Republic of the Philippines

Department of Agriculture
Bureau of Fisheries and Aquatic Resources
NATIONAL INTEGRATED FISHERIES TECHNOLOGY DEVELOPMENT CENTER
MICROBIOLOGY AND FISH HEALTH LABORATORY
Bonuan Binloc, Dagupan City, Pangasinan, Philippines
Tel. Nos. (075) 649-6086; (075) 649-6068
Email address: bfarniftdc@yahoo.com / bfarniftdc.microlab@gmail.com

CLINICAL LABORATORY RECORD

Case Code No : AAHL-04-13-2021-007 Date & Time Submitted:04/13/21|02:20 PM

Sample Code No. : LA-FHL-010 Date & Time Examined:04/13/21|02:25 PM
Sample(s) : 1 Siganid breeder
Date &Time Collected: April 13, 2021 | 02:00 PM
Place Collected/Source: Siganids Hatchery Section – Experimental Tank
Submitted by : Ms. Shaena Mae Mahusay
Company Name : National Fisheries Research Development Institute
Address : BFAR – NIFTDC Compound, Bonuan Binloc, Dagupan City, Pangasinan, 2400
Analysis Conducted : Gross External and Internal Examination (Necropsy) | Parasite Examination

Laboratory Findings:
Necropsy:
- Eyes were clear and apparently normal
- Abnormal body color but no signs of inflammation or bruises were observed on
the skin.
- Bright-red gills and brownish colored liver were also observed.

Parasitological:
- Wet mounts of gills, intestines and other vital organs were prepared and
subjected for microscopy. Upon microscopic examination, presence of gill flukes
identified as Dactylogyrus spp. were seen on the gill filaments of the Siganid
breeder. In addition, presence of roundworms or nematodes were also seen in the
gastro-intestinal tract and identified as Camallanus spp.

A B
Figure 1. (A) Examined Siganid breeder exposing gastro-intestinal structures with nematodes. (B) microscopic view of an
adult nematode identified as Camallanus spp. (Magnification – 40x)

Result(s) as per sample(s) received and analyzed. This report shall not be reproduced except in full and with written
consent from BFAR-NIFTDC Microbiology and Fish Health Laboratory Section

Examined by: Checked by: Noted by:

JOHN CARLO G. GOSILATAR EDITH C. ROXAS CORDELIA B. NIPALES

Laboratory Analyst Senior Aquaculturist Supervising Aquaculturist
Head, Microbiology and Officer-In-Charge, BFAR -
RIZALYN J. BAUTISTA Molecular Pathology NIFTDC
Laboratory Analyst Laboratory
Head, Microbiology and Aquatic
GEELYN DV. CAROLINO
Laboratory Analyst
 Republic of the Philippines
Department of Agriculture
Bureau of Fisheries and Aquatic Resources
NATIONAL INTEGRATED FISHERIES TECHNOLOGY DEVELOPMENT CENTER
MICROBIOLOGY AND FISH HEALTH LABORATORY
Bonuan Binloc, Dagupan City, Pangasinan, Philippines
Tel. Nos. (075) 649-6086; (075) 649-6068
Email address: bfarniftdc@yahoo.com / bfarniftdc.microlab@gmail.com

CLINICAL LABORATORY RECORD

Case Code No : AAHL-04-14-2021-008 Date & Time Submitted:04/14/21|10:10 AM

Sample Code No. : LA-FHL-011-012 Date & Time Examined:04/14/21|10:15 AM
Sample(s) : 2 Siganid breeders (Siganus guttatus: fed with fresh diet for one (1) month and
Siganus vermiculatus: continuously fed with fresh diet)
Date &Time Collected: April 14, 2021 | 10:00 AM
Place Collected/Source: Siganids Hatchery Section – Experimental Tank
Submitted by : Ms. Renalee B. Arenas
Company Name : National Fisheries Research Development Institute
Address : BFAR – NIFTDC Compound, Bonuan Binloc, Dagupan City, Pangasinan, 2400
Analysis Conducted : Gross External and Internal Examination (Necropsy) | Parasite Examination

Laboratory Findings:
Necropsy:
- Results of necropsy showed that eyes were clear and white patches on the body
but with no signs of inflammation or bruises were observed on the skin for both
Siganid breeder samples submitted.
- Brownish colored liver was observed on Siganus vermiculatus and apparently
normal light red colored liver was observed on Siganus guttatus.

