understands their function.
– Make sure the user fully understands the features of the Analyzer and is perfectly
capable of using it correctly. Explain the maintenance procedure.
2.6. DISINSTALLATION
Insert all the tank cups in a distilled water tank; disconnect the level sensor; run at list
three wash and preparatory priming. Empty completely the waste tank using the pump
and, if necessary, manually (use protective mask and gloves).
Disconnect the hydraulic and electric connection on the rear panel (tanks, compressor,
power cable, host connection).
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BRIO 2 Automatic Preparer - Service Manual
3. MAINTENANCE
– Keep the external surfaces of instrument clean using a soft cloth dampened with a
warm, mild detergent solution.
– Check the sample needle: for the best results it is recommended to wipe the tip with a
swab soaked in alcohol to prevent any build-up of protein.
– Clean up any accidental spills in the work area: if hazardous biochemical has been
spilt it is recommended cleaning a sterilizing agent.
– Check the FLUSHING level and if necessary top up. It must be remembered that the
solution consists of a dilution of one 50 mL bottle of Tritone solution for every 10 liters
of distilled or deionized water.
- Carry out the washing procedure by following the instructions in the window displayed
by the program in order to clean the needle and manifold.
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BRIO 2 Automatic Preparer - Service Manual
Prepare the flushing solution by adding 50 ml of triton solution to every 10 liters of distilled
or deionized water. Top up the tank with the specific liquid.
Wash the sampling needle and the manifold by following the WASHING procedure in the
CONFIGURATION /TOOLS submenus.
N.B.: If the instrument is not used on a daily basis it will be necessary to carry out the
priming operation every week in order to prevent the tubing from closing inside the
N.C. valves.
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BRIO 2 Automatic Preparer - Service Manual
Depending on the number displayed by the counter located above the mother
board, the instrument could require preventive maintenance as indicated in the
following chart.
50,000 9 9 9
100,000 9 9 9 9 9
150,000 9 9 9
200,000 9 9 9 9 9
250,000 9 9 9
300,000 9 9 9 9 9
350,000 9 9 9
400,000 9 9 9 9 9
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BRIO 2 Automatic Preparer - Service Manual
Any blocks in the drainage well should be removed by using a piece of fine steel wire
introduced into the hole at the bottom of the well itself.
Before carrying out the following procedures switch off the power and disconnect
the plug.
In order to replace the piston refer to Fig. 3.1. In this figure the various elements making
up the Dilutor are indicated separately.
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BRIO 2 Automatic Preparer - Service Manual
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BRIO 2 Automatic Preparer - Service Manual
Before carrying out the following procedures switch off the power and disconnect
the plug.
In order to replace the tubing refer to Fig. 3.1 that illustrates the details of the Dilutor Unit.
N.B.: Warning during this phase the syringe will remain hanging from the small tubing
only (6) and (7).
- Remove one of the tubing (6) and (7) from one end of the tubing-union end (9) and
the other from the other end of the tubing union (10).
- Prepare the new tubing.
- Insert the new tubing into the tubing union (9) (10).
- insert the tubing (6) and (7) into the grate of the pinch valve (11).
- Reinsert the syringe unit (4) into its housing.
- Restore the position of the piston by inserting it into its hole in the pin (3).
- Reinsert syringe locking knob (5) and screw it up.
- Reinsert the locking knob (1) and screw it up.
N.B.: The same steps can be followed for replacing tubing (8) and (12).
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BRIO 2 Automatic Preparer - Service Manual
Before carrying out the following procedures switch off the power and disconnect
the plug.
In order to carry out the replacement of the needle please refer to Fig. 3.2 and Fig. 3.3.
- Turn off the instrument.
- Lift up the transparent cover.
- Extract the Sample and Reagents stands from their housings.
- Manually shift the mobile arm in line with the sample housing.
- Unscrew the hydraulic union of the needle.
- Disconnect the gudgeon (electrical connection of the level sensor).
- Unlock the needle from its seat by rotating it as indicated in Fig. 3.2 points B & C.
- Pull the needle out towards the top as indicated in Fig. 3.3.
- Prepare the new needle.
- Insert the new needle in the housing and repeat the same steps in the reverse.
