Beef Extract
References
1. Baird-Parker. 1962. J. Appl. Bacteriol. 25:12.
2. Baird-Parker. 1962. J. Appl. Bacteriol. 25:441.
3. Baird-Parker. 1963. J. Gen. Microbiol. 30:409.
4. Baird-Parker. 1965. J. Gen. Microbiol. 38:383.
5. Tardio and Baer. 1971. J. Assoc. Off. Anal. Chem. 54:728.
6. Baer. 1971. J. Assoc. Off. Anal. Chem. 54:732.
7. Horwitz (ed.). 2007. Official methods of analysis of AOAC International, 18th ed., online. AOAC
International, Gaithersburg, Md.
Availability
Difco™ Baird-Parker Agar Base
AOAC BAM CCAM COMPF ISO SMD SMWW USDA
Cat. No. 276840 Dehydrated – 500 g
276810 Dehydrated – 2 kg Cat. No. 223950 Prepared Plates (complete) – Pkg. of 10*
Beef Extract Powder • Bacto™ Beef Extract,
Desiccated • Beef Extract
Intended Use
Beef Extract Powder, Bacto™ Beef Extract, Desiccated and Beef
Extract (paste) are used in preparing microbiological culture
media.
Summary and Explanation
Beef Extract is intended to replace aqueous infusion of meat in
microbiological culture media. Beef Extract is frequently used
at a concentration of 0.3 to 1.0% in culture media, although
concentrations may vary depending on the nutritional require-
ments for the medium formulation. Beef Extract may be relied
upon for biochemical studies, particularly fermentation reac-
tions, because of its independence from fermentable substances
that would interfere with the accuracy of such determinations.
Beef Extract was used in media for early studies of nonsporu-
lating anaerobes of the intestinal tract and as a stock broth in
the study of nutritional needs of streptococci. Prokofeva et al.1
used Beef Extract for growing thermoacidophilic organisms
newly isolated from hot springs in Kamchatka, Russia. Kataoka
and Tokiwa2 used Beef Extract as a nitrogen source in studies
of mannose production by Clostridium tertium strains isolated
from soil and methanogenic sludge. In addition, Beef Extract
is a nutritive ingredient in many classical culture media,
including Antibiotic Assay media described in The United States
Pharmacopeia,3 and several media recommended for standard
methods applications.4-6
Beef Extract Powder is a meat extract dried to a powdered
form.
Bacto Beef Extract, Desiccated is the dried form of Beef
Extract (paste) and was developed to provide a product for ease
of use in handling.
Beef Extract is a meat extract in paste form.
These products are to be used in a one for one substitution;
however, variations tend to be formulation-specific and require
actual performance testing.
Difco Manual Sect III Ba.indd 73
Difco™ EY Tellurite Enrichment
AOAC BAM CCAM COMPF ISO SMD SMWW USDA
Cat. No. 277910 Bottle – 6 × 100 mL*
BBL™ Baird-Parker Agar
B
AOAC BAM CCAM COMPF ISO SMD SMWW USDA
United States and Canada
Cat. No. 297214 Prepared Plates (complete) – Pkg. of 20*
297725 Prepared Plates (complete) – Ctn. of 100*
Europe
Cat. No. 255084 Prepared Plates (complete) – Pkg. of 20*
Mexico
*Store at 2-8°C.
Principles of the Procedure
Beef Extract Powder, Bacto Beef Extract, Desiccated and Beef
Extract (paste) are derived from infusion of beef and provide
an undefined source of nutrients. These Beef Extract products
are not exposed to the harsh treatment used for protein
hydrolysis, so they can provide some of the nutrients lost
during peptone manufacture.7 Beef Extract Powder, Bacto Beef
Extract, Desiccated and Beef Extract (paste) are mixtures of
peptides and amino acids, nucleotide fractions, organic acids,
minerals and some vitamins. The function of these Beef
Extract products can be described as complementing the
nutritive properties of peptone by contributing minerals,
phosphates, energy sources and those essential factors missing
from peptone.8
Typical Analysis
Refer to Product Tables in the Reference Guide section of this
manual.
Directions for Preparation from
Dehydrated Product
Refer to the final concentration of Beef Extract Powder,
Beef Extract, Desiccated or Beef Extract in the formula of the
medium being prepared. Add appropriate product as required.
Procedure
See appropriate references for specific procedures using
Beef Extract Powder, Bacto Beef Extract, Desiccated or Beef
Extract.
Expected Results
Refer to appropriate references and procedures for results.
73
3/16/09 3:46:21 PM