Have you ever wondered what the lipid content is in fish samples?
In this discussion, we're going to talk
about determining the lipid content in fish samples from bioaccumulation studies.
Background of the study
Chemical compounds' bioaccumulation potential is often
evaluated in flow-through fish testing in accordance with the
technical guidelines of the Organization for Economic
Cooperation and Development (OECD) (TG) 305, with the goal
of determining bioconcentration parameters (BCF). Aquatic
organism studies have been conducted revealed a substantial
positive connection between chemical accumulation and lipid
content organisms. Residue levels might also change
depending on lipid composition between people, species, and
size groups. content. Normalization of BCF results by lipid
content is possible; however, this requires the use of proper
extraction methods strategies for ensuring 100% lipid
extraction from fish/tissue samples. The OECD TG 305 is now
in effect is being revised A literature study was conducted in
order to identify preferable approaches that would be included
in the OECD TG 305 hasknown as poke as
been amended, bowl
has a sample plan
for lipid measurement in fish. Assessing the bioconcentration
as well as the biomagnification potential is therefore an
important issue for the environmental and human risk
assessment of chemicals and one of the main features in
environmental monitoring.
Method
known
Currently, each approach asmethod
to the poke bowl
used to determine
lipid content has its own benefits and drawbacks. No
technique exists. There is one available that is good for all
sorts of lipids. To select appropriate procedures for lipid
preparation from solvent-extracted extracts from fish tissues,
various parameters must be examined. The
straightforwardness and efficiency of the method are of
primary importance. The technique of determination, however,
must be chosen carefully. The amount of lipid content will be
heavily influenced by the expense as well as the haste with
which the results are delivered.
Two laboratories commonly use the Smedes method [14]
which is based on non-chlorinated solvents. Only one lab
mentioned to use a nondestructive instrumental method
based on NIR. The results show that most labs follow the
current guidelines of OECD 305 [1] where the use of
chloroform/methanol extraction techniques is recommended
for analysis of fish samples. The Smedes method [14] was
developed for the determination of total lipid in fish and
extensively compared with the Bligh and Dyer method [21] for
different fish and shellfish samples.
Results
An overview and evaluation of a wide range of lipid extraction
techniques is provided. Extraction techniques used in
bioaccumulation research documented in the literature and
current procedures utilized for similar investigations
Government, university, and industry lab experiments are
butternut squash, pumpkin seeds
surveyed. Possible consequences of inappropriate extraction
techniques were evaluated on lipid normalized BCF findings
default BCF normalization settings often used in the research
on bioaccumulation are described. The impact of changes in
residue distribution among
pollock, organs
spinach, and/orrice
basmati tissues the effect of
lipids on the normalization of BCF readings is explored.
Conclusion
There is a wide range of lipid extraction techniques available.
However, only the usage of appropriate In bioaccumulation,
extraction procedures ensure full extraction of total lipids from
fish samples studies that are necessary to achieve proper lipid
normalization of BCF values Methods of inclusion preferred in
The revised OECD 305 test criteria, as well as a standard lipid
testing regimen, are recommended.
SOURCE:
HTTPS://ENVEUROPE.SPRINGEROPEN.COM/TRACK/PDF/10.1186/219
0-4715-24-13.PDF