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BRIO 2 Automatic Preparer - Service Manual
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BRIO 2 Automatic Preparer - Service Manual
Some simple controls and cleaning and maintenance procedures help to keep the reader
in good operating conditions. In particular, the following is recommended:
- Check the powering up time of the lamp to replace them in a timely manner.
- Check the cleaning of the accessible internal parts, making absolutely sure that
water, alcohol and/or similar are not used. Use a soft, dry cloth for any operations.
Before performing this procedure, turn off the instrument using the switch and disconnect it
from the main by removing the plug from the mains socket.
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BRIO 2 Automatic Preparer - Service Manual
Before performing this procedure, turn off the instrument using the switch and disconnect it
from the main by removing the plug from the mains socket.
- To access the photometer bay, unscrew the anchoring screw of the inclined front
panel and replace it after having unlocked it, making it slide upwards.
- Unscrew the two screws which fix the Photometer Electronic Carter, the four screws
which fix the Photometer Carter and remove it.
- Unscrew the screws which lock the filter to be replaced.
- Remove the filter and insert the new one.
- Tighten up the two screws without using undue force.
- Re-position the Photometer Carter, Photometer Electronic Carter and tighten up all
the anchoring screws.
- Re-position the inclined front panel, lean it on the housing and make it slide
upwards and tighten up the anchoring screw.
- Connect the instrument to the mains power supply.
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BRIO 2 Automatic Preparer - Service Manual
To access the SERVICE functions, press the relative button on the main menu.
If the password has been set, the request window appears on the screen, and the window
in Fig. 3.5 if inserted correctly.
Prep
Allows access to the movement and sending of commands to the preparator (Fig. 3.7)
Show Comm
Displays the list and parameters related to commands
Setup
Allows access to the calibrating and checking functions (Fig. 3.26)
The button is disabled if the program was started in Offline mode. Press the Control key on
the keyboard if you want to access the related functions and click on the button while
keeping it pressed down.
Sequences
Performs the completion of the operational sequences
Password
Allowing the SERVICE access password to be changed
Exit
Goes back to the main menu
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BRIO 2 Automatic Preparer - Service Manual
Preparator
Using the window Fig. 3.7, all the movements and activations set out for the preparator
can be performed by sending individual commands or command sequences and
displaying the status of the preparator.
Syringes
Allows the movement of the syringes (Fig. 3.8)
Purge Syrin
Allows the “purge” to be performed (advancement to the mechanical end strike) of the
syringes (Fig. 3.9)
Axis
Allows the movement of the X, Y, Z axes and manifold (Fig. 3.10)
Elet. Valve
Allows the movement of the dilutor’s solenoid valves (Fig. 3.11)
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BRIO 2 Automatic Preparer - Service Manual
Get Ver
Obtains the firmware versions
Perist Needle
Allows the movement of the needle washing peristaltic (Fig. 3.12)
Perist Manif
Allows the movement of the peristaltic of the manifold power supply (Fig. 3.13)
Home
Allows the home procedure to be performed (Fig. 3.14)
Shaker
Allows the shakers to be activated (Fig. 3.15)
Cover Lock
Allows the panel block to be activated (Fig. 3.16)
Thermo
Allows the heaters to be activated (Fig. 3.17)
Beeper
Allows the sound signal to be activated (Fig. 3.18)
Trace / Test P
Allows the values to be set to control the execution (Fig. 3.19)
Dilutor Init
Perform the initiation procedure of the preparator
Wait
Allows a wait command to be sent (Fig. 3.20)
Stop Shaker
Allows the shakers to stop/start (Fig. 3.21)
Pliers
Allows the movement of the pliers (Fig. 3.22)
Output
Allow the movement of the solenoid valves (Fig. 3.23)
Exit
Goes back to the previous window
Send
Sends the commands contained in the top window of the preparator
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BRIO 2 Automatic Preparer - Service Manual
Clear
Clears the content in the top window
Save
Saves the content of the top window in a file
Load
Loads the commands contained in a file in the top window
Editing
Allows the editing command functions to be set in the top window. When the function is
active, the commands obtained are not sent to the preparator, but are written in the top
window. The GO button in the windows described as follows assumes the Create function.
