Republic of the Philippines

Department of Agriculture
Bureau of Fisheries and Aquatic Resources
NATIONAL INTEGRATED FISHERIES TECHNOLOGY DEVELOPMENT CENTER
MICROBIOLOGY AND FISH HEALTH LABORATORY
Bonuan Binloc, Dagupan City, Pangasinan, Philippines
Tel. Nos. (075) 649-6086; (075) 649-6068
Email address: bfarniftdc@yahoo.com / bfarniftdc.microlab@gmail.com

CLINICAL LABORATORY RECORD

Case Code No : AAHL-04-20-2021-015 Date & Time Submitted:04/20/21|02:28

PM
Sample Code No. : LA-FHL-015-017 Date & Time Examined:04/20/21|02:35
PM
Sample(s) : 3 water samples
Date &Time Collected: April 20, 2021 | 01:25 PM
Place Collected/Source: Macrobrachium Hatchery broodstock, larval rearing tank and reservoir
Submitted by : Mr. Jomar DV. Parana
Company Name : BFAR – NIFTDC – Macrobrachium Hatchery Section
Address : BFAR – NIFTDC Compound, Bonuan Binloc, Dagupan City, Pangasinan, 2400
Analysis Conducted : Aerobic Plate Count (cfu/ml) | Presumptive (Total) Vibrio Count (cfu/ml) |
Luminous Bacterial Count (cfu/ml) and Bacterial Identification

RESULT OF BACTERIOLOGICAL ANALYSIS

A. Result of Bacterial Enumeration

Total
Percentage of
Aerobic Plate Count (Presumptive) Percentage of Green Luminous
Sample Code Source Yellow Vibrio
(cfu/ml) Vibrio Count Vibrio Colonies (%) Bacterial (cfu/ml)
Colonies (%)
(cfu/ml)
Broodstock Tank
LA-FHL-015-FW <2.5 x 103 0 0% 0% ND
(Gene Bank)
Brackishwater
LA-FHL-016-BW <2.5 x 103 0 0% 0% ND
(mixing tank #03)
LA-FHL-017-SW Seawater (reservoir) 9 x 103
6 x 10 2
0% 100% ND
METHODOLOGY: Serial Dilution Method (Spread Plate)

STANDARD BACTERIAL PROFILE (Bacterial Analysis for Shrimp and Water: W1-BA-01 – BFAR CO)
Total Bacterial Count <5.0 x 106 - NORMAL
5.1 x 106-8.9 x 106 - Slightly high
9.0 x 106-5.0 x 107 - High
5.1 x 107-9.0 x 107 - Very high
9.1 x 107-1.5 x 107 - Extremely high

Note: For shrimp and pond water, percentage of yellow Vibrio colonies should be more than 60%
ND – means Not Detected
TNTC – means Too Numerous to Count

Remarks:
All samples were found to have Aerobic Plate Count (cfu/ml) and Total Vibrio Count
(cfu/ml) the normal range based on standard bacterial profile for shrimp and water: W1-BA-01-BFAR
CO.
 B. Result of Bacterial Identification

Specimen Bacterial Isolate

Freshwater Sphingomonas paucimobilis
Brackishwater Vibrio cholerae
Vibrio cholerae
Seawater
Vibrio alginolyticus
METHODOLOGY: Bacterial Identification using API 20E

Result(s) as per sample(s) received and analyzed. This report shall not be reproduced except in full and with
written consent from BFAR-NIFTDC Microbiology and Fish Health Laboratory Section

Examined by: Checked by: Noted by:

JOHN CARLO G. GOSILATAR EDITH C. ROXAS CORDELIA B. NIPALES

Laboratory Analyst Senior Aquaculturist Supervising Aquaculturist
Head, Microbiology and Officer-In-Charge, BFAR - NIFTDC
Molecular Pathology Laboratory
RIZALYN J. BAUTISTA Head, Microbiology and Aquatic
Laboratory Analyst Animal Health Laboratory

GEELYN DV. CAROLINO

Laboratory Analyst