NATIONAL INSTITUTE OF TECHNOLOGY: SILCHAR DEPARTMENT OF CHEMISTRY ~ B.tech practical lab Subject code: CH1102 xP NT o \ ESTIMATION CALCIUM IN MILK POWDER BY EDTA PROCEDURE: Title: Pipette out 25m! of milk solution into a 250 ml conical flask, Dilute it by 25m! eae tt oe ead 2inl buffer solution and Im Mg-EDTA solution followed by 3-4 drops of Erichrome-Black T indicator. Titrate the resulting solution with standard corso eion until the wine red solution changes to clear blue which is the end. point of the titration. Titrate until a concordant reading is obtained. TABLE Nor}-Vorume of | Burette reading | Vol of EDTA] Concordart of supplied ; : consumed | Volume of 62" )| casbhution,, “(no Piney (ml) EDTA (ml) fall \ Required Data- ea iy Strength of supplied EDTA Solution / i) 1ml0.01 MEDTA Solution =0.01M = 0.4008 mg of Ca. i Céleulate the amount of Calcium present in 1000 mil Solution of Ca: . | : CALCULATION: Iml 0.01 M EDTA solution = 0.4008 mg of Ca) 2 Y ml 0.01M EDTA solution x 0.4008 mg of Ca ‘ 4 ‘amount of CalciumiLir = Y0.4008x1000 ppm (mg/Ltr.) 25 Conclusion: -ppm(mg/Ltr.) Oe Amount of Calcium present \ Scanned with CamScanner\A ven NS RNS SN ss SEEN \. NATIONAL INSTITUTE OF ‘TECHNOLOGY sULCHAR DEPARTMENT OF CHEMISTRY (1) PRESENT IN STANDARD KMn04 SOLUTION Nai - ‘TITLE: TO DETERMINE ‘THE‘AMOUNT.OF IRON SOLUTION USING iron (M1) solution in a 500ml conical flask. Add 20m (one test ‘olution with stand: ‘Pitrate the resulting s ipette out 25 ml the titration. PROCEDURE: Pit aeid and phosphoric acid mixture. 7 ul JahdnOx solution until a fant pink color just appe=tS which is end point of Repeat the titration to get the concordant reading 5 TABLE = a FiocofOts [Volume of | Burette readin Yotume of KMnO. | Concordant Iron(t) Initial Final used (ml) volume (eal) solution (ml) CALCULATION Given that strength of KMn0Os solutioy NKMn0g solution 1 mlof. KMn0s solution = 0.0005585 gm of iron “Amount of Iron (11) in g/L = sibO5585 %¥.<2000 25 oS CONCLUSION: Amount of Iron in 1 L of supplied solution = we Scanned with CamScanner6: B.toch practical tab Subject code: cH1102 “ : ERINENT NO.6 ’ ‘tle: TO DETERMINE THE STR) : RENGTH OF STANDARD ON RHE STRENGTH OF THE any ae usING pee ob) ROCEDURE: Pipette out 25ml oxalic aci ion in'a conic sk, Add 21 one. HaSOs followed by Soe oat Heid solution in'a conical Mask, Add 2m, Beat the solition to $0-60°C temperature and titrate this hot solution with KMnOs tre sop ken in a burette til a faint pink colour persists for about 30s, which is ¢ end point of the titratign, Repeat the titration to get concordant reading. TABLE No. of [Volume of Burrete” | Vol. of KMnOy | Concordant obs. | oxalic acid reading required(ml) | volume(ml) taken (ml) . initial | Final CALCULATION: 8 “% Vi ——+ _ Vol of Oxalic acid solution taken, Si: ——+ _ Strength of oxalié acid solution, ——* Concordant volume of KMnO, solution. Ve . os 82 > Strength of KMinOs solution. . Xe J od EOLLOWING THE FORMULA ' Vi Sie V2S2_ “ 7 Strength of KMnOs solution S2= YS oN s Calculation of amount of Manganese (KMnO« in gmi/L = strength of KNnO4 X qv. Wt. of KMnOs " eToys? = SoXBl 5 . : = W-gram. p (ii) Amount of Manganese in gm/L=48x W'gm . 