On
Enables the function
Off
Disables the function
Get State
Allows the viewing of the details of the state of the preparator (Fig. 3.24)
Short State
Displays the short state of the preparator in the top window
Reset µP
Performs the resetting of the microprocessor
Get Temp
Visualises the temperatures of the plates in the top window
Halt
Immediately halts the execution of the sequence of commands in the buffer
Start
Starts the execution of the sequence of commands in the buffer
Stop
Stops the execution of the sequence of commands in the buffer, in correspondence to the
first test point
Switch Buffer
Allows the buffer to be switched for the commands (Fig. 3.25)
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BRIO 2 Automatic Preparer - Service Manual
Reset
Resets any errors
Test
Performs the commands in the buffer up to the first test point
Step
Performs the first of the commands in the buffer
Syringes
Syringes
1, 2, 1-2
Allows the movement of the syringes to be selected
Speed
0 (slow), 1, 2, 3 (fast)
Allows the movement speed to be selected for each syringe
Step
Full Step, Half Step
Allows the movement mode to be selected for each syringe
Absolute position
Allows the final position of the movement to be set for each syringe
GO
Performs the movements with the set parameters
Exit
Goes back to the previous window
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BRIO 2 Automatic Preparer - Service Manual
Purge Syrin
GO
Purges the selected syringes
Exit
Goes back to the previous window
Axis
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BRIO 2 Automatic Preparer - Service Manual
The following parameters can be set for each of the movements X, Y, Z and Z-Sens
(needle with active level sensor):
Speed
0 (slow), 1, 2, 3 (fast)
Allows the movement speed to be selected from 0 (slow) to 3 (fast)
Step
Full Step, Half Step
Allows the movement mode to be selected from full step and half step
It is possible to choose between the following for the movement of the manifold:
Up
Takes the manifold into the up position
Down
Takes the manifold into the down position
Absolute Position
Final position of the movement (in steps)
Axis
X, Y, Z, XY, Manifold, Z-Sens
Allows the selection of the movement to be executed
GO
Performs the selected movement with the parameters set
Exit
Goes back to the previous window
Elet. Valve
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BRIO 2 Automatic Preparer - Service Manual
The following parameters can be set for each of the dilutor’s solenoid valves:
Activate
Movement of the relative valve or not
On
Enables the valve
Off
Disables the valve
GO
Executes the selected movements
Exit
Goes back to the previous window
Perist Needle
Time
Duration of movement in 1/20 sec.
GO
Performs the movement with the set parameter
Exit
Goes back to the previous window
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BRIO 2 Automatic Preparer - Service Manual
Perist Manif
Time
Duration of movement in 1/20 sec.
Direction
Direction of rotation of the peristaltic (Forward and Reverse)
GO
Performs the movement with the set parameter
Exit
Goes back to the previous window
Home
GO
Performs the selected home
Exit
Goes back to the previous window
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BRIO 2 Automatic Preparer - Service Manual
Shaker
Time
Duration of movement in 1/20 sec.
Speed
Shaking speed (from 0 to 15)
GO
Enables the shaking selected with the parameters set
Exit
Goes back to the previous window
Cover Lock
Close
Closes the cover
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BRIO 2 Automatic Preparer - Service Manual
Open
Disables the closure of the cover
GO
Performs the selected operation
Exit
Goes back to the previous window
Thermo
Plate 1, 2, 3, 4
Plate to be heated (*disabled on the monoplate model)
Time
Heating duration in 1/20 sec.
Speed
Code of the heating temperature (Temp./2 - 7)
GO
Enables the thermostat selected with the parameters set
Exit
Goes back to the previous window
Beeper
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BRIO 2 Automatic Preparer - Service Manual
Sets the activation mask of the beeper by ticking the desired boxes.
GO
Performs the beeper with the selected mask
Exit
Goes back to the previous window
Trace / Test P
Value
Value to be set as trace / test point.
GO
Performs the selected set-up with the set parameter
Exit
Goes back to the previous window
Wait
Time
Wait time in 1/20 sec.
