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HPLC Preventative Maintenance Check-list

A preventive maintenance checklist is a set of written tasks that guide the

technician through a preventive maintenance before it can be closed. A checklist
gets all the steps and information out of a manual and into the hands of
experienced technicians.

HPLC Preventative Maintenance Check-list

Customer name: _______________________________________

Address:___________________________________________________________________

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Instrument serial number: ______________________________

Instrument ID: ______________________________
Date: ______________________________

1. Tick (check) each box as the steps are completed.

2. Cross (strike out) each box if the step cannot be completed successfully or a
condition rectified.
3. Mark "NA" for any step that is not applicable.
4. Reset the counter and date for any consumable part that is replaced, by
selecting the desired part replaced action.
5. Run the appropriate Service test for any module undergoing replacements
of major components.

Initial Performance Evaluation

⬜ Conduct a System Test in the software, and make sure that it passes. Print the
results and store a copy on the PC.

Leak Sensor on the Leak Tray

⬜ Enable the leak sensor on the leak tray by checking the "enable" leak sensor
box in the system parameters page. The action should be "stop pumps
immediately".
⬜ Start the Pumps and spill enough water in the area of the leak sensor to see it
pour down the drain tube. Ensure that the pumps go off line.

Analytical Flows Module

⬜ Check the inlet and outlet check valve lifetime counter and change the check
valves if needed.
⬜ Check the number of hours on the Piston Seal in the counter page and replace
if necessary.
⬜ Check the number of hours on the Drain Valve Seal and Drain Valve Filter and
replace if required.
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⬜ Check the number of hours on the Solenoid Valve and replace if needed.

⬜ Check the number of hours on the Mixer filter and Solvent filter and replace if
required.
⬜ Check the number of hours on the 4 way joint and replace if required.

Semi-Prep/Prep Flows Module

⬜ Check the number of hours on the Piston Seal (A, B, and injector pump) in
the counter page for each pump in the system, and replace if necessary.
⬜ Check the inlet and outlet check valve lifetime counter for each pump in
the system (A, B, and injector) and change the check valves if needed.
⬜ Check the number of hours on the 1.2 ml SS mixer filter and replace if
required. Also replace the magnetic stirrer if it looks damaged

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⬜ Check the number of hours on the solvent filter and replace if required.

Autosampler Module

⬜ Check the number of strokes on the syringe and replace if required.

⬜ Check the number of movements since the injector port seal was replaced
and replace if required.
⬜ Check the number of movements for the injection valve stator and
rotor and replace if required.

⬜ Check the number of movements for the syringe valve stator and rotor and
replace if required.

Manual Injector Module

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⬜ Check the number of switches on the column valve rotor and stator and
replace if required.
⬜ Check the number of switches on the column valve stator and replace if
required

Column Heater and Switching Valve Module

⬜ Check the number of switches on the column valve rotor and stator and
replace if required.
⬜ Set the column temperature in the status panel, turn the oven on and read
back the temperature.

PDA/UV-Vis Detector Module

⬜ Check the number of D2 (UV) lamp ignitions and running lamp hours. Replace
the lamp if necessary and run a lamp calibration.
⬜ Check the number of Quartz Halogen (Vis) lamp ignitions and running lamp
hours. Replace the lamp if necessary and run a lamp calibration.
⬜ Check the number of running hours on the flow cell and clean if necessary

⬜ For the UV/Vis detector check the percentage of D2 Lamp intensity loss in the
counter page and replace if required
⬜ Under the service test tab in software run a wave scan test and check to make
sure that the lamp counts for the D2 lamp at maxima are above 2000 counts. If
they are not, then replace the D2 lamp, clean the flow cell and try it again.

RI Detector Module

⬜ Check the number of running hours on the LED and replace if required.

⬜ Clean the flow cell. If replacing the flow cell, ensure that both flow cell
seals are also replaced.

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ELSD Module

⬜ Check the number of running hours on the LED and replace if required.

⬜ Check the number of hours run since the last Evaporator Tube cleaning and
clean the evaporator tube if necessary.

FL Module

⬜ Check the number of running hours on the Xenon lamp, and replace the lamp
if required. Remember to run a re-alignment of the lamp if it is replaced.
⬜ Check the number of running hours on the Hg lamp, and replace this lamp if
required. No final alignment is required for this lamp.
⬜ Check the number of running hours since the last flow cell clean and clean
or replace as necessary.

Scale Up Module

⬜ Check the number of switches on the column valve rotor and stator and
replace if required.
⬜ Check the number of switches on the column valve stator and replace if
required.

Fraction Collector Module

⬜ Check the number of switches on the solenoid valve and replace if necessary.

⬜ Check the number of hours run since the last Evaporator Tube cleaning
and clean the evaporator tube if necessary.

Instrument and environmental conditions

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Good Fair Poor Room temperature:

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Comments and recommendations

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Signatures

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Customer: ____________________ Technician: ____________________

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