Operation Instructions and Precautions

Precautions:
1. Power Requirements
A. Make sure the analyzer is properly grounded.
B. Only install fuses of specified specification and use original power cord.
C. Before turning on the analyzer, make sure the input voltage meets the requirements.
2. General Environment
A. Optimal operating temperature: 15 ℃ - 30 ℃;
B. Running temperature: 10 ℃ - 40 ℃;
C. Optimal operating humidity: 20 % - 85 %;
D. Atmospheric pressure: 70 kPa - 106 kPa;
E. The environment should be as free as possible from dust, mechanical vibrations, loud noises,
pollution and electrical interference.
F. It is advisable to evaluate the electromagnetic environment prior to the operation of this analyzer;
G. Do not use this analyzer in close proximity to sources of strong electromagnetic radiation;
H. Do not place the analyzer near brush-type motors, flickering fluorescent lights, and electrical
contacts that regularly open and close;
I. Do not place the analyzer in direct sunlight or in front of a source of heat or drafts;
J. The environment should have good ventilation;
K. Make sure the analyzer is properly grounded;
L. Do not place the analyzer on a slope. The analyzer shall be used indoor.
3. Troubleshooting
In case of any error, click the error message area at the bottom right of the screen for troubleshooting information.
See Chapter 11 Troubleshooting Your Analyzer of the operator’s manual for details.
4. Daily Maintenance
Daily: Shut down your analyzer as instructed and perform probe cleanser maintenance.
Monthly: The surface and black plate of the probe wipe shall be wiped every month. (It is recommended to use
alcohol whose concentration is 75%).
If the analyzer is free from using for a long time (longer than one week), you should perform the “Prepare to ship”
procedure and shut down the analyzer.
1. Preparations before turning on the analyzer
Check and make sure the reagents are enough and not turbid. Check and make sure the tubes are not bended.
Check and make sure the external devices are connected to the analyzer properly.
Check and make sure the power cord is connected properly.
2. Turning on the analyzer and user login
Switch on the printer if the analyzer is connected to the printer. Then power on the analyzer by switch on the
analyzer at the back. Enter the user name and the password into the pop-up dialog box to log in the main screen.
3. Background test
Background test will be performed automatically after the analyzer is powered on. Enter the “Sample Analysis”
screen and then check whether the displayed background results meet the following requirements:
WBC≤0.20 × 10 / L
9 12 9
RBC≤0.02×10 /L HGB≤1g/L PLT≤10×10 /L HCT≤0.5%
At least one background test will be performed during the power-on procedure. If the results of the first
background test fail, another background test will be performed. If the results still fail to meet the requirements,
the “Background Abnormal” error will be prompted. Perform troubleshooting as instructed in the
Troubleshooting chapter of the operator’s manual.

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4. Daily Controls
Perform the QC programs daily to monitor the analyzer status. (See Chapter 8 Using the QC Programs of the
operator’s manual for details.)
5. Selecting the work mode
A. At the “Sample Analysis” screen, click the “Mode” button and the “Mode” dialog box will pop up.
B. Click the “WB -CBC+DIFF”, “WB –CBC”, “Predilute -CBC+DIFF” or "Predilute –CBC” radio button to
select the desired work mode.
C. There are two sample entry methods for the samples to be analyzed: enter the sample ID and enter all
the information.
D. Click “OK” to save the setting and return to the “Sample Analysis” screen.
6. Whole blood mode
A. Well mix the sample anticoagulated with the EDTAK2 or EDTAK3. Then, present the sample to the
sample probe and make sure the probe is dipped into the blood.
B. Press the aspirate key and the analyzer will aspirate sample automatically. When you hear the beep,
remove the sample tube. The analyzer will automatically run the sample.
7. Predilute mode
A. Click the “Diluent” button of the status bar at the top of the analyzer. Then, according to the prompt on
the screen, present a clean uncapped centrifugal tube without anticoagulant to the sample probe
vertically. Make sure the sample probe reaches the bottom of the tube.
B. Press the aspirate key to dispense the diluent. During the dispensing process, you should be careful to
avoid bubbles, hangings and spills. When you hear the beep, remove the tube. You can also dispense
480µL of diluent by pipette into the tube.
C. When finish dispensing the diluent, you can click the “Cancel” button to exit the procedure. The
dialogue will be closed automatically.
D. Manually collect and dispense 20µL blood sample into the centrifugal tube with diluent. Then, cap the
tube and mix the sample well.
E. Keep this prediluted sample still for 5 minutes and then mix it again. Then, present the sample to the
sample probe and press the aspirate key. The analyzer will aspirate the sample automatically. When
you hear the beep, remove the sample tube. The analyzer will automatically run the sample.
8. Sleep
The analyzer will enter the standby mode if relevant fluidic operations have stopped for a period out of the set
interval. During this condition, during this condition, you can not perform any operations that relevant with fluidic
operations.
When the analyzer is in the sleep mode, you can press the aspirate key to exit the sleep mode.
9. Shutdown
After the analysis, click the Shutdown button at the main menu to shutdown the analyzer daily and perform probe
cleanser maintenance as instructed.
When it prompts “Are you sure to shut down the analyzer?” click “OK” and then power off the analyzer and exit the
software. Switch off all other external equipment.
Empty the waste container and dispose of the waste properly.

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