158 a ' — Scanned with CamScannerip) ‘ NATIONAL INSTITUTE OF TECHNOLOGY: SILCHAR DEPARTMENT OF CHEMISTRY é DEPARTMENT OF CHEMISTRY B.tech practical lab Subject code: CH1102 iy EXPERIMENT NO. ‘Title, DETERMINATION OF DISSOLVED OXYGEN (D.0] OF WATER PROCEDURE: ‘Take $8 ml. water sample (lake/pond/tap) in a conical solution and’ ml alkaline KI solution to it. Shake the flaskend cover the mouth of the flask with a paper. Keéep the flask for 5 minutes ‘for settling the brown precipitate. Add 1-2 ml. conc, HoS0, and shaktvell to-dissolve the precipitate, — ‘The solution-becomes brown. Titrate this solution wyth the standard Hypo (Na2S203) solution. When the brown colour changés to pale yellow add 1-2 ml starch (1%) solution to it. Continue the titration till the dark brown/violet colour changes to. colourless which is the end point of the titration. Repeat the titration to get concordant volume. Calculate the amount of dissolved oxygen in ppm. fe flask, Add 1 ml MnSOs Given, the strength of Hypo solution= 0.01 N TABLE ee oes ‘iE pens Ne. iments of Burette reading: Vol. of Hypo =] f Concordant of ake/supplied - consumed’(ml) | Volume of obs. | solution taken (om) | Initial | Final Hypo * (ml) i b> 1 2. Zin 3. \ \ Calculation: 1000 ml 1 N Hypo (NasS20,) solution = 8 gms of cxygen te | __ Em. LN Hypo (War8203) sotution 8° _gms of Oxygen 2 i + 4000 ml 1N Hypo (WaaS203) solution 8 __x 1000 mg of Oxygen’ + 1060) . ae 0.01 N Hypo (Na2S203) solution = 8x 0.01 x Z mg of Oxygen issolved Oxygen/L = _8x0.01 x Zx 1000 ppm - ppm. 100 ml . 1 Conclusion: - :- Amount of Dissolved Oxygen = ____ mg/L (ppm) “a PF sec" oe. 2 5 a Scanned with CamScanner qNATIONAL INSTITUTE OF TECHNOLOGY: SILCHAR NATIONAL INSTITUTE OF TECHNOLOGY: SILCHAR DEPARTMENT OF CHEMISTRY e Btech practical lab Subject code: CH1102 ’ : (PERIMENT NO.3 y ESTIMATION CALCIUM EDT, PROCEDURE: Titk Pipette out 25ml of Solution into a 250 ml conical flask, Dilute it iy zeml water. : en add 2ml buffer solution and lmi{Mig-EDTA solution followe 2y 3-4 drops of Exichrome-Elack T indicator Titrate the resulting solution with standard EDTA Sclution until the wine red Solution changes to clear blue which is the end point of € titration, Titraté until a concordant Teading is obtained, TABLE Volume of Burette reading. Vol. of EDTA Concordant 5 gromiied | iaitll | Final] - consumed -| yarream Ca®solution | Initial [Final (ml) EDTA (ml) taken (ml) Y | ™ Required Data- 4) Strength of supplied EDTA Solutiva = 0.01M 4 i) 1ml 0.01 M EDTA Solution = 0.4008 mg of Ca. Calculate the amount of Calcium present in 1000 ml Solution of Ca, CALCULATION: Iml 0.01 M EDTA solution 4008 