Parasitological:
- Wet mounts of gills, intestines and other vital organs were prepared and
subjected for microscopy. Upon microscopic examination, presence of gill flukes
identified as Dactylogyrus spp. were seen on the gill filaments of the Siganid
breeder. In addition, presence of roundworms or nematodes were also seen in the
gastro-intestinal tract and identified as Camallanus spp.. Parasites identified
were seen on both Siganid breeders samples submitted.

D
Figure 2. (C) Nematodes or roundworms invaded the intestinal tissues of both Siganid
A B
breeders. (B) Microscopic view of Dactylogyrus spp. seen in gills of both Siganid
breeders.
Figure 1. Examined – Magnification
Siganid Breeders. (A)–Siganus
100x. guttatus continuously fed with fresh diet – Total Length: 23.5 cm
(B) Siganus vermiculatus fed with fresh diet for one (1) month – Total Length: 24 cm
 Result(s) as per sample(s) received and analyzed. This report shall not be reproduced except in full and with written
consent from BFAR-NIFTDC Microbiology and Fish Health Laboratory Section

Examined by: Checked by: Noted by:

JOHN CARLO G. GOSILATAR EDITH C. ROXAS CORDELIA B. NIPALES

Laboratory Analyst Senior Aquaculturist Supervising Aquaculturist
Head, Microbiology and Officer-In-Charge, BFAR -
RIZALYN J. BAUTISTA Molecular Pathology NIFTDC
Laboratory Analyst Laboratory
Head, Microbiology and Aquatic
GEELYN DV. CAROLINO
Laboratory Analyst

Republic of the Philippines

Department of Agriculture
Bureau of Fisheries and Aquatic Resources
NATIONAL INTEGRATED FISHERIES TECHNOLOGY DEVELOPMENT CENTER
MICROBIOLOGY AND FISH HEALTH LABORATORY
Bonuan Binloc, Dagupan City, Pangasinan, Philippines
Tel. Nos. (075) 649-6086; (075) 649-6068
Email address: bfarniftdc@yahoo.com / bfarniftdc.microlab@gmail.com

CLINICAL LABORATORY RECORD

Case Code No : AAHL-04-15-2021-009 Date & Time Submitted:04/15/21|09:30 AM

Sample Code No. : LA-FHL-013 Date & Time Examined:04/15/21|09:33 AM
 Sample(s) : 1 Siganid breeder (Siganus guttatus)
Date &Time Collected: April 15, 2021 | 09:00 AM
Place Collected/Source: Siganids Hatchery Section – Broodstock Tank
Submitted by : Mr. Christopher Laban
Company Name : BFAR – NIFTDC (Siganids Hatchery Section)
Address : BFAR – NIFTDC Compound, Bonuan Binloc, Dagupan City, Pangasinan, 2400
Analysis Conducted : Gross External and Internal Examination (Necropsy) | Parasite Examination

Laboratory Findings:
Necropsy:
- Eyes were clear and apparently normal
- Abnormal body color but no signs of inflammation or bruises were observed on
the skin.
- Gills and other vital organs including liver and gastro-intestinal were apparently
normal in appearance.

Parasitological:
- Wet mounts of gills, intestines and
other vital organs were prepared and
subjected for microscopy. Upon
microscopy, presence of roundworms
or nematodes were also seen in the
gastro-intestinal tract and identified
as Camallanus spp.

Figure 1. Examined Siganid (Siganus guttatus) breeder

exposing gastro-intestinal structures with nematodes.

Result(s) as per sample(s) received and analyzed. This report shall not be reproduced except in full and with written
consent from BFAR-NIFTDC Microbiology and Fish Health Laboratory Section

Examined by: Checked by: Noted by:

JOHN CARLO G. GOSILATAR EDITH C. ROXAS CORDELIA B. NIPALES

Laboratory Analyst Senior Aquaculturist Supervising Aquaculturist
Head, Microbiology and Officer-In-Charge, BFAR -
RIZALYN J. BAUTISTA Molecular Pathology NIFTDC
Laboratory Analyst Laboratory
Head, Microbiology and Aquatic
GEELYN DV. CAROLINO
Laboratory Analyst