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BRIO 2 Automatic Preparer - Service Manual
GO
Sends a wait command with the set parameter
Exit
Goes back to the previous window
Stop Shaker
Stop, Start
Type of command to be applied
GO
Performs the selected operation
Exit
Goes back to the previous window
Pliers
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BRIO 2 Automatic Preparer - Service Manual
Close / Open
Performs the closure/opening movement of the pliers
Down / Up
Performs the lowering/lifting of the pliers
GO
Performs the selected operation
Exit
Goes back to the previous window
Output
GO
Performs the selected operations
Exit
Goes back to the previous window
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BRIO 2 Automatic Preparer - Service Manual
Dilutor State
Sensors
Axis, Syringes, Washing Tanks, Manifold, Liquid level, Thermostat, Cover
Shows the status and/or any errors with the related device
Task
Serial Port
Shows the status of the serial communication and the command buffers
Task On Run
Shows the status of active processes
Last Trace/Test P
Shows the control parameter to be used
FW Version
Shows the firmware versions
Get State
Updates the state
Ok
Goes back to the previous window
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BRIO 2 Automatic Preparer - Service Manual
Switch Buffer
Buffer
Buffer to be activated (0, 1, 2)
GO
Performs the operation with the set parameter
Exit
Goes back to the previous window
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Setup
Calibrations and checks on the preparator’s devices can be performed using the window in
Fig. 3.26, as well as sending immediate commands and showing the EEPROM content.
Movements
X Axis, Y Axis, Syringe 1, Syringe 2, Needle, Manifold, Pliers
Determines the type of movement
Steps
Sets the number of steps to be performed for the subsequent movement commands
Test
Coordinate
Allows the calibrating to be performed for the reference positions (Fig. 3.27)
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BRIO 2 Automatic Preparer - Service Manual
Immediate Command
Allows an immediate command to be sent to the preparator (Fig. 3.28)
K Factor
Allows the movement reports to be shown and changed (Fig. 3.29)
Procedures
Allows a series of test procedures to be performed (Fig. 3.30)
Eeprom
Allows the content of EEPROM to be shown
Positions step / mm
X Axis, Y Axis, Needle, Syringe 1, Syringe 2
Shows the current position of the needle / manifold unit and needle in steps/mm.
Manifold
Shows the current position of the manifold.
Exit
Goes back to the previous window
Coordinate
The calibrations of the preparator’s devices can be performed and checked using the
window in Fig. 3.27.
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BRIO 2 Automatic Preparer - Service Manual
Test
Reagent, Samples, Parking, Needle Wash, Manifold Wash, Photo
Takes the needle / manifold / pliers unit into the saved position in relation to the button
pressed
Plates
Needle
Takes the needle into the saved position in relation to the selected plate
Manifold
Takes the manifold into the saved position in relation to the selected plate
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BRIO 2 Automatic Preparer - Service Manual
Pliers
Takes the pliers into the saved position in relation to the selected plate
1, 2, 3, 4
Takes the needle, manifold or pliers into the saved position for the selected plate in
related to what has been selected
Home
Performs the home of all the preparator’s devices
Movements
Needle / Manifold / Pliers
Up / Down
Moves the needle up and down for the steps set in Steps in relation to the type of test
being executed or the manifold or pliers in the up / down position
Pliers delay
Sets the delay to determine the run number of the pliers
Z max.
Mem
Saves the current position of the needle as position Z max
Up
Takes the needle into the position saved as Z max
Down
Takes the needle into home from the position saved as Z max
X/Y
Up, Down, Left, Right
Moves the needle / manifold / pliers unit of the number of steps set in Steps in the
direction related to the button pressed
Zero
Moves the needle / manifold / pliers unit into the position saved as zero
Save
Saves the current position in relation to the type of test being executed.
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Positions
X Axis, Y Axis, Needle
Shows the current position of the needle / manifold and needle unit in steps/mm
Manifold
Shows the current position of the manifold
Pliers Lift
Shows the current position of the pliers
Zero Mem.