mg of Ca ¥ml0.01 MEDTA solution =x 0.4008 mg of Ca Y 2 Amoint of CalumLtr = Yx0.4008 x 4009 4PM (mgt) 25 ‘ Conclusion: Amountof Calcium present = Ppm(mg/Ltz,) 3 ae => . N\ Scanned with CamScannery + NATIONALINSTITUTIt OF THCHNOLOGY: SILCHAR DE} EXPERIMENT NO,2 | NT_OF CHEMISTRY Subject code: CH11 PROCEDURE: Pipotte out 25-ml of Mg- solution in w conical flask, Add 50 ml, distilled water (3 test |, full), 2 ml, buffer, solution and 2-3 drops Erlo-Chrome Black ‘T indicator, ‘Titraté” | resulting solution with standard EDTA solution till the wine-red colour of the solu changes to clear blue, Note down the end-point arid repeat the titration till concor volume is obtained. TABLE No, Volume of Burote eading [Vol of EDTA [Concordant of ob | Inke/supplied Mg ~ = consumed (ml) | Volume of Solution taken. ‘eh Initial] \\Final EDTA (ml) | sj . a 2. Yami 3. I DAT) > Strength of the supplied’ EDTA SOLUTION = 0.01'M, > 1 ml. 0.01 MEDTA SOLUTION Calculate the amount of magnesium in mg. present in 1000 ml of solution of Mg CALCULATION: 5 0.2432 mg. Magnesium 1000 ml, 1 M EDTA solution is equivalent to = 24.32 gm of Magnesium = 24.32 gm of Magnesium 1 ml, 1 M EDTA solution is equivalent to - 1 ml 0.01 M EDTA solution is equivalent to 1m! 0.01°M EDTA Solution is equivalent to 1000 = 24.32 x0.01 gm of Magnesium 1000 © 24.32 x0.01 x1000 mgm of Mg. 1000 = 0.2432 Nig of Magnesium ‘Therefore, ¥ ml. 0.01 M solution is equivalent = Y x 0.2432 mg of Magnesium. ‘Therefore, Amount of Magnesium in mg /Itr, = ¥.x0,2432 x.1000 ppm (mg/Itr) 25 Conclusion: Amount of Magnesium = ---- Scanned with CamScannerNATIONAL INSTITUTE. OF TECHNOLOSS: SILCHAR, STMENT OF CHEMISTRY . ‘DEPAR’ B.tech practical Iab Subject code:CH1102 EXPERIMENT NO.1 site: 10 DETERMINE THE TOTAL HARDEEES OF POND WATER /SUPPLIED WATER USING STANDARD EDTA SOLUTION PROCEDURE ‘MEASURE 100 ml, lke/supplied water Using a mens cylinder and take in & apo al, conical Gani. 00d 23 ml bales Son, ‘emmonium chloride and 250/500 mi, Sgronide of P= 10) followed by, SA ork ‘of Eriochrome Black-T ammonin Pye uns solution with standard EDTA Se tll colour changes from indicator Weiter blac, which i ehe end point of the Uealons Repeat the titration Ul concordant reading is obtained. Given 1) Strength of EDTA solutions 0.01 10 IMS inLIM EDTA solution = 100/1000 em of CacOs TABLE a ‘Volume of Surcie reading | Vol of EDTA ] Concordant ‘consumed | Volume of (al) EDTA (mi) Wo. ‘of | lake/supplicd solution taken (el) T 2. 3. CACULATION: 1ml.~ 1M EDTA solution js equivalent to = 0.1 gm of CaCOs 1 mi, 0.01 MEDTA solition is equivalent to = 0.1 x 0,01 g of CaCOs 1 ml. 0.01 MEDTA solution is equivalent to = 0.1 x 0.04 x 1000 mg of Cas ! = 1 mg of CacQs 4m, 0.01 M EDTA solution is equivalent to 1XX m6 of cacds ‘Therefore, total hardness of water =X x 1000/ 100 ppm of CaCOs Conclusio ‘Total hardness of Pond water /supplied water “ppm Scanned with CamScanner