Saves the current position of the needle / manifold unit as Zero
Read Eeprom
Reads the parameters saved in EEPROM
Write Eeprom
Writes the current parameters in EEPROM
Save Ee In File
Saves the calibrating parameters in a file
Exit
Goes back to the previous window
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Immediate Command
Home
Performs the home of the preparator devices
Get Ver
Obtains the firmware versions
GetState
Obtains the state of the preparator
Reset uP
Resets the microprocessor
Cover Lock
Activates the cover lock
Halt
Immediately halts the command sequence in the buffer
Start
Starts the command sequence in the buffer
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Stop
Stops the execution of the command sequence in the buffer, in correspondence with the
first test point
Switch Buffer
Allows the switching of the buffer for the commands
Reset
Resets any errors
Exit
Goes back to the previous window
K Factors
X Axis
Conversion ratio value mm / steps
Y Axis
Conversion ratio value mm / steps
Needle
Conversion ratio value mm / steps
Syringe 1
Conversion ratio value µL / steps
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BRIO 2 Automatic Preparer - Service Manual
Syringe 2
Conversion ratio value µL / steps
Exit
Goes back to the previous window
Save
Saves the values in a file
Procedures
Some test procedures for the preparator devices can be performed from the window in Fig.
3.30.
Syringes
Performs the syringes movement test
Needle
Performs the needle movement test
Axis
Performs the movement test of the X and Y axes
Manifold
Performs the manifold movement test
All
Performs the activation test of the solenoid valves and heaters
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Level Sensor
Allows the level sensor test to be accessed
Wells
Allows the positioning test of the wells to be accessed
Pliers
Performs the movement test of the pliers
Run
Runs the selected option
Stop
Stops the ongoing test
Home
Performs the home of the preparator devices
Exit
Goes back to the previous window
Level Sensor
Layout
Serum
Name of the active serums layout
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Reagent
Name of the active reagents layout
Test Position
Serum
Performs the test up in a serums layout position
Reagent
Performs the test up in a reagents layout position
Number
Position in which the test is performed
X
X coordinate of the selected position
Y
Y coordinate of the selected position
Z max
maximum Z of the selected position
Mode
Speed
1, 2, 3, 4
speed selected for the run of the needle
Step
1, ½
mode selected for the run of the needle
Continuous
If marked, performs the test in all the positions after the one selected
Run Number
Run number to be performed in every position
Current Run
Number of current run
Steps
Steps resulting in the current run
View
Shows the test log
Go
Performs the test with the selected parameters
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Stop
Stops the ongoing test
Exit
Goes back to the previous window
Wells
Device
Needle
Performs the needle positioning test
Manifold
Performs the manifold positioning test
Mode
Continuous
Continually performs the test
Step
Performs the test, stopping after every position
Plate
1, 2, 3, 4
The plate on which the test is performed
Start
Starts the test
Stop
Stops the test
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Repeat
Repeats the test in the last tested position
Restart
Restarts the interrupted test
Home
Performs the home of the preparator devices
Exit
Goes back to the previous window
The instrument fails to The front cover is not Close cover to trigger sensors.
move the arm. closed (safety interlock
engaged)
No display appears on Power supply cable not Connect the power supply cable
turning on PC (mod. S) connected. or the video cable at the back of
the PC.
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BRIO 2 Automatic Preparer - Service Manual
The washing liquid floods Waste tank not fitted into Reconnect the waste tank.
out of the washing well place correctly.
manifold or the strip Replace the cap of the waste
during their washing tank or the waste tubing
operations, or the connector.
Flushing Solution floods
out of the needle washing If flooding of the waste occurs
chamber. due to the waste tubing
breaking, turn off the machine,
unload the plates and clean up
the excess fluids.
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BRIO 2 Automatic Preparer - Service Manual
The needle hits against Layout selection incorrect. Ensure that the reagent plate
something. matches the diagram selected in
the layout of the EIA module.
The caps have remained Remove all the caps from the
in the vials of reagents. reagent vials to use before
positioning the vials on the
layout.
The strips are incorrectly Ensure that all the strips are
positioned on the plates correctly positioned and that the
or the plates are badly plates have been properly
positioned on the inserted into the instrument.
instrument .
The mobile arm does not Clean the axles.
work smoothly
Call the Technical Service
Center.
The needle loses liquid There’s not liquid in the Fill the solution-filling bottle.
and does not distribute solution-filling bottle (10-
sufficient liquid. liter tank).
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BRIO 2 Automatic Preparer - Service Manual
Error in reading the plates. The reader lamp has burnt Replace the reading
out. lamp.
Liquid errors detected in vials The level of the liquid is too Do not overfill the vials.
which are full. high for the vials. The
liquid/air limit has not been
detected.
Plates not sufficiently washed. Peristaltic pump tube worn Replace the tube.
out.
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BRIO 2 Automatic Preparer - Service Manual
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BRIO 2 Automatic Preparer - Service Manual
The printer fails to respond. The printer is not Check the power supply
connected correctly. cable of the printer.
The printer alarm light is on. The paper is either missing Reload the paper
or blocked in the printer.
Remove any pieces of
paper blocking the printer.
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BRIO 2 Automatic Preparer - Service Manual
4. APPENDIX
The current management of the liquid detection in the vials carried out a control three
times in the case of absence of liquids as the needle descends. On the third control the
error will be signaled by means of an acoustic alarm.
The user can in any case still carry on with the readings as these will remain visible. On
carrying out the visualization of the results, any error due to the lack of liquid in a vial will
be evidenced.
In the average of the OD, concentrations and results these errors are not taken into
account in order to ensure a correct assessment.
Before filing the results in the historic archive, the user can in any case re-enable any of
the wells with errors.
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BRIO 2 Automatic Preparer - Service Manual
The entries ‘id’, ‘Ins_Day’, ‘Ins_Month’, ‘Ins_Year’ and ‘Test’ are always compulsory whilst
the ‘Result’ entry is compulsory (and permitted) only towards the host.
If several tests are required for a patient, they must be entered under ‘Test’ on the same
line, and the entries must be separated from one another by commas.
The results are indicated in the same order as under the ‘Test’ entry. They are separated
by commas and contain the same notes as those used in the medical reports.
For allergologic tests, a test must be indicated for each allergen to be dispensed, and
combine the test code of the allergologic type with the allergen code, and contain the
notes (following is an example of the code number of the test of the allergologic type on
the assumption of dosing the allergens d1, d2, f5, f221, w19 to the patient together with
any other tests):
Test = […..,]n/d1,n/d2,n/f5,n/f221,n/w19[,…..]
For the Avidity type tests with repeats towards the host, a test will appear following by a
result for every repeat (following is an example of the code number of the Avidity type test
with three repeats):
Test = […..,]n,n/Rep2,n/ Rep3[,…..]
RESULTS FORMAT
QUANTITATIVE TESTS
The result is expressed in the form of a seven-figure decimal number (including the point
and two decimal places).
If the value of the concentration is outside the curve, the result is expressed as < or > of
the extremes of the curve (as a concentration).
If the result cannot be expressed, it is marked ‘NV’.
For the allergologic tests the concentration results are followed by the class consisting of
three characters (c ?, c>?, c<?)
(Example: 123.45, 123.45 c 5, 2.87, 2.87 c 2, > 240.00, > 25.00 c>3, < 10.00, < 1.10 c<2,
NV)
QUALITATIVE TESTS
The result is expressed in the form of an index (or ratio) followed by ‘POS’, ‘Dub’ or ‘neg’.
The index is expressed in the form of a seven-figure decimal number (including the point
and two decimal places).
If the OD value cannot be measured (overflow), the index is expressed as > of the index
value in relation the maximum measured OD.
If the result cannot be expressed, it is marked ‘NV’.
For the Avidity-type tests the result is expressed in the form of the avidity percentage
followed by LA, MA, HA or in the form of ND.
(Example: 1.25 POS, 0.66 neg, 1.01 Dub, > 40.38 POS, NV, 13.45 LA, 40.67 MA, 67.33
HA, ND)
N.B.: The examples indicated above represent the positions relating to the different
result components and not of the absolute positions, seeing that these can be
preceded, followed or interspersed by a varying number of spaces (ASCII 32).
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BRIO 2 Automatic Preparer - Service Manual
ACK - Acknowledgement (06H). The receiver sends this character after every block
that has been received correctly.
NAK - Negative Acknowledgement (15H). the receiver sends this character after every
block that is incorrectly received.
EOT - End of Transmission (04H). This character informs the receiver that the last
block has been sent and that the transmission is finished.
SENDER RECEIVER
<----- <NAK>
<SOH> 01 FE <128 data bytes > <checksum> ----->
<----- <ACK>
<SOH> 02 FD <128 data bytes > <checksum> ----->
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